Identity of macromolecules present in the extracellular slime layer of Staphylococcus epidermidis
The extracellular slime layer of Staphylococcus epidermidis ATCC 35983 contains: a) two non-anionic carbohydrate containing proteins degradable with papain of molecular masses 250 and 125 kDa; b) a polydisperse but homogeneously-charged acidic population with M r, ranging from 120 000 to 35 000 (ave...
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| Veröffentlicht in: | Biochimie 1995, Vol.77 (3), p.217-224 |
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| container_title | Biochimie |
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| creator | Karamanos, N.K. Panagiotopoulou, H.S. Syrokou, A. Frangides, C. Hjerpe, A. Dimitracopoulos, G. Anastassiou, E.D. |
| description | The extracellular slime layer of
Staphylococcus epidermidis ATCC 35983 contains: a) two non-anionic carbohydrate containing proteins degradable with papain of molecular masses 250 and 125 kDa; b) a polydisperse but homogeneously-charged acidic population with
M
r, ranging from 120 000 to 35 000 (average
M
r 80 000) containing a polysaccharide covalently bound to a small peptide; c) a papain degradable macromolecule (molecular mass 60 kDa) bearing acidic carbohydrates covalently bound to protein; and c) two acidic polysaccharides strongly retained by the anion-exchange column; one polyssacharide is sulphated and has a molecular mass of 20 kDa; the other has a higher charge density and a molecular mass of 12.5 kDa. The results obtained clearly demonstrate the presence of discrete macromolecules in the extracellular material of slime-producing
S epidermidis, the majority of which contain acidic carbohydrates, whose biological role remains to be elucidated. |
| doi_str_mv | 10.1016/0300-9084(96)88127-2 |
| format | Article |
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Staphylococcus epidermidis ATCC 35983 contains: a) two non-anionic carbohydrate containing proteins degradable with papain of molecular masses 250 and 125 kDa; b) a polydisperse but homogeneously-charged acidic population with
M
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M
r 80 000) containing a polysaccharide covalently bound to a small peptide; c) a papain degradable macromolecule (molecular mass 60 kDa) bearing acidic carbohydrates covalently bound to protein; and c) two acidic polysaccharides strongly retained by the anion-exchange column; one polyssacharide is sulphated and has a molecular mass of 20 kDa; the other has a higher charge density and a molecular mass of 12.5 kDa. The results obtained clearly demonstrate the presence of discrete macromolecules in the extracellular material of slime-producing
S epidermidis, the majority of which contain acidic carbohydrates, whose biological role remains to be elucidated.</description><identifier>ISSN: 0300-9084</identifier><identifier>EISSN: 1638-6183</identifier><identifier>DOI: 10.1016/0300-9084(96)88127-2</identifier><identifier>PMID: 7647114</identifier><language>eng</language><publisher>France: Elsevier Masson SAS</publisher><subject>Chemical Fractionation ; Electrophoresis ; extracellular slime layer ; Extracellular Space - chemistry ; glycoconjugates ; Humans ; Staphylococcus epidermidis ; Staphylococcus epidermidis - chemistry ; Staphylococcus epidermidis - ultrastructure</subject><ispartof>Biochimie, 1995, Vol.77 (3), p.217-224</ispartof><rights>1995</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c426t-cde84273a09092528751da6fc2cec117285fab9700d1854c4dc298d6640d3a313</citedby><cites>FETCH-LOGICAL-c426t-cde84273a09092528751da6fc2cec117285fab9700d1854c4dc298d6640d3a313</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0300908496881272$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7647114$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:1940568$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Karamanos, N.K.</creatorcontrib><creatorcontrib>Panagiotopoulou, H.S.</creatorcontrib><creatorcontrib>Syrokou, A.</creatorcontrib><creatorcontrib>Frangides, C.</creatorcontrib><creatorcontrib>Hjerpe, A.</creatorcontrib><creatorcontrib>Dimitracopoulos, G.</creatorcontrib><creatorcontrib>Anastassiou, E.D.</creatorcontrib><title>Identity of macromolecules present in the extracellular slime layer of Staphylococcus epidermidis</title><title>Biochimie</title><addtitle>Biochimie</addtitle><description>The extracellular slime layer of
Staphylococcus epidermidis ATCC 35983 contains: a) two non-anionic carbohydrate containing proteins degradable with papain of molecular masses 250 and 125 kDa; b) a polydisperse but homogeneously-charged acidic population with
M
r, ranging from 120 000 to 35 000 (average
M
r 80 000) containing a polysaccharide covalently bound to a small peptide; c) a papain degradable macromolecule (molecular mass 60 kDa) bearing acidic carbohydrates covalently bound to protein; and c) two acidic polysaccharides strongly retained by the anion-exchange column; one polyssacharide is sulphated and has a molecular mass of 20 kDa; the other has a higher charge density and a molecular mass of 12.5 kDa. The results obtained clearly demonstrate the presence of discrete macromolecules in the extracellular material of slime-producing
S epidermidis, the majority of which contain acidic carbohydrates, whose biological role remains to be elucidated.</description><subject>Chemical Fractionation</subject><subject>Electrophoresis</subject><subject>extracellular slime layer</subject><subject>Extracellular Space - chemistry</subject><subject>glycoconjugates</subject><subject>Humans</subject><subject>Staphylococcus epidermidis</subject><subject>Staphylococcus epidermidis - chemistry</subject><subject>Staphylococcus epidermidis - ultrastructure</subject><issn>0300-9084</issn><issn>1638-6183</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1995</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtP3DAUha2qFQyUf1Akryq6CPgVPzaVKkQpElIX0LXlsW-EW2ec2knb-fckzGiWZXWte75zbd-D0AdKLimh8opwQhpDtLgw8pPWlKmGvUErKrluJNX8LVodkGN0UutPQkhLmDlCR0oKRalYIXcXYDPGcYtzh3vnS-5zAj8lqHgoUGcRxw0enwDDv7E4DylNyRVcU-wBJ7eFslgfRjc8bVP22fupYhhigNLHEOt79K5zqcLZvp6iH19vHq-_Nfffb--uv9w3XjA5Nj6AFkxxRwwxrGVatTQ42XnmwVOqmG47tzaKkEB1K7wInhkdpBQkcMcpP0XNbm79C8O0tkOJvStbm120-9av-QRWCK5bOfMfd_xQ8u8J6mj7WJfvuQ3kqVqlhGw15a-CjHBmpNIzKHbgvMVaC3SHN1Bil8jskodd8rBG2pfILJtt5_v507qHcDDtM5r1zzsd5u39iVBs9RE2HkIs4Ecbcvz_Bc-r6KbU</recordid><startdate>1995</startdate><enddate>1995</enddate><creator>Karamanos, N.K.</creator><creator>Panagiotopoulou, H.S.</creator><creator>Syrokou, A.</creator><creator>Frangides, C.</creator><creator>Hjerpe, A.</creator><creator>Dimitracopoulos, G.</creator><creator>Anastassiou, E.D.</creator><general>Elsevier Masson SAS</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope></search><sort><creationdate>1995</creationdate><title>Identity of macromolecules present in the extracellular slime layer of Staphylococcus epidermidis</title><author>Karamanos, N.K. ; Panagiotopoulou, H.S. ; Syrokou, A. ; Frangides, C. ; Hjerpe, A. ; Dimitracopoulos, G. ; Anastassiou, E.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c426t-cde84273a09092528751da6fc2cec117285fab9700d1854c4dc298d6640d3a313</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1995</creationdate><topic>Chemical Fractionation</topic><topic>Electrophoresis</topic><topic>extracellular slime layer</topic><topic>Extracellular Space - chemistry</topic><topic>glycoconjugates</topic><topic>Humans</topic><topic>Staphylococcus epidermidis</topic><topic>Staphylococcus epidermidis - chemistry</topic><topic>Staphylococcus epidermidis - ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Karamanos, N.K.</creatorcontrib><creatorcontrib>Panagiotopoulou, H.S.</creatorcontrib><creatorcontrib>Syrokou, A.</creatorcontrib><creatorcontrib>Frangides, C.</creatorcontrib><creatorcontrib>Hjerpe, A.</creatorcontrib><creatorcontrib>Dimitracopoulos, G.</creatorcontrib><creatorcontrib>Anastassiou, E.D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><jtitle>Biochimie</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Karamanos, N.K.</au><au>Panagiotopoulou, H.S.</au><au>Syrokou, A.</au><au>Frangides, C.</au><au>Hjerpe, A.</au><au>Dimitracopoulos, G.</au><au>Anastassiou, E.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identity of macromolecules present in the extracellular slime layer of Staphylococcus epidermidis</atitle><jtitle>Biochimie</jtitle><addtitle>Biochimie</addtitle><date>1995</date><risdate>1995</risdate><volume>77</volume><issue>3</issue><spage>217</spage><epage>224</epage><pages>217-224</pages><issn>0300-9084</issn><eissn>1638-6183</eissn><abstract>The extracellular slime layer of
Staphylococcus epidermidis ATCC 35983 contains: a) two non-anionic carbohydrate containing proteins degradable with papain of molecular masses 250 and 125 kDa; b) a polydisperse but homogeneously-charged acidic population with
M
r, ranging from 120 000 to 35 000 (average
M
r 80 000) containing a polysaccharide covalently bound to a small peptide; c) a papain degradable macromolecule (molecular mass 60 kDa) bearing acidic carbohydrates covalently bound to protein; and c) two acidic polysaccharides strongly retained by the anion-exchange column; one polyssacharide is sulphated and has a molecular mass of 20 kDa; the other has a higher charge density and a molecular mass of 12.5 kDa. The results obtained clearly demonstrate the presence of discrete macromolecules in the extracellular material of slime-producing
S epidermidis, the majority of which contain acidic carbohydrates, whose biological role remains to be elucidated.</abstract><cop>France</cop><pub>Elsevier Masson SAS</pub><pmid>7647114</pmid><doi>10.1016/0300-9084(96)88127-2</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0300-9084 |
| ispartof | Biochimie, 1995, Vol.77 (3), p.217-224 |
| issn | 0300-9084 1638-6183 |
| language | eng |
| recordid | cdi_swepub_primary_oai_swepub_ki_se_443856 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Chemical Fractionation Electrophoresis extracellular slime layer Extracellular Space - chemistry glycoconjugates Humans Staphylococcus epidermidis Staphylococcus epidermidis - chemistry Staphylococcus epidermidis - ultrastructure |
| title | Identity of macromolecules present in the extracellular slime layer of Staphylococcus epidermidis |
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