Transcriptomic and functional analyses on a Botrytis cinerea multidrug‐resistant (MDR) strain provides new insights into the potential molecular mechanisms of MDR and fitness
Botrytis cinerea is a notorious pathogen causing pre‐ and post‐harvest spoilage in many economically important crops. Excessive application of site‐specific fungicides to control the pathogen has led to the selection of strains possessing target site alterations associated with resistance to these f...
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| Veröffentlicht in: | Molecular plant pathology 2024-09, Vol.25 (9), p.e70004-n/a |
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| creator | Sofianos, Georgios Piombo, Edoardo Dubey, Mukesh Karlsson, Magnus Karaoglanidis, George Tzelepis, Georgios |
| description | Botrytis cinerea is a notorious pathogen causing pre‐ and post‐harvest spoilage in many economically important crops. Excessive application of site‐specific fungicides to control the pathogen has led to the selection of strains possessing target site alterations associated with resistance to these fungicides and/or strains overexpressing efflux transporters associated with multidrug resistance (MDR). MDR in B. cinerea has been correlated with the overexpression of atrB and mfsM2, encoding an ATP‐binding cassette (ABC) and a major facilitator superfamily (MFS) transporter, respectively. However, it remains unknown whether other transporters may also contribute to the MDR phenotype. In the current study, the transcriptome of a B. cinerea multidrug‐resistant (MDR) field strain was analysed upon exposure to the fungicide fludioxonil, and compared to the B05.10 reference strain. The transcriptome of this field strain displayed significant differences as compared to B05.10, including genes involved in sugar membrane transport, toxin production and virulence. Among the induced genes in the field strain, even before exposure to fludioxonil, were several putatively encoding ABC and MFS transmembrane transporters. Overexpression of a highly induced MFS transporter gene in the B05.10 strain led to an increased tolerance to the fungicides fluopyram and boscalid, indicating an involvement in efflux transport of these compounds. Overall, the data from this study give insights towards better understanding the molecular mechanisms involved in MDR and fitness cost, contributing to the development of more efficient control strategies against this pathogen.
A Botrytis cinerea multidrug‐resistant field strain shows induction of major transmembrane transporter genes and involvement of an major facilitator superfamily transporter in tolerance to certain fungicides. |
| doi_str_mv | 10.1111/mpp.70004 |
| format | Article |
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A Botrytis cinerea multidrug‐resistant field strain shows induction of major transmembrane transporter genes and involvement of an major facilitator superfamily transporter in tolerance to certain fungicides.</description><identifier>ISSN: 1464-6722</identifier><identifier>ISSN: 1364-3703</identifier><identifier>EISSN: 1364-3703</identifier><identifier>DOI: 10.1111/mpp.70004</identifier><identifier>PMID: 39244735</identifier><language>eng</language><publisher>England: John Wiley & Sons, Inc</publisher><subject>Agricultural Science ; Biosynthesis ; Botrytis - drug effects ; Botrytis - genetics ; Botrytis - pathogenicity ; Botrytis cinerea ; Dioxoles - pharmacology ; Drug Resistance, Fungal - drug effects ; Drug Resistance, Fungal - genetics ; Drug Resistance, Multiple, Fungal - genetics ; Economic importance ; Efflux ; fitness ; Fludioxonil ; Fruits ; Fungal Proteins - genetics ; Fungal Proteins - metabolism ; Fungicides ; Fungicides, Industrial - pharmacology ; Gene Expression Profiling ; Gene Expression Regulation, Fungal - drug effects ; Genes ; Genetic Fitness ; Genotype & phenotype ; Glucose ; Jordbruksvetenskap ; MFS‐transporters ; Molecular modelling ; Multidrug resistance ; Mutation ; Original ; Pathogens ; Pesticides ; Phenotypes ; Proteins ; Pyrroles - pharmacology ; Spoilage ; Strain analysis ; Toxins ; Transcriptome - genetics ; Transcriptomes ; Transcriptomics ; Virulence</subject><ispartof>Molecular plant pathology, 2024-09, Vol.25 (9), p.e70004-n/a</ispartof><rights>2024 The Author(s). published by British Society for Plant Pathology and John Wiley & Sons Ltd.</rights><rights>2024 The Author(s). Molecular Plant Pathology published by British Society for Plant Pathology and John Wiley & Sons Ltd.</rights><rights>2024. This work is published under http://creativecommons.org/licenses/by/4.0/ (the "License"). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c3734-68bd84de817c706ad10eb546b6dc42861706af4ba9c7841f20f09f75bdf0ef863</cites><orcidid>0000-0001-6098-138X ; 0000-0001-7393-366X ; 0009-0002-2678-7158 ; 0000-0002-7413-2052 ; 0000-0001-6144-2185 ; 0000-0003-2830-1967</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380696/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11380696/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,552,727,780,784,864,885,1417,11562,27924,27925,45574,45575,46052,46476,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39244735$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://res.slu.se/id/publ/132534$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Sofianos, Georgios</creatorcontrib><creatorcontrib>Piombo, Edoardo</creatorcontrib><creatorcontrib>Dubey, Mukesh</creatorcontrib><creatorcontrib>Karlsson, Magnus</creatorcontrib><creatorcontrib>Karaoglanidis, George</creatorcontrib><creatorcontrib>Tzelepis, Georgios</creatorcontrib><creatorcontrib>Sveriges lantbruksuniversitet</creatorcontrib><title>Transcriptomic and functional analyses on a Botrytis cinerea multidrug‐resistant (MDR) strain provides new insights into the potential molecular mechanisms of MDR and fitness</title><title>Molecular plant pathology</title><addtitle>Mol Plant Pathol</addtitle><description>Botrytis cinerea is a notorious pathogen causing pre‐ and post‐harvest spoilage in many economically important crops. Excessive application of site‐specific fungicides to control the pathogen has led to the selection of strains possessing target site alterations associated with resistance to these fungicides and/or strains overexpressing efflux transporters associated with multidrug resistance (MDR). MDR in B. cinerea has been correlated with the overexpression of atrB and mfsM2, encoding an ATP‐binding cassette (ABC) and a major facilitator superfamily (MFS) transporter, respectively. However, it remains unknown whether other transporters may also contribute to the MDR phenotype. In the current study, the transcriptome of a B. cinerea multidrug‐resistant (MDR) field strain was analysed upon exposure to the fungicide fludioxonil, and compared to the B05.10 reference strain. The transcriptome of this field strain displayed significant differences as compared to B05.10, including genes involved in sugar membrane transport, toxin production and virulence. Among the induced genes in the field strain, even before exposure to fludioxonil, were several putatively encoding ABC and MFS transmembrane transporters. Overexpression of a highly induced MFS transporter gene in the B05.10 strain led to an increased tolerance to the fungicides fluopyram and boscalid, indicating an involvement in efflux transport of these compounds. Overall, the data from this study give insights towards better understanding the molecular mechanisms involved in MDR and fitness cost, contributing to the development of more efficient control strategies against this pathogen.
A Botrytis cinerea multidrug‐resistant field strain shows induction of major transmembrane transporter genes and involvement of an major facilitator superfamily transporter in tolerance to certain fungicides.</description><subject>Agricultural Science</subject><subject>Biosynthesis</subject><subject>Botrytis - drug effects</subject><subject>Botrytis - genetics</subject><subject>Botrytis - pathogenicity</subject><subject>Botrytis cinerea</subject><subject>Dioxoles - pharmacology</subject><subject>Drug Resistance, Fungal - drug effects</subject><subject>Drug Resistance, Fungal - genetics</subject><subject>Drug Resistance, Multiple, Fungal - genetics</subject><subject>Economic importance</subject><subject>Efflux</subject><subject>fitness</subject><subject>Fludioxonil</subject><subject>Fruits</subject><subject>Fungal Proteins - genetics</subject><subject>Fungal Proteins - metabolism</subject><subject>Fungicides</subject><subject>Fungicides, Industrial - pharmacology</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Fungal - 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Excessive application of site‐specific fungicides to control the pathogen has led to the selection of strains possessing target site alterations associated with resistance to these fungicides and/or strains overexpressing efflux transporters associated with multidrug resistance (MDR). MDR in B. cinerea has been correlated with the overexpression of atrB and mfsM2, encoding an ATP‐binding cassette (ABC) and a major facilitator superfamily (MFS) transporter, respectively. However, it remains unknown whether other transporters may also contribute to the MDR phenotype. In the current study, the transcriptome of a B. cinerea multidrug‐resistant (MDR) field strain was analysed upon exposure to the fungicide fludioxonil, and compared to the B05.10 reference strain. The transcriptome of this field strain displayed significant differences as compared to B05.10, including genes involved in sugar membrane transport, toxin production and virulence. Among the induced genes in the field strain, even before exposure to fludioxonil, were several putatively encoding ABC and MFS transmembrane transporters. Overexpression of a highly induced MFS transporter gene in the B05.10 strain led to an increased tolerance to the fungicides fluopyram and boscalid, indicating an involvement in efflux transport of these compounds. Overall, the data from this study give insights towards better understanding the molecular mechanisms involved in MDR and fitness cost, contributing to the development of more efficient control strategies against this pathogen.
A Botrytis cinerea multidrug‐resistant field strain shows induction of major transmembrane transporter genes and involvement of an major facilitator superfamily transporter in tolerance to certain fungicides.</abstract><cop>England</cop><pub>John Wiley & Sons, Inc</pub><pmid>39244735</pmid><doi>10.1111/mpp.70004</doi><tpages>13</tpages><orcidid>https://orcid.org/0000-0001-6098-138X</orcidid><orcidid>https://orcid.org/0000-0001-7393-366X</orcidid><orcidid>https://orcid.org/0009-0002-2678-7158</orcidid><orcidid>https://orcid.org/0000-0002-7413-2052</orcidid><orcidid>https://orcid.org/0000-0001-6144-2185</orcidid><orcidid>https://orcid.org/0000-0003-2830-1967</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1464-6722 |
| ispartof | Molecular plant pathology, 2024-09, Vol.25 (9), p.e70004-n/a |
| issn | 1464-6722 1364-3703 1364-3703 |
| language | eng |
| recordid | cdi_swepub_primary_oai_slubar_slu_se_132534 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; SWEPUB Freely available online; Wiley Online Library Open Access; EZB-FREE-00999 freely available EZB journals; Wiley Online Library All Journals; PubMed Central |
| subjects | Agricultural Science Biosynthesis Botrytis - drug effects Botrytis - genetics Botrytis - pathogenicity Botrytis cinerea Dioxoles - pharmacology Drug Resistance, Fungal - drug effects Drug Resistance, Fungal - genetics Drug Resistance, Multiple, Fungal - genetics Economic importance Efflux fitness Fludioxonil Fruits Fungal Proteins - genetics Fungal Proteins - metabolism Fungicides Fungicides, Industrial - pharmacology Gene Expression Profiling Gene Expression Regulation, Fungal - drug effects Genes Genetic Fitness Genotype & phenotype Glucose Jordbruksvetenskap MFS‐transporters Molecular modelling Multidrug resistance Mutation Original Pathogens Pesticides Phenotypes Proteins Pyrroles - pharmacology Spoilage Strain analysis Toxins Transcriptome - genetics Transcriptomes Transcriptomics Virulence |
| title | Transcriptomic and functional analyses on a Botrytis cinerea multidrug‐resistant (MDR) strain provides new insights into the potential molecular mechanisms of MDR and fitness |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T05%3A41%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_swepu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Transcriptomic%20and%20functional%20analyses%20on%20a%20Botrytis%20cinerea%20multidrug%E2%80%90resistant%20(MDR)%20strain%20provides%20new%20insights%20into%20the%20potential%20molecular%20mechanisms%20of%20MDR%20and%20fitness&rft.jtitle=Molecular%20plant%20pathology&rft.au=Sofianos,%20Georgios&rft.aucorp=Sveriges%20lantbruksuniversitet&rft.date=2024-09&rft.volume=25&rft.issue=9&rft.spage=e70004&rft.epage=n/a&rft.pages=e70004-n/a&rft.issn=1464-6722&rft.eissn=1364-3703&rft_id=info:doi/10.1111/mpp.70004&rft_dat=%3Cproquest_swepu%3E3110088440%3C/proquest_swepu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=3110088440&rft_id=info:pmid/39244735&rfr_iscdi=true |