Species Differences in Cardiac Thyroid Hormone Receptor Isoforms Protein Abundance
Little is known about the cardiac expression of different thyroid hormone receptor (TR) isoforms. The aim of the study was to investigate such patterns of TR expression at the protein level in different species and in some human tissues. Western blot analysis with specific polyclonal rabbit antibodi...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 1997/11/15, Vol.20(11), pp.1123-1126 |
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| creator | BLANGE, Irina DRVOTA, Victor YEN, Paul M. SYLVEN, Chister |
| description | Little is known about the cardiac expression of different thyroid hormone receptor (TR) isoforms. The aim of the study was to investigate such patterns of TR expression at the protein level in different species and in some human tissues. Western blot analysis with specific polyclonal rabbit antibodies to each TR isoform was performed with samples from myocardium of the left ventricle from man, dog, guinea pig, rat and mouse, as well as with samples from several human tissues such as heart, skeletal muscle, brain, liver and thyroid. The TRα1 isoform was present in all of the species examined. The TRα2 was recognized in human, dog and guinea pig heart, while no such band was recognized in rat and mouse hearts. TRβ1 was not detected in the human heart but in the other species. Simularly to TRα1, TRβ2 was detected in all of the species examined. In the human tissues studied, TRα1 was detected in heart and skeletal muscle, whereas TRα2 was found only in the heart. TRβ1 was not detected in any of the examined human tissues, while TRβ2 was found in all of them. These results revealed unique distributions of TR variants and they demonstrate common epitopes in TR in the different species. For the first time, the presence of a TRβ2 isoform has been shown in human tissues. TR isoforms may have a tissue and species specific role in the regulation of gene expression and may in part explain variable tissue effects of thyroid hormones. |
| doi_str_mv | 10.1248/bpb.20.1123 |
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The aim of the study was to investigate such patterns of TR expression at the protein level in different species and in some human tissues. Western blot analysis with specific polyclonal rabbit antibodies to each TR isoform was performed with samples from myocardium of the left ventricle from man, dog, guinea pig, rat and mouse, as well as with samples from several human tissues such as heart, skeletal muscle, brain, liver and thyroid. The TRα1 isoform was present in all of the species examined. The TRα2 was recognized in human, dog and guinea pig heart, while no such band was recognized in rat and mouse hearts. TRβ1 was not detected in the human heart but in the other species. Simularly to TRα1, TRβ2 was detected in all of the species examined. In the human tissues studied, TRα1 was detected in heart and skeletal muscle, whereas TRα2 was found only in the heart. TRβ1 was not detected in any of the examined human tissues, while TRβ2 was found in all of them. These results revealed unique distributions of TR variants and they demonstrate common epitopes in TR in the different species. For the first time, the presence of a TRβ2 isoform has been shown in human tissues. TR isoforms may have a tissue and species specific role in the regulation of gene expression and may in part explain variable tissue effects of thyroid hormones.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.20.1123</identifier><identifier>PMID: 9401716</identifier><language>eng</language><publisher>Tokyo: The Pharmaceutical Society of Japan</publisher><subject>Animals ; Antibody Specificity ; Biological and medical sciences ; Blotting, Western ; Brain - metabolism ; Cell receptors ; Cell structures and functions ; dog ; Dogs ; Fundamental and applied biological sciences. Psychology ; guinea pig ; Guinea Pigs ; Heart Ventricles - metabolism ; Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors ; human ; Humans ; Liver - metabolism ; Medicin och hälsovetenskap ; Mice ; Mice, Inbred Strains ; Molecular and cellular biology ; mouse ; Muscle, Skeletal - metabolism ; rat ; Rats ; Rats, Sprague-Dawley ; Receptors, Thyroid Hormone - immunology ; Receptors, Thyroid Hormone - metabolism ; Species Specificity ; Thyroid Gland - metabolism ; thyroid hormone receptor</subject><ispartof>Biological and Pharmaceutical Bulletin, 1997/11/15, Vol.20(11), pp.1123-1126</ispartof><rights>The Pharmaceutical Society of Japan</rights><rights>1998 INIST-CNRS</rights><rights>Copyright Japan Science and Technology Agency 1997</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c719t-de7b3ced8ab781bfdb7a76900a6b9ee6e35dd089064a6654ad54da0d76a106b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,1883,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=2104397$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9401716$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:1944595$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>BLANGE, Irina</creatorcontrib><creatorcontrib>DRVOTA, Victor</creatorcontrib><creatorcontrib>YEN, Paul M.</creatorcontrib><creatorcontrib>SYLVEN, Chister</creatorcontrib><title>Species Differences in Cardiac Thyroid Hormone Receptor Isoforms Protein Abundance</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>Little is known about the cardiac expression of different thyroid hormone receptor (TR) isoforms. The aim of the study was to investigate such patterns of TR expression at the protein level in different species and in some human tissues. Western blot analysis with specific polyclonal rabbit antibodies to each TR isoform was performed with samples from myocardium of the left ventricle from man, dog, guinea pig, rat and mouse, as well as with samples from several human tissues such as heart, skeletal muscle, brain, liver and thyroid. The TRα1 isoform was present in all of the species examined. The TRα2 was recognized in human, dog and guinea pig heart, while no such band was recognized in rat and mouse hearts. TRβ1 was not detected in the human heart but in the other species. Simularly to TRα1, TRβ2 was detected in all of the species examined. In the human tissues studied, TRα1 was detected in heart and skeletal muscle, whereas TRα2 was found only in the heart. TRβ1 was not detected in any of the examined human tissues, while TRβ2 was found in all of them. These results revealed unique distributions of TR variants and they demonstrate common epitopes in TR in the different species. For the first time, the presence of a TRβ2 isoform has been shown in human tissues. TR isoforms may have a tissue and species specific role in the regulation of gene expression and may in part explain variable tissue effects of thyroid hormones.</description><subject>Animals</subject><subject>Antibody Specificity</subject><subject>Biological and medical sciences</subject><subject>Blotting, Western</subject><subject>Brain - metabolism</subject><subject>Cell receptors</subject><subject>Cell structures and functions</subject><subject>dog</subject><subject>Dogs</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>guinea pig</subject><subject>Guinea Pigs</subject><subject>Heart Ventricles - metabolism</subject><subject>Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors</subject><subject>human</subject><subject>Humans</subject><subject>Liver - metabolism</subject><subject>Medicin och hälsovetenskap</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Molecular and cellular biology</subject><subject>mouse</subject><subject>Muscle, Skeletal - metabolism</subject><subject>rat</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Receptors, Thyroid Hormone - immunology</subject><subject>Receptors, Thyroid Hormone - metabolism</subject><subject>Species Specificity</subject><subject>Thyroid Gland - metabolism</subject><subject>thyroid hormone receptor</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1997</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kd9r1TAUx4M45nX65LNQcOxFOpMmTZrHcd3cYKDM-x7y49Tl2tvUpEX235tLawVhL8mXcz7fc0K-CL0j-JJUrPlkBnNZZU0q-gJtCGWirCtSv0QbLElTclI3r9DrlPYYY4EreopOJcNEEL5BD98HsB5S8dm3LUTobda-L7Y6Oq9tsXt8isG74jbEQ-iheAALwxhicZdCm2up-BbDCNlxZabe6ex_g05a3SV4u9xnaHdzvdvelvdfv9xtr-5LK4gcSwfCUAuu0UY0xLTOCC24xFhzIwE40No53EjMmea8ZtrVzGnsBNcEc0PPUDmPTb9hmIwaoj_o-KSC9mop_cwKFKO8Yjjz4ll-iMH9M_01EslYLevsvJidGfs1QRrVwScLXad7CFNSQjIum1pk8MN_4D5Msc-foAhjknDJJcnUx5myMaQUoV2fQrA6BqpyoKrKOgea6ffLzMkcwK3skmDuny99nazu2pgj8GnFKoIZlcen3czYPo36B6x9HUdvOziuJFLSee1yHvevgH3UUUFP_wCRHcII</recordid><startdate>1997</startdate><enddate>1997</enddate><creator>BLANGE, Irina</creator><creator>DRVOTA, Victor</creator><creator>YEN, Paul M.</creator><creator>SYLVEN, Chister</creator><general>The Pharmaceutical Society of Japan</general><general>Maruzen</general><general>Japan Science and Technology Agency</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope></search><sort><creationdate>1997</creationdate><title>Species Differences in Cardiac Thyroid Hormone Receptor Isoforms Protein Abundance</title><author>BLANGE, Irina ; DRVOTA, Victor ; YEN, Paul M. ; SYLVEN, Chister</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c719t-de7b3ced8ab781bfdb7a76900a6b9ee6e35dd089064a6654ad54da0d76a106b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1997</creationdate><topic>Animals</topic><topic>Antibody Specificity</topic><topic>Biological and medical sciences</topic><topic>Blotting, Western</topic><topic>Brain - metabolism</topic><topic>Cell receptors</topic><topic>Cell structures and functions</topic><topic>dog</topic><topic>Dogs</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>guinea pig</topic><topic>Guinea Pigs</topic><topic>Heart Ventricles - metabolism</topic><topic>Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors</topic><topic>human</topic><topic>Humans</topic><topic>Liver - metabolism</topic><topic>Medicin och hälsovetenskap</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Molecular and cellular biology</topic><topic>mouse</topic><topic>Muscle, Skeletal - metabolism</topic><topic>rat</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Receptors, Thyroid Hormone - immunology</topic><topic>Receptors, Thyroid Hormone - metabolism</topic><topic>Species Specificity</topic><topic>Thyroid Gland - metabolism</topic><topic>thyroid hormone receptor</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>BLANGE, Irina</creatorcontrib><creatorcontrib>DRVOTA, Victor</creatorcontrib><creatorcontrib>YEN, Paul M.</creatorcontrib><creatorcontrib>SYLVEN, Chister</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>BLANGE, Irina</au><au>DRVOTA, Victor</au><au>YEN, Paul M.</au><au>SYLVEN, Chister</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Species Differences in Cardiac Thyroid Hormone Receptor Isoforms Protein Abundance</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>1997</date><risdate>1997</risdate><volume>20</volume><issue>11</issue><spage>1123</spage><epage>1126</epage><pages>1123-1126</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>Little is known about the cardiac expression of different thyroid hormone receptor (TR) isoforms. The aim of the study was to investigate such patterns of TR expression at the protein level in different species and in some human tissues. Western blot analysis with specific polyclonal rabbit antibodies to each TR isoform was performed with samples from myocardium of the left ventricle from man, dog, guinea pig, rat and mouse, as well as with samples from several human tissues such as heart, skeletal muscle, brain, liver and thyroid. The TRα1 isoform was present in all of the species examined. The TRα2 was recognized in human, dog and guinea pig heart, while no such band was recognized in rat and mouse hearts. TRβ1 was not detected in the human heart but in the other species. Simularly to TRα1, TRβ2 was detected in all of the species examined. In the human tissues studied, TRα1 was detected in heart and skeletal muscle, whereas TRα2 was found only in the heart. TRβ1 was not detected in any of the examined human tissues, while TRβ2 was found in all of them. These results revealed unique distributions of TR variants and they demonstrate common epitopes in TR in the different species. For the first time, the presence of a TRβ2 isoform has been shown in human tissues. TR isoforms may have a tissue and species specific role in the regulation of gene expression and may in part explain variable tissue effects of thyroid hormones.</abstract><cop>Tokyo</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>9401716</pmid><doi>10.1248/bpb.20.1123</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Biological and Pharmaceutical Bulletin, 1997/11/15, Vol.20(11), pp.1123-1126 |
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| source | MEDLINE; J-STAGE (Japan Science & Technology Information Aggregator, Electronic) Freely Available Titles - Japanese; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Animals Antibody Specificity Biological and medical sciences Blotting, Western Brain - metabolism Cell receptors Cell structures and functions dog Dogs Fundamental and applied biological sciences. Psychology guinea pig Guinea Pigs Heart Ventricles - metabolism Hormone receptors. Growth factor receptors. Cytokine receptors. Prostaglandin receptors human Humans Liver - metabolism Medicin och hälsovetenskap Mice Mice, Inbred Strains Molecular and cellular biology mouse Muscle, Skeletal - metabolism rat Rats Rats, Sprague-Dawley Receptors, Thyroid Hormone - immunology Receptors, Thyroid Hormone - metabolism Species Specificity Thyroid Gland - metabolism thyroid hormone receptor |
| title | Species Differences in Cardiac Thyroid Hormone Receptor Isoforms Protein Abundance |
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