Identification of a Small Translocation from 6R Possessing Stripe Rust Resistance to Wheat

Wheat stripe rust, caused by f. sp. Eriks. & E. Henn, is the most devastating fungal disease of bread wheat. Here, a wheat-rye multiple disomic substitution line, SLU126 4R (4D), 5R (5D), and 6R (7D), possessing resistance against 25 races of f. sp. , was used and crossed with Chinese Spring to...

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Veröffentlicht in:Plant disease 2023-03, Vol.107 (3), p.720-729
Hauptverfasser: Ashraf, Rimsha, Johansson, Eva, Vallenback, Pernilla, Steffenson, Brian J, Bajgain, Prabin, Rahmatov, Mahbubjon
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container_end_page 729
container_issue 3
container_start_page 720
container_title Plant disease
container_volume 107
creator Ashraf, Rimsha
Johansson, Eva
Vallenback, Pernilla
Steffenson, Brian J
Bajgain, Prabin
Rahmatov, Mahbubjon
description Wheat stripe rust, caused by f. sp. Eriks. & E. Henn, is the most devastating fungal disease of bread wheat. Here, a wheat-rye multiple disomic substitution line, SLU126 4R (4D), 5R (5D), and 6R (7D), possessing resistance against 25 races of f. sp. , was used and crossed with Chinese Spring to induce homeologous recombination to produce introgressions with a reduced rye chromosome segment. Seedling assays confirmed that the stripe rust resistance from SLU126 was retained over multiple generations. Through genotyping-by-sequencing (GBS) platforms and aligning the putative GBS-single-nucleotide polymorphism (SNPs) to the full-length annotated rye nucleotide-binding leucine-rich repeat (NLR) genes in the parental lines (CS , SLU126, CSA, and SLU820), we identified the physical position of 26, 13, and 9 NLR genes on chromosomes 6R, 4R, and 5R, respectively. The physical positions of 25 NLR genes on chromosome 6R were identified from 568,460,437 bp to 879,958,268 bp in the 6RL chromosome segment. Based on these NLR positions on the 6RL chromosome segment, the three linked SNPs (868,123,650 to 873,285,112 bp) were validated through kompetitive allele-specific PCR (KASP) assays in SLU126 and resistance plants in the family 29-N3-5. Using these KASP markers, we identified a small piece of the rye translocation (i.e., as a possible 6DS.6DL.6RL.6DL) containing the stripe resistance gene, temporary designated , within the 6RL segment. This new stripe rust resistance gene provides an additional asset for wheat improvement to mitigate yield losses caused by stripe rust.
doi_str_mv 10.1094/PDIS-07-22-1666-RE
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Eriks. &amp; E. Henn, is the most devastating fungal disease of bread wheat. Here, a wheat-rye multiple disomic substitution line, SLU126 4R (4D), 5R (5D), and 6R (7D), possessing resistance against 25 races of f. sp. , was used and crossed with Chinese Spring to induce homeologous recombination to produce introgressions with a reduced rye chromosome segment. Seedling assays confirmed that the stripe rust resistance from SLU126 was retained over multiple generations. Through genotyping-by-sequencing (GBS) platforms and aligning the putative GBS-single-nucleotide polymorphism (SNPs) to the full-length annotated rye nucleotide-binding leucine-rich repeat (NLR) genes in the parental lines (CS , SLU126, CSA, and SLU820), we identified the physical position of 26, 13, and 9 NLR genes on chromosomes 6R, 4R, and 5R, respectively. The physical positions of 25 NLR genes on chromosome 6R were identified from 568,460,437 bp to 879,958,268 bp in the 6RL chromosome segment. 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source MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; American Phytopathological Society Journal Back Issues
subjects Agricultural Science
Alleles
Basidiomycota - genetics
Chromosomes, Plant - genetics
Disease Resistance - genetics
Genetics
Genetik
Jordbruksvetenskap
Puccinia
Translocation, Genetic
Triticum - genetics
Triticum - microbiology
title Identification of a Small Translocation from 6R Possessing Stripe Rust Resistance to Wheat
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