Detection of HLA-B 13:01 gene among Dapsone hypersensitivity patients of leprosy in Papua Ethnics group using sequence based typing and qPCR rapid detection

Dapsone Hypersensitive Syndrome (DHS) is the rare case of immunogenic disease that cause physiological damages even death case. However, in Papua Ethnic group this disease was frequently reported among leprosy patients since Dapsone is one of the main drugs in Multi Drugs Therapy standardized by WHO. Previous study in China found that HLA-B 13:01 is strongly associated to DHS in China population. Aim of this study was to detect HLA- B 13:01 gene among DHS leprosy patients in Papua using Sequence Based Typing Methods and qPCR method. This was cross sectional study among 34 leprosy patients that presented with DHS from Papua and West Papua Province. As the negative control we screened 52 leprosy patients that confirmed non-DHS patients. DNA was extracted from 3 mL blood specimens. HLA-B alleles were detected using Sequence Based Typing method by TBG kit and qPCR rapid detection kit using Nalagenetic. The results of HLA-sequence based typing among the 34 leprosy with DHS patients showed that 91,17 % were positively HLA-B:13:01 HLA-B 13:01 carrier consist of 82,35% Heterozygous and 8.82% Homozygous. In negative control 96,15% were non carrier of HLA-B 13:01 gene and 3,85% were heterozygous. Using qPCR procedure, HLA-B 13:01 was positively detected in DHS-leprosy patients and 96.15% negatively detected in Non-DHS leprosy patients but positive in 3.85% of heterozygote non-DHS. The concordances between SBT and qPCR methods were 100%. Conclusion of this study was HLA-B 13:01 gene can be detected in most of DHS leprosy patients in Papua Ethnics Group using SBT as well as qPCR rapid detection kit.