A lateral flow immunoassay with self-sufficient microfluidic system for enhanced detection of thyroid-stimulating hormone
We report a self-sufficient microfluidic paper-based lateral flow immunoassay device (μLFD) for highly sensitive detection of the thyroid-stimulating hormone (TSH). Fabrication of the paper microchannels involves engraving the nitrocellulose membrane with a CO2 laser to create narrow flow paths, whi...
Gespeichert in:
| Veröffentlicht in: | AIP advances 2020-12, Vol.10 (12), p.125316-125316-7 |
|---|---|
| Hauptverfasser: | , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 125316-7 |
|---|---|
| container_issue | 12 |
| container_start_page | 125316 |
| container_title | AIP advances |
| container_volume | 10 |
| creator | Bikkarolla, Santosh Kumar McNamee, Sara E. McGregor, Stuart Vance, Paul McGhee, Helen Marlow, Emma L. McLaughlin, James |
| description | We report a self-sufficient microfluidic paper-based lateral flow immunoassay device (μLFD) for highly sensitive detection of the thyroid-stimulating hormone (TSH). Fabrication of the paper microchannels involves engraving the nitrocellulose membrane with a CO2 laser to create narrow flow paths, which constrain the fluid flow over the test zone. The proposed microchannel modified devices were studied for detection of the TSH using gold nanoparticles as labels. The effect of such microchannel modified LFDs has led to an improvement in sensitivity by nine times and the limit of detection by 6.6 times due to the slow flow rate of the sample compared with the traditional LFD. In addition, the binding of gold nanoparticles over the test line is more uniform in the case of the μLFD, thus minimizing leading-edge effects, resulting in more accurate quantitative analysis. The proposed strategy offers great potential for multiplex detection of biomarkers with increased sensitivity without introducing any hydrophobic materials to the LFD. |
| doi_str_mv | 10.1063/5.0026047 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_scita</sourceid><recordid>TN_cdi_scitation_primary_10_1063_5_0026047</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_0fea4709d4d74e9db94fbadcbd2398cc</doaj_id><sourcerecordid>2470458442</sourcerecordid><originalsourceid>FETCH-LOGICAL-c428t-1935c777c6c8f91b15dd75f5babf16218c835259cb8728c6ce872e2111b46823</originalsourceid><addsrcrecordid>eNqdkU1LxDAQhosouKgH_0HAk0LXJE3a9CjiFwhevIc0mexmaZs1SZX-e7OuqGfnMsPwzPsOM0VxTvCS4Lq65kuMaY1Zc1AsKOGirCitD__Ux8VZjBucg7UEC7Yo5hvUqwRB9cj2_gO5YZhGr2JUM_pwaY0i9LaMk7VOOxgTGpwO3vaTM06jOMcEA7I-IBjXatRgkIEEOjk_Im9RWs_BO1PG5IYpG7lxhdY-DH6E0-LIqj7C2Xc-KV7v715vH8vnl4en25vnUjMqUknaiuumaXSthW1JR7gxDbe8U50lNSVCi4pT3upONFRkCnIGSgjpWC1odVI87WWNVxu5DW5QYZZeOfnV8GElVUhO9yCxBcUa3BpmGgat6VpmO2V0Z2jVCq2z1sVeaxv82wQxyY2fwpi3lzQPMi4Y2zle7ql8qBgD2B9XguXuT5LL7z9l9mrPRu2S2l3tf_C7D7-g3BpbfQJ5uaLv</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2470458442</pqid></control><display><type>article</type><title>A lateral flow immunoassay with self-sufficient microfluidic system for enhanced detection of thyroid-stimulating hormone</title><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Bikkarolla, Santosh Kumar ; McNamee, Sara E. ; McGregor, Stuart ; Vance, Paul ; McGhee, Helen ; Marlow, Emma L. ; McLaughlin, James</creator><creatorcontrib>Bikkarolla, Santosh Kumar ; McNamee, Sara E. ; McGregor, Stuart ; Vance, Paul ; McGhee, Helen ; Marlow, Emma L. ; McLaughlin, James</creatorcontrib><description>We report a self-sufficient microfluidic paper-based lateral flow immunoassay device (μLFD) for highly sensitive detection of the thyroid-stimulating hormone (TSH). Fabrication of the paper microchannels involves engraving the nitrocellulose membrane with a CO2 laser to create narrow flow paths, which constrain the fluid flow over the test zone. The proposed microchannel modified devices were studied for detection of the TSH using gold nanoparticles as labels. The effect of such microchannel modified LFDs has led to an improvement in sensitivity by nine times and the limit of detection by 6.6 times due to the slow flow rate of the sample compared with the traditional LFD. In addition, the binding of gold nanoparticles over the test line is more uniform in the case of the μLFD, thus minimizing leading-edge effects, resulting in more accurate quantitative analysis. The proposed strategy offers great potential for multiplex detection of biomarkers with increased sensitivity without introducing any hydrophobic materials to the LFD.</description><identifier>ISSN: 2158-3226</identifier><identifier>EISSN: 2158-3226</identifier><identifier>DOI: 10.1063/5.0026047</identifier><identifier>CODEN: AAIDBI</identifier><language>eng</language><publisher>Melville: American Institute of Physics</publisher><subject>Biomarkers ; Carbon dioxide ; Carbon dioxide lasers ; Cellulose esters ; Cellulose nitrate ; Edge effect ; Engraving ; Flow paths ; Flow velocity ; Fluid dynamics ; Fluid flow ; Gold ; Immunoassay ; Microchannels ; Microfluidics ; Nanoparticles ; Self sufficiency ; Sensitivity ; Thyroid gland</subject><ispartof>AIP advances, 2020-12, Vol.10 (12), p.125316-125316-7</ispartof><rights>Author(s)</rights><rights>2020 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c428t-1935c777c6c8f91b15dd75f5babf16218c835259cb8728c6ce872e2111b46823</citedby><cites>FETCH-LOGICAL-c428t-1935c777c6c8f91b15dd75f5babf16218c835259cb8728c6ce872e2111b46823</cites><orcidid>0000-0001-5715-0323 ; 0000-0001-6026-8971</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,2096,27901,27902</link.rule.ids></links><search><creatorcontrib>Bikkarolla, Santosh Kumar</creatorcontrib><creatorcontrib>McNamee, Sara E.</creatorcontrib><creatorcontrib>McGregor, Stuart</creatorcontrib><creatorcontrib>Vance, Paul</creatorcontrib><creatorcontrib>McGhee, Helen</creatorcontrib><creatorcontrib>Marlow, Emma L.</creatorcontrib><creatorcontrib>McLaughlin, James</creatorcontrib><title>A lateral flow immunoassay with self-sufficient microfluidic system for enhanced detection of thyroid-stimulating hormone</title><title>AIP advances</title><description>We report a self-sufficient microfluidic paper-based lateral flow immunoassay device (μLFD) for highly sensitive detection of the thyroid-stimulating hormone (TSH). Fabrication of the paper microchannels involves engraving the nitrocellulose membrane with a CO2 laser to create narrow flow paths, which constrain the fluid flow over the test zone. The proposed microchannel modified devices were studied for detection of the TSH using gold nanoparticles as labels. The effect of such microchannel modified LFDs has led to an improvement in sensitivity by nine times and the limit of detection by 6.6 times due to the slow flow rate of the sample compared with the traditional LFD. In addition, the binding of gold nanoparticles over the test line is more uniform in the case of the μLFD, thus minimizing leading-edge effects, resulting in more accurate quantitative analysis. The proposed strategy offers great potential for multiplex detection of biomarkers with increased sensitivity without introducing any hydrophobic materials to the LFD.</description><subject>Biomarkers</subject><subject>Carbon dioxide</subject><subject>Carbon dioxide lasers</subject><subject>Cellulose esters</subject><subject>Cellulose nitrate</subject><subject>Edge effect</subject><subject>Engraving</subject><subject>Flow paths</subject><subject>Flow velocity</subject><subject>Fluid dynamics</subject><subject>Fluid flow</subject><subject>Gold</subject><subject>Immunoassay</subject><subject>Microchannels</subject><subject>Microfluidics</subject><subject>Nanoparticles</subject><subject>Self sufficiency</subject><subject>Sensitivity</subject><subject>Thyroid gland</subject><issn>2158-3226</issn><issn>2158-3226</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNqdkU1LxDAQhosouKgH_0HAk0LXJE3a9CjiFwhevIc0mexmaZs1SZX-e7OuqGfnMsPwzPsOM0VxTvCS4Lq65kuMaY1Zc1AsKOGirCitD__Ux8VZjBucg7UEC7Yo5hvUqwRB9cj2_gO5YZhGr2JUM_pwaY0i9LaMk7VOOxgTGpwO3vaTM06jOMcEA7I-IBjXatRgkIEEOjk_Im9RWs_BO1PG5IYpG7lxhdY-DH6E0-LIqj7C2Xc-KV7v715vH8vnl4en25vnUjMqUknaiuumaXSthW1JR7gxDbe8U50lNSVCi4pT3upONFRkCnIGSgjpWC1odVI87WWNVxu5DW5QYZZeOfnV8GElVUhO9yCxBcUa3BpmGgat6VpmO2V0Z2jVCq2z1sVeaxv82wQxyY2fwpi3lzQPMi4Y2zle7ql8qBgD2B9XguXuT5LL7z9l9mrPRu2S2l3tf_C7D7-g3BpbfQJ5uaLv</recordid><startdate>20201201</startdate><enddate>20201201</enddate><creator>Bikkarolla, Santosh Kumar</creator><creator>McNamee, Sara E.</creator><creator>McGregor, Stuart</creator><creator>Vance, Paul</creator><creator>McGhee, Helen</creator><creator>Marlow, Emma L.</creator><creator>McLaughlin, James</creator><general>American Institute of Physics</general><general>AIP Publishing LLC</general><scope>AJDQP</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>H8D</scope><scope>L7M</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0001-5715-0323</orcidid><orcidid>https://orcid.org/0000-0001-6026-8971</orcidid></search><sort><creationdate>20201201</creationdate><title>A lateral flow immunoassay with self-sufficient microfluidic system for enhanced detection of thyroid-stimulating hormone</title><author>Bikkarolla, Santosh Kumar ; McNamee, Sara E. ; McGregor, Stuart ; Vance, Paul ; McGhee, Helen ; Marlow, Emma L. ; McLaughlin, James</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c428t-1935c777c6c8f91b15dd75f5babf16218c835259cb8728c6ce872e2111b46823</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Biomarkers</topic><topic>Carbon dioxide</topic><topic>Carbon dioxide lasers</topic><topic>Cellulose esters</topic><topic>Cellulose nitrate</topic><topic>Edge effect</topic><topic>Engraving</topic><topic>Flow paths</topic><topic>Flow velocity</topic><topic>Fluid dynamics</topic><topic>Fluid flow</topic><topic>Gold</topic><topic>Immunoassay</topic><topic>Microchannels</topic><topic>Microfluidics</topic><topic>Nanoparticles</topic><topic>Self sufficiency</topic><topic>Sensitivity</topic><topic>Thyroid gland</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bikkarolla, Santosh Kumar</creatorcontrib><creatorcontrib>McNamee, Sara E.</creatorcontrib><creatorcontrib>McGregor, Stuart</creatorcontrib><creatorcontrib>Vance, Paul</creatorcontrib><creatorcontrib>McGhee, Helen</creatorcontrib><creatorcontrib>Marlow, Emma L.</creatorcontrib><creatorcontrib>McLaughlin, James</creatorcontrib><collection>AIP Open Access Journals</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Aerospace Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>AIP advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bikkarolla, Santosh Kumar</au><au>McNamee, Sara E.</au><au>McGregor, Stuart</au><au>Vance, Paul</au><au>McGhee, Helen</au><au>Marlow, Emma L.</au><au>McLaughlin, James</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>A lateral flow immunoassay with self-sufficient microfluidic system for enhanced detection of thyroid-stimulating hormone</atitle><jtitle>AIP advances</jtitle><date>2020-12-01</date><risdate>2020</risdate><volume>10</volume><issue>12</issue><spage>125316</spage><epage>125316-7</epage><pages>125316-125316-7</pages><issn>2158-3226</issn><eissn>2158-3226</eissn><coden>AAIDBI</coden><abstract>We report a self-sufficient microfluidic paper-based lateral flow immunoassay device (μLFD) for highly sensitive detection of the thyroid-stimulating hormone (TSH). Fabrication of the paper microchannels involves engraving the nitrocellulose membrane with a CO2 laser to create narrow flow paths, which constrain the fluid flow over the test zone. The proposed microchannel modified devices were studied for detection of the TSH using gold nanoparticles as labels. The effect of such microchannel modified LFDs has led to an improvement in sensitivity by nine times and the limit of detection by 6.6 times due to the slow flow rate of the sample compared with the traditional LFD. In addition, the binding of gold nanoparticles over the test line is more uniform in the case of the μLFD, thus minimizing leading-edge effects, resulting in more accurate quantitative analysis. The proposed strategy offers great potential for multiplex detection of biomarkers with increased sensitivity without introducing any hydrophobic materials to the LFD.</abstract><cop>Melville</cop><pub>American Institute of Physics</pub><doi>10.1063/5.0026047</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0001-5715-0323</orcidid><orcidid>https://orcid.org/0000-0001-6026-8971</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 2158-3226 |
| ispartof | AIP advances, 2020-12, Vol.10 (12), p.125316-125316-7 |
| issn | 2158-3226 2158-3226 |
| language | eng |
| recordid | cdi_scitation_primary_10_1063_5_0026047 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Biomarkers Carbon dioxide Carbon dioxide lasers Cellulose esters Cellulose nitrate Edge effect Engraving Flow paths Flow velocity Fluid dynamics Fluid flow Gold Immunoassay Microchannels Microfluidics Nanoparticles Self sufficiency Sensitivity Thyroid gland |
| title | A lateral flow immunoassay with self-sufficient microfluidic system for enhanced detection of thyroid-stimulating hormone |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-13T23%3A34%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_scita&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=A%20lateral%20flow%20immunoassay%20with%20self-sufficient%20microfluidic%20system%20for%20enhanced%20detection%20of%20thyroid-stimulating%20hormone&rft.jtitle=AIP%20advances&rft.au=Bikkarolla,%20Santosh%20Kumar&rft.date=2020-12-01&rft.volume=10&rft.issue=12&rft.spage=125316&rft.epage=125316-7&rft.pages=125316-125316-7&rft.issn=2158-3226&rft.eissn=2158-3226&rft.coden=AAIDBI&rft_id=info:doi/10.1063/5.0026047&rft_dat=%3Cproquest_scita%3E2470458442%3C/proquest_scita%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2470458442&rft_id=info:pmid/&rft_doaj_id=oai_doaj_org_article_0fea4709d4d74e9db94fbadcbd2398cc&rfr_iscdi=true |