Relative sensitivity of immunohistochemistry, multiple reaction monitoring mass spectrometry, in situ hybridization and PCR to detect Coxsackievirus B1 in A549 cells

Highlights • A variety of methods are used for enterovirus detection in tissue samples. • Their sensitivity vary and systematic comparisons have not been published. • RT-PCR, immunohistochemistry, in situ hybridization and proteomics were compared. • Proteomics and RT-PCR detected enteroviruses with...

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Veröffentlicht in:	Journal of clinical virology 2016-04, Vol.77, p.21-28
Hauptverfasser:	Laiho, Jutta E, Oikarinen, Maarit, Richardson, Sarah J, Frisk, Gun, Nyalwidhe, Julius, Burch, Tanya C, Morris, Margaret A, Oikarinen, Sami, Pugliese, Alberto, Dotta, Francesco, Campbell-Thompson, Martha, Nadler, Jerry, Morgan, Noel G, Hyöty, Heikki
Format:	Artikel
Sprache:	eng
Schlagworte:	A549 Cells Allergy and Immunology Coxsackievirus Coxsackievirus Infections - diagnosis Coxsackievirus Infections - virology Enterovirus B, Human - classification Enterovirus B, Human - genetics Enterovirus B, Human - metabolism Humans Immunohistochemisrty Immunohistochemistry In Situ Hybridization In Vitro Techniques Infectious Disease Polymerase Chain Reaction Proteomics Proteomics - methods RT-PCR Sensitivity Sensitivity and Specificity Tandem Mass Spectrometry
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Beschreibung
Zusammenfassung:	Highlights • A variety of methods are used for enterovirus detection in tissue samples. • Their sensitivity vary and systematic comparisons have not been published. • RT-PCR, immunohistochemistry, in situ hybridization and proteomics were compared. • Proteomics and RT-PCR detected enteroviruses with the highest sensitivity. • Proteomics open new opportunities for enterovirus detection.
ISSN:	1386-6532 1873-5967 1873-5967
DOI:	10.1016/j.jcv.2016.01.015