A competitive precision CRISPR method to identify the fitness effects of transcription factor binding sites

Here we describe a competitive genome editing method that measures the effect of mutations on molecular functions, based on precision CRISPR editing using template libraries with either the original or altered sequence, and a sequence tag, enabling direct comparison between original and mutated cell...

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Veröffentlicht in:	Nature biotechnology 2023-02, Vol.41 (2), p.197-203
Hauptverfasser:	Pihlajamaa, Päivi, Kauko, Otto, Sahu, Biswajyoti, Kivioja, Teemu, Taipale, Jussi
Format:	Artikel
Sprache:	eng
Schlagworte:	631/1647/1511 631/208 631/337/572 Agriculture Binding sites Binding Sites - genetics Biochemistry Bioinformatics Biomedical and Life Sciences Biomedical Engineering/Biotechnology Biomedicine Biotechnology Brief Communication Cell cycle Clustered Regularly Interspaced Short Palindromic Repeats - genetics Competition CRISPR CRISPR-Cas Systems - genetics DNA damage Editing Fitness Gene Editing - methods Gene expression Gene loci Genome editing Genomes Genomics Identification methods Life Sciences Medical research Medicin och hälsovetenskap Mutation Myc protein Promoter Regions, Genetic - genetics Reproductive fitness Transcription factors Transcription Factors - chemistry Transcription Factors - genetics
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