Enhancing saccharification of cassava stems by starch hydrolysis prior to pretreatment

•Cassava stems are a glucan-rich feedstock with a high starch share (up to 42%).•Starch hydrolysis prior to pretreatment improves saccharification of cassava stems.•The hydrolysis of starch minimizes sugar degradation in acid pretreatment.•The starch hydrolysis leads to a two-fold increase of the ov...

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Veröffentlicht in:Industrial crops and products 2017-03, Vol.97, p.21-31
Hauptverfasser: Martín, Carlos, Wei, Maogui, Xiong, Shaojun, Jönsson, Leif J.
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Wei, Maogui
Xiong, Shaojun
Jönsson, Leif J.
description •Cassava stems are a glucan-rich feedstock with a high starch share (up to 42%).•Starch hydrolysis prior to pretreatment improves saccharification of cassava stems.•The hydrolysis of starch minimizes sugar degradation in acid pretreatment.•The starch hydrolysis leads to a two-fold increase of the overall hydrolyzed glucan.•Pretreating cassava stems with [Emim]OAc is more effective than with sulfuric acid. Chemical characterization of cassava stems from different origin revealed that glucans accounted for 54–63% of the dry weight, whereas 35–67% of these glucans consisted of starch. The cassava stems were subjected to a saccharification study including starch hydrolysis, pretreatment with either sulfuric acid or 1-ethyl-3-methylimidazolium acetate ([Emim]OAc), and enzymatic hydrolysis of cellulose. Starch hydrolysis prior to pretreatment decreased sugar degradation, improved enzymatic convertibility of cellulose, and increased overall glucan conversion. Glucan recovery after pretreatment of starch-free cassava stems (SFCS) was around 85%, but below 52% when the stems were pretreated under the same conditions without preparatory starch hydrolysis. The total amount of hydrolyzed glucan after cellulose hydrolysis was two-fold higher for pretreated SFCS than for directly pretreated stems. Pretreatment with [Emim]OAc resulted in 20% higher glucan conversion than pretreatment with acid. Pyrolysis-GC/MS, X-ray diffraction, CP/MAS 13C NMR and FTIR analyses revealed major differences between H2SO4- and [Emim]OAc-pretreated material.
doi_str_mv 10.1016/j.indcrop.2016.11.067
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subjects Cassava stems
Cellulose hydrolysis
Dilute-acid pretreatment
Ethanol
Forest Science
Ionic liquid
Skogsvetenskap
title Enhancing saccharification of cassava stems by starch hydrolysis prior to pretreatment
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