Regional blood flow measurements from fluorescent microsphere images using an Imaging CryoMicrotome

An automated image acquisition and analysis system has been developed that rapidly determines regional blood flow by using the locations of fluorescent microspheres deposited in tissue. A motor-driven microtome removes sections of frozen tissues in steps variable between 10 and 100 μm. Filtered ligh...

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Veröffentlicht in:Review of scientific instruments 2000-01, Vol.71 (1), p.228-234
Hauptverfasser: Kelly, Jeffrey J., Ewen, Jon R., Bernard, Susan L., Glenny, Robb W., Barlow, Clyde H.
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container_issue 1
container_start_page 228
container_title Review of scientific instruments
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creator Kelly, Jeffrey J.
Ewen, Jon R.
Bernard, Susan L.
Glenny, Robb W.
Barlow, Clyde H.
description An automated image acquisition and analysis system has been developed that rapidly determines regional blood flow by using the locations of fluorescent microspheres deposited in tissue. A motor-driven microtome removes sections of frozen tissues in steps variable between 10 and 100 μm. Filtered light excites microsphere fluorescence from the exposed surface of the remaining tissue block. A charge coupled device camera records fluorescence images from the tissue block surface after removal of each slice. Approximately 450 images are analyzed from perfused rat hearts providing precise x, y, and z locations of about 10 000 microspheres. Image analysis of fluorescent microspheres is much faster and less labor intensive than traditional indirect microsphere-based flow measurements while providing higher quality data.
doi_str_mv 10.1063/1.1150188
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title Regional blood flow measurements from fluorescent microsphere images using an Imaging CryoMicrotome
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