Low-density colloid centrifugation removes bacteria from boar semen doses after spiking with selected species

Single-layer centrifugation (SLC) with a low-density colloid is an efficient method for removing contaminating microorganisms from boar semen while recovering most spermatozoa from the original sample. This study tested the performance of this technique, using 50-ml tubes, by spiking commercial seme...

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Veröffentlicht in:	Research in veterinary science 2023-05, Vol.158, p.215-225
Hauptverfasser:	Lacalle, E., Martínez-Martínez, S., Fernández-Alegre, E., Soriano-Úbeda, C., Morrell, J.M., Martínez-Pastor, F.
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals bacteria bacterial contamination Boar semen boars Burkholderia Burkholderia ambifaria centrifugation Centrifugation - methods Centrifugation - veterinary Chromatin Clinical Science Colloids DNA fragmentation Environmental contaminants Klinisk vetenskap Male protective effect Pseudomonas aeruginosa semen Semen - microbiology Semen Analysis - veterinary Semen Preservation - methods Semen Preservation - veterinary Silane-coated silica colloids Single-layer centrifugation species Sperm Motility sperm quality Spermatozoa Staphylococcus simulans storage time Swine veterinary medicine viability
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