Physiological properties, composition and structural profiling of porcine gastrointestinal mucus
[Display omitted] The gastrointestinal mucus is a hydrogel that lines the luminal side of the gastrointestinal epithelium, offering barrier protection from pathogens and lubrication of the intraluminal contents. These barrier properties likewise affect nutrients and drugs that need to penetrate the...
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| Veröffentlicht in: | European journal of pharmaceutics and biopharmaceutics 2021-12, Vol.169, p.156-167 |
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| container_title | European journal of pharmaceutics and biopharmaceutics |
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| creator | Barmpatsalou, Vicky Dubbelboer, Ilse R. Rodler, Agnes Jacobson, Magdalena Karlsson, Eva Pedersen, Betty Lomstein Bergström, Christel A.S. |
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The gastrointestinal mucus is a hydrogel that lines the luminal side of the gastrointestinal epithelium, offering barrier protection from pathogens and lubrication of the intraluminal contents. These barrier properties likewise affect nutrients and drugs that need to penetrate the mucus to reach the epithelium prior to absorption.
In order to assess the potential impact of the mucus on drug absorption, we need information about the nature of the gastrointestinal mucus. Today, most of the relevant available literature is mainly derived from rodent studies. In this work, we used a larger animal species, the pig model, to characterize the mucus throughout the length of the gastrointestinal tract. This is the first report of the physiological properties (physical appearance, pH and water content), composition (protein, lipid and metabolite content) and structural profiling (rheology and gel network) of the porcine gastrointestinal mucus.
These findings allow for direct comparisons between the characteristics of mucus from various segments and can be further utilized to improve our understanding of the role of the mucus on region dependent drug absorption. Additionally, the present work is expected to contribute to the assessment of the porcine model as a preclinical species in the drug development process. |
| doi_str_mv | 10.1016/j.ejpb.2021.10.008 |
| format | Article |
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The gastrointestinal mucus is a hydrogel that lines the luminal side of the gastrointestinal epithelium, offering barrier protection from pathogens and lubrication of the intraluminal contents. These barrier properties likewise affect nutrients and drugs that need to penetrate the mucus to reach the epithelium prior to absorption.
In order to assess the potential impact of the mucus on drug absorption, we need information about the nature of the gastrointestinal mucus. Today, most of the relevant available literature is mainly derived from rodent studies. In this work, we used a larger animal species, the pig model, to characterize the mucus throughout the length of the gastrointestinal tract. This is the first report of the physiological properties (physical appearance, pH and water content), composition (protein, lipid and metabolite content) and structural profiling (rheology and gel network) of the porcine gastrointestinal mucus.
These findings allow for direct comparisons between the characteristics of mucus from various segments and can be further utilized to improve our understanding of the role of the mucus on region dependent drug absorption. Additionally, the present work is expected to contribute to the assessment of the porcine model as a preclinical species in the drug development process.</description><identifier>ISSN: 0939-6411</identifier><identifier>ISSN: 1873-3441</identifier><identifier>EISSN: 1873-3441</identifier><identifier>DOI: 10.1016/j.ejpb.2021.10.008</identifier><identifier>PMID: 34687897</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Animals ; Clinical Science ; Composition ; Cryo-SEM ; Drug Evaluation, Preclinical - methods ; Gastrointestinal ; Gastrointestinal Absorption - drug effects ; Gastrointestinal Absorption - physiology ; Gastrointestinal Tract - anatomy & histology ; Gastrointestinal Tract - physiology ; Hydrogen-Ion Concentration ; Klinisk vetenskap ; Lipidomics ; Metabolic Networks and Pathways - drug effects ; Metabolic Networks and Pathways - physiology ; Models, Animal ; Mucous Membrane - anatomy & histology ; Mucous Membrane - physiology ; Mucus ; Porcine ; Proteomics ; Rheology ; Rheology - methods ; Structure ; Swine</subject><ispartof>European journal of pharmaceutics and biopharmaceutics, 2021-12, Vol.169, p.156-167</ispartof><rights>2021</rights><rights>Copyright © 2021. Published by Elsevier B.V.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c476t-1ef3013b32367162ba542a7fc0fe54db1b15893ea2fa332862a4eb734328618c3</citedby><cites>FETCH-LOGICAL-c476t-1ef3013b32367162ba542a7fc0fe54db1b15893ea2fa332862a4eb734328618c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ejpb.2021.10.008$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>230,314,552,780,784,885,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34687897$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://urn.kb.se/resolve?urn=urn:nbn:se:uu:diva-459781$$DView record from Swedish Publication Index$$Hfree_for_read</backlink><backlink>$$Uhttps://res.slu.se/id/publ/114433$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Barmpatsalou, Vicky</creatorcontrib><creatorcontrib>Dubbelboer, Ilse R.</creatorcontrib><creatorcontrib>Rodler, Agnes</creatorcontrib><creatorcontrib>Jacobson, Magdalena</creatorcontrib><creatorcontrib>Karlsson, Eva</creatorcontrib><creatorcontrib>Pedersen, Betty Lomstein</creatorcontrib><creatorcontrib>Bergström, Christel A.S.</creatorcontrib><creatorcontrib>Sveriges lantbruksuniversitet</creatorcontrib><title>Physiological properties, composition and structural profiling of porcine gastrointestinal mucus</title><title>European journal of pharmaceutics and biopharmaceutics</title><addtitle>Eur J Pharm Biopharm</addtitle><description>[Display omitted]
The gastrointestinal mucus is a hydrogel that lines the luminal side of the gastrointestinal epithelium, offering barrier protection from pathogens and lubrication of the intraluminal contents. These barrier properties likewise affect nutrients and drugs that need to penetrate the mucus to reach the epithelium prior to absorption.
In order to assess the potential impact of the mucus on drug absorption, we need information about the nature of the gastrointestinal mucus. Today, most of the relevant available literature is mainly derived from rodent studies. In this work, we used a larger animal species, the pig model, to characterize the mucus throughout the length of the gastrointestinal tract. This is the first report of the physiological properties (physical appearance, pH and water content), composition (protein, lipid and metabolite content) and structural profiling (rheology and gel network) of the porcine gastrointestinal mucus.
These findings allow for direct comparisons between the characteristics of mucus from various segments and can be further utilized to improve our understanding of the role of the mucus on region dependent drug absorption. Additionally, the present work is expected to contribute to the assessment of the porcine model as a preclinical species in the drug development process.</description><subject>Animals</subject><subject>Clinical Science</subject><subject>Composition</subject><subject>Cryo-SEM</subject><subject>Drug Evaluation, Preclinical - methods</subject><subject>Gastrointestinal</subject><subject>Gastrointestinal Absorption - drug effects</subject><subject>Gastrointestinal Absorption - physiology</subject><subject>Gastrointestinal Tract - anatomy & histology</subject><subject>Gastrointestinal Tract - physiology</subject><subject>Hydrogen-Ion Concentration</subject><subject>Klinisk vetenskap</subject><subject>Lipidomics</subject><subject>Metabolic Networks and Pathways - drug effects</subject><subject>Metabolic Networks and Pathways - physiology</subject><subject>Models, Animal</subject><subject>Mucous Membrane - anatomy & histology</subject><subject>Mucous Membrane - physiology</subject><subject>Mucus</subject><subject>Porcine</subject><subject>Proteomics</subject><subject>Rheology</subject><subject>Rheology - methods</subject><subject>Structure</subject><subject>Swine</subject><issn>0939-6411</issn><issn>1873-3441</issn><issn>1873-3441</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>D8T</sourceid><recordid>eNp9kUFv1TAQhC0Eoq-FP8AB5cihCV7biR2JS1UoIFWCA3A1jrN5-CmJgx2D-u9xlNIbnHa1-mak2SHkBdAKKDSvTxWelq5ilEE-VJSqR-QASvKSCwGPyYG2vC0bAXBGzmM8UUqFrNVTcsZFo6Rq5YF8__zjLjo_-qOzZiyW4BcMq8N4WVg_LT661fm5MHNfxDUku6awY4Mb3Xws_FAsPlg3Y3E0mfBuXjGubs7UlGyKz8iTwYwRn9_PC_L15t2X6w_l7af3H6-vbksrZLOWgAOnwDvOeCOhYZ2pBTNysHTAWvQddFCrlqNhg-GcqYYZgZ3kYttBWX5Bqt03_sYldXoJbjLhTnvjdBxTZ8I2dEQNIATnWXD5T8Fb9-1K-3DUKWlRt1JBxl_teM7-M-WIenLR4jiaGX2KmtWq5g0VIDPKdtQGH2PA4cEbqN6a0ye9Nae35rZbbi6LXt77p27C_kHyt6oMvNkBzF_85TDnsQ5ni70LaFfde_c__z_0GqyM</recordid><startdate>20211201</startdate><enddate>20211201</enddate><creator>Barmpatsalou, Vicky</creator><creator>Dubbelboer, Ilse R.</creator><creator>Rodler, Agnes</creator><creator>Jacobson, Magdalena</creator><creator>Karlsson, Eva</creator><creator>Pedersen, Betty Lomstein</creator><creator>Bergström, Christel A.S.</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>ACNBI</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>DF2</scope><scope>ZZAVC</scope></search><sort><creationdate>20211201</creationdate><title>Physiological properties, composition and structural profiling of porcine gastrointestinal mucus</title><author>Barmpatsalou, Vicky ; Dubbelboer, Ilse R. ; Rodler, Agnes ; Jacobson, Magdalena ; Karlsson, Eva ; Pedersen, Betty Lomstein ; Bergström, Christel A.S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c476t-1ef3013b32367162ba542a7fc0fe54db1b15893ea2fa332862a4eb734328618c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Clinical Science</topic><topic>Composition</topic><topic>Cryo-SEM</topic><topic>Drug Evaluation, Preclinical - methods</topic><topic>Gastrointestinal</topic><topic>Gastrointestinal Absorption - drug effects</topic><topic>Gastrointestinal Absorption - physiology</topic><topic>Gastrointestinal Tract - anatomy & histology</topic><topic>Gastrointestinal Tract - physiology</topic><topic>Hydrogen-Ion Concentration</topic><topic>Klinisk vetenskap</topic><topic>Lipidomics</topic><topic>Metabolic Networks and Pathways - drug effects</topic><topic>Metabolic Networks and Pathways - physiology</topic><topic>Models, Animal</topic><topic>Mucous Membrane - anatomy & histology</topic><topic>Mucous Membrane - physiology</topic><topic>Mucus</topic><topic>Porcine</topic><topic>Proteomics</topic><topic>Rheology</topic><topic>Rheology - methods</topic><topic>Structure</topic><topic>Swine</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Barmpatsalou, Vicky</creatorcontrib><creatorcontrib>Dubbelboer, Ilse R.</creatorcontrib><creatorcontrib>Rodler, Agnes</creatorcontrib><creatorcontrib>Jacobson, Magdalena</creatorcontrib><creatorcontrib>Karlsson, Eva</creatorcontrib><creatorcontrib>Pedersen, Betty Lomstein</creatorcontrib><creatorcontrib>Bergström, Christel A.S.</creatorcontrib><creatorcontrib>Sveriges lantbruksuniversitet</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>SWEPUB Uppsala universitet full text</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SWEPUB Uppsala universitet</collection><collection>SwePub Articles full text</collection><jtitle>European journal of pharmaceutics and biopharmaceutics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Barmpatsalou, Vicky</au><au>Dubbelboer, Ilse R.</au><au>Rodler, Agnes</au><au>Jacobson, Magdalena</au><au>Karlsson, Eva</au><au>Pedersen, Betty Lomstein</au><au>Bergström, Christel A.S.</au><aucorp>Sveriges lantbruksuniversitet</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Physiological properties, composition and structural profiling of porcine gastrointestinal mucus</atitle><jtitle>European journal of pharmaceutics and biopharmaceutics</jtitle><addtitle>Eur J Pharm Biopharm</addtitle><date>2021-12-01</date><risdate>2021</risdate><volume>169</volume><spage>156</spage><epage>167</epage><pages>156-167</pages><issn>0939-6411</issn><issn>1873-3441</issn><eissn>1873-3441</eissn><abstract>[Display omitted]
The gastrointestinal mucus is a hydrogel that lines the luminal side of the gastrointestinal epithelium, offering barrier protection from pathogens and lubrication of the intraluminal contents. These barrier properties likewise affect nutrients and drugs that need to penetrate the mucus to reach the epithelium prior to absorption.
In order to assess the potential impact of the mucus on drug absorption, we need information about the nature of the gastrointestinal mucus. Today, most of the relevant available literature is mainly derived from rodent studies. In this work, we used a larger animal species, the pig model, to characterize the mucus throughout the length of the gastrointestinal tract. This is the first report of the physiological properties (physical appearance, pH and water content), composition (protein, lipid and metabolite content) and structural profiling (rheology and gel network) of the porcine gastrointestinal mucus.
These findings allow for direct comparisons between the characteristics of mucus from various segments and can be further utilized to improve our understanding of the role of the mucus on region dependent drug absorption. Additionally, the present work is expected to contribute to the assessment of the porcine model as a preclinical species in the drug development process.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>34687897</pmid><doi>10.1016/j.ejpb.2021.10.008</doi><tpages>12</tpages><oa>free_for_read</oa></addata></record> |
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| issn | 0939-6411 1873-3441 1873-3441 |
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| source | MEDLINE; SWEPUB Freely available online; Access via ScienceDirect (Elsevier) |
| subjects | Animals Clinical Science Composition Cryo-SEM Drug Evaluation, Preclinical - methods Gastrointestinal Gastrointestinal Absorption - drug effects Gastrointestinal Absorption - physiology Gastrointestinal Tract - anatomy & histology Gastrointestinal Tract - physiology Hydrogen-Ion Concentration Klinisk vetenskap Lipidomics Metabolic Networks and Pathways - drug effects Metabolic Networks and Pathways - physiology Models, Animal Mucous Membrane - anatomy & histology Mucous Membrane - physiology Mucus Porcine Proteomics Rheology Rheology - methods Structure Swine |
| title | Physiological properties, composition and structural profiling of porcine gastrointestinal mucus |
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