Development of a gas chromatography/mass spectrometry based metabolomics protocol by means of statistical experimental design
Metabolomics is a growing research field where new protocols are rapidly developed and new applications discovered. Common applications include biomarker discovery and elucidation of drug metabolism. However, the development of such protocols rarely includes a systematic optimization followed by val...
Gespeichert in:
| Veröffentlicht in: | Metabolomics 2012-02, Vol.8 (1), p.50-63 |
|---|---|
| Hauptverfasser: | , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 63 |
|---|---|
| container_issue | 1 |
| container_start_page | 50 |
| container_title | Metabolomics |
| container_volume | 8 |
| creator | Danielsson, Anders P. H. Moritz, Thomas Mulder, Hindrik Spégel, Peter |
| description | Metabolomics is a growing research field where new protocols are rapidly developed and new applications discovered. Common applications include biomarker discovery and elucidation of drug metabolism. However, the development of such protocols rarely includes a systematic optimization followed by validation with real samples. Here a GC/MS-based protocol using methoximation followed by silylation with
N
-
tert
-butyldimethylsilyl-
N
-methyltrifluoroacetamide (MTBSTFA) for analysis of blood plasma metabolites is thoroughly developed and optimized from derivatization to detection with statistical design of experiments (DOE). Validation was performed with blood plasma samples and proved the methodology to be efficient, rapid and reliable with a total of 51 analyses performed in 24 h, with linear responses, low detection limits and good precision. The obtained chromatograms were much cleaner, due to the absence of glucose overloading, and the data was found to drift less with MTBSTFA derivatisation than with MTBSTFA derivatisation. |
| doi_str_mv | 10.1007/s11306-011-0283-6 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_swepu</sourceid><recordid>TN_cdi_swepub_primary_oai_slubar_slu_se_42601</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1709167259</sourcerecordid><originalsourceid>FETCH-LOGICAL-c455t-ce564ed7b55526d2d874f2a345b0d404384742555b42719c3c3bae1f088502cf3</originalsourceid><addsrcrecordid>eNp1kk1v1DAQhiMEEmXhB3CzOHEJHX8nR1QoIK3UC5wt23G2qZI4eBxgD_x3HKUqUqUerBl7nhm_Hk9VvaXwgQLoS6SUg6qB0hpYw2v1rLqgUvOaNy08f_Ab9rJ6hXgHIESr4aL6-yn8CmNcpjBnEntiycki8bcpTjbHU7LL7flysogEl-BzOQ45nYmzGDpSfOviGKfBI1lSzNHHkbhzCdgZt3KYbR4wD96OJPxZQhq2i8qmCzic5tfVi96OGN7c20P14_rz96uv9fHmy7erj8faCylz7YNUInTaSSmZ6ljXaNEzy4V00AkQvBFasBJ0gmnaeu65s4H20DQSmO_5oar3uvg7LKszS9Fh09lEOxgcV2fTZgwGI5gCWvjjk_y4LmU5s_MAXvVglWnKNxghaPFaBQbaIgFUy_oi51C938uVHv1cA2YzDejDONo5xBUN1dBSpZlsC_ruEXoX1zSX5piClO_lepNHd8iniJhC_yCQgtnGwezjYApvtnEwquSw-ycVdj6F9L_w00n_AKQ-uR4</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>916011371</pqid></control><display><type>article</type><title>Development of a gas chromatography/mass spectrometry based metabolomics protocol by means of statistical experimental design</title><source>Springer Nature - Complete Springer Journals</source><creator>Danielsson, Anders P. H. ; Moritz, Thomas ; Mulder, Hindrik ; Spégel, Peter</creator><creatorcontrib>Danielsson, Anders P. H. ; Moritz, Thomas ; Mulder, Hindrik ; Spégel, Peter ; Sveriges lantbruksuniversitet</creatorcontrib><description>Metabolomics is a growing research field where new protocols are rapidly developed and new applications discovered. Common applications include biomarker discovery and elucidation of drug metabolism. However, the development of such protocols rarely includes a systematic optimization followed by validation with real samples. Here a GC/MS-based protocol using methoximation followed by silylation with
N
-
tert
-butyldimethylsilyl-
N
-methyltrifluoroacetamide (MTBSTFA) for analysis of blood plasma metabolites is thoroughly developed and optimized from derivatization to detection with statistical design of experiments (DOE). Validation was performed with blood plasma samples and proved the methodology to be efficient, rapid and reliable with a total of 51 analyses performed in 24 h, with linear responses, low detection limits and good precision. The obtained chromatograms were much cleaner, due to the absence of glucose overloading, and the data was found to drift less with MTBSTFA derivatisation than with MTBSTFA derivatisation.</description><identifier>ISSN: 1573-3882</identifier><identifier>ISSN: 1573-3890</identifier><identifier>EISSN: 1573-3890</identifier><identifier>DOI: 10.1007/s11306-011-0283-6</identifier><language>eng</language><publisher>Boston: Springer US</publisher><subject>Analytical Chemistry ; Analytisk kemi ; Biochemistry ; Biomedical and Life Sciences ; Biomedicine ; Blood ; Cell Biology ; Clinical Medicine ; Developmental Biology ; DOE ; Endocrinology and Diabetes ; Endokrinologi och diabetes ; Gas chromatography ; Klinisk medicin ; Life Sciences ; Mass spectrometry ; Medical and Health Sciences ; Medicin och hälsovetenskap ; Metabolomics ; Molecular Medicine ; MTBSTFA ; Original Article ; plasma</subject><ispartof>Metabolomics, 2012-02, Vol.8 (1), p.50-63</ispartof><rights>Springer Science+Business Media, LLC 2011</rights><rights>Springer Science+Business Media, LLC 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c455t-ce564ed7b55526d2d874f2a345b0d404384742555b42719c3c3bae1f088502cf3</citedby><cites>FETCH-LOGICAL-c455t-ce564ed7b55526d2d874f2a345b0d404384742555b42719c3c3bae1f088502cf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s11306-011-0283-6$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s11306-011-0283-6$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>230,314,776,780,881,27903,27904,41467,42536,51298</link.rule.ids><backlink>$$Uhttps://lup.lub.lu.se/record/2384365$$DView record from Swedish Publication Index$$Hfree_for_read</backlink><backlink>$$Uhttps://res.slu.se/id/publ/42601$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Danielsson, Anders P. H.</creatorcontrib><creatorcontrib>Moritz, Thomas</creatorcontrib><creatorcontrib>Mulder, Hindrik</creatorcontrib><creatorcontrib>Spégel, Peter</creatorcontrib><creatorcontrib>Sveriges lantbruksuniversitet</creatorcontrib><title>Development of a gas chromatography/mass spectrometry based metabolomics protocol by means of statistical experimental design</title><title>Metabolomics</title><addtitle>Metabolomics</addtitle><description>Metabolomics is a growing research field where new protocols are rapidly developed and new applications discovered. Common applications include biomarker discovery and elucidation of drug metabolism. However, the development of such protocols rarely includes a systematic optimization followed by validation with real samples. Here a GC/MS-based protocol using methoximation followed by silylation with
N
-
tert
-butyldimethylsilyl-
N
-methyltrifluoroacetamide (MTBSTFA) for analysis of blood plasma metabolites is thoroughly developed and optimized from derivatization to detection with statistical design of experiments (DOE). Validation was performed with blood plasma samples and proved the methodology to be efficient, rapid and reliable with a total of 51 analyses performed in 24 h, with linear responses, low detection limits and good precision. The obtained chromatograms were much cleaner, due to the absence of glucose overloading, and the data was found to drift less with MTBSTFA derivatisation than with MTBSTFA derivatisation.</description><subject>Analytical Chemistry</subject><subject>Analytisk kemi</subject><subject>Biochemistry</subject><subject>Biomedical and Life Sciences</subject><subject>Biomedicine</subject><subject>Blood</subject><subject>Cell Biology</subject><subject>Clinical Medicine</subject><subject>Developmental Biology</subject><subject>DOE</subject><subject>Endocrinology and Diabetes</subject><subject>Endokrinologi och diabetes</subject><subject>Gas chromatography</subject><subject>Klinisk medicin</subject><subject>Life Sciences</subject><subject>Mass spectrometry</subject><subject>Medical and Health Sciences</subject><subject>Medicin och hälsovetenskap</subject><subject>Metabolomics</subject><subject>Molecular Medicine</subject><subject>MTBSTFA</subject><subject>Original Article</subject><subject>plasma</subject><issn>1573-3882</issn><issn>1573-3890</issn><issn>1573-3890</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kk1v1DAQhiMEEmXhB3CzOHEJHX8nR1QoIK3UC5wt23G2qZI4eBxgD_x3HKUqUqUerBl7nhm_Hk9VvaXwgQLoS6SUg6qB0hpYw2v1rLqgUvOaNy08f_Ab9rJ6hXgHIESr4aL6-yn8CmNcpjBnEntiycki8bcpTjbHU7LL7flysogEl-BzOQ45nYmzGDpSfOviGKfBI1lSzNHHkbhzCdgZt3KYbR4wD96OJPxZQhq2i8qmCzic5tfVi96OGN7c20P14_rz96uv9fHmy7erj8faCylz7YNUInTaSSmZ6ljXaNEzy4V00AkQvBFasBJ0gmnaeu65s4H20DQSmO_5oar3uvg7LKszS9Fh09lEOxgcV2fTZgwGI5gCWvjjk_y4LmU5s_MAXvVglWnKNxghaPFaBQbaIgFUy_oi51C938uVHv1cA2YzDejDONo5xBUN1dBSpZlsC_ruEXoX1zSX5piClO_lepNHd8iniJhC_yCQgtnGwezjYApvtnEwquSw-ycVdj6F9L_w00n_AKQ-uR4</recordid><startdate>20120201</startdate><enddate>20120201</enddate><creator>Danielsson, Anders P. H.</creator><creator>Moritz, Thomas</creator><creator>Mulder, Hindrik</creator><creator>Spégel, Peter</creator><general>Springer US</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M7P</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D95</scope></search><sort><creationdate>20120201</creationdate><title>Development of a gas chromatography/mass spectrometry based metabolomics protocol by means of statistical experimental design</title><author>Danielsson, Anders P. H. ; Moritz, Thomas ; Mulder, Hindrik ; Spégel, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c455t-ce564ed7b55526d2d874f2a345b0d404384742555b42719c3c3bae1f088502cf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Analytical Chemistry</topic><topic>Analytisk kemi</topic><topic>Biochemistry</topic><topic>Biomedical and Life Sciences</topic><topic>Biomedicine</topic><topic>Blood</topic><topic>Cell Biology</topic><topic>Clinical Medicine</topic><topic>Developmental Biology</topic><topic>DOE</topic><topic>Endocrinology and Diabetes</topic><topic>Endokrinologi och diabetes</topic><topic>Gas chromatography</topic><topic>Klinisk medicin</topic><topic>Life Sciences</topic><topic>Mass spectrometry</topic><topic>Medical and Health Sciences</topic><topic>Medicin och hälsovetenskap</topic><topic>Metabolomics</topic><topic>Molecular Medicine</topic><topic>MTBSTFA</topic><topic>Original Article</topic><topic>plasma</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Danielsson, Anders P. H.</creatorcontrib><creatorcontrib>Moritz, Thomas</creatorcontrib><creatorcontrib>Mulder, Hindrik</creatorcontrib><creatorcontrib>Spégel, Peter</creatorcontrib><creatorcontrib>Sveriges lantbruksuniversitet</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Biological Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Lunds universitet</collection><jtitle>Metabolomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Danielsson, Anders P. H.</au><au>Moritz, Thomas</au><au>Mulder, Hindrik</au><au>Spégel, Peter</au><aucorp>Sveriges lantbruksuniversitet</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of a gas chromatography/mass spectrometry based metabolomics protocol by means of statistical experimental design</atitle><jtitle>Metabolomics</jtitle><stitle>Metabolomics</stitle><date>2012-02-01</date><risdate>2012</risdate><volume>8</volume><issue>1</issue><spage>50</spage><epage>63</epage><pages>50-63</pages><issn>1573-3882</issn><issn>1573-3890</issn><eissn>1573-3890</eissn><abstract>Metabolomics is a growing research field where new protocols are rapidly developed and new applications discovered. Common applications include biomarker discovery and elucidation of drug metabolism. However, the development of such protocols rarely includes a systematic optimization followed by validation with real samples. Here a GC/MS-based protocol using methoximation followed by silylation with
N
-
tert
-butyldimethylsilyl-
N
-methyltrifluoroacetamide (MTBSTFA) for analysis of blood plasma metabolites is thoroughly developed and optimized from derivatization to detection with statistical design of experiments (DOE). Validation was performed with blood plasma samples and proved the methodology to be efficient, rapid and reliable with a total of 51 analyses performed in 24 h, with linear responses, low detection limits and good precision. The obtained chromatograms were much cleaner, due to the absence of glucose overloading, and the data was found to drift less with MTBSTFA derivatisation than with MTBSTFA derivatisation.</abstract><cop>Boston</cop><pub>Springer US</pub><doi>10.1007/s11306-011-0283-6</doi><tpages>14</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1573-3882 |
| ispartof | Metabolomics, 2012-02, Vol.8 (1), p.50-63 |
| issn | 1573-3882 1573-3890 1573-3890 |
| language | eng |
| recordid | cdi_swepub_primary_oai_slubar_slu_se_42601 |
| source | Springer Nature - Complete Springer Journals |
| subjects | Analytical Chemistry Analytisk kemi Biochemistry Biomedical and Life Sciences Biomedicine Blood Cell Biology Clinical Medicine Developmental Biology DOE Endocrinology and Diabetes Endokrinologi och diabetes Gas chromatography Klinisk medicin Life Sciences Mass spectrometry Medical and Health Sciences Medicin och hälsovetenskap Metabolomics Molecular Medicine MTBSTFA Original Article plasma |
| title | Development of a gas chromatography/mass spectrometry based metabolomics protocol by means of statistical experimental design |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-21T11%3A59%3A29IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_swepu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20a%20gas%20chromatography/mass%20spectrometry%20based%20metabolomics%20protocol%20by%20means%20of%20statistical%20experimental%20design&rft.jtitle=Metabolomics&rft.au=Danielsson,%20Anders%20P.%20H.&rft.aucorp=Sveriges%20lantbruksuniversitet&rft.date=2012-02-01&rft.volume=8&rft.issue=1&rft.spage=50&rft.epage=63&rft.pages=50-63&rft.issn=1573-3882&rft.eissn=1573-3890&rft_id=info:doi/10.1007/s11306-011-0283-6&rft_dat=%3Cproquest_swepu%3E1709167259%3C/proquest_swepu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=916011371&rft_id=info:pmid/&rfr_iscdi=true |