Adhesion properties of human alveolar macrophages with respect to extracellular matrix components and chemotactic agonists
Adherence has an essential impact on the differentiation and activation of tissue dwelling monocytes/macrophages. We have considered the effect of selected chemotactic agonists (fMLP, RANTES, IL-8) on the adhesion properties of human alveolar macrophages prepared by bronchoalveolar lavage. The macro...
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| Veröffentlicht in: | Immunology letters 1996-11, Vol.53 (2), p.121-124 |
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| container_title | Immunology letters |
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| creator | Lundahl, J. Eklund, A. Halldén, G. Sköld, C.M. |
| description | Adherence has an essential impact on the differentiation and activation of tissue dwelling monocytes/macrophages. We have considered the effect of selected chemotactic agonists (fMLP, RANTES, IL-8) on the adhesion properties of human alveolar macrophages prepared by bronchoalveolar lavage. The macrophages were co-incubated in buffer alone or buffer supplemented with respective agonist, for different time points, in culture wells precoated with albumin, vitronectin and fibronectin, respectively. The macrophages displayed a gradual increase in adhesion to all three surfaces and discriminated between the different matrix components, but did not respond to the selected agonists with increased adhesion. |
| doi_str_mv | 10.1016/S0165-2478(96)02621-1 |
| format | Article |
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We have considered the effect of selected chemotactic agonists (fMLP, RANTES, IL-8) on the adhesion properties of human alveolar macrophages prepared by bronchoalveolar lavage. The macrophages were co-incubated in buffer alone or buffer supplemented with respective agonist, for different time points, in culture wells precoated with albumin, vitronectin and fibronectin, respectively. The macrophages displayed a gradual increase in adhesion to all three surfaces and discriminated between the different matrix components, but did not respond to the selected agonists with increased adhesion.</description><subject>Adhesion</subject><subject>Adult</subject><subject>Albumins - metabolism</subject><subject>Alveolar macrophages</subject><subject>Bronchoalveolar Lavage Fluid - cytology</subject><subject>Cell Adhesion - drug effects</subject><subject>Chemokine CCL5 - pharmacology</subject><subject>Chemokines</subject><subject>Fibronectins - metabolism</subject><subject>Flow Cytometry</subject><subject>Humans</subject><subject>Interleukin-8 - pharmacology</subject><subject>Macrophage Activation</subject><subject>Macrophages, Alveolar - drug effects</subject><subject>Macrophages, Alveolar - physiology</subject><subject>Matrix</subject><subject>Medicin och hälsovetenskap</subject><subject>N-Formylmethionine Leucyl-Phenylalanine - pharmacology</subject><subject>Protein Binding</subject><subject>Time Factors</subject><subject>Vitronectin - metabolism</subject><issn>0165-2478</issn><issn>1879-0542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtv1DAUhS0EKsPAT6jkFYJFwI_EsVeoqnhJlVgAa8t2bhpDEgfbaQu_Hk8zDCvExrZ8zrlXOh9C55S8ooSK15_L0VSsbuULJV4SJhit6AO0o7JVFWlq9hDtTpbH6ElK3wihDa_5GTpThNVKqh36ddENkHyY8RLDAjF7SDj0eFgnM2Mz3kAYTcSTcUUezHVRb30ecIS0gMs4Bwx3ORoH47huzhz9HXZhWsIMc07YzB12A0whG5e9w-Y6zD7l9BQ96s2Y4Nnx3qOv795-ufxQXX16__Hy4qpydS1yxSmTDRXcWdcqa5R1Stimk6aXTnSSW9X21tasVdJxUivWKSc5MMppQ7hlfI-qbW66hWW1eol-MvGnDsbr49f38gJdc0UoL_72n_5SUvc39CdIFedCkZJ8viWL7ccKKevJp0MzZoawJt1K0baqENijZjOWVlOK0J-WUKIPdPU9XX1Ap5XQ93Q1Lbnz44LVTtCdUkecRX-z6VD6vPEQdXIeZgedj4WW7oL_z4bfSbW5CQ</recordid><startdate>19961101</startdate><enddate>19961101</enddate><creator>Lundahl, J.</creator><creator>Eklund, A.</creator><creator>Halldén, G.</creator><creator>Sköld, C.M.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope></search><sort><creationdate>19961101</creationdate><title>Adhesion properties of human alveolar macrophages with respect to extracellular matrix components and chemotactic agonists</title><author>Lundahl, J. ; Eklund, A. ; Halldén, G. ; Sköld, C.M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c446t-31285163cbc79ba9bc96b5d8af8c6d83b97fbb42798c30492d9c83e2131503b23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Adhesion</topic><topic>Adult</topic><topic>Albumins - metabolism</topic><topic>Alveolar macrophages</topic><topic>Bronchoalveolar Lavage Fluid - cytology</topic><topic>Cell Adhesion - drug effects</topic><topic>Chemokine CCL5 - pharmacology</topic><topic>Chemokines</topic><topic>Fibronectins - metabolism</topic><topic>Flow Cytometry</topic><topic>Humans</topic><topic>Interleukin-8 - pharmacology</topic><topic>Macrophage Activation</topic><topic>Macrophages, Alveolar - drug effects</topic><topic>Macrophages, Alveolar - physiology</topic><topic>Matrix</topic><topic>Medicin och hälsovetenskap</topic><topic>N-Formylmethionine Leucyl-Phenylalanine - pharmacology</topic><topic>Protein Binding</topic><topic>Time Factors</topic><topic>Vitronectin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lundahl, J.</creatorcontrib><creatorcontrib>Eklund, A.</creatorcontrib><creatorcontrib>Halldén, G.</creatorcontrib><creatorcontrib>Sköld, C.M.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><jtitle>Immunology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lundahl, J.</au><au>Eklund, A.</au><au>Halldén, G.</au><au>Sköld, C.M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Adhesion properties of human alveolar macrophages with respect to extracellular matrix components and chemotactic agonists</atitle><jtitle>Immunology letters</jtitle><addtitle>Immunol Lett</addtitle><date>1996-11-01</date><risdate>1996</risdate><volume>53</volume><issue>2</issue><spage>121</spage><epage>124</epage><pages>121-124</pages><issn>0165-2478</issn><eissn>1879-0542</eissn><abstract>Adherence has an essential impact on the differentiation and activation of tissue dwelling monocytes/macrophages. 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| fulltext | fulltext |
| identifier | ISSN: 0165-2478 |
| ispartof | Immunology letters, 1996-11, Vol.53 (2), p.121-124 |
| issn | 0165-2478 1879-0542 |
| language | eng |
| recordid | cdi_swepub_primary_oai_swepub_ki_se_439013 |
| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Adhesion Adult Albumins - metabolism Alveolar macrophages Bronchoalveolar Lavage Fluid - cytology Cell Adhesion - drug effects Chemokine CCL5 - pharmacology Chemokines Fibronectins - metabolism Flow Cytometry Humans Interleukin-8 - pharmacology Macrophage Activation Macrophages, Alveolar - drug effects Macrophages, Alveolar - physiology Matrix Medicin och hälsovetenskap N-Formylmethionine Leucyl-Phenylalanine - pharmacology Protein Binding Time Factors Vitronectin - metabolism |
| title | Adhesion properties of human alveolar macrophages with respect to extracellular matrix components and chemotactic agonists |
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