Development and validation of a porcine artificial colonic mucus model reflecting the properties of native colonic mucus in pigs
Colonic mucus plays a key role in colonic drug absorption. Mucus permeation assays could therefore provide useful insights and support rational formulation development in the early stages of drug development. However, the collection of native colonic mucus from animal sources is labor-intensive, doe...
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| Veröffentlicht in: | European journal of pharmaceutical sciences 2023-02, Vol.181, p.106361-106361, Article 106361 |
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| container_title | European journal of pharmaceutical sciences |
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| creator | Barmpatsalou, Vicky Rodler, Agnes Jacobson, Magdalena Karlsson, Eva Marie-Louise Pedersen, Betty Lomstein Bergström, Christel Anna Sofie |
| description | Colonic mucus plays a key role in colonic drug absorption. Mucus permeation assays could therefore provide useful insights and support rational formulation development in the early stages of drug development. However, the collection of native colonic mucus from animal sources is labor-intensive, does not yield amounts that allow for routine experimentation, and raises ethical concerns. In the present study, we developed an in vitro porcine artificial colonic mucus model based on the characterization of native colonic mucus. The structural properties of the artificial colonic mucus were validated against the native secretion for their ability to capture key diffusion patterns of macromolecules in native mucus. Moreover, the artificial colonic mucus could be stored under common laboratory conditions, without compromising its barrier properties. In conclusion, the porcine artificial colonic mucus model can be considered a biorelevant way to study the diffusion behavior of drug candidates in colonic mucus. It is a cost-efficient screening tool easily incorporated into the early stages of drug development and it contributes to the implementation of the 3Rs (refinement, reduction, and replacement of animals) in the drug development process.
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| doi_str_mv | 10.1016/j.ejps.2022.106361 |
| format | Article |
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[Display omitted]</description><subject>Animals</subject><subject>Biological Assay</subject><subject>Colon</subject><subject>Diffusion</subject><subject>Drug Development</subject><subject>Mucus</subject><subject>Pathobiology</subject><subject>Patobiologi</subject><subject>Swine</subject><issn>0928-0987</issn><issn>1879-0720</issn><issn>1879-0720</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>D8T</sourceid><recordid>eNp9kU2LFDEQhoMo7rj6BzxIjh7sMUl35wO8LLt-wYIX9RqSdGXMkE7aTveIN3-6GXrdg6CnguJ9H6h6EHpOyZ4Syl8f93Ccyp4RxuqCt5w-QDsqhWqIYOQh2hHFZEOUFBfoSSlHQgiXgjxGFy3vmaS836FfN3CCmKcR0oJNGvDJxDCYJeSEs8cGT3l2IQE28xJ8cMFE7HLMKTg8rm4teMwDRDyDj-CWkA54-QZ4mvMEtQHlTEmVd4K_eiHhKRzKU_TIm1jg2d28RF_evf18_aG5_fT-4_XVbeM6wZeGWt5x76hwwsmBWAnOO26Ul521bedkR7xSIAy1UrbMdBIk96zvlBXcSt9eov3GLT9gWq2e5jCa-afOJugSV2vm89AFNGW0laoWXv2zcBO-Xuk8H_S66k51StIaf7nF6-nfVyiLHkNxEKNJkNeimej7XhKpSI2yLermXEr93D2bEn02q4_6bFafzerNbC29uOOvdoThvvJHZQ282QJQv3gKUO9xAZKDIczVjB5y-B__N9gruEI</recordid><startdate>20230201</startdate><enddate>20230201</enddate><creator>Barmpatsalou, Vicky</creator><creator>Rodler, Agnes</creator><creator>Jacobson, Magdalena</creator><creator>Karlsson, Eva Marie-Louise</creator><creator>Pedersen, Betty Lomstein</creator><creator>Bergström, Christel Anna Sofie</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>ACNBI</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>DF2</scope><scope>ZZAVC</scope><orcidid>https://orcid.org/0000-0002-8917-2612</orcidid></search><sort><creationdate>20230201</creationdate><title>Development and validation of a porcine artificial colonic mucus model reflecting the properties of native colonic mucus in pigs</title><author>Barmpatsalou, Vicky ; Rodler, Agnes ; Jacobson, Magdalena ; Karlsson, Eva Marie-Louise ; Pedersen, Betty Lomstein ; Bergström, Christel Anna Sofie</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-1b646fc17c7c8d0b8ecfc6a9f84bb34c840f99e7a1b8832a48e86f2549b76b8f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2023</creationdate><topic>Animals</topic><topic>Biological Assay</topic><topic>Colon</topic><topic>Diffusion</topic><topic>Drug Development</topic><topic>Mucus</topic><topic>Pathobiology</topic><topic>Patobiologi</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barmpatsalou, Vicky</creatorcontrib><creatorcontrib>Rodler, Agnes</creatorcontrib><creatorcontrib>Jacobson, Magdalena</creatorcontrib><creatorcontrib>Karlsson, Eva Marie-Louise</creatorcontrib><creatorcontrib>Pedersen, Betty Lomstein</creatorcontrib><creatorcontrib>Bergström, Christel Anna Sofie</creatorcontrib><creatorcontrib>Sveriges lantbruksuniversitet</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>SWEPUB Uppsala universitet full text</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SWEPUB Uppsala universitet</collection><collection>SwePub Articles full text</collection><jtitle>European journal of pharmaceutical sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barmpatsalou, Vicky</au><au>Rodler, Agnes</au><au>Jacobson, Magdalena</au><au>Karlsson, Eva Marie-Louise</au><au>Pedersen, Betty Lomstein</au><au>Bergström, Christel Anna Sofie</au><aucorp>Sveriges lantbruksuniversitet</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development and validation of a porcine artificial colonic mucus model reflecting the properties of native colonic mucus in pigs</atitle><jtitle>European journal of pharmaceutical sciences</jtitle><addtitle>Eur J Pharm Sci</addtitle><date>2023-02-01</date><risdate>2023</risdate><volume>181</volume><spage>106361</spage><epage>106361</epage><pages>106361-106361</pages><artnum>106361</artnum><issn>0928-0987</issn><issn>1879-0720</issn><eissn>1879-0720</eissn><abstract>Colonic mucus plays a key role in colonic drug absorption. Mucus permeation assays could therefore provide useful insights and support rational formulation development in the early stages of drug development. However, the collection of native colonic mucus from animal sources is labor-intensive, does not yield amounts that allow for routine experimentation, and raises ethical concerns. In the present study, we developed an in vitro porcine artificial colonic mucus model based on the characterization of native colonic mucus. The structural properties of the artificial colonic mucus were validated against the native secretion for their ability to capture key diffusion patterns of macromolecules in native mucus. Moreover, the artificial colonic mucus could be stored under common laboratory conditions, without compromising its barrier properties. In conclusion, the porcine artificial colonic mucus model can be considered a biorelevant way to study the diffusion behavior of drug candidates in colonic mucus. It is a cost-efficient screening tool easily incorporated into the early stages of drug development and it contributes to the implementation of the 3Rs (refinement, reduction, and replacement of animals) in the drug development process.
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| source | MEDLINE; DOAJ Directory of Open Access Journals; SWEPUB Freely available online; Access via ScienceDirect (Elsevier); EZB-FREE-00999 freely available EZB journals |
| subjects | Animals Biological Assay Colon Diffusion Drug Development Mucus Pathobiology Patobiologi Swine |
| title | Development and validation of a porcine artificial colonic mucus model reflecting the properties of native colonic mucus in pigs |
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