Involvement of Cellular Proteolytic Machinery in Apoptosis

Programmed cell death (PCD), a genetically controlled cell deletion process, plays an important role in the regulation of cellular and tissue homeostasis. The requisite for proteolysis during PCD-induced apoptosis is well documented. The cellular proteolytic machinery includes numerous proteases localized in membranes, cytoplasm, and nucleus. This machinery may function to remove denatured or misfolded protein from the cytoplasm on a routine basis and may also cleave proteins thereby implementing their activation. The well established role of some proteases is to maintain fundamental cellular processes; however, the precise cellular location and function of other proteases which make a contribution to a unique unidirectional process such as apoptosis remains unclear. The functional overlap between ‘scheduled’ and ‘unscheduled’ proteolysis may potentially lead to confusion in this research area. In this review we will discuss certain cellular proteolytic systems and highlight the possible involvement of each in apoptosis.