Nuclear apoptosis induced by isolated mitochondria: Regular Article
ABSTRACT We isolated and purified mitochondria from mouse livers and spinach leaves. When added into egg extracts of Xenopus laevis, they caused nuclei of mouse liver to undergo apoptotic changes. Chromatin condensation, margination and DNA ladder were observed. After incubating isolated mitochondri...
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| Veröffentlicht in: | Cell research 2000-09, Vol.10 (3), p.221-232 |
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| creator | JIANG, Zheng Fan ZHAO, Yun HONG, Xia ZHAI, Zhong He |
| description | ABSTRACT
We isolated and purified mitochondria from mouse livers and spinach leaves. When added into egg extracts of Xenopus laevis, they caused nuclei of mouse liver to undergo apoptotic changes. Chromatin condensation, margination and DNA ladder were observed. After incubating isolated mitochondria in some hypotonic solutions, and centrifuging these mixtures at high speed, we got mitochondrial supernatants. It was found that in the absence of cytosolic factor, the supernatant alone was able to induce apoptotic changes in nuclei. The effective components were partly of protein. DNA fragmentation was partly inhibited by caspase inhibitors AC-DEVD-CHO and AC-YVAD-CHO. Meanwhile, caspase inhibitors fully blocked chromatin condensation. Primary characterization of the nuclear endonuclease(s) induced by mitochondrial supernatants was also conducted. It was found that this endonuclease is different from endonuclease G, cytochrome c-induced nuclease, or Ca
2+
-activated endonuclease. |
| doi_str_mv | 10.1038/sj.cr.7290051 |
| format | Article |
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We isolated and purified mitochondria from mouse livers and spinach leaves. When added into egg extracts of Xenopus laevis, they caused nuclei of mouse liver to undergo apoptotic changes. Chromatin condensation, margination and DNA ladder were observed. After incubating isolated mitochondria in some hypotonic solutions, and centrifuging these mixtures at high speed, we got mitochondrial supernatants. It was found that in the absence of cytosolic factor, the supernatant alone was able to induce apoptotic changes in nuclei. The effective components were partly of protein. DNA fragmentation was partly inhibited by caspase inhibitors AC-DEVD-CHO and AC-YVAD-CHO. Meanwhile, caspase inhibitors fully blocked chromatin condensation. Primary characterization of the nuclear endonuclease(s) induced by mitochondrial supernatants was also conducted. It was found that this endonuclease is different from endonuclease G, cytochrome c-induced nuclease, or Ca
2+
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We isolated and purified mitochondria from mouse livers and spinach leaves. When added into egg extracts of Xenopus laevis, they caused nuclei of mouse liver to undergo apoptotic changes. Chromatin condensation, margination and DNA ladder were observed. After incubating isolated mitochondria in some hypotonic solutions, and centrifuging these mixtures at high speed, we got mitochondrial supernatants. It was found that in the absence of cytosolic factor, the supernatant alone was able to induce apoptotic changes in nuclei. The effective components were partly of protein. DNA fragmentation was partly inhibited by caspase inhibitors AC-DEVD-CHO and AC-YVAD-CHO. Meanwhile, caspase inhibitors fully blocked chromatin condensation. Primary characterization of the nuclear endonuclease(s) induced by mitochondrial supernatants was also conducted. It was found that this endonuclease is different from endonuclease G, cytochrome c-induced nuclease, or Ca
2+
-activated endonuclease.</description><subject>Biomedical and Life Sciences</subject><subject>Cell Biology</subject><subject>Life Sciences</subject><subject>research-article</subject><issn>1001-0602</issn><issn>1748-7838</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2000</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNqVjr0KwjAURoMoWH9G97xA403a2jiL4uTkHmIaNaEmJbcdfHsr-AJO34GPA4eQDQfGoZBb9MwkVos9QMUnJON1KfNaFnI6MgDPYQdiThaIHkBUZcUzIi6Daa1OVHex6yM6pC40g7ENvb2pw9jqfuSX66N5xtAkp1dkdtct2vVvl4SdjtfDOccuufCwSfk4pDBeioP6lin0yiT1Kyv-Fj5J3kII</recordid><startdate>20000901</startdate><enddate>20000901</enddate><creator>JIANG, Zheng Fan</creator><creator>ZHAO, Yun</creator><creator>HONG, Xia</creator><creator>ZHAI, Zhong He</creator><general>Nature Publishing Group UK</general><scope/></search><sort><creationdate>20000901</creationdate><title>Nuclear apoptosis induced by isolated mitochondria</title><author>JIANG, Zheng Fan ; ZHAO, Yun ; HONG, Xia ; ZHAI, Zhong He</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-springer_journals_10_1038_sj_cr_72900513</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2000</creationdate><topic>Biomedical and Life Sciences</topic><topic>Cell Biology</topic><topic>Life Sciences</topic><topic>research-article</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>JIANG, Zheng Fan</creatorcontrib><creatorcontrib>ZHAO, Yun</creatorcontrib><creatorcontrib>HONG, Xia</creatorcontrib><creatorcontrib>ZHAI, Zhong He</creatorcontrib><jtitle>Cell research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>JIANG, Zheng Fan</au><au>ZHAO, Yun</au><au>HONG, Xia</au><au>ZHAI, Zhong He</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Nuclear apoptosis induced by isolated mitochondria: Regular Article</atitle><jtitle>Cell research</jtitle><stitle>Cell Res</stitle><date>2000-09-01</date><risdate>2000</risdate><volume>10</volume><issue>3</issue><spage>221</spage><epage>232</epage><pages>221-232</pages><issn>1001-0602</issn><eissn>1748-7838</eissn><abstract>ABSTRACT
We isolated and purified mitochondria from mouse livers and spinach leaves. When added into egg extracts of Xenopus laevis, they caused nuclei of mouse liver to undergo apoptotic changes. Chromatin condensation, margination and DNA ladder were observed. After incubating isolated mitochondria in some hypotonic solutions, and centrifuging these mixtures at high speed, we got mitochondrial supernatants. It was found that in the absence of cytosolic factor, the supernatant alone was able to induce apoptotic changes in nuclei. The effective components were partly of protein. DNA fragmentation was partly inhibited by caspase inhibitors AC-DEVD-CHO and AC-YVAD-CHO. Meanwhile, caspase inhibitors fully blocked chromatin condensation. Primary characterization of the nuclear endonuclease(s) induced by mitochondrial supernatants was also conducted. It was found that this endonuclease is different from endonuclease G, cytochrome c-induced nuclease, or Ca
2+
-activated endonuclease.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><doi>10.1038/sj.cr.7290051</doi></addata></record> |
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| subjects | Biomedical and Life Sciences Cell Biology Life Sciences research-article |
| title | Nuclear apoptosis induced by isolated mitochondria: Regular Article |
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