Identification of New Mediator Subunits in the RNA Polymerase II Holoenzyme from Saccharomyces cerevisiae
Mediator was isolated from yeast on the basis of its requirement for transcriptional activation in a fully defined system. We have now identified three new members of mediator in the low molecular mass range by peptide sequence determination. These are the products of the NUT2, CSE2, and MED11 genes...
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| Veröffentlicht in: | The Journal of biological chemistry 1998-11, Vol.273 (47), p.30851-30854 |
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| container_issue | 47 |
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| container_title | The Journal of biological chemistry |
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| creator | Gustafsson, Claes M. Myers, Lawrence C. Beve, Jenny Spåhr, Henrik Lui, Mary Erdjument-Bromage, Hediye Tempst, Paul Kornberg, Roger D. |
| description | Mediator was isolated from yeast on the basis of its requirement for transcriptional activation in a fully defined system. We have now identified three new members of mediator in the low molecular mass range by peptide sequence determination. These are the products of the NUT2, CSE2, and MED11 genes. The product of the NUT1 gene is evidently a component of mediator as well. NUT1 and NUT2 were earlier identified as negative regulators of the HO promoter, whereas mutations in CSE2 affect chromosome segregation.MED11 is a previously uncharacterized gene. The existence of these proteins in the mediator complex was verified by copurification and co-immunoprecipitation with RNA polymerase II holoenzyme. |
| doi_str_mv | 10.1074/jbc.273.47.30851 |
| format | Article |
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We have now identified three new members of mediator in the low molecular mass range by peptide sequence determination. These are the products of the NUT2, CSE2, and MED11 genes. The product of the NUT1 gene is evidently a component of mediator as well. NUT1 and NUT2 were earlier identified as negative regulators of the HO promoter, whereas mutations in CSE2 affect chromosome segregation.MED11 is a previously uncharacterized gene. The existence of these proteins in the mediator complex was verified by copurification and co-immunoprecipitation with RNA polymerase II holoenzyme.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.273.47.30851</identifier><identifier>PMID: 9812975</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Amino Acid Sequence ; AMINO ACID SEQUENCES ; BINDING PROTEINS ; CHEMICAL COMPOSITION ; COMPOSICION QUIMICA ; COMPOSITION CHIMIQUE ; Fungal Proteins - analysis ; Fungal Proteins - genetics ; Holoenzymes - chemistry ; Mediator Complex ; Medicin och hälsovetenskap ; MOLECULAR SEQUENCE DATA ; MOLECULAR WEIGHT ; Nuclear Proteins - analysis ; Nuclear Proteins - genetics ; PESO MOLECULAR ; POIDS MOLECULAIRE ; Precipitin Tests ; Protein Binding ; PROTEINAS AGLUTINANTES ; PROTEINE DE LIAISON ; PURIFICACION ; PURIFICATION ; RNA Polymerase II - chemistry ; SACCHAROMYCES CEREVISIAE ; Saccharomyces cerevisiae - enzymology ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae Proteins ; Sequence Analysis ; Sequence Homology, Amino Acid ; Transcription Factors ; TRANSFERASAS ; TRANSFERASE ; TRANSFERASES</subject><ispartof>The Journal of biological chemistry, 1998-11, Vol.273 (47), p.30851-30854</ispartof><rights>1998 © 1998 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c556t-f8de4334ad67da412fb2a2ec6f65f76d670bbf1882d881a29ef684ba669b738f3</citedby><cites>FETCH-LOGICAL-c556t-f8de4334ad67da412fb2a2ec6f65f76d670bbf1882d881a29ef684ba669b738f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,552,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/9812975$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:15329990$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Gustafsson, Claes M.</creatorcontrib><creatorcontrib>Myers, Lawrence C.</creatorcontrib><creatorcontrib>Beve, Jenny</creatorcontrib><creatorcontrib>Spåhr, Henrik</creatorcontrib><creatorcontrib>Lui, Mary</creatorcontrib><creatorcontrib>Erdjument-Bromage, Hediye</creatorcontrib><creatorcontrib>Tempst, Paul</creatorcontrib><creatorcontrib>Kornberg, Roger D.</creatorcontrib><title>Identification of New Mediator Subunits in the RNA Polymerase II Holoenzyme from Saccharomyces cerevisiae</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Mediator was isolated from yeast on the basis of its requirement for transcriptional activation in a fully defined system. We have now identified three new members of mediator in the low molecular mass range by peptide sequence determination. These are the products of the NUT2, CSE2, and MED11 genes. The product of the NUT1 gene is evidently a component of mediator as well. NUT1 and NUT2 were earlier identified as negative regulators of the HO promoter, whereas mutations in CSE2 affect chromosome segregation.MED11 is a previously uncharacterized gene. The existence of these proteins in the mediator complex was verified by copurification and co-immunoprecipitation with RNA polymerase II holoenzyme.</description><subject>Amino Acid Sequence</subject><subject>AMINO ACID SEQUENCES</subject><subject>BINDING PROTEINS</subject><subject>CHEMICAL COMPOSITION</subject><subject>COMPOSICION QUIMICA</subject><subject>COMPOSITION CHIMIQUE</subject><subject>Fungal Proteins - analysis</subject><subject>Fungal Proteins - genetics</subject><subject>Holoenzymes - chemistry</subject><subject>Mediator Complex</subject><subject>Medicin och hälsovetenskap</subject><subject>MOLECULAR SEQUENCE DATA</subject><subject>MOLECULAR WEIGHT</subject><subject>Nuclear Proteins - analysis</subject><subject>Nuclear Proteins - genetics</subject><subject>PESO MOLECULAR</subject><subject>POIDS MOLECULAIRE</subject><subject>Precipitin Tests</subject><subject>Protein Binding</subject><subject>PROTEINAS AGLUTINANTES</subject><subject>PROTEINE DE LIAISON</subject><subject>PURIFICACION</subject><subject>PURIFICATION</subject><subject>RNA Polymerase II - chemistry</subject><subject>SACCHAROMYCES CEREVISIAE</subject><subject>Saccharomyces cerevisiae - enzymology</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae Proteins</subject><subject>Sequence Analysis</subject><subject>Sequence Homology, Amino Acid</subject><subject>Transcription Factors</subject><subject>TRANSFERASAS</subject><subject>TRANSFERASE</subject><subject>TRANSFERASES</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1998</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>D8T</sourceid><recordid>eNqFUs9vFCEYJUZTt9W7FxMOxtuswPwAvDWNtpvUalybeCMM89GhzgwrzHSz_vWyzlpPG-HAl_e99_jCA6FXlCwp4cW7-9osGc-XBV_mRJT0CVpQIvIsL-n3p2hBCKOZZKV4jk5jvCdpFZKeoBMpKJO8XCC3amAYnXVGj84P2Ft8A1v8CRqnRx_weqqnwY0RuwGPLeCvN-f4i-92PQQdAa9W-Mp3HoZfCcE2-B6vtTGtTtXOQMQGAjy46DS8QM-s7iK8PJxn6Pbjh28XV9n158vVxfl1ZsqyGjMrGijyvNBNxRtdUGZrphmYylal5VVCSV1bKgRrhKCaSbCVKGpdVbLmubD5Gcpm37iFzVSrTXC9DjvltVMH6EeqQKVbGCkSXxzlb4Jv_on-CmmZMyklSdK3szTxfk4QR9W7aKDr9AB-iorvnzvt_xIpp7Tk1Z5IZqIJPsYA9nEcStQ-cZUSVylxVXD1J_EkeX3wnuoemkfBIeLUfzP3W3fXbl0AVTtvWuiP2Fjtlb4LLqrbNZWSE1kIsrd5P_chZffgIKhoHAwmfZQAZlSNd8dn_A159tGG</recordid><startdate>19981120</startdate><enddate>19981120</enddate><creator>Gustafsson, Claes M.</creator><creator>Myers, Lawrence C.</creator><creator>Beve, Jenny</creator><creator>Spåhr, Henrik</creator><creator>Lui, Mary</creator><creator>Erdjument-Bromage, Hediye</creator><creator>Tempst, Paul</creator><creator>Kornberg, Roger D.</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>M7N</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>ZZAVC</scope></search><sort><creationdate>19981120</creationdate><title>Identification of New Mediator Subunits in the RNA Polymerase II Holoenzyme from Saccharomyces cerevisiae</title><author>Gustafsson, Claes M. ; Myers, Lawrence C. ; Beve, Jenny ; Spåhr, Henrik ; Lui, Mary ; Erdjument-Bromage, Hediye ; Tempst, Paul ; Kornberg, Roger D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c556t-f8de4334ad67da412fb2a2ec6f65f76d670bbf1882d881a29ef684ba669b738f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1998</creationdate><topic>Amino Acid Sequence</topic><topic>AMINO ACID SEQUENCES</topic><topic>BINDING PROTEINS</topic><topic>CHEMICAL COMPOSITION</topic><topic>COMPOSICION QUIMICA</topic><topic>COMPOSITION CHIMIQUE</topic><topic>Fungal Proteins - analysis</topic><topic>Fungal Proteins - genetics</topic><topic>Holoenzymes - chemistry</topic><topic>Mediator Complex</topic><topic>Medicin och hälsovetenskap</topic><topic>MOLECULAR SEQUENCE DATA</topic><topic>MOLECULAR WEIGHT</topic><topic>Nuclear Proteins - analysis</topic><topic>Nuclear Proteins - genetics</topic><topic>PESO MOLECULAR</topic><topic>POIDS MOLECULAIRE</topic><topic>Precipitin Tests</topic><topic>Protein Binding</topic><topic>PROTEINAS AGLUTINANTES</topic><topic>PROTEINE DE LIAISON</topic><topic>PURIFICACION</topic><topic>PURIFICATION</topic><topic>RNA Polymerase II - chemistry</topic><topic>SACCHAROMYCES CEREVISIAE</topic><topic>Saccharomyces cerevisiae - enzymology</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae Proteins</topic><topic>Sequence Analysis</topic><topic>Sequence Homology, Amino Acid</topic><topic>Transcription Factors</topic><topic>TRANSFERASAS</topic><topic>TRANSFERASE</topic><topic>TRANSFERASES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Gustafsson, Claes M.</creatorcontrib><creatorcontrib>Myers, Lawrence C.</creatorcontrib><creatorcontrib>Beve, Jenny</creatorcontrib><creatorcontrib>Spåhr, Henrik</creatorcontrib><creatorcontrib>Lui, Mary</creatorcontrib><creatorcontrib>Erdjument-Bromage, Hediye</creatorcontrib><creatorcontrib>Tempst, Paul</creatorcontrib><creatorcontrib>Kornberg, Roger D.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SwePub Articles full text</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Gustafsson, Claes M.</au><au>Myers, Lawrence C.</au><au>Beve, Jenny</au><au>Spåhr, Henrik</au><au>Lui, Mary</au><au>Erdjument-Bromage, Hediye</au><au>Tempst, Paul</au><au>Kornberg, Roger D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of New Mediator Subunits in the RNA Polymerase II Holoenzyme from Saccharomyces cerevisiae</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1998-11-20</date><risdate>1998</risdate><volume>273</volume><issue>47</issue><spage>30851</spage><epage>30854</epage><pages>30851-30854</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Mediator was isolated from yeast on the basis of its requirement for transcriptional activation in a fully defined system. 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| identifier | ISSN: 0021-9258 |
| ispartof | The Journal of biological chemistry, 1998-11, Vol.273 (47), p.30851-30854 |
| issn | 0021-9258 1083-351X |
| language | eng |
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| source | MEDLINE; SWEPUB Freely available online; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence AMINO ACID SEQUENCES BINDING PROTEINS CHEMICAL COMPOSITION COMPOSICION QUIMICA COMPOSITION CHIMIQUE Fungal Proteins - analysis Fungal Proteins - genetics Holoenzymes - chemistry Mediator Complex Medicin och hälsovetenskap MOLECULAR SEQUENCE DATA MOLECULAR WEIGHT Nuclear Proteins - analysis Nuclear Proteins - genetics PESO MOLECULAR POIDS MOLECULAIRE Precipitin Tests Protein Binding PROTEINAS AGLUTINANTES PROTEINE DE LIAISON PURIFICACION PURIFICATION RNA Polymerase II - chemistry SACCHAROMYCES CEREVISIAE Saccharomyces cerevisiae - enzymology Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins Sequence Analysis Sequence Homology, Amino Acid Transcription Factors TRANSFERASAS TRANSFERASE TRANSFERASES |
| title | Identification of New Mediator Subunits in the RNA Polymerase II Holoenzyme from Saccharomyces cerevisiae |
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