Three-dimensional differential interference contrast microscopy using synthetic aperture imaging
We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image f...
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| Veröffentlicht in: | Journal of Biomedical Optics 2012-02, Vol.17 (2), p.026003-026007 |
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| container_end_page | 026007 |
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| container_issue | 2 |
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| container_title | Journal of Biomedical Optics |
| container_volume | 17 |
| creator | Kim, Moonseok Choi, Youngwoon Fang-Yen, Christopher Sung, Yongjin Kim, Kwanhyung Dasari, Ramachandra R Feld, Michael S Choi, Wonshik |
| description | We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. For an aperture synthesized coherent image, we apply for the numerical post-processing and obtain a high-contrast DIC image for arbitrary shearing direction and bias retardation. In addition, we obtain images at different depths without a scanning objective lens by numerically propagating the acquired coherent images. Our method achieves high-resolution and high-contrast 3-D DIC imaging of live biological cells. The proposed method will be useful for monitoring 3-D dynamics of intracellular particles. |
| doi_str_mv | 10.1117/1.JBO.17.2.026003 |
| format | Article |
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The proposed method will be useful for monitoring 3-D dynamics of intracellular particles.</description><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Image Enhancement - instrumentation</subject><subject>image processing</subject><subject>Imaging, Three-Dimensional - instrumentation</subject><subject>interferometry</subject><subject>Lenses</subject><subject>microscopy</subject><subject>Microscopy, Phase-Contrast - instrumentation</subject><subject>Reproducibility of Results</subject><subject>Research Papers: Imaging</subject><subject>Sensitivity and Specificity</subject><subject>synthetic apertures</subject><issn>1083-3668</issn><issn>1560-2281</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpVUU1v1DAQtRAVLS0_gAvKjVPCjJ11nAsSVJQPVdoe2rNxnMmuUb6wnUr77_E2ZQUX-43mzZuneYy9RSgQsfqAxY_P2wKrghfAJYB4wS5wIyHnXOHLhEGJXEipztnrEH4BgJK1fMXOOS-lALG5YD_v954ob91AY3DTaPqsdV1HnsboUuHGSP6ptJTZaYzehJgNzvop2Gk-ZEtw4y4LhzHuKTqbmZl8XDxlbjC71LpiZ53pA715_i_Zw82X--tv-e326_frT7e5LUuMeaNMYwE2nW2VqLlA2TUdNDWCgYqXpcW25tByDtZUSmJZ2dqkI2zahmMNRlyyj6vuvDQDtZaOVns9--TDH_RknP6_M7q93k2PWggFCssk8P5ZwE-_FwpRDy5Y6nsz0rQEXUuhBJQKEhNX5vEIwVN32oKgj8Fo1CkYnQDXazBp5t2_9k4Tf5NIBL4Swuzo1E4ydzfbFBxgdXyBr3pPGMUfypSaRw</recordid><startdate>20120201</startdate><enddate>20120201</enddate><creator>Kim, Moonseok</creator><creator>Choi, Youngwoon</creator><creator>Fang-Yen, Christopher</creator><creator>Sung, Yongjin</creator><creator>Kim, Kwanhyung</creator><creator>Dasari, Ramachandra R</creator><creator>Feld, Michael S</creator><creator>Choi, Wonshik</creator><general>Society of Photo-Optical Instrumentation Engineers</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20120201</creationdate><title>Three-dimensional differential interference contrast microscopy using synthetic aperture imaging</title><author>Kim, Moonseok ; Choi, Youngwoon ; Fang-Yen, Christopher ; Sung, Yongjin ; Kim, Kwanhyung ; Dasari, Ramachandra R ; Feld, Michael S ; Choi, Wonshik</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c441t-b8abc005fcd8392316fbf0b910a07244c1d920d220ca786147c9a1115db2190a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Image Enhancement - instrumentation</topic><topic>image processing</topic><topic>Imaging, Three-Dimensional - instrumentation</topic><topic>interferometry</topic><topic>Lenses</topic><topic>microscopy</topic><topic>Microscopy, Phase-Contrast - instrumentation</topic><topic>Reproducibility of Results</topic><topic>Research Papers: Imaging</topic><topic>Sensitivity and Specificity</topic><topic>synthetic apertures</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, Moonseok</creatorcontrib><creatorcontrib>Choi, Youngwoon</creatorcontrib><creatorcontrib>Fang-Yen, Christopher</creatorcontrib><creatorcontrib>Sung, Yongjin</creatorcontrib><creatorcontrib>Kim, Kwanhyung</creatorcontrib><creatorcontrib>Dasari, Ramachandra R</creatorcontrib><creatorcontrib>Feld, Michael S</creatorcontrib><creatorcontrib>Choi, Wonshik</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Biomedical Optics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, Moonseok</au><au>Choi, Youngwoon</au><au>Fang-Yen, Christopher</au><au>Sung, Yongjin</au><au>Kim, Kwanhyung</au><au>Dasari, Ramachandra R</au><au>Feld, Michael S</au><au>Choi, Wonshik</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Three-dimensional differential interference contrast microscopy using synthetic aperture imaging</atitle><jtitle>Journal of Biomedical Optics</jtitle><addtitle>J Biomed Opt</addtitle><date>2012-02-01</date><risdate>2012</risdate><volume>17</volume><issue>2</issue><spage>026003</spage><epage>026007</epage><pages>026003-026007</pages><issn>1083-3668</issn><eissn>1560-2281</eissn><coden>JBOPFO</coden><abstract>We implement differential interference contrast (DIC) microscopy using high-speed synthetic aperture imaging that expands the passband of coherent imaging by a factor of 2.2. 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| issn | 1083-3668 1560-2281 |
| language | eng |
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| source | MEDLINE; PubMed Central; EZB Electronic Journals Library |
| subjects | Equipment Design Equipment Failure Analysis Image Enhancement - instrumentation image processing Imaging, Three-Dimensional - instrumentation interferometry Lenses microscopy Microscopy, Phase-Contrast - instrumentation Reproducibility of Results Research Papers: Imaging Sensitivity and Specificity synthetic apertures |
| title | Three-dimensional differential interference contrast microscopy using synthetic aperture imaging |
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