Neutralization of Human Cytomegalovirus by Human CD13-Specific Antibodies
The mechanisms of inhibition of cytomegalovirus (CMV) infection by human CD13 (aminopeptidase N)-specific antibodies were studied. These antibodies protect CD13-negative and -positive cells from CMV infection only if incubated with the virus inoculum, suggesting they bind to CMV virions. The associa...
Gespeichert in:
| Veröffentlicht in: | The Journal of infectious diseases 1996-05, Vol.173 (5), p.1062-1071 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1071 |
|---|---|
| container_issue | 5 |
| container_start_page | 1062 |
| container_title | The Journal of infectious diseases |
| container_volume | 173 |
| creator | Giugni, Terrence D. Söderberg, Cecilia Ham, Debby J. Bautista, Robert M. Hedlund, Kjell-Olof Moller, Erna Zaia, John A. |
| description | The mechanisms of inhibition of cytomegalovirus (CMV) infection by human CD13 (aminopeptidase N)-specific antibodies were studied. These antibodies protect CD13-negative and -positive cells from CMV infection only if incubated with the virus inoculum, suggesting they bind to CMV virions. The association of a CD13-like molecule with virions was further supported by the transfer of CD13 immunoreactivity to the surface of CD13-negative cells upon binding of CMV; the binding of CD13-specific antibodies directly to the surface of CMV virions; and the presence of anti-CD13 immunoreactive bands, including one with mobility similar to that of native cellular CD13 on immunoblots of proteins of purified CMV particles. Importantly, CD13-specific antibodies neutralize CMV in urine of neonates with congenital CMV, indicating that the CD13-like molecule is associated with natural CMV and not acquired in vitro. These studies demonstrate that a CD13-like molecule is associated with CMV particles and may be important in CMV pathogenesis. |
| doi_str_mv | 10.1093/infdis/173.5.1062 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_swepu</sourceid><recordid>TN_cdi_swepub_primary_oai_swepub_ki_se_442028</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>30123004</jstor_id><sourcerecordid>30123004</sourcerecordid><originalsourceid>FETCH-LOGICAL-c655t-fe66d296e655eb784f368e4e5af424225f171224524cd8d78f79dd54703031e03</originalsourceid><addsrcrecordid>eNqFkltv0zAcxSMEGmXwAXhAitDEWzbbf1-SF6Spo-ukcR1IiBfLTezhLomLnWyUT49D025DQjz5cn7nyJeTJM8xOsSogCPbmsqGIyzgkMUdTh4kE8xAZJxjeJhMECIkw3lRPE6ehLBECFHgYi_ZyzkRiLFJcvZO951Xtf2lOuva1Jl03jeqTafrzjX6UtXu2vo-pIv1VjjBkF2sdGmNLdPjtrMLV1kdniaPjKqDfjaO-8mX2ZvP03l2_v70bHp8npWcsS4zmvOKFFzHlV6InBrguaaaKUMJJYQZLDAhlBFaVnklciOKqmJUIECANYL9JNvkhhu96hdy5W2j_Fo6ZeW4dRVnWlJKEMkjL_7Jr7yrbk1bIy4gx3-crzfOKDe6KnU7vNT9gHtKa7_LS3ctBcYI4eGor8YA7370OnSysaHUda1a7fogRT5QIP4LYsZBFAwi-PIvcOl638b3loRAQTChA4Q3UOldCF6b3ZExkkNv5KY3MvZGMjn0Jnpe3L3rzjEWJeoHo65CqWrjVVvGhC0GiEEh4DZmGTrn78iYwFC-3efZ0OmfO135K8kFCCbnX7_JTyeziw_57K38CL8Biunlzg</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>223921243</pqid></control><display><type>article</type><title>Neutralization of Human Cytomegalovirus by Human CD13-Specific Antibodies</title><source>MEDLINE</source><source>SWEPUB Freely available online</source><source>JSTOR Archive Collection A-Z Listing</source><source>Oxford University Press Journals All Titles (1996-Current)</source><creator>Giugni, Terrence D. ; Söderberg, Cecilia ; Ham, Debby J. ; Bautista, Robert M. ; Hedlund, Kjell-Olof ; Moller, Erna ; Zaia, John A.</creator><creatorcontrib>Giugni, Terrence D. ; Söderberg, Cecilia ; Ham, Debby J. ; Bautista, Robert M. ; Hedlund, Kjell-Olof ; Moller, Erna ; Zaia, John A.</creatorcontrib><description>The mechanisms of inhibition of cytomegalovirus (CMV) infection by human CD13 (aminopeptidase N)-specific antibodies were studied. These antibodies protect CD13-negative and -positive cells from CMV infection only if incubated with the virus inoculum, suggesting they bind to CMV virions. The association of a CD13-like molecule with virions was further supported by the transfer of CD13 immunoreactivity to the surface of CD13-negative cells upon binding of CMV; the binding of CD13-specific antibodies directly to the surface of CMV virions; and the presence of anti-CD13 immunoreactive bands, including one with mobility similar to that of native cellular CD13 on immunoblots of proteins of purified CMV particles. Importantly, CD13-specific antibodies neutralize CMV in urine of neonates with congenital CMV, indicating that the CD13-like molecule is associated with natural CMV and not acquired in vitro. These studies demonstrate that a CD13-like molecule is associated with CMV particles and may be important in CMV pathogenesis.</description><identifier>ISSN: 0022-1899</identifier><identifier>EISSN: 1537-6613</identifier><identifier>DOI: 10.1093/infdis/173.5.1062</identifier><identifier>PMID: 8627055</identifier><identifier>CODEN: JIDIAQ</identifier><language>eng</language><publisher>Chicago, IL: The University of Chicago Press</publisher><subject>Animals ; Antibodies ; Antibodies, Monoclonal - immunology ; Antibodies, Viral - immunology ; Antibody Specificity ; Biological and medical sciences ; CD13 Antigens - immunology ; Cell Line ; Cross Reactions ; Cytomegalovirus ; Cytomegalovirus - immunology ; Cytomegalovirus Infections - congenital ; Cytomegalovirus Infections - immunology ; Cytomegalovirus Infections - virology ; Epitopes - analysis ; Female ; Fibroblasts ; human cytomegalovirus ; Human viral diseases ; Humans ; Infant, Newborn ; Infections ; Infectious diseases ; Isotypes ; Major ; Major Articles ; Medical sciences ; Medicin och hälsovetenskap ; Mice ; Miscellaneous ; Molecules ; Monoclonal antibodies ; Neutralization Tests ; NIH 3T3 cells ; Viral diseases ; Virion - metabolism ; Virions ; Viruses</subject><ispartof>The Journal of infectious diseases, 1996-05, Vol.173 (5), p.1062-1071</ispartof><rights>Copyright 1996 The University of Chicago</rights><rights>1996 INIST-CNRS</rights><rights>Copyright University of Chicago, acting through its Press May 1996</rights><rights>1996 by The University of Chicago 1996</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c655t-fe66d296e655eb784f368e4e5af424225f171224524cd8d78f79dd54703031e03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/30123004$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/30123004$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,552,780,784,803,885,27924,27925,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3053973$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8627055$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:1938128$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Giugni, Terrence D.</creatorcontrib><creatorcontrib>Söderberg, Cecilia</creatorcontrib><creatorcontrib>Ham, Debby J.</creatorcontrib><creatorcontrib>Bautista, Robert M.</creatorcontrib><creatorcontrib>Hedlund, Kjell-Olof</creatorcontrib><creatorcontrib>Moller, Erna</creatorcontrib><creatorcontrib>Zaia, John A.</creatorcontrib><title>Neutralization of Human Cytomegalovirus by Human CD13-Specific Antibodies</title><title>The Journal of infectious diseases</title><addtitle>J Infect Dis</addtitle><description>The mechanisms of inhibition of cytomegalovirus (CMV) infection by human CD13 (aminopeptidase N)-specific antibodies were studied. These antibodies protect CD13-negative and -positive cells from CMV infection only if incubated with the virus inoculum, suggesting they bind to CMV virions. The association of a CD13-like molecule with virions was further supported by the transfer of CD13 immunoreactivity to the surface of CD13-negative cells upon binding of CMV; the binding of CD13-specific antibodies directly to the surface of CMV virions; and the presence of anti-CD13 immunoreactive bands, including one with mobility similar to that of native cellular CD13 on immunoblots of proteins of purified CMV particles. Importantly, CD13-specific antibodies neutralize CMV in urine of neonates with congenital CMV, indicating that the CD13-like molecule is associated with natural CMV and not acquired in vitro. These studies demonstrate that a CD13-like molecule is associated with CMV particles and may be important in CMV pathogenesis.</description><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Antibodies, Viral - immunology</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>CD13 Antigens - immunology</subject><subject>Cell Line</subject><subject>Cross Reactions</subject><subject>Cytomegalovirus</subject><subject>Cytomegalovirus - immunology</subject><subject>Cytomegalovirus Infections - congenital</subject><subject>Cytomegalovirus Infections - immunology</subject><subject>Cytomegalovirus Infections - virology</subject><subject>Epitopes - analysis</subject><subject>Female</subject><subject>Fibroblasts</subject><subject>human cytomegalovirus</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infant, Newborn</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Isotypes</subject><subject>Major</subject><subject>Major Articles</subject><subject>Medical sciences</subject><subject>Medicin och hälsovetenskap</subject><subject>Mice</subject><subject>Miscellaneous</subject><subject>Molecules</subject><subject>Monoclonal antibodies</subject><subject>Neutralization Tests</subject><subject>NIH 3T3 cells</subject><subject>Viral diseases</subject><subject>Virion - metabolism</subject><subject>Virions</subject><subject>Viruses</subject><issn>0022-1899</issn><issn>1537-6613</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>D8T</sourceid><recordid>eNqFkltv0zAcxSMEGmXwAXhAitDEWzbbf1-SF6Spo-ukcR1IiBfLTezhLomLnWyUT49D025DQjz5cn7nyJeTJM8xOsSogCPbmsqGIyzgkMUdTh4kE8xAZJxjeJhMECIkw3lRPE6ehLBECFHgYi_ZyzkRiLFJcvZO951Xtf2lOuva1Jl03jeqTafrzjX6UtXu2vo-pIv1VjjBkF2sdGmNLdPjtrMLV1kdniaPjKqDfjaO-8mX2ZvP03l2_v70bHp8npWcsS4zmvOKFFzHlV6InBrguaaaKUMJJYQZLDAhlBFaVnklciOKqmJUIECANYL9JNvkhhu96hdy5W2j_Fo6ZeW4dRVnWlJKEMkjL_7Jr7yrbk1bIy4gx3-crzfOKDe6KnU7vNT9gHtKa7_LS3ctBcYI4eGor8YA7370OnSysaHUda1a7fogRT5QIP4LYsZBFAwi-PIvcOl638b3loRAQTChA4Q3UOldCF6b3ZExkkNv5KY3MvZGMjn0Jnpe3L3rzjEWJeoHo65CqWrjVVvGhC0GiEEh4DZmGTrn78iYwFC-3efZ0OmfO135K8kFCCbnX7_JTyeziw_57K38CL8Biunlzg</recordid><startdate>19960501</startdate><enddate>19960501</enddate><creator>Giugni, Terrence D.</creator><creator>Söderberg, Cecilia</creator><creator>Ham, Debby J.</creator><creator>Bautista, Robert M.</creator><creator>Hedlund, Kjell-Olof</creator><creator>Moller, Erna</creator><creator>Zaia, John A.</creator><general>The University of Chicago Press</general><general>University of Chicago Press</general><general>Oxford University Press</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>NAPCQ</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>ZZAVC</scope></search><sort><creationdate>19960501</creationdate><title>Neutralization of Human Cytomegalovirus by Human CD13-Specific Antibodies</title><author>Giugni, Terrence D. ; Söderberg, Cecilia ; Ham, Debby J. ; Bautista, Robert M. ; Hedlund, Kjell-Olof ; Moller, Erna ; Zaia, John A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c655t-fe66d296e655eb784f368e4e5af424225f171224524cd8d78f79dd54703031e03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Antibodies, Viral - immunology</topic><topic>Antibody Specificity</topic><topic>Biological and medical sciences</topic><topic>CD13 Antigens - immunology</topic><topic>Cell Line</topic><topic>Cross Reactions</topic><topic>Cytomegalovirus</topic><topic>Cytomegalovirus - immunology</topic><topic>Cytomegalovirus Infections - congenital</topic><topic>Cytomegalovirus Infections - immunology</topic><topic>Cytomegalovirus Infections - virology</topic><topic>Epitopes - analysis</topic><topic>Female</topic><topic>Fibroblasts</topic><topic>human cytomegalovirus</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infant, Newborn</topic><topic>Infections</topic><topic>Infectious diseases</topic><topic>Isotypes</topic><topic>Major</topic><topic>Major Articles</topic><topic>Medical sciences</topic><topic>Medicin och hälsovetenskap</topic><topic>Mice</topic><topic>Miscellaneous</topic><topic>Molecules</topic><topic>Monoclonal antibodies</topic><topic>Neutralization Tests</topic><topic>NIH 3T3 cells</topic><topic>Viral diseases</topic><topic>Virion - metabolism</topic><topic>Virions</topic><topic>Viruses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Giugni, Terrence D.</creatorcontrib><creatorcontrib>Söderberg, Cecilia</creatorcontrib><creatorcontrib>Ham, Debby J.</creatorcontrib><creatorcontrib>Bautista, Robert M.</creatorcontrib><creatorcontrib>Hedlund, Kjell-Olof</creatorcontrib><creatorcontrib>Moller, Erna</creatorcontrib><creatorcontrib>Zaia, John A.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Premium</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SwePub Articles full text</collection><jtitle>The Journal of infectious diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Giugni, Terrence D.</au><au>Söderberg, Cecilia</au><au>Ham, Debby J.</au><au>Bautista, Robert M.</au><au>Hedlund, Kjell-Olof</au><au>Moller, Erna</au><au>Zaia, John A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Neutralization of Human Cytomegalovirus by Human CD13-Specific Antibodies</atitle><jtitle>The Journal of infectious diseases</jtitle><addtitle>J Infect Dis</addtitle><date>1996-05-01</date><risdate>1996</risdate><volume>173</volume><issue>5</issue><spage>1062</spage><epage>1071</epage><pages>1062-1071</pages><issn>0022-1899</issn><eissn>1537-6613</eissn><coden>JIDIAQ</coden><abstract>The mechanisms of inhibition of cytomegalovirus (CMV) infection by human CD13 (aminopeptidase N)-specific antibodies were studied. These antibodies protect CD13-negative and -positive cells from CMV infection only if incubated with the virus inoculum, suggesting they bind to CMV virions. The association of a CD13-like molecule with virions was further supported by the transfer of CD13 immunoreactivity to the surface of CD13-negative cells upon binding of CMV; the binding of CD13-specific antibodies directly to the surface of CMV virions; and the presence of anti-CD13 immunoreactive bands, including one with mobility similar to that of native cellular CD13 on immunoblots of proteins of purified CMV particles. Importantly, CD13-specific antibodies neutralize CMV in urine of neonates with congenital CMV, indicating that the CD13-like molecule is associated with natural CMV and not acquired in vitro. These studies demonstrate that a CD13-like molecule is associated with CMV particles and may be important in CMV pathogenesis.</abstract><cop>Chicago, IL</cop><pub>The University of Chicago Press</pub><pmid>8627055</pmid><doi>10.1093/infdis/173.5.1062</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1899 |
| ispartof | The Journal of infectious diseases, 1996-05, Vol.173 (5), p.1062-1071 |
| issn | 0022-1899 1537-6613 |
| language | eng |
| recordid | cdi_swepub_primary_oai_swepub_ki_se_442028 |
| source | MEDLINE; SWEPUB Freely available online; JSTOR Archive Collection A-Z Listing; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Animals Antibodies Antibodies, Monoclonal - immunology Antibodies, Viral - immunology Antibody Specificity Biological and medical sciences CD13 Antigens - immunology Cell Line Cross Reactions Cytomegalovirus Cytomegalovirus - immunology Cytomegalovirus Infections - congenital Cytomegalovirus Infections - immunology Cytomegalovirus Infections - virology Epitopes - analysis Female Fibroblasts human cytomegalovirus Human viral diseases Humans Infant, Newborn Infections Infectious diseases Isotypes Major Major Articles Medical sciences Medicin och hälsovetenskap Mice Miscellaneous Molecules Monoclonal antibodies Neutralization Tests NIH 3T3 cells Viral diseases Virion - metabolism Virions Viruses |
| title | Neutralization of Human Cytomegalovirus by Human CD13-Specific Antibodies |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-24T12%3A39%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_swepu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Neutralization%20of%20Human%20Cytomegalovirus%20by%20Human%20CD13-Specific%20Antibodies&rft.jtitle=The%20Journal%20of%20infectious%20diseases&rft.au=Giugni,%20Terrence%20D.&rft.date=1996-05-01&rft.volume=173&rft.issue=5&rft.spage=1062&rft.epage=1071&rft.pages=1062-1071&rft.issn=0022-1899&rft.eissn=1537-6613&rft.coden=JIDIAQ&rft_id=info:doi/10.1093/infdis/173.5.1062&rft_dat=%3Cjstor_swepu%3E30123004%3C/jstor_swepu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=223921243&rft_id=info:pmid/8627055&rft_jstor_id=30123004&rfr_iscdi=true |