Segmented filamentous bacteria in human ileostomy samples after high-fiber intake
Abstract Segmented filamentous bacteria (SFB) are inhabitants of the small intestine of various animals, where they can be detected microscopically due to their specific morphology and intimate association with the intestinal epithelium. SFB colonize the distal part of the small intestine in a host-...
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| Veröffentlicht in: | FEMS microbiology letters 2013-05, Vol.342 (1), p.24-29 |
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Segmented filamentous bacteria (SFB) are inhabitants of the small intestine of various animals, where they can be detected microscopically due to their specific morphology and intimate association with the intestinal epithelium. SFB colonize the distal part of the small intestine in a host-specific manner and affects important functions of the immune system, such as the induction of secretory IgA production and regulation of T-cell maturation. Considering the influences SFB have on immune functions, they could be regarded as a key species in host–microbial interactions of the gastrointestinal tract. Although these influences might be executed by other microorganisms, a human-adapted variant of SFB is not unlikely. In this study, ileostomy samples from 10 human subjects were screened with PCR, using primers derived from sequences of SFB from rat and mouse. PCR products were obtained from samples taken from one individual at two time points. Sequencing revealed the presence of a 16S rRNA gene with high similarity (98%) to the corresponding genes from SFB of mouse and rat origin, thus indicating the presence of a human variant of SFB. The findings presented in this study will hopefully encourage research to elucidate whether this intriguing organism is a persistent member of the normal human microbiota. |
| doi_str_mv | 10.1111/1574-6968.12103 |
| format | Article |
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Segmented filamentous bacteria (SFB) are inhabitants of the small intestine of various animals, where they can be detected microscopically due to their specific morphology and intimate association with the intestinal epithelium. SFB colonize the distal part of the small intestine in a host-specific manner and affects important functions of the immune system, such as the induction of secretory IgA production and regulation of T-cell maturation. Considering the influences SFB have on immune functions, they could be regarded as a key species in host–microbial interactions of the gastrointestinal tract. Although these influences might be executed by other microorganisms, a human-adapted variant of SFB is not unlikely. In this study, ileostomy samples from 10 human subjects were screened with PCR, using primers derived from sequences of SFB from rat and mouse. PCR products were obtained from samples taken from one individual at two time points. Sequencing revealed the presence of a 16S rRNA gene with high similarity (98%) to the corresponding genes from SFB of mouse and rat origin, thus indicating the presence of a human variant of SFB. The findings presented in this study will hopefully encourage research to elucidate whether this intriguing organism is a persistent member of the normal human microbiota.</description><identifier>ISSN: 0378-1097</identifier><identifier>ISSN: 1574-6968</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1111/1574-6968.12103</identifier><identifier>PMID: 23406300</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Adult ; Animals ; Bacteria ; Bacteria - classification ; Bacteria - genetics ; Bacteria - isolation & purification ; Bacteriology ; Biological and medical sciences ; Cluster Analysis ; Dietary Fiber - administration & dosage ; dietary impact ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; DNA, Ribosomal - chemistry ; DNA, Ribosomal - genetics ; Fundamental and applied biological sciences. Psychology ; Gastrointestinal tract ; Gastrointestinal Tract - microbiology ; host–bacterial interactions ; Humans ; Ileostomy ; Immune system ; intestinal microbiota ; Mice ; Microbiology ; Microorganisms ; Mikrobiologi ; Miscellaneous ; Molecular Sequence Data ; Ostomy ; Phylogeny ; Polymerase Chain Reaction ; Rats ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Small intestine ; symbiosis</subject><ispartof>FEMS microbiology letters, 2013-05, Vol.342 (1), p.24-29</ispartof><rights>Copyright © 2013 Federation of European Microbiological Societies: Published by Blackwell Publishing Ltd 2013</rights><rights>2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved</rights><rights>2014 INIST-CNRS</rights><rights>2013 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved.</rights><rights>Copyright © 2013 Federation of European Microbiological Societies: Published by Blackwell Publishing Ltd</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5483-f723c01ac91c7471ca953de93031471803b0cac6e2ee326b2f709cdcaad34e2a3</citedby><cites>FETCH-LOGICAL-c5483-f723c01ac91c7471ca953de93031471803b0cac6e2ee326b2f709cdcaad34e2a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2F1574-6968.12103$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2F1574-6968.12103$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>230,314,780,784,885,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=27216747$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23406300$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://res.slu.se/id/publ/53148$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Jonsson, Hans</creatorcontrib><creatorcontrib>Sveriges lantbruksuniversitet</creatorcontrib><title>Segmented filamentous bacteria in human ileostomy samples after high-fiber intake</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>Abstract
Segmented filamentous bacteria (SFB) are inhabitants of the small intestine of various animals, where they can be detected microscopically due to their specific morphology and intimate association with the intestinal epithelium. SFB colonize the distal part of the small intestine in a host-specific manner and affects important functions of the immune system, such as the induction of secretory IgA production and regulation of T-cell maturation. Considering the influences SFB have on immune functions, they could be regarded as a key species in host–microbial interactions of the gastrointestinal tract. Although these influences might be executed by other microorganisms, a human-adapted variant of SFB is not unlikely. In this study, ileostomy samples from 10 human subjects were screened with PCR, using primers derived from sequences of SFB from rat and mouse. PCR products were obtained from samples taken from one individual at two time points. Sequencing revealed the presence of a 16S rRNA gene with high similarity (98%) to the corresponding genes from SFB of mouse and rat origin, thus indicating the presence of a human variant of SFB. The findings presented in this study will hopefully encourage research to elucidate whether this intriguing organism is a persistent member of the normal human microbiota.</description><subject>Adult</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Cluster Analysis</subject><subject>Dietary Fiber - administration & dosage</subject><subject>dietary impact</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA, Ribosomal - chemistry</subject><subject>DNA, Ribosomal - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gastrointestinal tract</subject><subject>Gastrointestinal Tract - microbiology</subject><subject>host–bacterial interactions</subject><subject>Humans</subject><subject>Ileostomy</subject><subject>Immune system</subject><subject>intestinal microbiota</subject><subject>Mice</subject><subject>Microbiology</subject><subject>Microorganisms</subject><subject>Mikrobiologi</subject><subject>Miscellaneous</subject><subject>Molecular Sequence Data</subject><subject>Ostomy</subject><subject>Phylogeny</subject><subject>Polymerase Chain Reaction</subject><subject>Rats</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Small intestine</subject><subject>symbiosis</subject><issn>0378-1097</issn><issn>1574-6968</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkd-L1DAQx4Mo3t7ps29SEOEQejdJ2qR9lMNTYUVEfQ7TdHqbs79MWo79703t3ioiaF5mJnzmOzN8GXvG4YLHd8lznaWqVMUFFxzkA7Y5_jxkG5C6SDmU-oSdhnALAJkA9ZidCJmBkgAb9ukz3XTUT1QnjWtxSYc5JBXaibzDxPXJbu6wT1xLQ5iGbp8E7MaWQoJNRJKdu9mljati6voJv9ET9qjBNtDTQzxjX6_ffLl6l24_vn1_9Xqb2jwrZNpoIS1wtCW3OtPcYpnLmkoJkseyAFmBRatIEEmhKtFoKG1tEWuZkUB5xtJVN9zROFdm9K5DvzcDOhPauUK_BBPI5FGxiPz5yo9--D5TmEzngqW2xZ7iyYZLpSNWaPUfqFBacsV5RF_8gd4Os-_j4YbnErgshdCRulwp64cQPDXHbTmYxUiz2GYW28xPI2PH84PuXHVUH_l75yLw8gBgsNg2Hnvrwi9OCx7vWUbnK3cXHdz_a665_rC9X-DV2jfM41-70t-2_QF_TsAH</recordid><startdate>201305</startdate><enddate>201305</enddate><creator>Jonsson, Hans</creator><general>Blackwell Publishing Ltd</general><general>Wiley-Blackwell</general><general>Oxford University Press</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7T7</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>K9.</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>ADTPV</scope><scope>AOWAS</scope></search><sort><creationdate>201305</creationdate><title>Segmented filamentous bacteria in human ileostomy samples after high-fiber intake</title><author>Jonsson, Hans</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5483-f723c01ac91c7471ca953de93031471803b0cac6e2ee326b2f709cdcaad34e2a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adult</topic><topic>Animals</topic><topic>Bacteria</topic><topic>Bacteria - classification</topic><topic>Bacteria - genetics</topic><topic>Bacteria - isolation & purification</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Cluster Analysis</topic><topic>Dietary Fiber - administration & dosage</topic><topic>dietary impact</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA, Ribosomal - chemistry</topic><topic>DNA, Ribosomal - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gastrointestinal tract</topic><topic>Gastrointestinal Tract - microbiology</topic><topic>host–bacterial interactions</topic><topic>Humans</topic><topic>Ileostomy</topic><topic>Immune system</topic><topic>intestinal microbiota</topic><topic>Mice</topic><topic>Microbiology</topic><topic>Microorganisms</topic><topic>Mikrobiologi</topic><topic>Miscellaneous</topic><topic>Molecular Sequence Data</topic><topic>Ostomy</topic><topic>Phylogeny</topic><topic>Polymerase Chain Reaction</topic><topic>Rats</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Small intestine</topic><topic>symbiosis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jonsson, Hans</creatorcontrib><creatorcontrib>Sveriges lantbruksuniversitet</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>SwePub</collection><collection>SwePub Articles</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jonsson, Hans</au><aucorp>Sveriges lantbruksuniversitet</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Segmented filamentous bacteria in human ileostomy samples after high-fiber intake</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2013-05</date><risdate>2013</risdate><volume>342</volume><issue>1</issue><spage>24</spage><epage>29</epage><pages>24-29</pages><issn>0378-1097</issn><issn>1574-6968</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>Abstract
Segmented filamentous bacteria (SFB) are inhabitants of the small intestine of various animals, where they can be detected microscopically due to their specific morphology and intimate association with the intestinal epithelium. SFB colonize the distal part of the small intestine in a host-specific manner and affects important functions of the immune system, such as the induction of secretory IgA production and regulation of T-cell maturation. Considering the influences SFB have on immune functions, they could be regarded as a key species in host–microbial interactions of the gastrointestinal tract. Although these influences might be executed by other microorganisms, a human-adapted variant of SFB is not unlikely. In this study, ileostomy samples from 10 human subjects were screened with PCR, using primers derived from sequences of SFB from rat and mouse. PCR products were obtained from samples taken from one individual at two time points. Sequencing revealed the presence of a 16S rRNA gene with high similarity (98%) to the corresponding genes from SFB of mouse and rat origin, thus indicating the presence of a human variant of SFB. The findings presented in this study will hopefully encourage research to elucidate whether this intriguing organism is a persistent member of the normal human microbiota.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>23406300</pmid><doi>10.1111/1574-6968.12103</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2013-05, Vol.342 (1), p.24-29 |
| issn | 0378-1097 1574-6968 1574-6968 |
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| source | MEDLINE; Access via Wiley Online Library; Oxford University Press Journals All Titles (1996-Current) |
| subjects | Adult Animals Bacteria Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Bacteriology Biological and medical sciences Cluster Analysis Dietary Fiber - administration & dosage dietary impact DNA, Bacterial - chemistry DNA, Bacterial - genetics DNA, Ribosomal - chemistry DNA, Ribosomal - genetics Fundamental and applied biological sciences. Psychology Gastrointestinal tract Gastrointestinal Tract - microbiology host–bacterial interactions Humans Ileostomy Immune system intestinal microbiota Mice Microbiology Microorganisms Mikrobiologi Miscellaneous Molecular Sequence Data Ostomy Phylogeny Polymerase Chain Reaction Rats RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Small intestine symbiosis |
| title | Segmented filamentous bacteria in human ileostomy samples after high-fiber intake |
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