The growth of microalgae Chlorococcum sp. isolated from Ampenan estuary of Lombok Island in Walne’s medium
Microalgae, also known as plankton, are one of the aquatic organisms which conduct photosynthesis similar to higher plants. Microalgae can live in any aquatic environment and are relatively easy to culture. There have been many studies that have shown the commercial applications of microalgae which...
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creator | Putri, Dina Soes Astuti, Sri Puji Alaa, Siti |
description | Microalgae, also known as plankton, are one of the aquatic organisms which conduct photosynthesis similar to higher plants. Microalgae can live in any aquatic environment and are relatively easy to culture. There have been many studies that have shown the commercial applications of microalgae which have been continuously driving the development of microalgae-related research for decades. As for this research, the aim was to determine the growth phase of microalgae Chlorococcum sp isolated from Ampenan Beach estuary of Lombok Island. In this study, isolate from solid medium obtained from previous research were transferred to liquid medium enriched with Walne’s nutrition. The conditions of the culture were: room temperature (25 °C), light intensity of 2000-3000 lux, photo period of light:dark (24:0) hours, pH range 7-8, 24 hours of aeration, and culture under sterile environment. Based on every day observation of cell numbers, it is known that Chlorococcum sp began to enter the death phase on the fifth day so that the observations were only carried out for seven consecutive days. The initial cell number was 187,500 cells/ml and the optimum cell numbers (on the fourth day) was 331,250 cells/ml. In conclusion, the optimum growth of microalgae occurred on the fourth day from the first day of culture and it began to enter its death phase on the fifth day. |
doi_str_mv | 10.1063/1.5141301 |
format | Conference Proceeding |
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Microalgae can live in any aquatic environment and are relatively easy to culture. There have been many studies that have shown the commercial applications of microalgae which have been continuously driving the development of microalgae-related research for decades. As for this research, the aim was to determine the growth phase of microalgae Chlorococcum sp isolated from Ampenan Beach estuary of Lombok Island. In this study, isolate from solid medium obtained from previous research were transferred to liquid medium enriched with Walne’s nutrition. The conditions of the culture were: room temperature (25 °C), light intensity of 2000-3000 lux, photo period of light:dark (24:0) hours, pH range 7-8, 24 hours of aeration, and culture under sterile environment. Based on every day observation of cell numbers, it is known that Chlorococcum sp began to enter the death phase on the fifth day so that the observations were only carried out for seven consecutive days. The initial cell number was 187,500 cells/ml and the optimum cell numbers (on the fourth day) was 331,250 cells/ml. In conclusion, the optimum growth of microalgae occurred on the fourth day from the first day of culture and it began to enter its death phase on the fifth day.</description><identifier>ISSN: 0094-243X</identifier><identifier>EISSN: 1551-7616</identifier><identifier>DOI: 10.1063/1.5141301</identifier><identifier>CODEN: APCPCS</identifier><language>eng</language><publisher>Melville: American Institute of Physics</publisher><subject>Aeration ; Algae ; Aquatic environment ; Estuaries ; Luminous intensity ; Nutrition ; Photosynthesis ; Plankton ; Room temperature</subject><ispartof>AIP conference proceedings, 2019, Vol.2199 (1)</ispartof><rights>Author(s)</rights><rights>2019 Author(s). 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The initial cell number was 187,500 cells/ml and the optimum cell numbers (on the fourth day) was 331,250 cells/ml. In conclusion, the optimum growth of microalgae occurred on the fourth day from the first day of culture and it began to enter its death phase on the fifth day.</description><subject>Aeration</subject><subject>Algae</subject><subject>Aquatic environment</subject><subject>Estuaries</subject><subject>Luminous intensity</subject><subject>Nutrition</subject><subject>Photosynthesis</subject><subject>Plankton</subject><subject>Room temperature</subject><issn>0094-243X</issn><issn>1551-7616</issn><fulltext>true</fulltext><rsrctype>conference_proceeding</rsrctype><creationdate>2019</creationdate><recordtype>conference_proceeding</recordtype><recordid>eNp9kE1OwzAUhC0EEqWw4AaW2CGl-PknTpZVxU-lSmyKYBe5jt2mxHGwExA7rsH1OAmpWokdq9l8M-_NIHQJZAIkZTcwEcCBEThCIxACEplCeoxGhOQ8oZy9nKKzGLeE0FzKbITq5cbgdfAf3QZ7i12lg1f1Whk829Q-eO217h2O7QRX0deqMyW2wTs8da1pVINN7HoVPnfmhXcr_4rnsVZNiasGP6u6MT9f3xE7U1a9O0cnVtXRXBx0jJ7ubpezh2TxeD-fTReJZjTrklxLJTUoJbi0YKTUjFhurVhRJoQiwFJLBQhZCktJJnlaGjYYUsuBG0jZGF3tc9vg3_rhw2Lr-9AMJwvKaJ5nnOcwUNd7KuqqU13lm6INlRvKFECK3ZoFFIc1_4PfffgDi7a07Bdog3Yc</recordid><startdate>20191223</startdate><enddate>20191223</enddate><creator>Putri, Dina Soes</creator><creator>Astuti, Sri Puji</creator><creator>Alaa, Siti</creator><general>American Institute of Physics</general><scope>8FD</scope><scope>H8D</scope><scope>L7M</scope></search><sort><creationdate>20191223</creationdate><title>The growth of microalgae Chlorococcum sp. isolated from Ampenan estuary of Lombok Island in Walne’s medium</title><author>Putri, Dina Soes ; Astuti, Sri Puji ; Alaa, Siti</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c328t-9c7a7c1aa547f1e77c30f4ff5b2355a0136f25157d5f208746de37c16f414e163</frbrgroupid><rsrctype>conference_proceedings</rsrctype><prefilter>conference_proceedings</prefilter><language>eng</language><creationdate>2019</creationdate><topic>Aeration</topic><topic>Algae</topic><topic>Aquatic environment</topic><topic>Estuaries</topic><topic>Luminous intensity</topic><topic>Nutrition</topic><topic>Photosynthesis</topic><topic>Plankton</topic><topic>Room temperature</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Putri, Dina Soes</creatorcontrib><creatorcontrib>Astuti, Sri Puji</creatorcontrib><creatorcontrib>Alaa, Siti</creatorcontrib><collection>Technology Research Database</collection><collection>Aerospace Database</collection><collection>Advanced Technologies Database with Aerospace</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Putri, Dina Soes</au><au>Astuti, Sri Puji</au><au>Alaa, Siti</au><au>Martyasari, Ni Wayan Riyani</au><au>Prasedya, Eka Sunarwidhi</au><format>book</format><genre>proceeding</genre><ristype>CONF</ristype><atitle>The growth of microalgae Chlorococcum sp. isolated from Ampenan estuary of Lombok Island in Walne’s medium</atitle><btitle>AIP conference proceedings</btitle><date>2019-12-23</date><risdate>2019</risdate><volume>2199</volume><issue>1</issue><issn>0094-243X</issn><eissn>1551-7616</eissn><coden>APCPCS</coden><abstract>Microalgae, also known as plankton, are one of the aquatic organisms which conduct photosynthesis similar to higher plants. Microalgae can live in any aquatic environment and are relatively easy to culture. There have been many studies that have shown the commercial applications of microalgae which have been continuously driving the development of microalgae-related research for decades. As for this research, the aim was to determine the growth phase of microalgae Chlorococcum sp isolated from Ampenan Beach estuary of Lombok Island. In this study, isolate from solid medium obtained from previous research were transferred to liquid medium enriched with Walne’s nutrition. The conditions of the culture were: room temperature (25 °C), light intensity of 2000-3000 lux, photo period of light:dark (24:0) hours, pH range 7-8, 24 hours of aeration, and culture under sterile environment. Based on every day observation of cell numbers, it is known that Chlorococcum sp began to enter the death phase on the fifth day so that the observations were only carried out for seven consecutive days. The initial cell number was 187,500 cells/ml and the optimum cell numbers (on the fourth day) was 331,250 cells/ml. In conclusion, the optimum growth of microalgae occurred on the fourth day from the first day of culture and it began to enter its death phase on the fifth day.</abstract><cop>Melville</cop><pub>American Institute of Physics</pub><doi>10.1063/1.5141301</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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identifier | ISSN: 0094-243X |
ispartof | AIP conference proceedings, 2019, Vol.2199 (1) |
issn | 0094-243X 1551-7616 |
language | eng |
recordid | cdi_scitation_primary_10_1063_1_5141301 |
source | AIP Journals Complete |
subjects | Aeration Algae Aquatic environment Estuaries Luminous intensity Nutrition Photosynthesis Plankton Room temperature |
title | The growth of microalgae Chlorococcum sp. isolated from Ampenan estuary of Lombok Island in Walne’s medium |
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