Quantum-enhanced stimulated emission detection for label-free microscopy
Nonlinear optical microscopy techniques have emerged as a set of successful tools for biological imaging. Stimulated emission microscopy belongs to a small subset of pump–probe techniques, which can image non-fluorescent samples without requiring fluorescent labeling. However, its sensitivity has be...
Gespeichert in:
| Veröffentlicht in: | Applied physics letters 2020-07, Vol.117 (2) |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 2 |
| container_start_page | |
| container_title | Applied physics letters |
| container_volume | 117 |
| creator | Triginer Garces, Gil Chrzanowski, Helen M. Daryanoosh, Shakib Thiel, Valerian Marchant, Anna L. Patel, Raj B. Humphreys, Peter C. Datta, Animesh Walmsley, Ian A. |
| description | Nonlinear optical microscopy techniques have emerged as a set of successful tools for biological imaging. Stimulated emission microscopy belongs to a small subset of pump–probe techniques, which can image non-fluorescent samples without requiring fluorescent labeling. However, its sensitivity has been shown to be ultimately limited by the quantum fluctuations in the probe beam. We propose and experimentally implement sub-shot-noise limited stimulated emission microscopy by preparing the probe pulse in an intensity-squeezed state. This technique paves the way for imaging delicate biological samples that have no detectable fluorescence with sensitivity beyond standard quantum fluctuations. |
| doi_str_mv | 10.1063/5.0009681 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_scita</sourceid><recordid>TN_cdi_scitation_primary_10_1063_5_0009681</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>2423325740</sourcerecordid><originalsourceid>FETCH-LOGICAL-c393t-546eec4cda9dcacb81904078721ea7d5df27934952a309a0e9e1df06f330b5e73</originalsourceid><addsrcrecordid>eNqdkE9LxDAQxYMouP45-A0KnhSyJk3TNEdZ1BUWRNBzyCYT7NI2NUmF_fam7IJ3T_Nm-DEz7yF0Q8mSkpo98CUhRNYNPUELSoTAjNLmFC3ylOFacnqOLmLc5ZaXjC3Q-n3SQ5p6DMOXHgzYIqa2nzqdsoS-jbH1Q2EhgUmzcj4Und5Ch10AKPrWBB-NH_dX6MzpLsL1sV6iz-enj9Uab95eXlePG2yYZAnzqgYwlbFaWqPNtqGSVEQ0oqSgheXWlUKySvJSMyI1AQnUOlI7xsiWg2CX6Pawdwz-e4KY1M5PYcgnVVllSyUXFcnU3YGa34sBnBpD2-uwV5SoOSjF1TGozN4f2GjapGeX_4N_fPgD1Wgd-wXuV3bP</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2423325740</pqid></control><display><type>article</type><title>Quantum-enhanced stimulated emission detection for label-free microscopy</title><source>AIP Journals Complete</source><source>Alma/SFX Local Collection</source><creator>Triginer Garces, Gil ; Chrzanowski, Helen M. ; Daryanoosh, Shakib ; Thiel, Valerian ; Marchant, Anna L. ; Patel, Raj B. ; Humphreys, Peter C. ; Datta, Animesh ; Walmsley, Ian A.</creator><creatorcontrib>Triginer Garces, Gil ; Chrzanowski, Helen M. ; Daryanoosh, Shakib ; Thiel, Valerian ; Marchant, Anna L. ; Patel, Raj B. ; Humphreys, Peter C. ; Datta, Animesh ; Walmsley, Ian A.</creatorcontrib><description>Nonlinear optical microscopy techniques have emerged as a set of successful tools for biological imaging. Stimulated emission microscopy belongs to a small subset of pump–probe techniques, which can image non-fluorescent samples without requiring fluorescent labeling. However, its sensitivity has been shown to be ultimately limited by the quantum fluctuations in the probe beam. We propose and experimentally implement sub-shot-noise limited stimulated emission microscopy by preparing the probe pulse in an intensity-squeezed state. This technique paves the way for imaging delicate biological samples that have no detectable fluorescence with sensitivity beyond standard quantum fluctuations.</description><identifier>ISSN: 0003-6951</identifier><identifier>EISSN: 1077-3118</identifier><identifier>DOI: 10.1063/5.0009681</identifier><identifier>CODEN: APPLAB</identifier><language>eng</language><publisher>Melville: American Institute of Physics</publisher><subject>Applied physics ; Biological properties ; Emission microscopy ; Emissions control ; Fluorescence ; Microscopy ; Optical microscopy ; Sensitivity ; Squeezed states (quantum theory) ; Stimulated emission</subject><ispartof>Applied physics letters, 2020-07, Vol.117 (2)</ispartof><rights>Author(s)</rights><rights>2020 Author(s). Published under license by AIP Publishing.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c393t-546eec4cda9dcacb81904078721ea7d5df27934952a309a0e9e1df06f330b5e73</citedby><cites>FETCH-LOGICAL-c393t-546eec4cda9dcacb81904078721ea7d5df27934952a309a0e9e1df06f330b5e73</cites><orcidid>0000-0002-7382-978X ; 0000-0003-4021-4655 ; 0000-0001-8627-1298 ; 0000-0002-6350-4842</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://pubs.aip.org/apl/article-lookup/doi/10.1063/5.0009681$$EHTML$$P50$$Gscitation$$H</linktohtml><link.rule.ids>314,776,780,790,4498,27901,27902,76353</link.rule.ids></links><search><creatorcontrib>Triginer Garces, Gil</creatorcontrib><creatorcontrib>Chrzanowski, Helen M.</creatorcontrib><creatorcontrib>Daryanoosh, Shakib</creatorcontrib><creatorcontrib>Thiel, Valerian</creatorcontrib><creatorcontrib>Marchant, Anna L.</creatorcontrib><creatorcontrib>Patel, Raj B.</creatorcontrib><creatorcontrib>Humphreys, Peter C.</creatorcontrib><creatorcontrib>Datta, Animesh</creatorcontrib><creatorcontrib>Walmsley, Ian A.</creatorcontrib><title>Quantum-enhanced stimulated emission detection for label-free microscopy</title><title>Applied physics letters</title><description>Nonlinear optical microscopy techniques have emerged as a set of successful tools for biological imaging. Stimulated emission microscopy belongs to a small subset of pump–probe techniques, which can image non-fluorescent samples without requiring fluorescent labeling. However, its sensitivity has been shown to be ultimately limited by the quantum fluctuations in the probe beam. We propose and experimentally implement sub-shot-noise limited stimulated emission microscopy by preparing the probe pulse in an intensity-squeezed state. This technique paves the way for imaging delicate biological samples that have no detectable fluorescence with sensitivity beyond standard quantum fluctuations.</description><subject>Applied physics</subject><subject>Biological properties</subject><subject>Emission microscopy</subject><subject>Emissions control</subject><subject>Fluorescence</subject><subject>Microscopy</subject><subject>Optical microscopy</subject><subject>Sensitivity</subject><subject>Squeezed states (quantum theory)</subject><subject>Stimulated emission</subject><issn>0003-6951</issn><issn>1077-3118</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><recordid>eNqdkE9LxDAQxYMouP45-A0KnhSyJk3TNEdZ1BUWRNBzyCYT7NI2NUmF_fam7IJ3T_Nm-DEz7yF0Q8mSkpo98CUhRNYNPUELSoTAjNLmFC3ylOFacnqOLmLc5ZaXjC3Q-n3SQ5p6DMOXHgzYIqa2nzqdsoS-jbH1Q2EhgUmzcj4Und5Ch10AKPrWBB-NH_dX6MzpLsL1sV6iz-enj9Uab95eXlePG2yYZAnzqgYwlbFaWqPNtqGSVEQ0oqSgheXWlUKySvJSMyI1AQnUOlI7xsiWg2CX6Pawdwz-e4KY1M5PYcgnVVllSyUXFcnU3YGa34sBnBpD2-uwV5SoOSjF1TGozN4f2GjapGeX_4N_fPgD1Wgd-wXuV3bP</recordid><startdate>20200713</startdate><enddate>20200713</enddate><creator>Triginer Garces, Gil</creator><creator>Chrzanowski, Helen M.</creator><creator>Daryanoosh, Shakib</creator><creator>Thiel, Valerian</creator><creator>Marchant, Anna L.</creator><creator>Patel, Raj B.</creator><creator>Humphreys, Peter C.</creator><creator>Datta, Animesh</creator><creator>Walmsley, Ian A.</creator><general>American Institute of Physics</general><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>H8D</scope><scope>L7M</scope><orcidid>https://orcid.org/0000-0002-7382-978X</orcidid><orcidid>https://orcid.org/0000-0003-4021-4655</orcidid><orcidid>https://orcid.org/0000-0001-8627-1298</orcidid><orcidid>https://orcid.org/0000-0002-6350-4842</orcidid></search><sort><creationdate>20200713</creationdate><title>Quantum-enhanced stimulated emission detection for label-free microscopy</title><author>Triginer Garces, Gil ; Chrzanowski, Helen M. ; Daryanoosh, Shakib ; Thiel, Valerian ; Marchant, Anna L. ; Patel, Raj B. ; Humphreys, Peter C. ; Datta, Animesh ; Walmsley, Ian A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c393t-546eec4cda9dcacb81904078721ea7d5df27934952a309a0e9e1df06f330b5e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Applied physics</topic><topic>Biological properties</topic><topic>Emission microscopy</topic><topic>Emissions control</topic><topic>Fluorescence</topic><topic>Microscopy</topic><topic>Optical microscopy</topic><topic>Sensitivity</topic><topic>Squeezed states (quantum theory)</topic><topic>Stimulated emission</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Triginer Garces, Gil</creatorcontrib><creatorcontrib>Chrzanowski, Helen M.</creatorcontrib><creatorcontrib>Daryanoosh, Shakib</creatorcontrib><creatorcontrib>Thiel, Valerian</creatorcontrib><creatorcontrib>Marchant, Anna L.</creatorcontrib><creatorcontrib>Patel, Raj B.</creatorcontrib><creatorcontrib>Humphreys, Peter C.</creatorcontrib><creatorcontrib>Datta, Animesh</creatorcontrib><creatorcontrib>Walmsley, Ian A.</creatorcontrib><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Aerospace Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Applied physics letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Triginer Garces, Gil</au><au>Chrzanowski, Helen M.</au><au>Daryanoosh, Shakib</au><au>Thiel, Valerian</au><au>Marchant, Anna L.</au><au>Patel, Raj B.</au><au>Humphreys, Peter C.</au><au>Datta, Animesh</au><au>Walmsley, Ian A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantum-enhanced stimulated emission detection for label-free microscopy</atitle><jtitle>Applied physics letters</jtitle><date>2020-07-13</date><risdate>2020</risdate><volume>117</volume><issue>2</issue><issn>0003-6951</issn><eissn>1077-3118</eissn><coden>APPLAB</coden><abstract>Nonlinear optical microscopy techniques have emerged as a set of successful tools for biological imaging. Stimulated emission microscopy belongs to a small subset of pump–probe techniques, which can image non-fluorescent samples without requiring fluorescent labeling. However, its sensitivity has been shown to be ultimately limited by the quantum fluctuations in the probe beam. We propose and experimentally implement sub-shot-noise limited stimulated emission microscopy by preparing the probe pulse in an intensity-squeezed state. This technique paves the way for imaging delicate biological samples that have no detectable fluorescence with sensitivity beyond standard quantum fluctuations.</abstract><cop>Melville</cop><pub>American Institute of Physics</pub><doi>10.1063/5.0009681</doi><tpages>5</tpages><orcidid>https://orcid.org/0000-0002-7382-978X</orcidid><orcidid>https://orcid.org/0000-0003-4021-4655</orcidid><orcidid>https://orcid.org/0000-0001-8627-1298</orcidid><orcidid>https://orcid.org/0000-0002-6350-4842</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-6951 |
| ispartof | Applied physics letters, 2020-07, Vol.117 (2) |
| issn | 0003-6951 1077-3118 |
| language | eng |
| recordid | cdi_scitation_primary_10_1063_5_0009681 |
| source | AIP Journals Complete; Alma/SFX Local Collection |
| subjects | Applied physics Biological properties Emission microscopy Emissions control Fluorescence Microscopy Optical microscopy Sensitivity Squeezed states (quantum theory) Stimulated emission |
| title | Quantum-enhanced stimulated emission detection for label-free microscopy |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T04%3A00%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_scita&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Quantum-enhanced%20stimulated%20emission%20detection%20for%20label-free%20microscopy&rft.jtitle=Applied%20physics%20letters&rft.au=Triginer%20Garces,%20Gil&rft.date=2020-07-13&rft.volume=117&rft.issue=2&rft.issn=0003-6951&rft.eissn=1077-3118&rft.coden=APPLAB&rft_id=info:doi/10.1063/5.0009681&rft_dat=%3Cproquest_scita%3E2423325740%3C/proquest_scita%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2423325740&rft_id=info:pmid/&rfr_iscdi=true |