Mobilization, harvesting and selection of peripheral blood stem cells in patients with autoimmune diseases undergoing autologous hematopoietic stem cell transplantation: Cell Procurement
Peripheral blood stem cells (PBSC) were mobilized in 130 patients with autoimmune diseases undergoing autologous hematopoietic stem cell transplantation using cyclophosphamide 2 g/m 2 and either granulocyte colony-stimulating factor (G-CSF) 5 mcg/kg/day (for systemic lupus erythematosus (SLE) and se...
Gespeichert in:
Veröffentlicht in: | Bone marrow transplantation (Basingstoke) 2007, Vol.39 (6), p.317-329 |
---|---|
Hauptverfasser: | , , , , , , , , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 329 |
---|---|
container_issue | 6 |
container_start_page | 317 |
container_title | Bone marrow transplantation (Basingstoke) |
container_volume | 39 |
creator | Statkute, L Verda, L Oyama, Y Traynor, A Villa, M Shook, T Clifton, R Jovanovic, B Satkus, J Loh, Y Quigley, K Yaung, K Gonda, E Krosnjar, N Spahovic, D Burt, R K |
description | Peripheral blood stem cells (PBSC) were mobilized in 130 patients with autoimmune diseases undergoing autologous hematopoietic stem cell transplantation using cyclophosphamide 2 g/m
2
and either granulocyte colony-stimulating factor (G-CSF) 5 mcg/kg/day (for systemic lupus erythematosus (SLE) and secondary progressive multiple sclerosis, SPMS) or G-CSF 10 mcg/kg/day (for relapsing remitting multiple sclerosis (RRMS), Crohn's disease (CD), systemic sclerosis (SSc), and other immune-mediated disorders). Mobilization-related mortality was 0.8% (one of 130) secondary to infection. Circulating peripheral blood (PB) CD34
+
cells/
μ
l differed significantly by disease. Collected CD34
+
cells/kg/apheresis and overall collection efficiency was significantly better using Spectra apheresis device compared to the Fenwall CS3000 instrument. Patients with SLE and RRMS achieved the lowest and the highest CD34
+
cell yields, respectively.
Ex vivo
CD34
+
cell selection employing Isolex 300iv2.5 apparatus was significantly more efficient compared to CEPRATE CS device. Circulating PB CD34
+
cells/
μ
l correlated positively with initial CD34
+
cells/kg/apheresis and enriched product CD34
+
cells/kg. Mean WBC and platelet engraftment (ANC>0.5 × 10
9
/l and platelet count >20 × 10
9
/l) occurred on days 9 and 11, respectively. Infused CD34
+
cell/kg dose showed significant direct correlation with faster white blood cell (WBC) and platelet engraftment. When adjusted for CD34
+
cell/kg dose, patients treated with a myeloablative regimen had significantly slower WBC and platelet recovery compared to non-myeloablative regimens. |
doi_str_mv | 10.1038/sj.bmt.1705579 |
format | Article |
fullrecord | <record><control><sourceid>springer</sourceid><recordid>TN_cdi_springer_journals_10_1038_sj_bmt_1705579</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>10_1038_sj_bmt_1705579</sourcerecordid><originalsourceid>FETCH-springer_journals_10_1038_sj_bmt_17055793</originalsourceid><addsrcrecordid>eNqVkLtOxDAQRS0EEuHRUs8HkF2bvHZrBKKho7ecZDZx5HgijwMSX8Rn4l2tRE01xdEcXR0hHpTcKFnstjxt2jluVCOrqtlfiEyVTZ1XRV1dikw-1bu8KOr9tbhhnqRUZSmrTPy8U2ud_TbRkn-E0YRP5Gj9AMb3wOiwOxKgAywY7DJiMA5aR5RoxBk6dI7BeliSAn1k-LJxBLNGsvO8eoTeMhpGhtX3GAY6yRN2NNDKMOJsIi1kMdruzwkxGM-LMz6ett2Jq4NxjPfneyu2ry8fz285LyEZMeiJ1uAT0krqYxDNk05B9DlI8f-PX1DBcWc</addsrcrecordid><sourcetype>Publisher</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Mobilization, harvesting and selection of peripheral blood stem cells in patients with autoimmune diseases undergoing autologous hematopoietic stem cell transplantation: Cell Procurement</title><source>Nature</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Alma/SFX Local Collection</source><creator>Statkute, L ; Verda, L ; Oyama, Y ; Traynor, A ; Villa, M ; Shook, T ; Clifton, R ; Jovanovic, B ; Satkus, J ; Loh, Y ; Quigley, K ; Yaung, K ; Gonda, E ; Krosnjar, N ; Spahovic, D ; Burt, R K</creator><creatorcontrib>Statkute, L ; Verda, L ; Oyama, Y ; Traynor, A ; Villa, M ; Shook, T ; Clifton, R ; Jovanovic, B ; Satkus, J ; Loh, Y ; Quigley, K ; Yaung, K ; Gonda, E ; Krosnjar, N ; Spahovic, D ; Burt, R K</creatorcontrib><description>Peripheral blood stem cells (PBSC) were mobilized in 130 patients with autoimmune diseases undergoing autologous hematopoietic stem cell transplantation using cyclophosphamide 2 g/m
2
and either granulocyte colony-stimulating factor (G-CSF) 5 mcg/kg/day (for systemic lupus erythematosus (SLE) and secondary progressive multiple sclerosis, SPMS) or G-CSF 10 mcg/kg/day (for relapsing remitting multiple sclerosis (RRMS), Crohn's disease (CD), systemic sclerosis (SSc), and other immune-mediated disorders). Mobilization-related mortality was 0.8% (one of 130) secondary to infection. Circulating peripheral blood (PB) CD34
+
cells/
μ
l differed significantly by disease. Collected CD34
+
cells/kg/apheresis and overall collection efficiency was significantly better using Spectra apheresis device compared to the Fenwall CS3000 instrument. Patients with SLE and RRMS achieved the lowest and the highest CD34
+
cell yields, respectively.
Ex vivo
CD34
+
cell selection employing Isolex 300iv2.5 apparatus was significantly more efficient compared to CEPRATE CS device. Circulating PB CD34
+
cells/
μ
l correlated positively with initial CD34
+
cells/kg/apheresis and enriched product CD34
+
cells/kg. Mean WBC and platelet engraftment (ANC>0.5 × 10
9
/l and platelet count >20 × 10
9
/l) occurred on days 9 and 11, respectively. Infused CD34
+
cell/kg dose showed significant direct correlation with faster white blood cell (WBC) and platelet engraftment. When adjusted for CD34
+
cell/kg dose, patients treated with a myeloablative regimen had significantly slower WBC and platelet recovery compared to non-myeloablative regimens.</description><identifier>ISSN: 0268-3369</identifier><identifier>EISSN: 1476-5365</identifier><identifier>DOI: 10.1038/sj.bmt.1705579</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Cell Biology ; Hematology ; Internal Medicine ; Medicine ; Medicine & Public Health ; original-article ; Public Health ; Stem Cells</subject><ispartof>Bone marrow transplantation (Basingstoke), 2007, Vol.39 (6), p.317-329</ispartof><rights>Springer Nature Limited 2007</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Statkute, L</creatorcontrib><creatorcontrib>Verda, L</creatorcontrib><creatorcontrib>Oyama, Y</creatorcontrib><creatorcontrib>Traynor, A</creatorcontrib><creatorcontrib>Villa, M</creatorcontrib><creatorcontrib>Shook, T</creatorcontrib><creatorcontrib>Clifton, R</creatorcontrib><creatorcontrib>Jovanovic, B</creatorcontrib><creatorcontrib>Satkus, J</creatorcontrib><creatorcontrib>Loh, Y</creatorcontrib><creatorcontrib>Quigley, K</creatorcontrib><creatorcontrib>Yaung, K</creatorcontrib><creatorcontrib>Gonda, E</creatorcontrib><creatorcontrib>Krosnjar, N</creatorcontrib><creatorcontrib>Spahovic, D</creatorcontrib><creatorcontrib>Burt, R K</creatorcontrib><title>Mobilization, harvesting and selection of peripheral blood stem cells in patients with autoimmune diseases undergoing autologous hematopoietic stem cell transplantation: Cell Procurement</title><title>Bone marrow transplantation (Basingstoke)</title><addtitle>Bone Marrow Transplant</addtitle><description>Peripheral blood stem cells (PBSC) were mobilized in 130 patients with autoimmune diseases undergoing autologous hematopoietic stem cell transplantation using cyclophosphamide 2 g/m
2
and either granulocyte colony-stimulating factor (G-CSF) 5 mcg/kg/day (for systemic lupus erythematosus (SLE) and secondary progressive multiple sclerosis, SPMS) or G-CSF 10 mcg/kg/day (for relapsing remitting multiple sclerosis (RRMS), Crohn's disease (CD), systemic sclerosis (SSc), and other immune-mediated disorders). Mobilization-related mortality was 0.8% (one of 130) secondary to infection. Circulating peripheral blood (PB) CD34
+
cells/
μ
l differed significantly by disease. Collected CD34
+
cells/kg/apheresis and overall collection efficiency was significantly better using Spectra apheresis device compared to the Fenwall CS3000 instrument. Patients with SLE and RRMS achieved the lowest and the highest CD34
+
cell yields, respectively.
Ex vivo
CD34
+
cell selection employing Isolex 300iv2.5 apparatus was significantly more efficient compared to CEPRATE CS device. Circulating PB CD34
+
cells/
μ
l correlated positively with initial CD34
+
cells/kg/apheresis and enriched product CD34
+
cells/kg. Mean WBC and platelet engraftment (ANC>0.5 × 10
9
/l and platelet count >20 × 10
9
/l) occurred on days 9 and 11, respectively. Infused CD34
+
cell/kg dose showed significant direct correlation with faster white blood cell (WBC) and platelet engraftment. When adjusted for CD34
+
cell/kg dose, patients treated with a myeloablative regimen had significantly slower WBC and platelet recovery compared to non-myeloablative regimens.</description><subject>Cell Biology</subject><subject>Hematology</subject><subject>Internal Medicine</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>original-article</subject><subject>Public Health</subject><subject>Stem Cells</subject><issn>0268-3369</issn><issn>1476-5365</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid/><recordid>eNqVkLtOxDAQRS0EEuHRUs8HkF2bvHZrBKKho7ecZDZx5HgijwMSX8Rn4l2tRE01xdEcXR0hHpTcKFnstjxt2jluVCOrqtlfiEyVTZ1XRV1dikw-1bu8KOr9tbhhnqRUZSmrTPy8U2ud_TbRkn-E0YRP5Gj9AMb3wOiwOxKgAywY7DJiMA5aR5RoxBk6dI7BeliSAn1k-LJxBLNGsvO8eoTeMhpGhtX3GAY6yRN2NNDKMOJsIi1kMdruzwkxGM-LMz6ett2Jq4NxjPfneyu2ry8fz285LyEZMeiJ1uAT0krqYxDNk05B9DlI8f-PX1DBcWc</recordid><startdate>2007</startdate><enddate>2007</enddate><creator>Statkute, L</creator><creator>Verda, L</creator><creator>Oyama, Y</creator><creator>Traynor, A</creator><creator>Villa, M</creator><creator>Shook, T</creator><creator>Clifton, R</creator><creator>Jovanovic, B</creator><creator>Satkus, J</creator><creator>Loh, Y</creator><creator>Quigley, K</creator><creator>Yaung, K</creator><creator>Gonda, E</creator><creator>Krosnjar, N</creator><creator>Spahovic, D</creator><creator>Burt, R K</creator><general>Nature Publishing Group UK</general><scope/></search><sort><creationdate>2007</creationdate><title>Mobilization, harvesting and selection of peripheral blood stem cells in patients with autoimmune diseases undergoing autologous hematopoietic stem cell transplantation</title><author>Statkute, L ; Verda, L ; Oyama, Y ; Traynor, A ; Villa, M ; Shook, T ; Clifton, R ; Jovanovic, B ; Satkus, J ; Loh, Y ; Quigley, K ; Yaung, K ; Gonda, E ; Krosnjar, N ; Spahovic, D ; Burt, R K</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-springer_journals_10_1038_sj_bmt_17055793</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Cell Biology</topic><topic>Hematology</topic><topic>Internal Medicine</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>original-article</topic><topic>Public Health</topic><topic>Stem Cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Statkute, L</creatorcontrib><creatorcontrib>Verda, L</creatorcontrib><creatorcontrib>Oyama, Y</creatorcontrib><creatorcontrib>Traynor, A</creatorcontrib><creatorcontrib>Villa, M</creatorcontrib><creatorcontrib>Shook, T</creatorcontrib><creatorcontrib>Clifton, R</creatorcontrib><creatorcontrib>Jovanovic, B</creatorcontrib><creatorcontrib>Satkus, J</creatorcontrib><creatorcontrib>Loh, Y</creatorcontrib><creatorcontrib>Quigley, K</creatorcontrib><creatorcontrib>Yaung, K</creatorcontrib><creatorcontrib>Gonda, E</creatorcontrib><creatorcontrib>Krosnjar, N</creatorcontrib><creatorcontrib>Spahovic, D</creatorcontrib><creatorcontrib>Burt, R K</creatorcontrib><jtitle>Bone marrow transplantation (Basingstoke)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Statkute, L</au><au>Verda, L</au><au>Oyama, Y</au><au>Traynor, A</au><au>Villa, M</au><au>Shook, T</au><au>Clifton, R</au><au>Jovanovic, B</au><au>Satkus, J</au><au>Loh, Y</au><au>Quigley, K</au><au>Yaung, K</au><au>Gonda, E</au><au>Krosnjar, N</au><au>Spahovic, D</au><au>Burt, R K</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mobilization, harvesting and selection of peripheral blood stem cells in patients with autoimmune diseases undergoing autologous hematopoietic stem cell transplantation: Cell Procurement</atitle><jtitle>Bone marrow transplantation (Basingstoke)</jtitle><stitle>Bone Marrow Transplant</stitle><date>2007</date><risdate>2007</risdate><volume>39</volume><issue>6</issue><spage>317</spage><epage>329</epage><pages>317-329</pages><issn>0268-3369</issn><eissn>1476-5365</eissn><abstract>Peripheral blood stem cells (PBSC) were mobilized in 130 patients with autoimmune diseases undergoing autologous hematopoietic stem cell transplantation using cyclophosphamide 2 g/m
2
and either granulocyte colony-stimulating factor (G-CSF) 5 mcg/kg/day (for systemic lupus erythematosus (SLE) and secondary progressive multiple sclerosis, SPMS) or G-CSF 10 mcg/kg/day (for relapsing remitting multiple sclerosis (RRMS), Crohn's disease (CD), systemic sclerosis (SSc), and other immune-mediated disorders). Mobilization-related mortality was 0.8% (one of 130) secondary to infection. Circulating peripheral blood (PB) CD34
+
cells/
μ
l differed significantly by disease. Collected CD34
+
cells/kg/apheresis and overall collection efficiency was significantly better using Spectra apheresis device compared to the Fenwall CS3000 instrument. Patients with SLE and RRMS achieved the lowest and the highest CD34
+
cell yields, respectively.
Ex vivo
CD34
+
cell selection employing Isolex 300iv2.5 apparatus was significantly more efficient compared to CEPRATE CS device. Circulating PB CD34
+
cells/
μ
l correlated positively with initial CD34
+
cells/kg/apheresis and enriched product CD34
+
cells/kg. Mean WBC and platelet engraftment (ANC>0.5 × 10
9
/l and platelet count >20 × 10
9
/l) occurred on days 9 and 11, respectively. Infused CD34
+
cell/kg dose showed significant direct correlation with faster white blood cell (WBC) and platelet engraftment. When adjusted for CD34
+
cell/kg dose, patients treated with a myeloablative regimen had significantly slower WBC and platelet recovery compared to non-myeloablative regimens.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><doi>10.1038/sj.bmt.1705579</doi></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0268-3369 |
ispartof | Bone marrow transplantation (Basingstoke), 2007, Vol.39 (6), p.317-329 |
issn | 0268-3369 1476-5365 |
language | eng |
recordid | cdi_springer_journals_10_1038_sj_bmt_1705579 |
source | Nature; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
subjects | Cell Biology Hematology Internal Medicine Medicine Medicine & Public Health original-article Public Health Stem Cells |
title | Mobilization, harvesting and selection of peripheral blood stem cells in patients with autoimmune diseases undergoing autologous hematopoietic stem cell transplantation: Cell Procurement |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-23T10%3A54%3A05IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-springer&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Mobilization,%20harvesting%20and%20selection%20of%20peripheral%20blood%20stem%20cells%20in%20patients%20with%20autoimmune%20diseases%20undergoing%20autologous%20hematopoietic%20stem%20cell%20transplantation:%20Cell%20Procurement&rft.jtitle=Bone%20marrow%20transplantation%20(Basingstoke)&rft.au=Statkute,%20L&rft.date=2007&rft.volume=39&rft.issue=6&rft.spage=317&rft.epage=329&rft.pages=317-329&rft.issn=0268-3369&rft.eissn=1476-5365&rft_id=info:doi/10.1038/sj.bmt.1705579&rft_dat=%3Cspringer%3E10_1038_sj_bmt_1705579%3C/springer%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |