Post-thaw quality of ram sperm frozen with different concentrations of low-density lipoproteins associated with non-enzymatic antioxidants

The cryopreservation reduces ram sperm quality, decreasing the pregnancy rate of ewes inseminated with thawed sperm. Hence, we aimed to improve the post-thaw quality of ram sperm replacing egg yolk on Tris-Glucose extender with different concentrations of LDL (2 or 8%), associated with the addition...

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Veröffentlicht in:Animal reproduction 2023-01, Vol.20 (1), p.e20220068-e20220068
Hauptverfasser: Snoeck, Paola Pereira das Neves, Câmara, Diogo Ribeiro, Moura, Luís Cláudio de Oliveira, da Silva, Maíra Corona, Machado-Neves, Mariana, Teixeira-Neto, Milton Rezende, Henry, Marc
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container_title Animal reproduction
container_volume 20
creator Snoeck, Paola Pereira das Neves
Câmara, Diogo Ribeiro
Moura, Luís Cláudio de Oliveira
da Silva, Maíra Corona
Machado-Neves, Mariana
Teixeira-Neto, Milton Rezende
Henry, Marc
description The cryopreservation reduces ram sperm quality, decreasing the pregnancy rate of ewes inseminated with thawed sperm. Hence, we aimed to improve the post-thaw quality of ram sperm replacing egg yolk on Tris-Glucose extender with different concentrations of LDL (2 or 8%), associated with the addition of 10 mM non-enzymatic antioxidants (ascorbic acid, hydroxytoluene butylate, ascorbyl palmitate, and trehalose). Semen samples were collected from six rams, split into different treatments, and frozen. After thawing, kinematic (CASA), structural (propidium iodide and carboxyfluorescein diacetate) and functional (hypoosmotic test) sperm membrane integrity was assessed. Total motility, VCL, and LIN were also assessed in thawed samples during 3 h of incubation (38 °C). The results showed that hydroxytoluene butylate at 10 mM in Tris-Glucose extender with 8% LDL improved velocity parameters immediately post-thaw compared with Tris-Glucose egg yolk extender, as well as prevented the reduction of total motility and VCL after incubation. There was no benefit of adding ascorbic acid and trehalose. Moreover, for the first time, it was shown the motility impairment promoted by ascorbyl palmitate to ram sperm.
doi_str_mv 10.1590/1984-3143-AR2022-0068
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title Post-thaw quality of ram sperm frozen with different concentrations of low-density lipoproteins associated with non-enzymatic antioxidants
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