Development and validation of mean centering ratio spectra spectrophotometry method for binary mixture of glimepiride and metformin hydrochloride in tablet disage form

Mean centering of ratio spectra (MCR) is an analytical method of quantitative spectrophotometric analysis for binary mixture in tablet. The purpose of this study to develop spectrophotometry method using MCR to determine the levels of glimepiride (GLI) and metformin hydrochloride (MET) in tablet dosage form using methanol as solvent. Absorption spectra of each drug were recorded and divided by the corresponding concentration of divisor, and the ratio spectra were then mean centered. The mean % recoveries were found to be in 100.30% and 99.17% for GLI and MET, respectively. The method is successfully applied to analyze GLI and MET in pharmaceutical formulation with no interference from excipients as indicated by the recovery study. All validation parameters were within the acceptable range.