Differential effect of heated and non-heated gelatin samples on the quality and quantity of extracted DNA
DNA concentration and purification are the major criteria for analysis of gelatin sources using molecular technique such as DNA-based method. In this study, DNA from heated and non-heated of different types of gelatin samples were extracted using commercial kit. The measurements of concentration and...
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| creator | Rahman, Muhammad Husaini Abdul Hassan, Mohd Sukri |
| description | DNA concentration and purification are the major criteria for analysis of gelatin sources using molecular technique such as DNA-based method. In this study, DNA from heated and non-heated of different types of gelatin samples were extracted using commercial kit. The measurements of concentration and purity from heated and non-heated samples were compared. The result has highlighted that heated samples yield higher DNA concentration compared to non-heated samples. In terms of DNA purity, non-heated samples were found to be lower than the expected A260/A280 interval values of 1.8-2.2. Besides, coefficient variation (CV) also has been used to compare variability between different steps. |
| doi_str_mv | 10.1063/1.5041237 |
| format | Conference Proceeding |
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In this study, DNA from heated and non-heated of different types of gelatin samples were extracted using commercial kit. The measurements of concentration and purity from heated and non-heated samples were compared. The result has highlighted that heated samples yield higher DNA concentration compared to non-heated samples. In terms of DNA purity, non-heated samples were found to be lower than the expected A260/A280 interval values of 1.8-2.2. Besides, coefficient variation (CV) also has been used to compare variability between different steps.</description><identifier>ISSN: 0094-243X</identifier><identifier>EISSN: 1551-7616</identifier><identifier>DOI: 10.1063/1.5041237</identifier><identifier>CODEN: APCPCS</identifier><language>eng</language><publisher>Melville: American Institute of Physics</publisher><subject>Coefficient of variation ; Deoxyribonucleic acid ; DNA ; Gelatin ; Heat treating ; Purity</subject><ispartof>AIP Conference Proceedings, 2018, Vol.1972 (1)</ispartof><rights>Author(s)</rights><rights>2018 Author(s). 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In this study, DNA from heated and non-heated of different types of gelatin samples were extracted using commercial kit. The measurements of concentration and purity from heated and non-heated samples were compared. The result has highlighted that heated samples yield higher DNA concentration compared to non-heated samples. In terms of DNA purity, non-heated samples were found to be lower than the expected A260/A280 interval values of 1.8-2.2. Besides, coefficient variation (CV) also has been used to compare variability between different steps.</description><subject>Coefficient of variation</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>Gelatin</subject><subject>Heat treating</subject><subject>Purity</subject><issn>0094-243X</issn><issn>1551-7616</issn><fulltext>true</fulltext><rsrctype>conference_proceeding</rsrctype><creationdate>2018</creationdate><recordtype>conference_proceeding</recordtype><recordid>eNp9kEtLAzEUhYMoWKsL_0HAnTA1N49JuiytLyi6UXAXMjOJnTLNTCep2H9vagfcubrnwHfO5V6EroFMgOTsDiaCcKBMnqARCAGZzCE_RSNCpjyjnH2co4sQ1oTQqZRqhOpF7ZztrY-1abBNuoy4dXhlTbQVNr7CvvXZYD9tY2LtcTCbrrEBtx7HlcXbnWnquP-lk05dyaQS-x17Ux6Ci5fZJTpzpgn2aphj9P5w_zZ_ypavj8_z2TLrqFIxM0xyqEBNVQUGOAWeE5szSklhiKRWFOC4cRJYQUsuTO6oLCouS0Wo4IVlY3Rz7O36druzIep1u-t9WqkpUYoJxghN1O2RCmUd002t111fb0y_119tr0EPb9Rd5f6DgejD3_8C7AcGc3P8</recordid><startdate>20180605</startdate><enddate>20180605</enddate><creator>Rahman, Muhammad Husaini Abdul</creator><creator>Hassan, Mohd Sukri</creator><general>American Institute of Physics</general><scope>8FD</scope><scope>H8D</scope><scope>L7M</scope></search><sort><creationdate>20180605</creationdate><title>Differential effect of heated and non-heated gelatin samples on the quality and quantity of extracted DNA</title><author>Rahman, Muhammad Husaini Abdul ; Hassan, Mohd Sukri</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-p288t-a3741d1898d1a1421460e63220ba072e5b1f4af713b2c45a6f27bd47c80254be3</frbrgroupid><rsrctype>conference_proceedings</rsrctype><prefilter>conference_proceedings</prefilter><language>eng</language><creationdate>2018</creationdate><topic>Coefficient of variation</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>Gelatin</topic><topic>Heat treating</topic><topic>Purity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rahman, Muhammad Husaini Abdul</creatorcontrib><creatorcontrib>Hassan, Mohd Sukri</creatorcontrib><collection>Technology Research Database</collection><collection>Aerospace Database</collection><collection>Advanced Technologies Database with Aerospace</collection></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rahman, Muhammad Husaini Abdul</au><au>Hassan, Mohd Sukri</au><au>Harun, Farah Wahida</au><au>Yatim, Nadhrah Md</au><au>Ali, Ernie Suzana</au><format>book</format><genre>proceeding</genre><ristype>CONF</ristype><atitle>Differential effect of heated and non-heated gelatin samples on the quality and quantity of extracted DNA</atitle><btitle>AIP Conference Proceedings</btitle><date>2018-06-05</date><risdate>2018</risdate><volume>1972</volume><issue>1</issue><issn>0094-243X</issn><eissn>1551-7616</eissn><coden>APCPCS</coden><abstract>DNA concentration and purification are the major criteria for analysis of gelatin sources using molecular technique such as DNA-based method. In this study, DNA from heated and non-heated of different types of gelatin samples were extracted using commercial kit. The measurements of concentration and purity from heated and non-heated samples were compared. The result has highlighted that heated samples yield higher DNA concentration compared to non-heated samples. In terms of DNA purity, non-heated samples were found to be lower than the expected A260/A280 interval values of 1.8-2.2. Besides, coefficient variation (CV) also has been used to compare variability between different steps.</abstract><cop>Melville</cop><pub>American Institute of Physics</pub><doi>10.1063/1.5041237</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0094-243X |
| ispartof | AIP Conference Proceedings, 2018, Vol.1972 (1) |
| issn | 0094-243X 1551-7616 |
| language | eng |
| recordid | cdi_scitation_primary_10_1063_1_5041237 |
| source | AIP Journals Complete |
| subjects | Coefficient of variation Deoxyribonucleic acid DNA Gelatin Heat treating Purity |
| title | Differential effect of heated and non-heated gelatin samples on the quality and quantity of extracted DNA |
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