The CEP5 Peptide Promotes Abiotic Stress Tolerance, As Revealed by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis
The proteome and phosphoproteome of CEP5 overexpressing Arabidopsis seedlings have been determined. This revealed that CEP5 impacts abiotic stress-related processes. Subsequent genetic, physiological, biochemical, and pharmacological results demonstrated that CEP5-mediated signaling is relevant for...
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| Veröffentlicht in: | Molecular & cellular proteomics 2020-08, Vol.19 (8), p.1248-1262 |
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| container_title | Molecular & cellular proteomics |
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| creator | Smith, Stephanie Zhu, Shanshuo Joos, Lisa Roberts, Ianto Nikonorova, Natalia Vu, Lam Dai Stes, Elisabeth Cho, Hyunwoo Larrieu, Antoine Xuan, Wei Goodall, Benjamin van de Cotte, Brigitte Waite, Jessic Marie Rigal, Adeline Ramans Harborough, Sigurd Persiau, Geert Vanneste, Steffen Kirschner, Gwendolyn K. Vandermarliere, Elien Martens, Lennart Stahl, Yvonne Audenaert, Dominique Friml, Jirí Felix, Georg Simon, Rüdiger Bennett, Malcolm J. Bishopp, Anthony De Jaeger, Geert Ljung, Karin Kepinski, Stefan Robert, Stephanie Nemhauser, Jennifer Hwang, Ildoo Gevaert, Kris Beeckman, Tom De Smet, Ive |
| description | The proteome and phosphoproteome of CEP5 overexpressing Arabidopsis seedlings have been determined. This revealed that CEP5 impacts abiotic stress-related processes. Subsequent genetic, physiological, biochemical, and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis. Furthermore, CEP5 specifically counteracts auxin effects by stabilizing AUX/IAA transcriptional repressors.
[Display omitted]
Highlights
•Quantitative Arabidopsis (phospho)proteomes of C-TERMINALLY ENCODED PEPTIDE 5 (CEP5).•CEP5 impacts abiotic stress-related processes and counteracts auxin effects.•CEP5 signaling stabilizes AUX/IAA transcriptional repressors.•Novel peptide-dependent control mechanism that tunes auxin signaling.
Peptides derived from non-functional precursors play important roles in various developmental processes, but also in (a)biotic stress signaling. Our (phospho)proteome-wide analyses of C-TERMINALLY ENCODED PEPTIDE 5 (CEP5)-mediated changes revealed an impact on abiotic stress-related processes. Drought has a dramatic impact on plant growth, development and reproduction, and the plant hormone auxin plays a role in drought responses. Our genetic, physiological, biochemical, and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis, and that CEP5 specifically counteracts auxin effects. Specifically, we found that CEP5 signaling stabilizes AUX/IAA transcriptional repressors, suggesting the existence of a novel peptide-dependent control mechanism that tunes auxin signaling. These observations align with the recently described role of AUX/IAAs in stress tolerance and provide a novel role for CEP5 in osmotic and drought stress tolerance. |
| doi_str_mv | 10.1074/mcp.RA119.001826 |
| format | Article |
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[Display omitted]
Highlights
•Quantitative Arabidopsis (phospho)proteomes of C-TERMINALLY ENCODED PEPTIDE 5 (CEP5).•CEP5 impacts abiotic stress-related processes and counteracts auxin effects.•CEP5 signaling stabilizes AUX/IAA transcriptional repressors.•Novel peptide-dependent control mechanism that tunes auxin signaling.
Peptides derived from non-functional precursors play important roles in various developmental processes, but also in (a)biotic stress signaling. Our (phospho)proteome-wide analyses of C-TERMINALLY ENCODED PEPTIDE 5 (CEP5)-mediated changes revealed an impact on abiotic stress-related processes. Drought has a dramatic impact on plant growth, development and reproduction, and the plant hormone auxin plays a role in drought responses. Our genetic, physiological, biochemical, and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis, and that CEP5 specifically counteracts auxin effects. Specifically, we found that CEP5 signaling stabilizes AUX/IAA transcriptional repressors, suggesting the existence of a novel peptide-dependent control mechanism that tunes auxin signaling. These observations align with the recently described role of AUX/IAAs in stress tolerance and provide a novel role for CEP5 in osmotic and drought stress tolerance.</description><identifier>ISSN: 1535-9476</identifier><identifier>ISSN: 1535-9484</identifier><identifier>EISSN: 1535-9484</identifier><identifier>DOI: 10.1074/mcp.RA119.001826</identifier><identifier>PMID: 32404488</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adaptation, Physiological - genetics ; Arabidopsis ; Arabidopsis - genetics ; Arabidopsis - metabolism ; Arabidopsis - physiology ; Arabidopsis Proteins - metabolism ; Biochemistry and Molecular Biology ; Biokemi och molekylärbiologi ; Biological Transport - genetics ; developmental biology ; Droughts ; Gene Expression Regulation, Plant ; hormones ; Indoleacetic Acids - metabolism ; label-free quantification ; mass spectrometry ; Osmosis ; Peptides - metabolism ; Phosphoproteins - metabolism ; phosphoproteome ; Plant biology ; Proteasome Endopeptidase Complex - metabolism ; protein degradation ; Proteome - metabolism ; Proteomics ; Seedlings - growth & development ; signal transduction ; stress response ; Stress, Physiological - genetics ; Transcription, Genetic</subject><ispartof>Molecular & cellular proteomics, 2020-08, Vol.19 (8), p.1248-1262</ispartof><rights>2020 © 2020 Smith et al.</rights><rights>2020 Smith et al.</rights><rights>2020 © 2020 Smith et al. 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c552t-ffff2d9b242a462c618c9714e4cce8624f6d108fcbd8219474e035fab68fba9f3</citedby><cites>FETCH-LOGICAL-c552t-ffff2d9b242a462c618c9714e4cce8624f6d108fcbd8219474e035fab68fba9f3</cites><orcidid>0000-0002-0013-3239 ; 0000-0001-5078-7532 ; 0000-0002-1317-7716 ; 0000-0003-4607-8893 ; 0000-0003-2901-189X ; 0000-0002-9351-1170 ; 0000-0002-4237-0283</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8011570/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8011570/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,550,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32404488$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://res.slu.se/id/publ/106503$$DView record from Swedish Publication Index$$Hfree_for_read</backlink></links><search><creatorcontrib>Smith, Stephanie</creatorcontrib><creatorcontrib>Zhu, Shanshuo</creatorcontrib><creatorcontrib>Joos, Lisa</creatorcontrib><creatorcontrib>Roberts, Ianto</creatorcontrib><creatorcontrib>Nikonorova, Natalia</creatorcontrib><creatorcontrib>Vu, Lam Dai</creatorcontrib><creatorcontrib>Stes, Elisabeth</creatorcontrib><creatorcontrib>Cho, Hyunwoo</creatorcontrib><creatorcontrib>Larrieu, Antoine</creatorcontrib><creatorcontrib>Xuan, Wei</creatorcontrib><creatorcontrib>Goodall, Benjamin</creatorcontrib><creatorcontrib>van de Cotte, Brigitte</creatorcontrib><creatorcontrib>Waite, Jessic Marie</creatorcontrib><creatorcontrib>Rigal, Adeline</creatorcontrib><creatorcontrib>Ramans Harborough, Sigurd</creatorcontrib><creatorcontrib>Persiau, Geert</creatorcontrib><creatorcontrib>Vanneste, Steffen</creatorcontrib><creatorcontrib>Kirschner, Gwendolyn K.</creatorcontrib><creatorcontrib>Vandermarliere, Elien</creatorcontrib><creatorcontrib>Martens, Lennart</creatorcontrib><creatorcontrib>Stahl, Yvonne</creatorcontrib><creatorcontrib>Audenaert, Dominique</creatorcontrib><creatorcontrib>Friml, Jirí</creatorcontrib><creatorcontrib>Felix, Georg</creatorcontrib><creatorcontrib>Simon, Rüdiger</creatorcontrib><creatorcontrib>Bennett, Malcolm J.</creatorcontrib><creatorcontrib>Bishopp, Anthony</creatorcontrib><creatorcontrib>De Jaeger, Geert</creatorcontrib><creatorcontrib>Ljung, Karin</creatorcontrib><creatorcontrib>Kepinski, Stefan</creatorcontrib><creatorcontrib>Robert, Stephanie</creatorcontrib><creatorcontrib>Nemhauser, Jennifer</creatorcontrib><creatorcontrib>Hwang, Ildoo</creatorcontrib><creatorcontrib>Gevaert, Kris</creatorcontrib><creatorcontrib>Beeckman, Tom</creatorcontrib><creatorcontrib>De Smet, Ive</creatorcontrib><creatorcontrib>Sveriges lantbruksuniversitet</creatorcontrib><title>The CEP5 Peptide Promotes Abiotic Stress Tolerance, As Revealed by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis</title><title>Molecular & cellular proteomics</title><addtitle>Mol Cell Proteomics</addtitle><description>The proteome and phosphoproteome of CEP5 overexpressing Arabidopsis seedlings have been determined. This revealed that CEP5 impacts abiotic stress-related processes. Subsequent genetic, physiological, biochemical, and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis. Furthermore, CEP5 specifically counteracts auxin effects by stabilizing AUX/IAA transcriptional repressors.
[Display omitted]
Highlights
•Quantitative Arabidopsis (phospho)proteomes of C-TERMINALLY ENCODED PEPTIDE 5 (CEP5).•CEP5 impacts abiotic stress-related processes and counteracts auxin effects.•CEP5 signaling stabilizes AUX/IAA transcriptional repressors.•Novel peptide-dependent control mechanism that tunes auxin signaling.
Peptides derived from non-functional precursors play important roles in various developmental processes, but also in (a)biotic stress signaling. Our (phospho)proteome-wide analyses of C-TERMINALLY ENCODED PEPTIDE 5 (CEP5)-mediated changes revealed an impact on abiotic stress-related processes. Drought has a dramatic impact on plant growth, development and reproduction, and the plant hormone auxin plays a role in drought responses. Our genetic, physiological, biochemical, and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis, and that CEP5 specifically counteracts auxin effects. Specifically, we found that CEP5 signaling stabilizes AUX/IAA transcriptional repressors, suggesting the existence of a novel peptide-dependent control mechanism that tunes auxin signaling. These observations align with the recently described role of AUX/IAAs in stress tolerance and provide a novel role for CEP5 in osmotic and drought stress tolerance.</description><subject>Adaptation, Physiological - genetics</subject><subject>Arabidopsis</subject><subject>Arabidopsis - genetics</subject><subject>Arabidopsis - metabolism</subject><subject>Arabidopsis - physiology</subject><subject>Arabidopsis Proteins - metabolism</subject><subject>Biochemistry and Molecular Biology</subject><subject>Biokemi och molekylärbiologi</subject><subject>Biological Transport - genetics</subject><subject>developmental biology</subject><subject>Droughts</subject><subject>Gene Expression Regulation, Plant</subject><subject>hormones</subject><subject>Indoleacetic Acids - metabolism</subject><subject>label-free quantification</subject><subject>mass spectrometry</subject><subject>Osmosis</subject><subject>Peptides - metabolism</subject><subject>Phosphoproteins - metabolism</subject><subject>phosphoproteome</subject><subject>Plant biology</subject><subject>Proteasome Endopeptidase Complex - metabolism</subject><subject>protein degradation</subject><subject>Proteome - metabolism</subject><subject>Proteomics</subject><subject>Seedlings - growth & development</subject><subject>signal transduction</subject><subject>stress response</subject><subject>Stress, Physiological - genetics</subject><subject>Transcription, Genetic</subject><issn>1535-9476</issn><issn>1535-9484</issn><issn>1535-9484</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>D8T</sourceid><recordid>eNp1kU9v1DAQxSMEoqVw54R85NDd2o6TOByQotXSVqrEUrYSN8t_JtQoiVPb2aofg2-MtykresCXseT33oznl2XvCV4SXLGzXo_L64aQeokx4bR8kR2TIi8WNePs5eFelUfZmxB-YUwxqYrX2VFOGWaM8-Ps9_YW0Gq9KdAGxmgNoI13vYsQUKOsi1aj79FDCGjrOvBy0HCKmoCuYQeyA4PUA_o2ySHaKKPdPdojuN7qcIrkYFATIwyT3AfG1Kq5-XF22TRofTfZzipvpx7ZATVeKmvcGGx4m71qZRfg3VM9yW6-rLeri8XV1_PLVXO10EVB46JNh5paUUYlK6kuCdd1RRgwrYGXlLWlIZi3WhlOSVoCA5wXrVQlb5Ws2_wkW8654R7GSYnR2176B-GkFaGblPT7IgIIgssC58nweTYkdQ9GwxC97J75nr8M9lb8dDvBMSFFhVPAx6cA7-4mCFH0NmjoOjmAm4JIUHJMeV7vpXiWau9C8NAe2hAs9uhFQi8e0YsZfbJ8-He8g-Ev6yT4NAsgbXVnIf1PW0hAjfWgozDO_j_9D30hwUA</recordid><startdate>20200801</startdate><enddate>20200801</enddate><creator>Smith, Stephanie</creator><creator>Zhu, Shanshuo</creator><creator>Joos, Lisa</creator><creator>Roberts, Ianto</creator><creator>Nikonorova, Natalia</creator><creator>Vu, Lam Dai</creator><creator>Stes, Elisabeth</creator><creator>Cho, Hyunwoo</creator><creator>Larrieu, Antoine</creator><creator>Xuan, Wei</creator><creator>Goodall, Benjamin</creator><creator>van de Cotte, Brigitte</creator><creator>Waite, Jessic Marie</creator><creator>Rigal, Adeline</creator><creator>Ramans Harborough, Sigurd</creator><creator>Persiau, Geert</creator><creator>Vanneste, Steffen</creator><creator>Kirschner, Gwendolyn K.</creator><creator>Vandermarliere, Elien</creator><creator>Martens, Lennart</creator><creator>Stahl, Yvonne</creator><creator>Audenaert, Dominique</creator><creator>Friml, Jirí</creator><creator>Felix, Georg</creator><creator>Simon, Rüdiger</creator><creator>Bennett, Malcolm J.</creator><creator>Bishopp, Anthony</creator><creator>De Jaeger, Geert</creator><creator>Ljung, Karin</creator><creator>Kepinski, Stefan</creator><creator>Robert, Stephanie</creator><creator>Nemhauser, Jennifer</creator><creator>Hwang, Ildoo</creator><creator>Gevaert, Kris</creator><creator>Beeckman, Tom</creator><creator>De Smet, Ive</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>ADTPV</scope><scope>AOWAS</scope><scope>D8T</scope><scope>ZZAVC</scope><orcidid>https://orcid.org/0000-0002-0013-3239</orcidid><orcidid>https://orcid.org/0000-0001-5078-7532</orcidid><orcidid>https://orcid.org/0000-0002-1317-7716</orcidid><orcidid>https://orcid.org/0000-0003-4607-8893</orcidid><orcidid>https://orcid.org/0000-0003-2901-189X</orcidid><orcidid>https://orcid.org/0000-0002-9351-1170</orcidid><orcidid>https://orcid.org/0000-0002-4237-0283</orcidid></search><sort><creationdate>20200801</creationdate><title>The CEP5 Peptide Promotes Abiotic Stress Tolerance, As Revealed by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis</title><author>Smith, Stephanie ; Zhu, Shanshuo ; Joos, Lisa ; Roberts, Ianto ; Nikonorova, Natalia ; Vu, Lam Dai ; Stes, Elisabeth ; Cho, Hyunwoo ; Larrieu, Antoine ; Xuan, Wei ; Goodall, Benjamin ; van de Cotte, Brigitte ; Waite, Jessic Marie ; Rigal, Adeline ; Ramans Harborough, Sigurd ; Persiau, Geert ; Vanneste, Steffen ; Kirschner, Gwendolyn K. ; Vandermarliere, Elien ; Martens, Lennart ; Stahl, Yvonne ; Audenaert, Dominique ; Friml, Jirí ; Felix, Georg ; Simon, Rüdiger ; Bennett, Malcolm J. ; Bishopp, Anthony ; De Jaeger, Geert ; Ljung, Karin ; Kepinski, Stefan ; Robert, Stephanie ; Nemhauser, Jennifer ; Hwang, Ildoo ; Gevaert, Kris ; Beeckman, Tom ; De Smet, Ive</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c552t-ffff2d9b242a462c618c9714e4cce8624f6d108fcbd8219474e035fab68fba9f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Adaptation, Physiological - genetics</topic><topic>Arabidopsis</topic><topic>Arabidopsis - genetics</topic><topic>Arabidopsis - metabolism</topic><topic>Arabidopsis - physiology</topic><topic>Arabidopsis Proteins - metabolism</topic><topic>Biochemistry and Molecular Biology</topic><topic>Biokemi och molekylärbiologi</topic><topic>Biological Transport - genetics</topic><topic>developmental biology</topic><topic>Droughts</topic><topic>Gene Expression Regulation, Plant</topic><topic>hormones</topic><topic>Indoleacetic Acids - metabolism</topic><topic>label-free quantification</topic><topic>mass spectrometry</topic><topic>Osmosis</topic><topic>Peptides - metabolism</topic><topic>Phosphoproteins - metabolism</topic><topic>phosphoproteome</topic><topic>Plant biology</topic><topic>Proteasome Endopeptidase Complex - metabolism</topic><topic>protein degradation</topic><topic>Proteome - metabolism</topic><topic>Proteomics</topic><topic>Seedlings - growth & development</topic><topic>signal transduction</topic><topic>stress response</topic><topic>Stress, Physiological - genetics</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Smith, Stephanie</creatorcontrib><creatorcontrib>Zhu, Shanshuo</creatorcontrib><creatorcontrib>Joos, Lisa</creatorcontrib><creatorcontrib>Roberts, Ianto</creatorcontrib><creatorcontrib>Nikonorova, Natalia</creatorcontrib><creatorcontrib>Vu, Lam Dai</creatorcontrib><creatorcontrib>Stes, Elisabeth</creatorcontrib><creatorcontrib>Cho, Hyunwoo</creatorcontrib><creatorcontrib>Larrieu, Antoine</creatorcontrib><creatorcontrib>Xuan, Wei</creatorcontrib><creatorcontrib>Goodall, Benjamin</creatorcontrib><creatorcontrib>van de Cotte, Brigitte</creatorcontrib><creatorcontrib>Waite, Jessic Marie</creatorcontrib><creatorcontrib>Rigal, Adeline</creatorcontrib><creatorcontrib>Ramans Harborough, Sigurd</creatorcontrib><creatorcontrib>Persiau, Geert</creatorcontrib><creatorcontrib>Vanneste, Steffen</creatorcontrib><creatorcontrib>Kirschner, Gwendolyn K.</creatorcontrib><creatorcontrib>Vandermarliere, Elien</creatorcontrib><creatorcontrib>Martens, Lennart</creatorcontrib><creatorcontrib>Stahl, Yvonne</creatorcontrib><creatorcontrib>Audenaert, Dominique</creatorcontrib><creatorcontrib>Friml, Jirí</creatorcontrib><creatorcontrib>Felix, Georg</creatorcontrib><creatorcontrib>Simon, Rüdiger</creatorcontrib><creatorcontrib>Bennett, Malcolm J.</creatorcontrib><creatorcontrib>Bishopp, Anthony</creatorcontrib><creatorcontrib>De Jaeger, Geert</creatorcontrib><creatorcontrib>Ljung, Karin</creatorcontrib><creatorcontrib>Kepinski, Stefan</creatorcontrib><creatorcontrib>Robert, Stephanie</creatorcontrib><creatorcontrib>Nemhauser, Jennifer</creatorcontrib><creatorcontrib>Hwang, Ildoo</creatorcontrib><creatorcontrib>Gevaert, Kris</creatorcontrib><creatorcontrib>Beeckman, Tom</creatorcontrib><creatorcontrib>De Smet, Ive</creatorcontrib><creatorcontrib>Sveriges lantbruksuniversitet</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SwePub</collection><collection>SwePub Articles</collection><collection>SWEPUB Freely available online</collection><collection>SwePub Articles full text</collection><jtitle>Molecular & cellular proteomics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smith, Stephanie</au><au>Zhu, Shanshuo</au><au>Joos, Lisa</au><au>Roberts, Ianto</au><au>Nikonorova, Natalia</au><au>Vu, Lam Dai</au><au>Stes, Elisabeth</au><au>Cho, Hyunwoo</au><au>Larrieu, Antoine</au><au>Xuan, Wei</au><au>Goodall, Benjamin</au><au>van de Cotte, Brigitte</au><au>Waite, Jessic Marie</au><au>Rigal, Adeline</au><au>Ramans Harborough, Sigurd</au><au>Persiau, Geert</au><au>Vanneste, Steffen</au><au>Kirschner, Gwendolyn K.</au><au>Vandermarliere, Elien</au><au>Martens, Lennart</au><au>Stahl, Yvonne</au><au>Audenaert, Dominique</au><au>Friml, Jirí</au><au>Felix, Georg</au><au>Simon, Rüdiger</au><au>Bennett, Malcolm J.</au><au>Bishopp, Anthony</au><au>De Jaeger, Geert</au><au>Ljung, Karin</au><au>Kepinski, Stefan</au><au>Robert, Stephanie</au><au>Nemhauser, Jennifer</au><au>Hwang, Ildoo</au><au>Gevaert, Kris</au><au>Beeckman, Tom</au><au>De Smet, Ive</au><aucorp>Sveriges lantbruksuniversitet</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The CEP5 Peptide Promotes Abiotic Stress Tolerance, As Revealed by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis</atitle><jtitle>Molecular & cellular proteomics</jtitle><addtitle>Mol Cell Proteomics</addtitle><date>2020-08-01</date><risdate>2020</risdate><volume>19</volume><issue>8</issue><spage>1248</spage><epage>1262</epage><pages>1248-1262</pages><issn>1535-9476</issn><issn>1535-9484</issn><eissn>1535-9484</eissn><abstract>The proteome and phosphoproteome of CEP5 overexpressing Arabidopsis seedlings have been determined. This revealed that CEP5 impacts abiotic stress-related processes. Subsequent genetic, physiological, biochemical, and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis. Furthermore, CEP5 specifically counteracts auxin effects by stabilizing AUX/IAA transcriptional repressors.
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Highlights
•Quantitative Arabidopsis (phospho)proteomes of C-TERMINALLY ENCODED PEPTIDE 5 (CEP5).•CEP5 impacts abiotic stress-related processes and counteracts auxin effects.•CEP5 signaling stabilizes AUX/IAA transcriptional repressors.•Novel peptide-dependent control mechanism that tunes auxin signaling.
Peptides derived from non-functional precursors play important roles in various developmental processes, but also in (a)biotic stress signaling. Our (phospho)proteome-wide analyses of C-TERMINALLY ENCODED PEPTIDE 5 (CEP5)-mediated changes revealed an impact on abiotic stress-related processes. Drought has a dramatic impact on plant growth, development and reproduction, and the plant hormone auxin plays a role in drought responses. Our genetic, physiological, biochemical, and pharmacological results demonstrated that CEP5-mediated signaling is relevant for osmotic and drought stress tolerance in Arabidopsis, and that CEP5 specifically counteracts auxin effects. Specifically, we found that CEP5 signaling stabilizes AUX/IAA transcriptional repressors, suggesting the existence of a novel peptide-dependent control mechanism that tunes auxin signaling. These observations align with the recently described role of AUX/IAAs in stress tolerance and provide a novel role for CEP5 in osmotic and drought stress tolerance.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>32404488</pmid><doi>10.1074/mcp.RA119.001826</doi><tpages>15</tpages><orcidid>https://orcid.org/0000-0002-0013-3239</orcidid><orcidid>https://orcid.org/0000-0001-5078-7532</orcidid><orcidid>https://orcid.org/0000-0002-1317-7716</orcidid><orcidid>https://orcid.org/0000-0003-4607-8893</orcidid><orcidid>https://orcid.org/0000-0003-2901-189X</orcidid><orcidid>https://orcid.org/0000-0002-9351-1170</orcidid><orcidid>https://orcid.org/0000-0002-4237-0283</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1535-9476 |
| ispartof | Molecular & cellular proteomics, 2020-08, Vol.19 (8), p.1248-1262 |
| issn | 1535-9476 1535-9484 1535-9484 |
| language | eng |
| recordid | cdi_swepub_primary_oai_slubar_slu_se_106503 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection; SWEPUB Freely available online; Free Full-Text Journals in Chemistry |
| subjects | Adaptation, Physiological - genetics Arabidopsis Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis - physiology Arabidopsis Proteins - metabolism Biochemistry and Molecular Biology Biokemi och molekylärbiologi Biological Transport - genetics developmental biology Droughts Gene Expression Regulation, Plant hormones Indoleacetic Acids - metabolism label-free quantification mass spectrometry Osmosis Peptides - metabolism Phosphoproteins - metabolism phosphoproteome Plant biology Proteasome Endopeptidase Complex - metabolism protein degradation Proteome - metabolism Proteomics Seedlings - growth & development signal transduction stress response Stress, Physiological - genetics Transcription, Genetic |
| title | The CEP5 Peptide Promotes Abiotic Stress Tolerance, As Revealed by Quantitative Proteomics, and Attenuates the AUX/IAA Equilibrium in Arabidopsis |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-08T14%3A25%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_swepu&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20CEP5%20Peptide%20Promotes%20Abiotic%20Stress%20Tolerance,%20As%20Revealed%20by%20Quantitative%20Proteomics,%20and%20Attenuates%20the%20AUX/IAA%20Equilibrium%20in%20Arabidopsis&rft.jtitle=Molecular%20&%20cellular%20proteomics&rft.au=Smith,%20Stephanie&rft.aucorp=Sveriges%20lantbruksuniversitet&rft.date=2020-08-01&rft.volume=19&rft.issue=8&rft.spage=1248&rft.epage=1262&rft.pages=1248-1262&rft.issn=1535-9476&rft.eissn=1535-9484&rft_id=info:doi/10.1074/mcp.RA119.001826&rft_dat=%3Cproquest_swepu%3E2403028390%3C/proquest_swepu%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2403028390&rft_id=info:pmid/32404488&rft_els_id=S1535947620349604&rfr_iscdi=true |