Solid phase ELISA for determination of the virus dose dependant sensitivity of human cytomegalovirus to antiviral drugs in vitro
The main problems in determining the true in vivo susceptibility of human cytomegalovirus (CMV) to antiviral compounds are the influence of the size of the viral inoculum, the variation in the replication capacity of different CMV strains and the subjective evaluation of the inhibition of viral grow...
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| Veröffentlicht in: | Antiviral research 1998-12, Vol.40 (1), p.105-112 |
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| container_title | Antiviral research |
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| creator | Ljungdahl Ståhle, Ewa Schloss, Lottie Sundqvist, Vivi-Anne Brytting, Maria Hökeberg, Ingegerd Cox, Susan Wahren, Britta Linde, Annika |
| description | The main problems in determining the true in vivo susceptibility of human cytomegalovirus (CMV) to antiviral compounds are the influence of the size of the viral inoculum, the variation in the replication capacity of different CMV strains and the subjective evaluation of the inhibition of viral growth in the plaque assay. In this study, a specific assay was developed which reproducibly determines the sensitivity of primary isolates of CMV. The assay includes simultaneous virus titration and determination of the antiviral sensitivity. When individual virus doses were evaluated, the IC
50 was generally dependent on the virus dose, except for resistant isolates, where the IC
50 did not change irrespective of the dose of virus. The novel method of IC
50 calculation takes into account all values derived from the linear part of the inhibition curve. This may better reflect the in vivo conditions, where the antiviral drug encounters different amounts of virus in different organs. Two human fibroblast-derived cell lines showed similar results. |
| doi_str_mv | 10.1016/S0166-3542(98)00054-0 |
| format | Article |
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50 was generally dependent on the virus dose, except for resistant isolates, where the IC
50 did not change irrespective of the dose of virus. The novel method of IC
50 calculation takes into account all values derived from the linear part of the inhibition curve. This may better reflect the in vivo conditions, where the antiviral drug encounters different amounts of virus in different organs. Two human fibroblast-derived cell lines showed similar results.</description><identifier>ISSN: 0166-3542</identifier><identifier>EISSN: 1872-9096</identifier><identifier>DOI: 10.1016/S0166-3542(98)00054-0</identifier><identifier>CODEN: ARSRDR</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Antiviral drugs ; Antiviral sensitivity assay ; Biological and medical sciences ; Cytomegalovirus ; ELISA ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Human cytomegalovirus ; Medical sciences ; Microbiology ; Pharmacology. Drug treatments ; Techniques used in virology ; Virology</subject><ispartof>Antiviral research, 1998-12, Vol.40 (1), p.105-112</ispartof><rights>1998 Elsevier Science B.V.</rights><rights>1999 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-92fbcac99511ac957f9f21072009deecc474e950f717ae13abb932790c89ada13</citedby><cites>FETCH-LOGICAL-c405t-92fbcac99511ac957f9f21072009deecc474e950f717ae13abb932790c89ada13</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0166354298000540$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3536,27903,27904,65309</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=1661632$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttp://kipublications.ki.se/Default.aspx?queryparsed=id:1957843$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Ljungdahl Ståhle, Ewa</creatorcontrib><creatorcontrib>Schloss, Lottie</creatorcontrib><creatorcontrib>Sundqvist, Vivi-Anne</creatorcontrib><creatorcontrib>Brytting, Maria</creatorcontrib><creatorcontrib>Hökeberg, Ingegerd</creatorcontrib><creatorcontrib>Cox, Susan</creatorcontrib><creatorcontrib>Wahren, Britta</creatorcontrib><creatorcontrib>Linde, Annika</creatorcontrib><title>Solid phase ELISA for determination of the virus dose dependant sensitivity of human cytomegalovirus to antiviral drugs in vitro</title><title>Antiviral research</title><description>The main problems in determining the true in vivo susceptibility of human cytomegalovirus (CMV) to antiviral compounds are the influence of the size of the viral inoculum, the variation in the replication capacity of different CMV strains and the subjective evaluation of the inhibition of viral growth in the plaque assay. In this study, a specific assay was developed which reproducibly determines the sensitivity of primary isolates of CMV. The assay includes simultaneous virus titration and determination of the antiviral sensitivity. When individual virus doses were evaluated, the IC
50 was generally dependent on the virus dose, except for resistant isolates, where the IC
50 did not change irrespective of the dose of virus. The novel method of IC
50 calculation takes into account all values derived from the linear part of the inhibition curve. This may better reflect the in vivo conditions, where the antiviral drug encounters different amounts of virus in different organs. Two human fibroblast-derived cell lines showed similar results.</description><subject>Analysis</subject><subject>Antiviral drugs</subject><subject>Antiviral sensitivity assay</subject><subject>Biological and medical sciences</subject><subject>Cytomegalovirus</subject><subject>ELISA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Human cytomegalovirus</subject><subject>Medical sciences</subject><subject>Microbiology</subject><subject>Pharmacology. 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Psychology</topic><topic>General pharmacology</topic><topic>Human cytomegalovirus</topic><topic>Medical sciences</topic><topic>Microbiology</topic><topic>Pharmacology. 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In this study, a specific assay was developed which reproducibly determines the sensitivity of primary isolates of CMV. The assay includes simultaneous virus titration and determination of the antiviral sensitivity. When individual virus doses were evaluated, the IC
50 was generally dependent on the virus dose, except for resistant isolates, where the IC
50 did not change irrespective of the dose of virus. The novel method of IC
50 calculation takes into account all values derived from the linear part of the inhibition curve. This may better reflect the in vivo conditions, where the antiviral drug encounters different amounts of virus in different organs. Two human fibroblast-derived cell lines showed similar results.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><doi>10.1016/S0166-3542(98)00054-0</doi><tpages>8</tpages></addata></record> |
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| ispartof | Antiviral research, 1998-12, Vol.40 (1), p.105-112 |
| issn | 0166-3542 1872-9096 |
| language | eng |
| recordid | cdi_swepub_primary_oai_swepub_ki_se_432774 |
| source | Elsevier ScienceDirect Journals |
| subjects | Analysis Antiviral drugs Antiviral sensitivity assay Biological and medical sciences Cytomegalovirus ELISA Fundamental and applied biological sciences. Psychology General pharmacology Human cytomegalovirus Medical sciences Microbiology Pharmacology. Drug treatments Techniques used in virology Virology |
| title | Solid phase ELISA for determination of the virus dose dependant sensitivity of human cytomegalovirus to antiviral drugs in vitro |
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