Labeled extracellular vesicles can be found in the blood plasma shortly after intrauterine infusion in bovine
This study explored the migration of follicular fluid (FF)-derived extracellular vesicles (EVs) of the uterine environment to the bloodstream and their interaction with neutrophils and . For the experiment, six Nellore heifers ( ) received an intrauterine infusion seven days after ovulation with 1X...
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| Veröffentlicht in: | Animal reproduction 2024-01, Vol.21 (3), p.e20240064 |
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| creator | Fiorenza, Mariani Farias Bridi, Alessandra Dos Santos, Gislaine Rosa, Paola Maria Alves, Luana Ferst, Juliana Germano Ferraz, Priscila Assis Pugliesi, Guilherme Pohler, Ky Perecin, Felipe Meirelles, Flávio Vieira da Silveira, Juliano Coelho |
| description | This study explored the migration of follicular fluid (FF)-derived extracellular vesicles (EVs) of the uterine environment to the bloodstream and their interaction with neutrophils
and
. For the
experiment, six Nellore heifers (
) received an intrauterine infusion seven days after ovulation with 1X PBS only (sham group; n=1), 1X PBS stained with lipophilic dye PKH26 (control group; n=2), or FF-derived EVs stained with PKH26 (treated group; n=3). Plasma was collected at 0, 10, 30, 60-, 180-, 360-, 720-, and 1440-min post-infusion to obtained EVs for analysis by nano flow cytometry. Labeled EVs were present in the bloodstream at 30- and 60-min post-infusion in the treatment group. Additionally, plasma derived-EVs from all groups were positive for Calcein-AM, Alix, Syntenin, and Calnexin, which confirm the presence of EVs. The second experiment utilized the plasma-derived EVs from the heifers from 30 and 60 min timepoints to evaluate if neutrophils can uptake EVs
. As results, it was possible to observe the presence of labeled EVs in neutrophils treated with plasma derived-EVs from the treatment group. In summary, our results suggest that labeled EVs can migrate from the uterine environment rapidly and interact with circulating immune cells in bovine. |
| doi_str_mv | 10.1590/1984-3143-AR2024-0064 |
| format | Article |
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and
. For the
experiment, six Nellore heifers (
) received an intrauterine infusion seven days after ovulation with 1X PBS only (sham group; n=1), 1X PBS stained with lipophilic dye PKH26 (control group; n=2), or FF-derived EVs stained with PKH26 (treated group; n=3). Plasma was collected at 0, 10, 30, 60-, 180-, 360-, 720-, and 1440-min post-infusion to obtained EVs for analysis by nano flow cytometry. Labeled EVs were present in the bloodstream at 30- and 60-min post-infusion in the treatment group. Additionally, plasma derived-EVs from all groups were positive for Calcein-AM, Alix, Syntenin, and Calnexin, which confirm the presence of EVs. The second experiment utilized the plasma-derived EVs from the heifers from 30 and 60 min timepoints to evaluate if neutrophils can uptake EVs
. As results, it was possible to observe the presence of labeled EVs in neutrophils treated with plasma derived-EVs from the treatment group. In summary, our results suggest that labeled EVs can migrate from the uterine environment rapidly and interact with circulating immune cells in bovine.</description><identifier>ISSN: 1806-9614</identifier><identifier>ISSN: 1984-3143</identifier><identifier>EISSN: 1984-3143</identifier><identifier>DOI: 10.1590/1984-3143-AR2024-0064</identifier><identifier>PMID: 39286366</identifier><language>eng</language><publisher>Brazil: Colégio Brasileiro de Reprodução Animal</publisher><subject>BIOTECHNOLOGY & APPLIED MICROBIOLOGY ; Thematic Section: X International Symposium on Animal Biology of Reproduction (ISABR)</subject><ispartof>Animal reproduction, 2024-01, Vol.21 (3), p.e20240064</ispartof><rights>Copyright © The Author(s).</rights><rights>Copyright © The Author(s). The Author(s).</rights><rights>This work is licensed under a Creative Commons Attribution 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c282t-421edccf53f81c0b5e4acc46183d252074fd0ef75596fef1b1db5323a3a97fc93</cites><orcidid>0000-0001-5739-0677 ; 0000-0001-8202-0678 ; 0000-0002-4693-0196 ; 0000-0002-9888-2245 ; 0000-0002-9129-1047 ; 0000-0003-2009-5863 ; 0000-0002-8004-9343 ; 0000-0003-2429-0893 ; 0000-0003-0372-4920 ; 0000-0002-8972-6590 ; 0000-0002-4796-6393 ; 0000-0001-9486-0121</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11404864/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC11404864/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,27903,27904,53769,53771</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/39286366$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Fiorenza, Mariani Farias</creatorcontrib><creatorcontrib>Bridi, Alessandra</creatorcontrib><creatorcontrib>Dos Santos, Gislaine</creatorcontrib><creatorcontrib>Rosa, Paola Maria</creatorcontrib><creatorcontrib>Alves, Luana</creatorcontrib><creatorcontrib>Ferst, Juliana Germano</creatorcontrib><creatorcontrib>Ferraz, Priscila Assis</creatorcontrib><creatorcontrib>Pugliesi, Guilherme</creatorcontrib><creatorcontrib>Pohler, Ky</creatorcontrib><creatorcontrib>Perecin, Felipe</creatorcontrib><creatorcontrib>Meirelles, Flávio Vieira</creatorcontrib><creatorcontrib>da Silveira, Juliano Coelho</creatorcontrib><title>Labeled extracellular vesicles can be found in the blood plasma shortly after intrauterine infusion in bovine</title><title>Animal reproduction</title><addtitle>Anim Reprod</addtitle><description>This study explored the migration of follicular fluid (FF)-derived extracellular vesicles (EVs) of the uterine environment to the bloodstream and their interaction with neutrophils
and
. For the
experiment, six Nellore heifers (
) received an intrauterine infusion seven days after ovulation with 1X PBS only (sham group; n=1), 1X PBS stained with lipophilic dye PKH26 (control group; n=2), or FF-derived EVs stained with PKH26 (treated group; n=3). Plasma was collected at 0, 10, 30, 60-, 180-, 360-, 720-, and 1440-min post-infusion to obtained EVs for analysis by nano flow cytometry. Labeled EVs were present in the bloodstream at 30- and 60-min post-infusion in the treatment group. Additionally, plasma derived-EVs from all groups were positive for Calcein-AM, Alix, Syntenin, and Calnexin, which confirm the presence of EVs. The second experiment utilized the plasma-derived EVs from the heifers from 30 and 60 min timepoints to evaluate if neutrophils can uptake EVs
. As results, it was possible to observe the presence of labeled EVs in neutrophils treated with plasma derived-EVs from the treatment group. In summary, our results suggest that labeled EVs can migrate from the uterine environment rapidly and interact with circulating immune cells in bovine.</description><subject>BIOTECHNOLOGY & APPLIED MICROBIOLOGY</subject><subject>Thematic Section: X International Symposium on Animal Biology of Reproduction (ISABR)</subject><issn>1806-9614</issn><issn>1984-3143</issn><issn>1984-3143</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2024</creationdate><recordtype>article</recordtype><recordid>eNpVUUtv1DAQthCIlsJPAPnIJWX8iOOcUFWVh7QSEo-z5ThjNpUTL3ayov8eW1tWcPJ4PN_D3xDymsE1a3t4x3otG8GkaG6-cuCyAVDyCbk895-WWoNqesXkBXmR8z2A7HSnnpML0XOthFKXZN7ZAQOOFH-vyToMYQs20SPmyQXM1NmFDkh93JaRTgtd90iHEONID8Hm2dK8j2kND9T6FVOZKCxbqaYFy8VveYpLxQ3xWFovyTNvQ8ZXj-cV-fHh7vvtp2b35ePn25td47jmayM5w9E53wqvmYOhRWmdk4ppMfKWQyf9COi7tu2VR88GNg6t4MIK23fe9eKKXJ94s5swRHMft7QUQfOtxmNqPDUzABAAHYgCeH8CHLZhLtpY_xHMIU2zTQ8m2sn8_7JMe_MzHg1jEqRWsjC8fWRI8deGeTXzlGuedsG45aIJivUd6K6MtqdRl2LOCf1Zh4GpyzVnl8amatTU5Rbcm39NnlF_tyn-AIgCoOs</recordid><startdate>20240101</startdate><enddate>20240101</enddate><creator>Fiorenza, Mariani Farias</creator><creator>Bridi, Alessandra</creator><creator>Dos Santos, Gislaine</creator><creator>Rosa, Paola Maria</creator><creator>Alves, Luana</creator><creator>Ferst, Juliana Germano</creator><creator>Ferraz, Priscila Assis</creator><creator>Pugliesi, Guilherme</creator><creator>Pohler, Ky</creator><creator>Perecin, Felipe</creator><creator>Meirelles, Flávio Vieira</creator><creator>da Silveira, Juliano Coelho</creator><general>Colégio Brasileiro de Reprodução Animal</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope><scope>GPN</scope><orcidid>https://orcid.org/0000-0001-5739-0677</orcidid><orcidid>https://orcid.org/0000-0001-8202-0678</orcidid><orcidid>https://orcid.org/0000-0002-4693-0196</orcidid><orcidid>https://orcid.org/0000-0002-9888-2245</orcidid><orcidid>https://orcid.org/0000-0002-9129-1047</orcidid><orcidid>https://orcid.org/0000-0003-2009-5863</orcidid><orcidid>https://orcid.org/0000-0002-8004-9343</orcidid><orcidid>https://orcid.org/0000-0003-2429-0893</orcidid><orcidid>https://orcid.org/0000-0003-0372-4920</orcidid><orcidid>https://orcid.org/0000-0002-8972-6590</orcidid><orcidid>https://orcid.org/0000-0002-4796-6393</orcidid><orcidid>https://orcid.org/0000-0001-9486-0121</orcidid></search><sort><creationdate>20240101</creationdate><title>Labeled extracellular vesicles can be found in the blood plasma shortly after intrauterine infusion in bovine</title><author>Fiorenza, Mariani Farias ; Bridi, Alessandra ; Dos Santos, Gislaine ; Rosa, Paola Maria ; Alves, Luana ; Ferst, Juliana Germano ; Ferraz, Priscila Assis ; Pugliesi, Guilherme ; Pohler, Ky ; Perecin, Felipe ; Meirelles, Flávio Vieira ; da Silveira, Juliano Coelho</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c282t-421edccf53f81c0b5e4acc46183d252074fd0ef75596fef1b1db5323a3a97fc93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2024</creationdate><topic>BIOTECHNOLOGY & APPLIED MICROBIOLOGY</topic><topic>Thematic Section: X International Symposium on Animal Biology of Reproduction (ISABR)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Fiorenza, Mariani Farias</creatorcontrib><creatorcontrib>Bridi, Alessandra</creatorcontrib><creatorcontrib>Dos Santos, Gislaine</creatorcontrib><creatorcontrib>Rosa, Paola Maria</creatorcontrib><creatorcontrib>Alves, Luana</creatorcontrib><creatorcontrib>Ferst, Juliana Germano</creatorcontrib><creatorcontrib>Ferraz, Priscila Assis</creatorcontrib><creatorcontrib>Pugliesi, Guilherme</creatorcontrib><creatorcontrib>Pohler, Ky</creatorcontrib><creatorcontrib>Perecin, Felipe</creatorcontrib><creatorcontrib>Meirelles, Flávio Vieira</creatorcontrib><creatorcontrib>da Silveira, Juliano Coelho</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SciELO</collection><jtitle>Animal reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Fiorenza, Mariani Farias</au><au>Bridi, Alessandra</au><au>Dos Santos, Gislaine</au><au>Rosa, Paola Maria</au><au>Alves, Luana</au><au>Ferst, Juliana Germano</au><au>Ferraz, Priscila Assis</au><au>Pugliesi, Guilherme</au><au>Pohler, Ky</au><au>Perecin, Felipe</au><au>Meirelles, Flávio Vieira</au><au>da Silveira, Juliano Coelho</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Labeled extracellular vesicles can be found in the blood plasma shortly after intrauterine infusion in bovine</atitle><jtitle>Animal reproduction</jtitle><addtitle>Anim Reprod</addtitle><date>2024-01-01</date><risdate>2024</risdate><volume>21</volume><issue>3</issue><spage>e20240064</spage><pages>e20240064-</pages><issn>1806-9614</issn><issn>1984-3143</issn><eissn>1984-3143</eissn><abstract>This study explored the migration of follicular fluid (FF)-derived extracellular vesicles (EVs) of the uterine environment to the bloodstream and their interaction with neutrophils
and
. For the
experiment, six Nellore heifers (
) received an intrauterine infusion seven days after ovulation with 1X PBS only (sham group; n=1), 1X PBS stained with lipophilic dye PKH26 (control group; n=2), or FF-derived EVs stained with PKH26 (treated group; n=3). Plasma was collected at 0, 10, 30, 60-, 180-, 360-, 720-, and 1440-min post-infusion to obtained EVs for analysis by nano flow cytometry. Labeled EVs were present in the bloodstream at 30- and 60-min post-infusion in the treatment group. Additionally, plasma derived-EVs from all groups were positive for Calcein-AM, Alix, Syntenin, and Calnexin, which confirm the presence of EVs. The second experiment utilized the plasma-derived EVs from the heifers from 30 and 60 min timepoints to evaluate if neutrophils can uptake EVs
. As results, it was possible to observe the presence of labeled EVs in neutrophils treated with plasma derived-EVs from the treatment group. In summary, our results suggest that labeled EVs can migrate from the uterine environment rapidly and interact with circulating immune cells in bovine.</abstract><cop>Brazil</cop><pub>Colégio Brasileiro de Reprodução Animal</pub><pmid>39286366</pmid><doi>10.1590/1984-3143-AR2024-0064</doi><orcidid>https://orcid.org/0000-0001-5739-0677</orcidid><orcidid>https://orcid.org/0000-0001-8202-0678</orcidid><orcidid>https://orcid.org/0000-0002-4693-0196</orcidid><orcidid>https://orcid.org/0000-0002-9888-2245</orcidid><orcidid>https://orcid.org/0000-0002-9129-1047</orcidid><orcidid>https://orcid.org/0000-0003-2009-5863</orcidid><orcidid>https://orcid.org/0000-0002-8004-9343</orcidid><orcidid>https://orcid.org/0000-0003-2429-0893</orcidid><orcidid>https://orcid.org/0000-0003-0372-4920</orcidid><orcidid>https://orcid.org/0000-0002-8972-6590</orcidid><orcidid>https://orcid.org/0000-0002-4796-6393</orcidid><orcidid>https://orcid.org/0000-0001-9486-0121</orcidid><oa>free_for_read</oa></addata></record> |
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| ispartof | Animal reproduction, 2024-01, Vol.21 (3), p.e20240064 |
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| language | eng |
| recordid | cdi_scielo_journals_S1984_31432024000300703 |
| source | DOAJ Directory of Open Access Journals; PubMed Central Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | BIOTECHNOLOGY & APPLIED MICROBIOLOGY Thematic Section: X International Symposium on Animal Biology of Reproduction (ISABR) |
| title | Labeled extracellular vesicles can be found in the blood plasma shortly after intrauterine infusion in bovine |
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