Capture of dengue virus type 3 using anionic polymer-coated magnetic beads
Dengue virus (DENV) is a mosquito-borne virus and can be transmitted to humans by mosquito vectors. Although surveillance of dengue virus-infected mosquitoes is the most effective way of controlling DENV infections, detection of DENVs in mosquitoes is limited by the low sensitivity of available dete...
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| Veröffentlicht in: | International journal of molecular medicine 2011-10, Vol.28 (4), p.625-628 |
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| container_title | International journal of molecular medicine |
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| creator | Sakudo, Akikazu Masrinoul, Promsin Tanaka, Yasuharu Ikuta, Kazuyoshi |
| description | Dengue virus (DENV) is a mosquito-borne virus and can be transmitted to
humans by mosquito vectors. Although surveillance of dengue virus-infected mosquitoes
is the most effective way of controlling DENV infections, detection of DENVs in
mosquitoes is limited by the low sensitivity of available detection methods. We
here report a method for capturing DENV type 3 (DENV-3) from mosquito cells using
magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic
anhydrate). The beads were incubated with cell culture medium of DENV-3-infected
mosquito cells, then separated from the supernatant by applying a magnetic field
and washed. Adsorption of DENV-3 on the beads was confirmed by reverse transcription-polymerase
chain reaction, which detected the presence of DENV-3 genomic RNA on the beads,
and Western blotting, which determined the major DENV-3 envelope protein on the
beads. Therefore, this capture method may enable an improvement in DENV-3 detection. |
| doi_str_mv | 10.3892/ijmm.2011.733 |
| format | Article |
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humans by mosquito vectors. Although surveillance of dengue virus-infected mosquitoes
is the most effective way of controlling DENV infections, detection of DENVs in
mosquitoes is limited by the low sensitivity of available detection methods. We
here report a method for capturing DENV type 3 (DENV-3) from mosquito cells using
magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic
anhydrate). The beads were incubated with cell culture medium of DENV-3-infected
mosquito cells, then separated from the supernatant by applying a magnetic field
and washed. Adsorption of DENV-3 on the beads was confirmed by reverse transcription-polymerase
chain reaction, which detected the presence of DENV-3 genomic RNA on the beads,
and Western blotting, which determined the major DENV-3 envelope protein on the
beads. Therefore, this capture method may enable an improvement in DENV-3 detection.</description><identifier>ISSN: 1107-3756</identifier><identifier>EISSN: 1791-244X</identifier><identifier>DOI: 10.3892/ijmm.2011.733</identifier><identifier>PMID: 21720701</identifier><language>eng</language><publisher>Greece: D.A. Spandidos</publisher><subject>Aedes - virology ; Animals ; Blotting, Western ; Cell Line ; Dengue Virus - isolation & purification ; Magnetics ; Polymers - chemistry ; Reverse Transcriptase Polymerase Chain Reaction</subject><ispartof>International journal of molecular medicine, 2011-10, Vol.28 (4), p.625-628</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,5571,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21720701$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sakudo, Akikazu</creatorcontrib><creatorcontrib>Masrinoul, Promsin</creatorcontrib><creatorcontrib>Tanaka, Yasuharu</creatorcontrib><creatorcontrib>Ikuta, Kazuyoshi</creatorcontrib><title>Capture of dengue virus type 3 using anionic polymer-coated magnetic beads</title><title>International journal of molecular medicine</title><addtitle>Int J Mol Med</addtitle><description>Dengue virus (DENV) is a mosquito-borne virus and can be transmitted to
humans by mosquito vectors. Although surveillance of dengue virus-infected mosquitoes
is the most effective way of controlling DENV infections, detection of DENVs in
mosquitoes is limited by the low sensitivity of available detection methods. We
here report a method for capturing DENV type 3 (DENV-3) from mosquito cells using
magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic
anhydrate). The beads were incubated with cell culture medium of DENV-3-infected
mosquito cells, then separated from the supernatant by applying a magnetic field
and washed. Adsorption of DENV-3 on the beads was confirmed by reverse transcription-polymerase
chain reaction, which detected the presence of DENV-3 genomic RNA on the beads,
and Western blotting, which determined the major DENV-3 envelope protein on the
beads. Therefore, this capture method may enable an improvement in DENV-3 detection.</description><subject>Aedes - virology</subject><subject>Animals</subject><subject>Blotting, Western</subject><subject>Cell Line</subject><subject>Dengue Virus - isolation & purification</subject><subject>Magnetics</subject><subject>Polymers - chemistry</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><issn>1107-3756</issn><issn>1791-244X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kE1Lw0AQhhdRrFaPXmXvkrozu9mPoxQ_KXhR8FY22UlJaZIlmwj990aqPT3D8PAy8zJ2A2IhrcP7ets0CxQACyPlCbsA4yBDpb5OpxmEyaTJ9YxdprQVAnPl7DmbIRgURsAFe1v6OIw98a7igdrNSPy77sfEh30kLvmY6nbDfVt3bV3y2O32DfVZ2fmBAm_8pqVh2hfkQ7piZ5XfJbr-45x9Pj1-LF-y1fvz6_JhlUUwesh8KUk6U3iEvAKLzrq80ABOuVLjdB8GqHLSnrRCm1stnQhYVIikNaGSc3Z7yI1j0VBYx75ufL9f_z81CXcHIUXfhjp06egcG0GrNP5C_gDgMlrZ</recordid><startdate>201110</startdate><enddate>201110</enddate><creator>Sakudo, Akikazu</creator><creator>Masrinoul, Promsin</creator><creator>Tanaka, Yasuharu</creator><creator>Ikuta, Kazuyoshi</creator><general>D.A. Spandidos</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>201110</creationdate><title>Capture of dengue virus type 3 using anionic polymer-coated magnetic beads</title><author>Sakudo, Akikazu ; Masrinoul, Promsin ; Tanaka, Yasuharu ; Ikuta, Kazuyoshi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p176t-ac3e397ba215f1829895b611949c624982d1f5e6ae6428586390d2bf22e66e243</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Aedes - virology</topic><topic>Animals</topic><topic>Blotting, Western</topic><topic>Cell Line</topic><topic>Dengue Virus - isolation & purification</topic><topic>Magnetics</topic><topic>Polymers - chemistry</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sakudo, Akikazu</creatorcontrib><creatorcontrib>Masrinoul, Promsin</creatorcontrib><creatorcontrib>Tanaka, Yasuharu</creatorcontrib><creatorcontrib>Ikuta, Kazuyoshi</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>International journal of molecular medicine</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sakudo, Akikazu</au><au>Masrinoul, Promsin</au><au>Tanaka, Yasuharu</au><au>Ikuta, Kazuyoshi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Capture of dengue virus type 3 using anionic polymer-coated magnetic beads</atitle><jtitle>International journal of molecular medicine</jtitle><addtitle>Int J Mol Med</addtitle><date>2011-10</date><risdate>2011</risdate><volume>28</volume><issue>4</issue><spage>625</spage><epage>628</epage><pages>625-628</pages><issn>1107-3756</issn><eissn>1791-244X</eissn><abstract>Dengue virus (DENV) is a mosquito-borne virus and can be transmitted to
humans by mosquito vectors. Although surveillance of dengue virus-infected mosquitoes
is the most effective way of controlling DENV infections, detection of DENVs in
mosquitoes is limited by the low sensitivity of available detection methods. We
here report a method for capturing DENV type 3 (DENV-3) from mosquito cells using
magnetic beads coated with an anionic polymer, poly(methyl vinyl ether-maleic
anhydrate). The beads were incubated with cell culture medium of DENV-3-infected
mosquito cells, then separated from the supernatant by applying a magnetic field
and washed. Adsorption of DENV-3 on the beads was confirmed by reverse transcription-polymerase
chain reaction, which detected the presence of DENV-3 genomic RNA on the beads,
and Western blotting, which determined the major DENV-3 envelope protein on the
beads. Therefore, this capture method may enable an improvement in DENV-3 detection.</abstract><cop>Greece</cop><pub>D.A. Spandidos</pub><pmid>21720701</pmid><doi>10.3892/ijmm.2011.733</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1107-3756 |
| ispartof | International journal of molecular medicine, 2011-10, Vol.28 (4), p.625-628 |
| issn | 1107-3756 1791-244X |
| language | eng |
| recordid | cdi_spandidos_primary_1107-3756284625628 |
| source | Spandidos Publications Journals; MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Aedes - virology Animals Blotting, Western Cell Line Dengue Virus - isolation & purification Magnetics Polymers - chemistry Reverse Transcriptase Polymerase Chain Reaction |
| title | Capture of dengue virus type 3 using anionic polymer-coated magnetic beads |
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