Multibranched gold nanoparticles coated with serum proteins fit for photothermal tumor ablation
Photothermal tumor ablation might be carried out with multibranched gold nanoparticles (MBAuNPs) having maximum absorbance (Amax) in the infrared region and functionalized with ligands that would bind them to the target tumor markers. However, in nanomedicine applications, the nanostructures must re...
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creator | Carreón-Álvarez, Clara Sánchez-García, José Luis Sanabria-Ayala, Víctor Ortiz-Frade, Luis Antonio García-Rodríguez, Mario E. Rodríguez-López, José Luis López-Revilla, Rubén |
description | Photothermal tumor ablation might be carried out with multibranched gold nanoparticles (MBAuNPs) having maximum absorbance (Amax) in the infrared region and functionalized with ligands that would bind them to the target tumor markers. However, in nanomedicine applications, the nanostructures must reach their target tissues to be effective, but the corona of serum proteins they instantaneously acquire when administered by intravenous injection may affect their activity; for this reason, we decided to analyze the effect that exposing MBAuNPs to bovine serum albumin (BSA) and human serum (HS) have on their protein corona and physical properties. The synthesized spherical Au seeds stoichiometrically generate piñata-like MBAuNPs of 8–20 peaks potentially useful for photothermal tumor ablation since they induce hyperthermia of more than 4 °C in phantom gels mimicking the skin irradiated with an 808 nm laser at 0.75 W/cm2. The calculated surface area of MBAuNPs ranges from 24 984 nm2 to 40 669 nm2, depending on the number of peaks we use for modeling the NPs. When MBAuNPs are exposed to BSA, they acquire a protein corona with an internal “hard” portion composed by one or two layers of BSA containing ∼1000–4000 molecules covalently bound to their surface, and an external “soft” portion formed by agglomerated BSA molecules linked by non-covalent bonds. Functionalization with BSA decreases the tendency of MBAuNPs to agglomerate and increases their size dispersion. MBAuNPs and MBAuNPs–BSA exposed to HS bind HS albumin and other HS proteins ranging from 25 kDa to 180 kDa that increase their hydrodynamic diameter and decrease their stability. We conclude that MBAuNPs exposed to serum albumin and HS instantaneously acquire a hard and soft protein corona that may affect prior or subsequent functionalization aiming to direct them to specific cell or tissue targets. |
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However, in nanomedicine applications, the nanostructures must reach their target tissues to be effective, but the corona of serum proteins they instantaneously acquire when administered by intravenous injection may affect their activity; for this reason, we decided to analyze the effect that exposing MBAuNPs to bovine serum albumin (BSA) and human serum (HS) have on their protein corona and physical properties. The synthesized spherical Au seeds stoichiometrically generate piñata-like MBAuNPs of 8–20 peaks potentially useful for photothermal tumor ablation since they induce hyperthermia of more than 4 °C in phantom gels mimicking the skin irradiated with an 808 nm laser at 0.75 W/cm2. The calculated surface area of MBAuNPs ranges from 24 984 nm2 to 40 669 nm2, depending on the number of peaks we use for modeling the NPs. When MBAuNPs are exposed to BSA, they acquire a protein corona with an internal “hard” portion composed by one or two layers of BSA containing ∼1000–4000 molecules covalently bound to their surface, and an external “soft” portion formed by agglomerated BSA molecules linked by non-covalent bonds. Functionalization with BSA decreases the tendency of MBAuNPs to agglomerate and increases their size dispersion. MBAuNPs and MBAuNPs–BSA exposed to HS bind HS albumin and other HS proteins ranging from 25 kDa to 180 kDa that increase their hydrodynamic diameter and decrease their stability. We conclude that MBAuNPs exposed to serum albumin and HS instantaneously acquire a hard and soft protein corona that may affect prior or subsequent functionalization aiming to direct them to specific cell or tissue targets.</description><identifier>ISSN: 2158-3226</identifier><identifier>EISSN: 2158-3226</identifier><identifier>DOI: 10.1063/5.0025368</identifier><identifier>CODEN: AAIDBI</identifier><language>eng</language><publisher>Melville: American Institute of Physics</publisher><subject>Ablation ; Covalent bonds ; Exposure ; Gels ; Gold ; Hyperthermia ; Nanoparticles ; Physical properties ; Proteins ; Serum albumin ; Serum proteins ; Tumors</subject><ispartof>AIP advances, 2020-12, Vol.10 (12), p.125030-125030-11</ispartof><rights>Author(s)</rights><rights>2020 Author(s). All article content, except where otherwise noted, is licensed under a Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-3c25703e60dee9f81598fdf527e26f3f8f7cad15432815ae9d4d015cedb5d633</citedby><cites>FETCH-LOGICAL-c393t-3c25703e60dee9f81598fdf527e26f3f8f7cad15432815ae9d4d015cedb5d633</cites><orcidid>0000-0002-2875-9022 ; 0000-0002-3216-8057 ; 0000-0003-4113-3902</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,2096,27901,27902</link.rule.ids></links><search><creatorcontrib>Carreón-Álvarez, Clara</creatorcontrib><creatorcontrib>Sánchez-García, José Luis</creatorcontrib><creatorcontrib>Sanabria-Ayala, Víctor</creatorcontrib><creatorcontrib>Ortiz-Frade, Luis Antonio</creatorcontrib><creatorcontrib>García-Rodríguez, Mario E.</creatorcontrib><creatorcontrib>Rodríguez-López, José Luis</creatorcontrib><creatorcontrib>López-Revilla, Rubén</creatorcontrib><title>Multibranched gold nanoparticles coated with serum proteins fit for photothermal tumor ablation</title><title>AIP advances</title><description>Photothermal tumor ablation might be carried out with multibranched gold nanoparticles (MBAuNPs) having maximum absorbance (Amax) in the infrared region and functionalized with ligands that would bind them to the target tumor markers. However, in nanomedicine applications, the nanostructures must reach their target tissues to be effective, but the corona of serum proteins they instantaneously acquire when administered by intravenous injection may affect their activity; for this reason, we decided to analyze the effect that exposing MBAuNPs to bovine serum albumin (BSA) and human serum (HS) have on their protein corona and physical properties. The synthesized spherical Au seeds stoichiometrically generate piñata-like MBAuNPs of 8–20 peaks potentially useful for photothermal tumor ablation since they induce hyperthermia of more than 4 °C in phantom gels mimicking the skin irradiated with an 808 nm laser at 0.75 W/cm2. The calculated surface area of MBAuNPs ranges from 24 984 nm2 to 40 669 nm2, depending on the number of peaks we use for modeling the NPs. When MBAuNPs are exposed to BSA, they acquire a protein corona with an internal “hard” portion composed by one or two layers of BSA containing ∼1000–4000 molecules covalently bound to their surface, and an external “soft” portion formed by agglomerated BSA molecules linked by non-covalent bonds. Functionalization with BSA decreases the tendency of MBAuNPs to agglomerate and increases their size dispersion. MBAuNPs and MBAuNPs–BSA exposed to HS bind HS albumin and other HS proteins ranging from 25 kDa to 180 kDa that increase their hydrodynamic diameter and decrease their stability. We conclude that MBAuNPs exposed to serum albumin and HS instantaneously acquire a hard and soft protein corona that may affect prior or subsequent functionalization aiming to direct them to specific cell or tissue targets.</description><subject>Ablation</subject><subject>Covalent bonds</subject><subject>Exposure</subject><subject>Gels</subject><subject>Gold</subject><subject>Hyperthermia</subject><subject>Nanoparticles</subject><subject>Physical properties</subject><subject>Proteins</subject><subject>Serum albumin</subject><subject>Serum proteins</subject><subject>Tumors</subject><issn>2158-3226</issn><issn>2158-3226</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>DOA</sourceid><recordid>eNp9kUtLAzEQxxdRsNQe_AYBTwpb89hks0cpPgoVL72HNI9uynZTkyzitzftFvFkLsnM_OY_M5miuEVwjiAjj3QOIaaE8YtighHlJcGYXf55XxezGHcwn6pBkFeTQrwPXXKbIHvVGg22vtOgl70_yJCc6kwEysuUI18utSCaMOzBIfhkXB-BdQlYH8Ch9cmn1oS97EAa9tklN51Mzvc3xZWVXTSz8z0t1i_P68Vbufp4XS6eVqUiDUklUZjWkBgGtTGN5Yg23GpLcW0ws8RyWyupEa0IzjFpGl1piKgyekM1I2RaLEdZ7eVOHILby_AtvHTi5PBhK84DCVphlLM4U6ipasJlTRliSFcwW0TzrHU3auU5PwcTk9j5IfS5e4GPCYzVDc7U_Uip4GMMxv5WRVActyGoOG8jsw8jG5VLp2_5B_4BsO2JIw</recordid><startdate>20201201</startdate><enddate>20201201</enddate><creator>Carreón-Álvarez, Clara</creator><creator>Sánchez-García, José Luis</creator><creator>Sanabria-Ayala, Víctor</creator><creator>Ortiz-Frade, Luis Antonio</creator><creator>García-Rodríguez, Mario E.</creator><creator>Rodríguez-López, José Luis</creator><creator>López-Revilla, Rubén</creator><general>American Institute of Physics</general><general>AIP Publishing LLC</general><scope>AJDQP</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>H8D</scope><scope>L7M</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-2875-9022</orcidid><orcidid>https://orcid.org/0000-0002-3216-8057</orcidid><orcidid>https://orcid.org/0000-0003-4113-3902</orcidid></search><sort><creationdate>20201201</creationdate><title>Multibranched gold nanoparticles coated with serum proteins fit for photothermal tumor ablation</title><author>Carreón-Álvarez, Clara ; Sánchez-García, José Luis ; Sanabria-Ayala, Víctor ; Ortiz-Frade, Luis Antonio ; García-Rodríguez, Mario E. ; Rodríguez-López, José Luis ; López-Revilla, Rubén</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-3c25703e60dee9f81598fdf527e26f3f8f7cad15432815ae9d4d015cedb5d633</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Ablation</topic><topic>Covalent bonds</topic><topic>Exposure</topic><topic>Gels</topic><topic>Gold</topic><topic>Hyperthermia</topic><topic>Nanoparticles</topic><topic>Physical properties</topic><topic>Proteins</topic><topic>Serum albumin</topic><topic>Serum proteins</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carreón-Álvarez, Clara</creatorcontrib><creatorcontrib>Sánchez-García, José Luis</creatorcontrib><creatorcontrib>Sanabria-Ayala, Víctor</creatorcontrib><creatorcontrib>Ortiz-Frade, Luis Antonio</creatorcontrib><creatorcontrib>García-Rodríguez, Mario E.</creatorcontrib><creatorcontrib>Rodríguez-López, José Luis</creatorcontrib><creatorcontrib>López-Revilla, Rubén</creatorcontrib><collection>AIP Open Access Journals</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Aerospace Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>AIP advances</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carreón-Álvarez, Clara</au><au>Sánchez-García, José Luis</au><au>Sanabria-Ayala, Víctor</au><au>Ortiz-Frade, Luis Antonio</au><au>García-Rodríguez, Mario E.</au><au>Rodríguez-López, José Luis</au><au>López-Revilla, Rubén</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Multibranched gold nanoparticles coated with serum proteins fit for photothermal tumor ablation</atitle><jtitle>AIP advances</jtitle><date>2020-12-01</date><risdate>2020</risdate><volume>10</volume><issue>12</issue><spage>125030</spage><epage>125030-11</epage><pages>125030-125030-11</pages><issn>2158-3226</issn><eissn>2158-3226</eissn><coden>AAIDBI</coden><abstract>Photothermal tumor ablation might be carried out with multibranched gold nanoparticles (MBAuNPs) having maximum absorbance (Amax) in the infrared region and functionalized with ligands that would bind them to the target tumor markers. However, in nanomedicine applications, the nanostructures must reach their target tissues to be effective, but the corona of serum proteins they instantaneously acquire when administered by intravenous injection may affect their activity; for this reason, we decided to analyze the effect that exposing MBAuNPs to bovine serum albumin (BSA) and human serum (HS) have on their protein corona and physical properties. The synthesized spherical Au seeds stoichiometrically generate piñata-like MBAuNPs of 8–20 peaks potentially useful for photothermal tumor ablation since they induce hyperthermia of more than 4 °C in phantom gels mimicking the skin irradiated with an 808 nm laser at 0.75 W/cm2. The calculated surface area of MBAuNPs ranges from 24 984 nm2 to 40 669 nm2, depending on the number of peaks we use for modeling the NPs. When MBAuNPs are exposed to BSA, they acquire a protein corona with an internal “hard” portion composed by one or two layers of BSA containing ∼1000–4000 molecules covalently bound to their surface, and an external “soft” portion formed by agglomerated BSA molecules linked by non-covalent bonds. Functionalization with BSA decreases the tendency of MBAuNPs to agglomerate and increases their size dispersion. MBAuNPs and MBAuNPs–BSA exposed to HS bind HS albumin and other HS proteins ranging from 25 kDa to 180 kDa that increase their hydrodynamic diameter and decrease their stability. We conclude that MBAuNPs exposed to serum albumin and HS instantaneously acquire a hard and soft protein corona that may affect prior or subsequent functionalization aiming to direct them to specific cell or tissue targets.</abstract><cop>Melville</cop><pub>American Institute of Physics</pub><doi>10.1063/5.0025368</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-2875-9022</orcidid><orcidid>https://orcid.org/0000-0002-3216-8057</orcidid><orcidid>https://orcid.org/0000-0003-4113-3902</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Ablation Covalent bonds Exposure Gels Gold Hyperthermia Nanoparticles Physical properties Proteins Serum albumin Serum proteins Tumors |
title | Multibranched gold nanoparticles coated with serum proteins fit for photothermal tumor ablation |
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