RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design
[Display omitted] ▶ RNAi is particularly successful in the family Saturniidae. ▶ RNAi is particularly successful in genes involved in immunity. ▶ Gene expression in epidermal tissues seems to be most difficult to silence. ▶ Gene silencing by feeding dsRNA requires high concentrations for success. Ge...
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| Veröffentlicht in: | Journal of insect physiology 2011, Vol.57 (2), p.231-245 |
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| creator | Terenius, Olle Papanicolaou, Alexie Garbutt, Jennie S. Eleftherianos, Ioannis Huvenne, Hanneke Kanginakudru, Sriramana Albrechtsen, Merete An, Chunju Aymeric, Jean-Luc Barthel, Andrea Bebas, Piotr Bitra, Kavita Bravo, Alejandra Chevalier, François Collinge, Derek P. Crava, Cristina M. de Maagd, Ruud A. Duvic, Bernard Erlandson, Martin Faye, Ingrid Felföldi, Gabriella Fujiwara, Haruhiko Futahashi, Ryo Gandhe, Archana S. Gatehouse, Heather S. Gatehouse, Laurence N. Giebultowicz, Jadwiga M. Gómez, Isabel Grimmelikhuijzen, Cornelis J.P. Groot, Astrid T. Hauser, Frank Heckel, David G. Hegedus, Dwayne D. Hrycaj, Steven Huang, Lihua Hull, J. Joe Iatrou, Kostas Iga, Masatoshi Kanost, Michael R. Kotwica, Joanna Li, Changyou Li, Jianghong Liu, Jisheng Lundmark, Magnus Matsumoto, Shogo Meyering-Vos, Martina Millichap, Peter J. Monteiro, Antónia Mrinal, Nirotpal Niimi, Teruyuki Nowara, Daniela Ohnishi, Atsushi Oostra, Vicencio Ozaki, Katsuhisa Papakonstantinou, Maria Popadic, Aleksandar Rajam, Manchikatla V. Saenko, Suzanne Simpson, Robert M. Soberón, Mario Strand, Michael R. Tomita, Shuichiro Toprak, Umut Wang, Ping Wee, Choon Wei Whyard, Steven Zhang, Wenqing Nagaraju, Javaregowda ffrench-Constant, Richard H. Herrero, Salvador Gordon, Karl Swevers, Luc Smagghe, Guy |
| description | [Display omitted]
▶ RNAi is particularly successful in the family Saturniidae. ▶ RNAi is particularly successful in genes involved in immunity. ▶ Gene expression in epidermal tissues seems to be most difficult to silence. ▶ Gene silencing by feeding dsRNA requires high concentrations for success.
Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi experiments in Lepidoptera are discussed. The review also points to a need to further investigate the mechanism of RNAi in lepidopteran insects and its possible connection to the innate immune response. Our general understanding of RNAi in Lepidoptera will be further aided in the future as our public database at
http://insectacentral.org/RNAi will continue to gather information on RNAi experiments. |
| doi_str_mv | 10.1016/j.jinsphys.2010.11.006 |
| format | Article |
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▶ RNAi is particularly successful in the family Saturniidae. ▶ RNAi is particularly successful in genes involved in immunity. ▶ Gene expression in epidermal tissues seems to be most difficult to silence. ▶ Gene silencing by feeding dsRNA requires high concentrations for success.
Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi experiments in Lepidoptera are discussed. The review also points to a need to further investigate the mechanism of RNAi in lepidopteran insects and its possible connection to the innate immune response. Our general understanding of RNAi in Lepidoptera will be further aided in the future as our public database at
http://insectacentral.org/RNAi will continue to gather information on RNAi experiments.</description><identifier>ISSN: 0022-1910</identifier><identifier>ISSN: 1879-1611</identifier><identifier>EISSN: 1879-1611</identifier><identifier>DOI: 10.1016/j.jinsphys.2010.11.006</identifier><identifier>PMID: 21078327</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Biochemistry and Molecular Biology ; Biokemi och molekylärbiologi ; Databases, Genetic ; Delivery methods ; dsRNA properties ; Ecology ; Ekologi ; Epidermis - growth & development ; Gene Expression Regulation ; Gene function ; Gene Silencing ; Genetics ; Genetik ; Immunity, Innate ; Insect Proteins - drug effects ; Insect Proteins - genetics ; Insect Proteins - immunology ; Lepidoptera ; Lepidoptera - drug effects ; Lepidoptera - genetics ; Lepidoptera - growth & development ; Lepidoptera - immunology ; Life Sciences ; Research Design ; RNA Interference ; RNA, Double-Stranded - drug effects ; Saturniidae ; Tissue uptake ; Zoologi ; Zoology</subject><ispartof>Journal of insect physiology, 2011, Vol.57 (2), p.231-245</ispartof><rights>2010 Elsevier Ltd</rights><rights>Copyright © 2010 Elsevier Ltd. All rights reserved.</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c698t-87f56cffb7e1a8df0732e6d021a88a1b983e8fd4c02766ce6422002157cbd7903</citedby><cites>FETCH-LOGICAL-c698t-87f56cffb7e1a8df0732e6d021a88a1b983e8fd4c02766ce6422002157cbd7903</cites><orcidid>0000-0002-7618-7719 ; 0000-0001-7991-3045 ; 0000-0001-5690-2108 ; 0000-0002-1273-1906 ; 0000-0001-5340-6774</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0022191010003057$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>230,314,776,780,881,3537,4010,27900,27901,27902,65306</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21078327$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.inrae.fr/hal-02652706$$DView record in HAL$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:su:diva-52967$$DView record from Swedish Publication Index$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-499445$$DView record from Swedish Publication Index$$Hfree_for_read</backlink><backlink>$$Uhttps://res.slu.se/id/publ/59494$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Terenius, Olle</creatorcontrib><creatorcontrib>Papanicolaou, Alexie</creatorcontrib><creatorcontrib>Garbutt, Jennie S.</creatorcontrib><creatorcontrib>Eleftherianos, Ioannis</creatorcontrib><creatorcontrib>Huvenne, Hanneke</creatorcontrib><creatorcontrib>Kanginakudru, Sriramana</creatorcontrib><creatorcontrib>Albrechtsen, Merete</creatorcontrib><creatorcontrib>An, Chunju</creatorcontrib><creatorcontrib>Aymeric, Jean-Luc</creatorcontrib><creatorcontrib>Barthel, Andrea</creatorcontrib><creatorcontrib>Bebas, Piotr</creatorcontrib><creatorcontrib>Bitra, Kavita</creatorcontrib><creatorcontrib>Bravo, Alejandra</creatorcontrib><creatorcontrib>Chevalier, François</creatorcontrib><creatorcontrib>Collinge, Derek P.</creatorcontrib><creatorcontrib>Crava, Cristina M.</creatorcontrib><creatorcontrib>de Maagd, Ruud A.</creatorcontrib><creatorcontrib>Duvic, Bernard</creatorcontrib><creatorcontrib>Erlandson, Martin</creatorcontrib><creatorcontrib>Faye, Ingrid</creatorcontrib><creatorcontrib>Felföldi, Gabriella</creatorcontrib><creatorcontrib>Fujiwara, Haruhiko</creatorcontrib><creatorcontrib>Futahashi, Ryo</creatorcontrib><creatorcontrib>Gandhe, Archana S.</creatorcontrib><creatorcontrib>Gatehouse, Heather S.</creatorcontrib><creatorcontrib>Gatehouse, Laurence N.</creatorcontrib><creatorcontrib>Giebultowicz, Jadwiga M.</creatorcontrib><creatorcontrib>Gómez, Isabel</creatorcontrib><creatorcontrib>Grimmelikhuijzen, Cornelis J.P.</creatorcontrib><creatorcontrib>Groot, Astrid T.</creatorcontrib><creatorcontrib>Hauser, Frank</creatorcontrib><creatorcontrib>Heckel, David G.</creatorcontrib><creatorcontrib>Hegedus, Dwayne D.</creatorcontrib><creatorcontrib>Hrycaj, Steven</creatorcontrib><creatorcontrib>Huang, Lihua</creatorcontrib><creatorcontrib>Hull, J. Joe</creatorcontrib><creatorcontrib>Iatrou, Kostas</creatorcontrib><creatorcontrib>Iga, Masatoshi</creatorcontrib><creatorcontrib>Kanost, Michael R.</creatorcontrib><creatorcontrib>Kotwica, Joanna</creatorcontrib><creatorcontrib>Li, Changyou</creatorcontrib><creatorcontrib>Li, Jianghong</creatorcontrib><creatorcontrib>Liu, Jisheng</creatorcontrib><creatorcontrib>Lundmark, Magnus</creatorcontrib><creatorcontrib>Matsumoto, Shogo</creatorcontrib><creatorcontrib>Meyering-Vos, Martina</creatorcontrib><creatorcontrib>Millichap, Peter J.</creatorcontrib><creatorcontrib>Monteiro, Antónia</creatorcontrib><creatorcontrib>Mrinal, Nirotpal</creatorcontrib><creatorcontrib>Niimi, Teruyuki</creatorcontrib><creatorcontrib>Nowara, Daniela</creatorcontrib><creatorcontrib>Ohnishi, Atsushi</creatorcontrib><creatorcontrib>Oostra, Vicencio</creatorcontrib><creatorcontrib>Ozaki, Katsuhisa</creatorcontrib><creatorcontrib>Papakonstantinou, Maria</creatorcontrib><creatorcontrib>Popadic, Aleksandar</creatorcontrib><creatorcontrib>Rajam, Manchikatla V.</creatorcontrib><creatorcontrib>Saenko, Suzanne</creatorcontrib><creatorcontrib>Simpson, Robert M.</creatorcontrib><creatorcontrib>Soberón, Mario</creatorcontrib><creatorcontrib>Strand, Michael R.</creatorcontrib><creatorcontrib>Tomita, Shuichiro</creatorcontrib><creatorcontrib>Toprak, Umut</creatorcontrib><creatorcontrib>Wang, Ping</creatorcontrib><creatorcontrib>Wee, Choon Wei</creatorcontrib><creatorcontrib>Whyard, Steven</creatorcontrib><creatorcontrib>Zhang, Wenqing</creatorcontrib><creatorcontrib>Nagaraju, Javaregowda</creatorcontrib><creatorcontrib>ffrench-Constant, Richard H.</creatorcontrib><creatorcontrib>Herrero, Salvador</creatorcontrib><creatorcontrib>Gordon, Karl</creatorcontrib><creatorcontrib>Swevers, Luc</creatorcontrib><creatorcontrib>Smagghe, Guy</creatorcontrib><creatorcontrib>Sveriges lantbruksuniversitet</creatorcontrib><title>RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design</title><title>Journal of insect physiology</title><addtitle>J Insect Physiol</addtitle><description>[Display omitted]
▶ RNAi is particularly successful in the family Saturniidae. ▶ RNAi is particularly successful in genes involved in immunity. ▶ Gene expression in epidermal tissues seems to be most difficult to silence. ▶ Gene silencing by feeding dsRNA requires high concentrations for success.
Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi experiments in Lepidoptera are discussed. The review also points to a need to further investigate the mechanism of RNAi in lepidopteran insects and its possible connection to the innate immune response. Our general understanding of RNAi in Lepidoptera will be further aided in the future as our public database at
http://insectacentral.org/RNAi will continue to gather information on RNAi experiments.</description><subject>Animals</subject><subject>Biochemistry and Molecular Biology</subject><subject>Biokemi och molekylärbiologi</subject><subject>Databases, Genetic</subject><subject>Delivery methods</subject><subject>dsRNA properties</subject><subject>Ecology</subject><subject>Ekologi</subject><subject>Epidermis - growth & development</subject><subject>Gene Expression Regulation</subject><subject>Gene function</subject><subject>Gene Silencing</subject><subject>Genetics</subject><subject>Genetik</subject><subject>Immunity, Innate</subject><subject>Insect Proteins - drug effects</subject><subject>Insect Proteins - genetics</subject><subject>Insect Proteins - immunology</subject><subject>Lepidoptera</subject><subject>Lepidoptera - drug effects</subject><subject>Lepidoptera - genetics</subject><subject>Lepidoptera - growth & development</subject><subject>Lepidoptera - immunology</subject><subject>Life Sciences</subject><subject>Research Design</subject><subject>RNA Interference</subject><subject>RNA, Double-Stranded - drug effects</subject><subject>Saturniidae</subject><subject>Tissue uptake</subject><subject>Zoologi</subject><subject>Zoology</subject><issn>0022-1910</issn><issn>1879-1611</issn><issn>1879-1611</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkstu1DAUhiMEokPhFUp2CEEG20l8YcWoUIo0AgkoW8txjqceZeJgx1Mq8fA4TVvBAs3q6Pz-zsX2n2UnGC0xwvTNdrm1fRgur8OSoEnES4Tog2yBORMFphg_zBYIEVJggdFR9iSELUKoprx-nB0RjBgvCVtkv79-XuW2H8Eb8NBrSEm-hsG2bkiiepuv-tztwe8tXOXO5CFqDSGY2OWqb_PY_yWEMbYWws2B3Q2d1Wq0rg-5cT6HXwN4u4N-VF3eQrCb_mn2yKguwLPbeJxdnH34fnperL98_HS6WheaCj4WnJmaamMaBljx1iBWEqAtIinjCjeCl8BNW2lEGKUaaEVIujqumW5aJlB5nBVz33AFQ2zkkPZQ_lo6ZWXoYqP8FGQAWYtKVIl__V_-vf2xks5vZIyyEqKq6oS_OoyHKGsiKEv0y5m-VN0_6PlqLScNEVoThugeJ_bFzA7e_YwQRrmzQUPXqR5cDJJTVDLGaXWYrMoKE0qm-XQmtXcheDD3S2AkJ2_Jrbzzlpy8JTGWyVup8OR2RGx20N6X3ZkpAc9nwCgn1cbbIC--pQ4lwqLiGJFEvJsJSL-dHJXeXdvJda31oEfZOntoiz9YTu8Q</recordid><startdate>2011</startdate><enddate>2011</enddate><creator>Terenius, Olle</creator><creator>Papanicolaou, Alexie</creator><creator>Garbutt, Jennie S.</creator><creator>Eleftherianos, Ioannis</creator><creator>Huvenne, Hanneke</creator><creator>Kanginakudru, Sriramana</creator><creator>Albrechtsen, Merete</creator><creator>An, Chunju</creator><creator>Aymeric, Jean-Luc</creator><creator>Barthel, Andrea</creator><creator>Bebas, Piotr</creator><creator>Bitra, Kavita</creator><creator>Bravo, Alejandra</creator><creator>Chevalier, François</creator><creator>Collinge, Derek P.</creator><creator>Crava, Cristina M.</creator><creator>de Maagd, Ruud A.</creator><creator>Duvic, Bernard</creator><creator>Erlandson, Martin</creator><creator>Faye, Ingrid</creator><creator>Felföldi, Gabriella</creator><creator>Fujiwara, Haruhiko</creator><creator>Futahashi, Ryo</creator><creator>Gandhe, Archana S.</creator><creator>Gatehouse, Heather S.</creator><creator>Gatehouse, Laurence N.</creator><creator>Giebultowicz, Jadwiga M.</creator><creator>Gómez, Isabel</creator><creator>Grimmelikhuijzen, Cornelis J.P.</creator><creator>Groot, Astrid T.</creator><creator>Hauser, Frank</creator><creator>Heckel, David G.</creator><creator>Hegedus, Dwayne D.</creator><creator>Hrycaj, Steven</creator><creator>Huang, Lihua</creator><creator>Hull, J. 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Joe ; Iatrou, Kostas ; Iga, Masatoshi ; Kanost, Michael R. ; Kotwica, Joanna ; Li, Changyou ; Li, Jianghong ; Liu, Jisheng ; Lundmark, Magnus ; Matsumoto, Shogo ; Meyering-Vos, Martina ; Millichap, Peter J. ; Monteiro, Antónia ; Mrinal, Nirotpal ; Niimi, Teruyuki ; Nowara, Daniela ; Ohnishi, Atsushi ; Oostra, Vicencio ; Ozaki, Katsuhisa ; Papakonstantinou, Maria ; Popadic, Aleksandar ; Rajam, Manchikatla V. ; Saenko, Suzanne ; Simpson, Robert M. ; Soberón, Mario ; Strand, Michael R. ; Tomita, Shuichiro ; Toprak, Umut ; Wang, Ping ; Wee, Choon Wei ; Whyard, Steven ; Zhang, Wenqing ; Nagaraju, Javaregowda ; ffrench-Constant, Richard H. ; Herrero, Salvador ; Gordon, Karl ; Swevers, Luc ; Smagghe, Guy</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c698t-87f56cffb7e1a8df0732e6d021a88a1b983e8fd4c02766ce6422002157cbd7903</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Biochemistry and Molecular Biology</topic><topic>Biokemi och molekylärbiologi</topic><topic>Databases, Genetic</topic><topic>Delivery methods</topic><topic>dsRNA properties</topic><topic>Ecology</topic><topic>Ekologi</topic><topic>Epidermis - growth & development</topic><topic>Gene Expression Regulation</topic><topic>Gene function</topic><topic>Gene Silencing</topic><topic>Genetics</topic><topic>Genetik</topic><topic>Immunity, Innate</topic><topic>Insect Proteins - drug effects</topic><topic>Insect Proteins - genetics</topic><topic>Insect Proteins - immunology</topic><topic>Lepidoptera</topic><topic>Lepidoptera - drug effects</topic><topic>Lepidoptera - genetics</topic><topic>Lepidoptera - growth & development</topic><topic>Lepidoptera - immunology</topic><topic>Life Sciences</topic><topic>Research Design</topic><topic>RNA Interference</topic><topic>RNA, Double-Stranded - drug effects</topic><topic>Saturniidae</topic><topic>Tissue uptake</topic><topic>Zoologi</topic><topic>Zoology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Terenius, Olle</creatorcontrib><creatorcontrib>Papanicolaou, Alexie</creatorcontrib><creatorcontrib>Garbutt, Jennie S.</creatorcontrib><creatorcontrib>Eleftherianos, Ioannis</creatorcontrib><creatorcontrib>Huvenne, Hanneke</creatorcontrib><creatorcontrib>Kanginakudru, Sriramana</creatorcontrib><creatorcontrib>Albrechtsen, Merete</creatorcontrib><creatorcontrib>An, Chunju</creatorcontrib><creatorcontrib>Aymeric, Jean-Luc</creatorcontrib><creatorcontrib>Barthel, Andrea</creatorcontrib><creatorcontrib>Bebas, Piotr</creatorcontrib><creatorcontrib>Bitra, Kavita</creatorcontrib><creatorcontrib>Bravo, Alejandra</creatorcontrib><creatorcontrib>Chevalier, François</creatorcontrib><creatorcontrib>Collinge, Derek P.</creatorcontrib><creatorcontrib>Crava, Cristina M.</creatorcontrib><creatorcontrib>de Maagd, Ruud A.</creatorcontrib><creatorcontrib>Duvic, Bernard</creatorcontrib><creatorcontrib>Erlandson, Martin</creatorcontrib><creatorcontrib>Faye, Ingrid</creatorcontrib><creatorcontrib>Felföldi, Gabriella</creatorcontrib><creatorcontrib>Fujiwara, Haruhiko</creatorcontrib><creatorcontrib>Futahashi, Ryo</creatorcontrib><creatorcontrib>Gandhe, Archana S.</creatorcontrib><creatorcontrib>Gatehouse, Heather S.</creatorcontrib><creatorcontrib>Gatehouse, Laurence N.</creatorcontrib><creatorcontrib>Giebultowicz, Jadwiga M.</creatorcontrib><creatorcontrib>Gómez, Isabel</creatorcontrib><creatorcontrib>Grimmelikhuijzen, Cornelis J.P.</creatorcontrib><creatorcontrib>Groot, Astrid T.</creatorcontrib><creatorcontrib>Hauser, Frank</creatorcontrib><creatorcontrib>Heckel, David G.</creatorcontrib><creatorcontrib>Hegedus, Dwayne D.</creatorcontrib><creatorcontrib>Hrycaj, Steven</creatorcontrib><creatorcontrib>Huang, Lihua</creatorcontrib><creatorcontrib>Hull, J. Joe</creatorcontrib><creatorcontrib>Iatrou, Kostas</creatorcontrib><creatorcontrib>Iga, Masatoshi</creatorcontrib><creatorcontrib>Kanost, Michael R.</creatorcontrib><creatorcontrib>Kotwica, Joanna</creatorcontrib><creatorcontrib>Li, Changyou</creatorcontrib><creatorcontrib>Li, Jianghong</creatorcontrib><creatorcontrib>Liu, Jisheng</creatorcontrib><creatorcontrib>Lundmark, Magnus</creatorcontrib><creatorcontrib>Matsumoto, Shogo</creatorcontrib><creatorcontrib>Meyering-Vos, Martina</creatorcontrib><creatorcontrib>Millichap, Peter J.</creatorcontrib><creatorcontrib>Monteiro, Antónia</creatorcontrib><creatorcontrib>Mrinal, Nirotpal</creatorcontrib><creatorcontrib>Niimi, Teruyuki</creatorcontrib><creatorcontrib>Nowara, Daniela</creatorcontrib><creatorcontrib>Ohnishi, Atsushi</creatorcontrib><creatorcontrib>Oostra, Vicencio</creatorcontrib><creatorcontrib>Ozaki, Katsuhisa</creatorcontrib><creatorcontrib>Papakonstantinou, Maria</creatorcontrib><creatorcontrib>Popadic, Aleksandar</creatorcontrib><creatorcontrib>Rajam, Manchikatla V.</creatorcontrib><creatorcontrib>Saenko, Suzanne</creatorcontrib><creatorcontrib>Simpson, Robert M.</creatorcontrib><creatorcontrib>Soberón, Mario</creatorcontrib><creatorcontrib>Strand, Michael R.</creatorcontrib><creatorcontrib>Tomita, Shuichiro</creatorcontrib><creatorcontrib>Toprak, Umut</creatorcontrib><creatorcontrib>Wang, Ping</creatorcontrib><creatorcontrib>Wee, Choon Wei</creatorcontrib><creatorcontrib>Whyard, Steven</creatorcontrib><creatorcontrib>Zhang, Wenqing</creatorcontrib><creatorcontrib>Nagaraju, Javaregowda</creatorcontrib><creatorcontrib>ffrench-Constant, Richard H.</creatorcontrib><creatorcontrib>Herrero, Salvador</creatorcontrib><creatorcontrib>Gordon, Karl</creatorcontrib><creatorcontrib>Swevers, Luc</creatorcontrib><creatorcontrib>Smagghe, Guy</creatorcontrib><creatorcontrib>Sveriges lantbruksuniversitet</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Nucleic Acids Abstracts</collection><collection>Hyper Article en Ligne (HAL)</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Stockholms universitet</collection><collection>SWEPUB Uppsala universitet</collection><jtitle>Journal of insect physiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Terenius, Olle</au><au>Papanicolaou, Alexie</au><au>Garbutt, Jennie S.</au><au>Eleftherianos, Ioannis</au><au>Huvenne, Hanneke</au><au>Kanginakudru, Sriramana</au><au>Albrechtsen, Merete</au><au>An, Chunju</au><au>Aymeric, Jean-Luc</au><au>Barthel, Andrea</au><au>Bebas, Piotr</au><au>Bitra, Kavita</au><au>Bravo, Alejandra</au><au>Chevalier, François</au><au>Collinge, Derek P.</au><au>Crava, Cristina M.</au><au>de Maagd, Ruud A.</au><au>Duvic, Bernard</au><au>Erlandson, Martin</au><au>Faye, Ingrid</au><au>Felföldi, Gabriella</au><au>Fujiwara, Haruhiko</au><au>Futahashi, Ryo</au><au>Gandhe, Archana S.</au><au>Gatehouse, Heather S.</au><au>Gatehouse, Laurence N.</au><au>Giebultowicz, Jadwiga M.</au><au>Gómez, Isabel</au><au>Grimmelikhuijzen, Cornelis J.P.</au><au>Groot, Astrid T.</au><au>Hauser, Frank</au><au>Heckel, David G.</au><au>Hegedus, Dwayne D.</au><au>Hrycaj, Steven</au><au>Huang, Lihua</au><au>Hull, J. Joe</au><au>Iatrou, Kostas</au><au>Iga, Masatoshi</au><au>Kanost, Michael R.</au><au>Kotwica, Joanna</au><au>Li, Changyou</au><au>Li, Jianghong</au><au>Liu, Jisheng</au><au>Lundmark, Magnus</au><au>Matsumoto, Shogo</au><au>Meyering-Vos, Martina</au><au>Millichap, Peter J.</au><au>Monteiro, Antónia</au><au>Mrinal, Nirotpal</au><au>Niimi, Teruyuki</au><au>Nowara, Daniela</au><au>Ohnishi, Atsushi</au><au>Oostra, Vicencio</au><au>Ozaki, Katsuhisa</au><au>Papakonstantinou, Maria</au><au>Popadic, Aleksandar</au><au>Rajam, Manchikatla V.</au><au>Saenko, Suzanne</au><au>Simpson, Robert M.</au><au>Soberón, Mario</au><au>Strand, Michael R.</au><au>Tomita, Shuichiro</au><au>Toprak, Umut</au><au>Wang, Ping</au><au>Wee, Choon Wei</au><au>Whyard, Steven</au><au>Zhang, Wenqing</au><au>Nagaraju, Javaregowda</au><au>ffrench-Constant, Richard H.</au><au>Herrero, Salvador</au><au>Gordon, Karl</au><au>Swevers, Luc</au><au>Smagghe, Guy</au><aucorp>Sveriges lantbruksuniversitet</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design</atitle><jtitle>Journal of insect physiology</jtitle><addtitle>J Insect Physiol</addtitle><date>2011</date><risdate>2011</risdate><volume>57</volume><issue>2</issue><spage>231</spage><epage>245</epage><pages>231-245</pages><issn>0022-1910</issn><issn>1879-1611</issn><eissn>1879-1611</eissn><abstract>[Display omitted]
▶ RNAi is particularly successful in the family Saturniidae. ▶ RNAi is particularly successful in genes involved in immunity. ▶ Gene expression in epidermal tissues seems to be most difficult to silence. ▶ Gene silencing by feeding dsRNA requires high concentrations for success.
Gene silencing through RNA interference (RNAi) has revolutionized the study of gene function, particularly in non-model insects. However, in Lepidoptera (moths and butterflies) RNAi has many times proven to be difficult to achieve. Most of the negative results have been anecdotal and the positive experiments have not been collected in such a way that they are possible to analyze. In this review, we have collected detailed data from more than 150 experiments including all to date published and many unpublished experiments. Despite a large variation in the data, trends that are found are that RNAi is particularly successful in the family Saturniidae and in genes involved in immunity. On the contrary, gene expression in epidermal tissues seems to be most difficult to silence. In addition, gene silencing by feeding dsRNA requires high concentrations for success. Possible causes for the variability of success in RNAi experiments in Lepidoptera are discussed. The review also points to a need to further investigate the mechanism of RNAi in lepidopteran insects and its possible connection to the innate immune response. Our general understanding of RNAi in Lepidoptera will be further aided in the future as our public database at
http://insectacentral.org/RNAi will continue to gather information on RNAi experiments.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>21078327</pmid><doi>10.1016/j.jinsphys.2010.11.006</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-7618-7719</orcidid><orcidid>https://orcid.org/0000-0001-7991-3045</orcidid><orcidid>https://orcid.org/0000-0001-5690-2108</orcidid><orcidid>https://orcid.org/0000-0002-1273-1906</orcidid><orcidid>https://orcid.org/0000-0001-5340-6774</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-1910 |
| ispartof | Journal of insect physiology, 2011, Vol.57 (2), p.231-245 |
| issn | 0022-1910 1879-1611 1879-1611 |
| language | eng |
| recordid | cdi_swepub_primary_oai_slubar_slu_se_59494 |
| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Animals Biochemistry and Molecular Biology Biokemi och molekylärbiologi Databases, Genetic Delivery methods dsRNA properties Ecology Ekologi Epidermis - growth & development Gene Expression Regulation Gene function Gene Silencing Genetics Genetik Immunity, Innate Insect Proteins - drug effects Insect Proteins - genetics Insect Proteins - immunology Lepidoptera Lepidoptera - drug effects Lepidoptera - genetics Lepidoptera - growth & development Lepidoptera - immunology Life Sciences Research Design RNA Interference RNA, Double-Stranded - drug effects Saturniidae Tissue uptake Zoologi Zoology |
| title | RNA interference in Lepidoptera: An overview of successful and unsuccessful studies and implications for experimental design |
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