Secretory expression of bovine trypsinogen by Pichia pastoris utilizing a truncated alpha-factor secretory signal sequence and a modified propeptide sequence

Trypsin plays a crucial role in processing recombinant precursor insulins, especially in removing a peptide linker or a peptide spacer by cleaving the peptide after a lysine or arginine residue. To reduce the cell toxicity of the expressed trypsin and increase the secretory expression, we developed...

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Hauptverfasser:	Kusharyoto, Wien, Hariyatun, Hariyatun, Nurdiani, Dini, Putro, Eko Wahyu, Utami, Nuruliawaty, Silvia, Rozy Ayu, Patria, Fadillah Putri
Format:	Tagungsbericht
Sprache:	eng
Schlagworte:	Cassettes Cattle Cleavage Gene expression Lysine Peptides Polypeptides Residues Three dimensional models Trypsin Yeast
Online-Zugang:	Volltext
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