Two-photon excitation microfluorometer for multiplexed single-step bioaffinity assays

Two-photon excitation microfluorometer for multiplexed single-step bioaffinity assays

A new type of instrumentation for single-step bioaffinity assays and microvolume fluorometry is presented. The concept is based on the use of two-photon excitation by a low-cost near-infrared laser and individual observation of bioactive fluorescent microparticles. The applicability of the instrumen...

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Veröffentlicht in:Review of scientific instruments 2002-07, Vol.73 (7), p.2680-2685
Hauptverfasser: Soini, Juhani T., Soukka, Jori M., Soini, Erkki, Hänninen, Pekka E.
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container_issue 7
container_start_page 2680
container_title Review of scientific instruments
container_volume 73
creator Soini, Juhani T.
Soukka, Jori M.
Soini, Erkki
Hänninen, Pekka E.
description A new type of instrumentation for single-step bioaffinity assays and microvolume fluorometry is presented. The concept is based on the use of two-photon excitation by a low-cost near-infrared laser and individual observation of bioactive fluorescent microparticles. The applicability of the instrument is demonstrated by a microparticle based multiplexed bioaffinity assay where several fluorescent markers are simultaneously excited. This instrument can be applied in the growing fields of drug discovery, in life science research, and in routine laboratory diagnostics.
doi_str_mv 10.1063/1.1485782
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title Two-photon excitation microfluorometer for multiplexed single-step bioaffinity assays
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