Application of CRISPR/Cas9 Pooled Screening for a Non-immediate Readout Model
Linking phenotypes to genetic components has been an essential part of novel drug discovery, and screening methods have been widely employed to achieve such a goal. Screens can be conducted in either pooled or arrayed formats. Although arrayed screenings provide a better and cheaper alternative in s...
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| Veröffentlicht in: | Methods in molecular biology (Clifton, N.J.) N.J.), 2024-03, Vol.2849, p.87-116 |
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| container_title | Methods in molecular biology (Clifton, N.J.) |
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| creator | Terzi Çizmecioglu, Nihal |
| description | Linking phenotypes to genetic components has been an essential part of novel drug discovery, and screening methods have been widely employed to achieve such a goal. Screens can be conducted in either pooled or arrayed formats. Although arrayed screenings provide a better and cheaper alternative in small scale, the larger-scale screenings are conducted in pooled manner. With its adaptability to various models and conditions, CRISPR/Cas9 technology provides an invaluable alternative to classical and RNAi-based screening methods. Combined with high-throughput sequencing and bioinformatics, CRISPR-/Cas9-based pooled screening methods provide unbiased and robust data. In this protocol, we employed CRISPR-/Cas9-based pooled screening for a non-binary and non-immediate readout. |
| doi_str_mv | 10.1007/7651_2024_529 |
| format | Article |
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| ispartof | Methods in molecular biology (Clifton, N.J.), 2024-03, Vol.2849, p.87-116 |
| issn | 1064-3745 1940-6029 1940-6029 |
| language | eng |
| recordid | cdi_springer_books_10_1007_7651_2024_529 |
| source | Springer Books |
| subjects | Cas9 CRISPR Differentiation Neuroectoderm Pooled screening Sox1GFP |
| title | Application of CRISPR/Cas9 Pooled Screening for a Non-immediate Readout Model |
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