Allergen challenge-induced entry of α 2-macroglobulin and tryptase into human nasal and bronchial airways
Background: Microvascular-epithelial exudation of bulk plasma may characterize inflammatory airway diseases. This study compares the acute allergen challenge–induced mast cell and exudative responses in nasal and bronchial airways. The focus is on α 2-macroglobulin as an index of luminal entry of pl...
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| Veröffentlicht in: | Journal of allergy and clinical immunology 1995, Vol.96 (2), p.239-246 |
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| creator | Svensson, Christer Grönneberg, Reidar Andersson, Morgan Alkner, Ulf Andersson, Olof Billing, Bo Gilljam, Hans Greiff, Lennart Persson, Carl G.A. |
| description | Background:
Microvascular-epithelial exudation of bulk plasma may characterize inflammatory airway diseases. This study compares the acute allergen challenge–induced mast cell and exudative responses in nasal and bronchial airways. The focus is on α
2-macroglobulin as an index of luminal entry of plasma exudates.
Methods:
Separate nasal and bronchial allergen challenges were carried out outside the pollen season in eight patients with pollen-induced seasonal allergic rhinitis. The levels of different-sized plasma proteins (albumin molecular weight, 66,000 d and α
2-macroglobulin molecular weight, 725,000 d) and tryptase were determined in pre- and postchallenge nasal lavage and bronchoalveolar lavage (BAL) fluids. Diluent and increasing doses of allergen were sprayed into the right nasal cavity, and each challenge was followed by a nasal lavage (volume, 15 ml) with a “nasal pool” device (recovery, >80%). Endobronchial allergen challenge (individual doses) and BAL (volume, 2 × 25 ml) were performed in a lobe bronchus through a fiberoptic bronchoscope (recovery, 30%). Saline challenge and BAL were carried out in the contralateral lung as control.
Results:
The levels of albumin, α
2-macroglobulin, and tryptase increased dose-dependently in postchallenge nasal lavage fluids (
p < 0.05) and correlated to nasal symptoms. In particular, albumin and α
2-macroglobulin correlated (
r = 0.98,
p < 0.001). Both α
2-macroglobulin and tryptase, but not albumin, were increased in BAL fluids from the allergen-challenged side (
p < 0.05).
Conclusion:
Local allergen challenge causes luminal entry of tryptase and α
2-macroglobulin in the nose and bronchi of patients with allergy. We suggest that mast cell and plasma exudation responses may be similar in human nasal and bronchial airways and that albumin levels (in BAL fluids) may not well reflect the exudation process in bronchial airways. (J A
LLERGY C
LIN I
MMUNOL 1995;96:239-46.) |
| doi_str_mv | 10.1016/S0091-6749(95)70013-7 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_swepu</sourceid><recordid>TN_cdi_swepub_primary_oai_swepub_ki_se_444628</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0091674995700137</els_id><sourcerecordid>77442221</sourcerecordid><originalsourceid>FETCH-LOGICAL-e1958-e3cef635722f248a6f9c416373b2b26cb2dc3a4f2d05d37f539d9edf64cb5c443</originalsourceid><addsrcrecordid>eNp1kU1uFDEQhS0ECkPgCJG8QrAw-LfdXqEoIoAUiQWwttx29YxDt3uwuxPNsXIRzoTnh7Bi5ap679klfwhdMPqOUda8_0apYaTR0rwx6q2mlAmin6AVo0aTpuXqKVo9Wp6jF6Xc0tqL1pyhM62kUFSs0O3lMEBeQ8J-42qZ1kBiCouHgCHNeYenHv9-wJyMzudpPUzdMsSEXQq4qtvZFcAxzRPeLKNLOLnihoPa5Sn5Tdx3Md-7XXmJnvVuKPDqdJ6jH9cfv199JjdfP325urwhwIxqCQgPfSOU5rznsnVNb7xkjdCi4x1vfMeDF072PFAVhO6VMMFA6BvpO-WlFOeIHO8t97BdOrvNcXR5ZycX7Wn0s1ZgpZQNb6tf_9e_zVP4F_obZKauZ0RNvj4mq-3XAmW2YywehsElmJZitZaSc86q8eJkXLoRwuMTJwxV_3DUof7LXYRsi4-QKoSYwc82TNEyavfc7YG73UO1RtkDd6vFHyjUooM</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>77442221</pqid></control><display><type>article</type><title>Allergen challenge-induced entry of α 2-macroglobulin and tryptase into human nasal and bronchial airways</title><source>MEDLINE</source><source>Access via ScienceDirect (Elsevier)</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Svensson, Christer ; Grönneberg, Reidar ; Andersson, Morgan ; Alkner, Ulf ; Andersson, Olof ; Billing, Bo ; Gilljam, Hans ; Greiff, Lennart ; Persson, Carl G.A.</creator><creatorcontrib>Svensson, Christer ; Grönneberg, Reidar ; Andersson, Morgan ; Alkner, Ulf ; Andersson, Olof ; Billing, Bo ; Gilljam, Hans ; Greiff, Lennart ; Persson, Carl G.A.</creatorcontrib><description>Background:
Microvascular-epithelial exudation of bulk plasma may characterize inflammatory airway diseases. This study compares the acute allergen challenge–induced mast cell and exudative responses in nasal and bronchial airways. The focus is on α
2-macroglobulin as an index of luminal entry of plasma exudates.
Methods:
Separate nasal and bronchial allergen challenges were carried out outside the pollen season in eight patients with pollen-induced seasonal allergic rhinitis. The levels of different-sized plasma proteins (albumin molecular weight, 66,000 d and α
2-macroglobulin molecular weight, 725,000 d) and tryptase were determined in pre- and postchallenge nasal lavage and bronchoalveolar lavage (BAL) fluids. Diluent and increasing doses of allergen were sprayed into the right nasal cavity, and each challenge was followed by a nasal lavage (volume, 15 ml) with a “nasal pool” device (recovery, >80%). Endobronchial allergen challenge (individual doses) and BAL (volume, 2 × 25 ml) were performed in a lobe bronchus through a fiberoptic bronchoscope (recovery, 30%). Saline challenge and BAL were carried out in the contralateral lung as control.
Results:
The levels of albumin, α
2-macroglobulin, and tryptase increased dose-dependently in postchallenge nasal lavage fluids (
p < 0.05) and correlated to nasal symptoms. In particular, albumin and α
2-macroglobulin correlated (
r = 0.98,
p < 0.001). Both α
2-macroglobulin and tryptase, but not albumin, were increased in BAL fluids from the allergen-challenged side (
p < 0.05).
Conclusion:
Local allergen challenge causes luminal entry of tryptase and α
2-macroglobulin in the nose and bronchi of patients with allergy. We suggest that mast cell and plasma exudation responses may be similar in human nasal and bronchial airways and that albumin levels (in BAL fluids) may not well reflect the exudation process in bronchial airways. (J A
LLERGY C
LIN I
MMUNOL 1995;96:239-46.)</description><identifier>ISSN: 0091-6749</identifier><identifier>EISSN: 1097-6825</identifier><identifier>DOI: 10.1016/S0091-6749(95)70013-7</identifier><identifier>PMID: 7543503</identifier><language>eng</language><publisher>United States: Mosby, Inc</publisher><subject>Adult ; airway inflammation ; albumin ; Allergens - immunology ; Allergic rhinitis ; alpha-Macroglobulins - metabolism ; Bronchi - metabolism ; bronchoalveolar lavage ; Bronchoalveolar Lavage Fluid - chemistry ; Chymases ; Female ; Humans ; Male ; Medicin och hälsovetenskap ; Middle Aged ; Nasal Mucosa - metabolism ; Serine Endopeptidases - metabolism ; tryptase ; Tryptases ; α 2-macroglobulin</subject><ispartof>Journal of allergy and clinical immunology, 1995, Vol.96 (2), p.239-246</ispartof><rights>1995 Mosby, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0091-6749(95)70013-7$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,780,784,885,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7543503$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:1935793$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Svensson, Christer</creatorcontrib><creatorcontrib>Grönneberg, Reidar</creatorcontrib><creatorcontrib>Andersson, Morgan</creatorcontrib><creatorcontrib>Alkner, Ulf</creatorcontrib><creatorcontrib>Andersson, Olof</creatorcontrib><creatorcontrib>Billing, Bo</creatorcontrib><creatorcontrib>Gilljam, Hans</creatorcontrib><creatorcontrib>Greiff, Lennart</creatorcontrib><creatorcontrib>Persson, Carl G.A.</creatorcontrib><title>Allergen challenge-induced entry of α 2-macroglobulin and tryptase into human nasal and bronchial airways</title><title>Journal of allergy and clinical immunology</title><addtitle>J Allergy Clin Immunol</addtitle><description>Background:
Microvascular-epithelial exudation of bulk plasma may characterize inflammatory airway diseases. This study compares the acute allergen challenge–induced mast cell and exudative responses in nasal and bronchial airways. The focus is on α
2-macroglobulin as an index of luminal entry of plasma exudates.
Methods:
Separate nasal and bronchial allergen challenges were carried out outside the pollen season in eight patients with pollen-induced seasonal allergic rhinitis. The levels of different-sized plasma proteins (albumin molecular weight, 66,000 d and α
2-macroglobulin molecular weight, 725,000 d) and tryptase were determined in pre- and postchallenge nasal lavage and bronchoalveolar lavage (BAL) fluids. Diluent and increasing doses of allergen were sprayed into the right nasal cavity, and each challenge was followed by a nasal lavage (volume, 15 ml) with a “nasal pool” device (recovery, >80%). Endobronchial allergen challenge (individual doses) and BAL (volume, 2 × 25 ml) were performed in a lobe bronchus through a fiberoptic bronchoscope (recovery, 30%). Saline challenge and BAL were carried out in the contralateral lung as control.
Results:
The levels of albumin, α
2-macroglobulin, and tryptase increased dose-dependently in postchallenge nasal lavage fluids (
p < 0.05) and correlated to nasal symptoms. In particular, albumin and α
2-macroglobulin correlated (
r = 0.98,
p < 0.001). Both α
2-macroglobulin and tryptase, but not albumin, were increased in BAL fluids from the allergen-challenged side (
p < 0.05).
Conclusion:
Local allergen challenge causes luminal entry of tryptase and α
2-macroglobulin in the nose and bronchi of patients with allergy. We suggest that mast cell and plasma exudation responses may be similar in human nasal and bronchial airways and that albumin levels (in BAL fluids) may not well reflect the exudation process in bronchial airways. (J A
LLERGY C
LIN I
MMUNOL 1995;96:239-46.)</description><subject>Adult</subject><subject>airway inflammation</subject><subject>albumin</subject><subject>Allergens - immunology</subject><subject>Allergic rhinitis</subject><subject>alpha-Macroglobulins - metabolism</subject><subject>Bronchi - metabolism</subject><subject>bronchoalveolar lavage</subject><subject>Bronchoalveolar Lavage Fluid - chemistry</subject><subject>Chymases</subject><subject>Female</subject><subject>Humans</subject><subject>Male</subject><subject>Medicin och hälsovetenskap</subject><subject>Middle Aged</subject><subject>Nasal Mucosa - metabolism</subject><subject>Serine Endopeptidases - metabolism</subject><subject>tryptase</subject><subject>Tryptases</subject><subject>α 2-macroglobulin</subject><issn>0091-6749</issn><issn>1097-6825</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kU1uFDEQhS0ECkPgCJG8QrAw-LfdXqEoIoAUiQWwttx29YxDt3uwuxPNsXIRzoTnh7Bi5ap679klfwhdMPqOUda8_0apYaTR0rwx6q2mlAmin6AVo0aTpuXqKVo9Wp6jF6Xc0tqL1pyhM62kUFSs0O3lMEBeQ8J-42qZ1kBiCouHgCHNeYenHv9-wJyMzudpPUzdMsSEXQq4qtvZFcAxzRPeLKNLOLnihoPa5Sn5Tdx3Md-7XXmJnvVuKPDqdJ6jH9cfv199JjdfP325urwhwIxqCQgPfSOU5rznsnVNb7xkjdCi4x1vfMeDF072PFAVhO6VMMFA6BvpO-WlFOeIHO8t97BdOrvNcXR5ZycX7Wn0s1ZgpZQNb6tf_9e_zVP4F_obZKauZ0RNvj4mq-3XAmW2YywehsElmJZitZaSc86q8eJkXLoRwuMTJwxV_3DUof7LXYRsi4-QKoSYwc82TNEyavfc7YG73UO1RtkDd6vFHyjUooM</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Svensson, Christer</creator><creator>Grönneberg, Reidar</creator><creator>Andersson, Morgan</creator><creator>Alkner, Ulf</creator><creator>Andersson, Olof</creator><creator>Billing, Bo</creator><creator>Gilljam, Hans</creator><creator>Greiff, Lennart</creator><creator>Persson, Carl G.A.</creator><general>Mosby, Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope></search><sort><creationdate>1995</creationdate><title>Allergen challenge-induced entry of α 2-macroglobulin and tryptase into human nasal and bronchial airways</title><author>Svensson, Christer ; Grönneberg, Reidar ; Andersson, Morgan ; Alkner, Ulf ; Andersson, Olof ; Billing, Bo ; Gilljam, Hans ; Greiff, Lennart ; Persson, Carl G.A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e1958-e3cef635722f248a6f9c416373b2b26cb2dc3a4f2d05d37f539d9edf64cb5c443</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Adult</topic><topic>airway inflammation</topic><topic>albumin</topic><topic>Allergens - immunology</topic><topic>Allergic rhinitis</topic><topic>alpha-Macroglobulins - metabolism</topic><topic>Bronchi - metabolism</topic><topic>bronchoalveolar lavage</topic><topic>Bronchoalveolar Lavage Fluid - chemistry</topic><topic>Chymases</topic><topic>Female</topic><topic>Humans</topic><topic>Male</topic><topic>Medicin och hälsovetenskap</topic><topic>Middle Aged</topic><topic>Nasal Mucosa - metabolism</topic><topic>Serine Endopeptidases - metabolism</topic><topic>tryptase</topic><topic>Tryptases</topic><topic>α 2-macroglobulin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Svensson, Christer</creatorcontrib><creatorcontrib>Grönneberg, Reidar</creatorcontrib><creatorcontrib>Andersson, Morgan</creatorcontrib><creatorcontrib>Alkner, Ulf</creatorcontrib><creatorcontrib>Andersson, Olof</creatorcontrib><creatorcontrib>Billing, Bo</creatorcontrib><creatorcontrib>Gilljam, Hans</creatorcontrib><creatorcontrib>Greiff, Lennart</creatorcontrib><creatorcontrib>Persson, Carl G.A.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><jtitle>Journal of allergy and clinical immunology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Svensson, Christer</au><au>Grönneberg, Reidar</au><au>Andersson, Morgan</au><au>Alkner, Ulf</au><au>Andersson, Olof</au><au>Billing, Bo</au><au>Gilljam, Hans</au><au>Greiff, Lennart</au><au>Persson, Carl G.A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Allergen challenge-induced entry of α 2-macroglobulin and tryptase into human nasal and bronchial airways</atitle><jtitle>Journal of allergy and clinical immunology</jtitle><addtitle>J Allergy Clin Immunol</addtitle><date>1995</date><risdate>1995</risdate><volume>96</volume><issue>2</issue><spage>239</spage><epage>246</epage><pages>239-246</pages><issn>0091-6749</issn><eissn>1097-6825</eissn><abstract>Background:
Microvascular-epithelial exudation of bulk plasma may characterize inflammatory airway diseases. This study compares the acute allergen challenge–induced mast cell and exudative responses in nasal and bronchial airways. The focus is on α
2-macroglobulin as an index of luminal entry of plasma exudates.
Methods:
Separate nasal and bronchial allergen challenges were carried out outside the pollen season in eight patients with pollen-induced seasonal allergic rhinitis. The levels of different-sized plasma proteins (albumin molecular weight, 66,000 d and α
2-macroglobulin molecular weight, 725,000 d) and tryptase were determined in pre- and postchallenge nasal lavage and bronchoalveolar lavage (BAL) fluids. Diluent and increasing doses of allergen were sprayed into the right nasal cavity, and each challenge was followed by a nasal lavage (volume, 15 ml) with a “nasal pool” device (recovery, >80%). Endobronchial allergen challenge (individual doses) and BAL (volume, 2 × 25 ml) were performed in a lobe bronchus through a fiberoptic bronchoscope (recovery, 30%). Saline challenge and BAL were carried out in the contralateral lung as control.
Results:
The levels of albumin, α
2-macroglobulin, and tryptase increased dose-dependently in postchallenge nasal lavage fluids (
p < 0.05) and correlated to nasal symptoms. In particular, albumin and α
2-macroglobulin correlated (
r = 0.98,
p < 0.001). Both α
2-macroglobulin and tryptase, but not albumin, were increased in BAL fluids from the allergen-challenged side (
p < 0.05).
Conclusion:
Local allergen challenge causes luminal entry of tryptase and α
2-macroglobulin in the nose and bronchi of patients with allergy. We suggest that mast cell and plasma exudation responses may be similar in human nasal and bronchial airways and that albumin levels (in BAL fluids) may not well reflect the exudation process in bronchial airways. (J A
LLERGY C
LIN I
MMUNOL 1995;96:239-46.)</abstract><cop>United States</cop><pub>Mosby, Inc</pub><pmid>7543503</pmid><doi>10.1016/S0091-6749(95)70013-7</doi><tpages>8</tpages></addata></record> |
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| ispartof | Journal of allergy and clinical immunology, 1995, Vol.96 (2), p.239-246 |
| issn | 0091-6749 1097-6825 |
| language | eng |
| recordid | cdi_swepub_primary_oai_swepub_ki_se_444628 |
| source | MEDLINE; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals |
| subjects | Adult airway inflammation albumin Allergens - immunology Allergic rhinitis alpha-Macroglobulins - metabolism Bronchi - metabolism bronchoalveolar lavage Bronchoalveolar Lavage Fluid - chemistry Chymases Female Humans Male Medicin och hälsovetenskap Middle Aged Nasal Mucosa - metabolism Serine Endopeptidases - metabolism tryptase Tryptases α 2-macroglobulin |
| title | Allergen challenge-induced entry of α 2-macroglobulin and tryptase into human nasal and bronchial airways |
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