Development of low-temperature and high vacuum atomic force microscope with freeze–fracture function
A high vacuum low temperature atomic force microscope (AFM) for the direct observation of biological freeze–fracture samples has been developed. This AFM has an integrated vacuum system and a freeze–fracture mechanism inside the vacuum chamber. It is possible to observe the fractured sample surface...
Gespeichert in:
| Veröffentlicht in: | Review of scientific instruments 2001-02, Vol.72 (2), p.1445-1448 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1448 |
|---|---|
| container_issue | 2 |
| container_start_page | 1445 |
| container_title | Review of scientific instruments |
| container_volume | 72 |
| creator | Nakamoto, Keiichi Mooney, C. B. Iwatsuki, Masashi |
| description | A high vacuum low temperature atomic force microscope (AFM) for the direct observation of biological freeze–fracture samples has been developed. This AFM has an integrated vacuum system and a freeze–fracture mechanism inside the vacuum chamber. It is possible to observe the fractured sample surface without exposing the freshly fractured surface to the ambient atmosphere. The design is described in detail. A sample temperature up to −175 °C is achieved, with the temperature fluctuation less than 0.1 °C. Temperature control is possible from −175 to 0 °C for deep etching. An ultimate vacuum pressure of
8.7×10
−6
Pa has been achieved. Freeze-fractured human red blood cell has been examined, and fine structure within the cell has been observed. It is demonstrated that this is convenient and useful for the direct observation of a biological freeze–fractured surface. |
| doi_str_mv | 10.1063/1.1342033 |
| format | Article |
| fullrecord | <record><control><sourceid>scitation_cross</sourceid><recordid>TN_cdi_scitation_primary_10_1063_1_1342033</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>rsi</sourcerecordid><originalsourceid>FETCH-LOGICAL-c334t-cad9318d7443875fbba99f47a8c2dfa1b21d609a701a95ddecd89956a962c03e3</originalsourceid><addsrcrecordid>eNp9kM1OwzAQhC0EEqVw4A18BSnFjp0fH1H5lSpxgXO0sdc0qIkjx2kFJ96BN-RJSGgFByTmsnP4djQaQk45m3GWigs-40LGTIg9MuEsV1GWxmKfTBgTMkozmR-So657YYMSzifEXuEaV66tsQnUWbpymyhg3aKH0Huk0Bi6rJ6XdA2672sKwdWVptZ5jXRw3nXatUg3VVhS6xHf8PP9w3rQ3--2b3SoXHNMDiysOjzZ3Sl5url-nN9Fi4fb-_nlItJCyBBpMErw3GRSijxLbFmCUlZmkOvYWOBlzE3KFGSMg0qMQW1ypZIUVBprJlBMydk2dyzWebRF66sa_GvBWTEOVPBiN9DAnm_ZTlcBxpY_8Nr5X7Bojf0P_pv8BQo3d4g</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Development of low-temperature and high vacuum atomic force microscope with freeze–fracture function</title><source>AIP Journals Complete</source><source>AIP Digital Archive</source><creator>Nakamoto, Keiichi ; Mooney, C. B. ; Iwatsuki, Masashi</creator><creatorcontrib>Nakamoto, Keiichi ; Mooney, C. B. ; Iwatsuki, Masashi</creatorcontrib><description>A high vacuum low temperature atomic force microscope (AFM) for the direct observation of biological freeze–fracture samples has been developed. This AFM has an integrated vacuum system and a freeze–fracture mechanism inside the vacuum chamber. It is possible to observe the fractured sample surface without exposing the freshly fractured surface to the ambient atmosphere. The design is described in detail. A sample temperature up to −175 °C is achieved, with the temperature fluctuation less than 0.1 °C. Temperature control is possible from −175 to 0 °C for deep etching. An ultimate vacuum pressure of
8.7×10
−6
Pa has been achieved. Freeze-fractured human red blood cell has been examined, and fine structure within the cell has been observed. It is demonstrated that this is convenient and useful for the direct observation of a biological freeze–fractured surface.</description><identifier>ISSN: 0034-6748</identifier><identifier>EISSN: 1089-7623</identifier><identifier>DOI: 10.1063/1.1342033</identifier><identifier>CODEN: RSINAK</identifier><language>eng</language><ispartof>Review of scientific instruments, 2001-02, Vol.72 (2), p.1445-1448</ispartof><rights>American Institute of Physics</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c334t-cad9318d7443875fbba99f47a8c2dfa1b21d609a701a95ddecd89956a962c03e3</citedby><cites>FETCH-LOGICAL-c334t-cad9318d7443875fbba99f47a8c2dfa1b21d609a701a95ddecd89956a962c03e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://pubs.aip.org/rsi/article-lookup/doi/10.1063/1.1342033$$EHTML$$P50$$Gscitation$$H</linktohtml><link.rule.ids>314,776,780,790,1553,4498,27901,27902,76127,76133</link.rule.ids></links><search><creatorcontrib>Nakamoto, Keiichi</creatorcontrib><creatorcontrib>Mooney, C. B.</creatorcontrib><creatorcontrib>Iwatsuki, Masashi</creatorcontrib><title>Development of low-temperature and high vacuum atomic force microscope with freeze–fracture function</title><title>Review of scientific instruments</title><description>A high vacuum low temperature atomic force microscope (AFM) for the direct observation of biological freeze–fracture samples has been developed. This AFM has an integrated vacuum system and a freeze–fracture mechanism inside the vacuum chamber. It is possible to observe the fractured sample surface without exposing the freshly fractured surface to the ambient atmosphere. The design is described in detail. A sample temperature up to −175 °C is achieved, with the temperature fluctuation less than 0.1 °C. Temperature control is possible from −175 to 0 °C for deep etching. An ultimate vacuum pressure of
8.7×10
−6
Pa has been achieved. Freeze-fractured human red blood cell has been examined, and fine structure within the cell has been observed. It is demonstrated that this is convenient and useful for the direct observation of a biological freeze–fractured surface.</description><issn>0034-6748</issn><issn>1089-7623</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2001</creationdate><recordtype>article</recordtype><recordid>eNp9kM1OwzAQhC0EEqVw4A18BSnFjp0fH1H5lSpxgXO0sdc0qIkjx2kFJ96BN-RJSGgFByTmsnP4djQaQk45m3GWigs-40LGTIg9MuEsV1GWxmKfTBgTMkozmR-So657YYMSzifEXuEaV66tsQnUWbpymyhg3aKH0Huk0Bi6rJ6XdA2672sKwdWVptZ5jXRw3nXatUg3VVhS6xHf8PP9w3rQ3--2b3SoXHNMDiysOjzZ3Sl5url-nN9Fi4fb-_nlItJCyBBpMErw3GRSijxLbFmCUlZmkOvYWOBlzE3KFGSMg0qMQW1ypZIUVBprJlBMydk2dyzWebRF66sa_GvBWTEOVPBiN9DAnm_ZTlcBxpY_8Nr5X7Bojf0P_pv8BQo3d4g</recordid><startdate>20010201</startdate><enddate>20010201</enddate><creator>Nakamoto, Keiichi</creator><creator>Mooney, C. B.</creator><creator>Iwatsuki, Masashi</creator><scope>AAYXX</scope><scope>CITATION</scope></search><sort><creationdate>20010201</creationdate><title>Development of low-temperature and high vacuum atomic force microscope with freeze–fracture function</title><author>Nakamoto, Keiichi ; Mooney, C. B. ; Iwatsuki, Masashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c334t-cad9318d7443875fbba99f47a8c2dfa1b21d609a701a95ddecd89956a962c03e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2001</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nakamoto, Keiichi</creatorcontrib><creatorcontrib>Mooney, C. B.</creatorcontrib><creatorcontrib>Iwatsuki, Masashi</creatorcontrib><collection>CrossRef</collection><jtitle>Review of scientific instruments</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Nakamoto, Keiichi</au><au>Mooney, C. B.</au><au>Iwatsuki, Masashi</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Development of low-temperature and high vacuum atomic force microscope with freeze–fracture function</atitle><jtitle>Review of scientific instruments</jtitle><date>2001-02-01</date><risdate>2001</risdate><volume>72</volume><issue>2</issue><spage>1445</spage><epage>1448</epage><pages>1445-1448</pages><issn>0034-6748</issn><eissn>1089-7623</eissn><coden>RSINAK</coden><abstract>A high vacuum low temperature atomic force microscope (AFM) for the direct observation of biological freeze–fracture samples has been developed. This AFM has an integrated vacuum system and a freeze–fracture mechanism inside the vacuum chamber. It is possible to observe the fractured sample surface without exposing the freshly fractured surface to the ambient atmosphere. The design is described in detail. A sample temperature up to −175 °C is achieved, with the temperature fluctuation less than 0.1 °C. Temperature control is possible from −175 to 0 °C for deep etching. An ultimate vacuum pressure of
8.7×10
−6
Pa has been achieved. Freeze-fractured human red blood cell has been examined, and fine structure within the cell has been observed. It is demonstrated that this is convenient and useful for the direct observation of a biological freeze–fractured surface.</abstract><doi>10.1063/1.1342033</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0034-6748 |
| ispartof | Review of scientific instruments, 2001-02, Vol.72 (2), p.1445-1448 |
| issn | 0034-6748 1089-7623 |
| language | eng |
| recordid | cdi_scitation_primary_10_1063_1_1342033 |
| source | AIP Journals Complete; AIP Digital Archive |
| title | Development of low-temperature and high vacuum atomic force microscope with freeze–fracture function |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T00%3A29%3A54IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-scitation_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Development%20of%20low-temperature%20and%20high%20vacuum%20atomic%20force%20microscope%20with%20freeze%E2%80%93fracture%20function&rft.jtitle=Review%20of%20scientific%20instruments&rft.au=Nakamoto,%20Keiichi&rft.date=2001-02-01&rft.volume=72&rft.issue=2&rft.spage=1445&rft.epage=1448&rft.pages=1445-1448&rft.issn=0034-6748&rft.eissn=1089-7623&rft.coden=RSINAK&rft_id=info:doi/10.1063/1.1342033&rft_dat=%3Cscitation_cross%3Ersi%3C/scitation_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true |