Effect of different concentrations of Trolox ® in association with docosahexaenoic acid on equine semen freezing
The aim of this study was to evaluate the association of different concentrations of Trolox and the addition of a fixed concentration of DHA in the freezing of semen of Mangalarga Marchador stallions. To that end, 16 ejaculates were frozen in the following extenders: E1) BotuCrio (BC; Control); E2)...
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| Veröffentlicht in: | Animal reproduction 2022, Vol.19 (4), p.e20220010-e20220010 |
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| container_title | Animal reproduction |
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| creator | Aguiar, Cristiane Silva Barros, Celso Henrique Souza Costa Machado, William Morais Allaman, Ivan Bezerra Leite, Antônio de Oliveira Barbosa, Larissa Pires Snoeck, Paola Pereira das Neves |
| description | The aim of this study was to evaluate the association of different concentrations of Trolox
and the addition of a fixed concentration of DHA in the freezing of semen of Mangalarga Marchador stallions. To that end, 16 ejaculates were frozen in the following extenders: E1) BotuCrio
(BC; Control); E2) BC + 50 ngml
DHA + 30 µM Trolox
(BCDHA30T); E3) BC + 50 ngml
DHA + 40 µM Trolox
(BCDHA40T); E4) BC + 50 ngml
DHA + 50 µM Trolox
(BCDHA50T). All the tested extenders were similar in preserving different kinematic parameters, cell functional integrity, compacted DNA, and high and intermediate mitochondrial activity (P>0.05). However, sperm cryopreserved in BCDHA40T showed higher velocities than sperm frozen in the control extender (P |
| doi_str_mv | 10.1590/1984-3143-AR2022-0010 |
| format | Article |
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and the addition of a fixed concentration of DHA in the freezing of semen of Mangalarga Marchador stallions. To that end, 16 ejaculates were frozen in the following extenders: E1) BotuCrio
(BC; Control); E2) BC + 50 ngml
DHA + 30 µM Trolox
(BCDHA30T); E3) BC + 50 ngml
DHA + 40 µM Trolox
(BCDHA40T); E4) BC + 50 ngml
DHA + 50 µM Trolox
(BCDHA50T). All the tested extenders were similar in preserving different kinematic parameters, cell functional integrity, compacted DNA, and high and intermediate mitochondrial activity (P>0.05). However, sperm cryopreserved in BCDHA40T showed higher velocities than sperm frozen in the control extender (P<0.05). The 30 µM concentration of Trolox
was worse for sperm motility and the 50 µM concentration of Trolox
did not adequately preserve the structural integrity of the membranes in an extender containing DHA when compared to the BotuCrio
(P<0.05) extender. The use of Trolox
in freezing extenders containing DHA did not maximize the effect of BotuCrio
, except for in the case of sperm velocity parameters when at a concentration of 40 µM.</description><identifier>ISSN: 1806-9614</identifier><identifier>ISSN: 1984-3143</identifier><identifier>EISSN: 1984-3143</identifier><identifier>DOI: 10.1590/1984-3143-AR2022-0010</identifier><identifier>PMID: 36504917</identifier><language>eng</language><publisher>Brazil: Colégio Brasileiro de Reprodução Animal</publisher><subject>BIOTECHNOLOGY & APPLIED MICROBIOLOGY</subject><ispartof>Animal reproduction, 2022, Vol.19 (4), p.e20220010-e20220010</ispartof><rights>This work is licensed under a Creative Commons Attribution 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c395t-5b1617ee5cda6581595e18fa9a4a563f0b38d9ad862a5e4e4f152e0192bd5a9a3</citedby><cites>FETCH-LOGICAL-c395t-5b1617ee5cda6581595e18fa9a4a563f0b38d9ad862a5e4e4f152e0192bd5a9a3</cites><orcidid>0000-0003-3440-1593 ; 0000-0002-0134-1478 ; 0000-0002-1473-4991 ; 0000-0002-0207-4121 ; 0000-0003-0883-0466 ; 0000-0003-4445-8630</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,864,885,4023,27922,27923,27924</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/36504917$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Aguiar, Cristiane Silva</creatorcontrib><creatorcontrib>Barros, Celso Henrique Souza Costa</creatorcontrib><creatorcontrib>Machado, William Morais</creatorcontrib><creatorcontrib>Allaman, Ivan Bezerra</creatorcontrib><creatorcontrib>Leite, Antônio de Oliveira</creatorcontrib><creatorcontrib>Barbosa, Larissa Pires</creatorcontrib><creatorcontrib>Snoeck, Paola Pereira das Neves</creatorcontrib><title>Effect of different concentrations of Trolox ® in association with docosahexaenoic acid on equine semen freezing</title><title>Animal reproduction</title><addtitle>Anim Reprod</addtitle><description>The aim of this study was to evaluate the association of different concentrations of Trolox
and the addition of a fixed concentration of DHA in the freezing of semen of Mangalarga Marchador stallions. To that end, 16 ejaculates were frozen in the following extenders: E1) BotuCrio
(BC; Control); E2) BC + 50 ngml
DHA + 30 µM Trolox
(BCDHA30T); E3) BC + 50 ngml
DHA + 40 µM Trolox
(BCDHA40T); E4) BC + 50 ngml
DHA + 50 µM Trolox
(BCDHA50T). All the tested extenders were similar in preserving different kinematic parameters, cell functional integrity, compacted DNA, and high and intermediate mitochondrial activity (P>0.05). However, sperm cryopreserved in BCDHA40T showed higher velocities than sperm frozen in the control extender (P<0.05). The 30 µM concentration of Trolox
was worse for sperm motility and the 50 µM concentration of Trolox
did not adequately preserve the structural integrity of the membranes in an extender containing DHA when compared to the BotuCrio
(P<0.05) extender. The use of Trolox
in freezing extenders containing DHA did not maximize the effect of BotuCrio
, except for in the case of sperm velocity parameters when at a concentration of 40 µM.</description><subject>BIOTECHNOLOGY & APPLIED MICROBIOLOGY</subject><issn>1806-9614</issn><issn>1984-3143</issn><issn>1984-3143</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNo9UUlOxDAQtBCI_QkgH7kE2nHsJEeE2CQkJJaz5XHaYJSxGTsRy6N4BC_DYWBO3XJVudRVhBwwOGaihRPWNlXBWcWL07sSyrIAYLBGtlfv63lvQBatZNUW2UnpBaCqm1puki0uBVQtq7fJ4txaNAMNlnYurxH9QE3wJs-oBxd8mrCHGPrwTr-_qPNUpxSM-wXpmxueaRdMSPoZ3zX64AzVxnU0g7gYnUeacI6e2oj46fzTHtmwuk-4_zd3yePF-cPZVXFze3l9dnpTGN6KoRAzJlmNKEynpWjyzQJZY3WrKy0ktzDjTdfqrpGlFlhhZZkoEVhbzjqRWXyXHC__TcZhH9RLGKPPhup-ikhNEU25QU4FoASeBUdLwWsMixHToOYuGex77TGMSZW14FJyIUWmiiXVxJBSRKteo5vr-KEYqKketfJQOk42aqon6w7_LMbZHLuV6r8P_gMlqIsN</recordid><startdate>2022</startdate><enddate>2022</enddate><creator>Aguiar, Cristiane Silva</creator><creator>Barros, Celso Henrique Souza Costa</creator><creator>Machado, William Morais</creator><creator>Allaman, Ivan Bezerra</creator><creator>Leite, Antônio de Oliveira</creator><creator>Barbosa, Larissa Pires</creator><creator>Snoeck, Paola Pereira das Neves</creator><general>Colégio Brasileiro de Reprodução Animal</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>GPN</scope><orcidid>https://orcid.org/0000-0003-3440-1593</orcidid><orcidid>https://orcid.org/0000-0002-0134-1478</orcidid><orcidid>https://orcid.org/0000-0002-1473-4991</orcidid><orcidid>https://orcid.org/0000-0002-0207-4121</orcidid><orcidid>https://orcid.org/0000-0003-0883-0466</orcidid><orcidid>https://orcid.org/0000-0003-4445-8630</orcidid></search><sort><creationdate>2022</creationdate><title>Effect of different concentrations of Trolox ® in association with docosahexaenoic acid on equine semen freezing</title><author>Aguiar, Cristiane Silva ; Barros, Celso Henrique Souza Costa ; Machado, William Morais ; Allaman, Ivan Bezerra ; Leite, Antônio de Oliveira ; Barbosa, Larissa Pires ; Snoeck, Paola Pereira das Neves</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c395t-5b1617ee5cda6581595e18fa9a4a563f0b38d9ad862a5e4e4f152e0192bd5a9a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><topic>BIOTECHNOLOGY & APPLIED MICROBIOLOGY</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Aguiar, Cristiane Silva</creatorcontrib><creatorcontrib>Barros, Celso Henrique Souza Costa</creatorcontrib><creatorcontrib>Machado, William Morais</creatorcontrib><creatorcontrib>Allaman, Ivan Bezerra</creatorcontrib><creatorcontrib>Leite, Antônio de Oliveira</creatorcontrib><creatorcontrib>Barbosa, Larissa Pires</creatorcontrib><creatorcontrib>Snoeck, Paola Pereira das Neves</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>SciELO</collection><jtitle>Animal reproduction</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Aguiar, Cristiane Silva</au><au>Barros, Celso Henrique Souza Costa</au><au>Machado, William Morais</au><au>Allaman, Ivan Bezerra</au><au>Leite, Antônio de Oliveira</au><au>Barbosa, Larissa Pires</au><au>Snoeck, Paola Pereira das Neves</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of different concentrations of Trolox ® in association with docosahexaenoic acid on equine semen freezing</atitle><jtitle>Animal reproduction</jtitle><addtitle>Anim Reprod</addtitle><date>2022</date><risdate>2022</risdate><volume>19</volume><issue>4</issue><spage>e20220010</spage><epage>e20220010</epage><pages>e20220010-e20220010</pages><issn>1806-9614</issn><issn>1984-3143</issn><eissn>1984-3143</eissn><abstract>The aim of this study was to evaluate the association of different concentrations of Trolox
and the addition of a fixed concentration of DHA in the freezing of semen of Mangalarga Marchador stallions. To that end, 16 ejaculates were frozen in the following extenders: E1) BotuCrio
(BC; Control); E2) BC + 50 ngml
DHA + 30 µM Trolox
(BCDHA30T); E3) BC + 50 ngml
DHA + 40 µM Trolox
(BCDHA40T); E4) BC + 50 ngml
DHA + 50 µM Trolox
(BCDHA50T). All the tested extenders were similar in preserving different kinematic parameters, cell functional integrity, compacted DNA, and high and intermediate mitochondrial activity (P>0.05). However, sperm cryopreserved in BCDHA40T showed higher velocities than sperm frozen in the control extender (P<0.05). The 30 µM concentration of Trolox
was worse for sperm motility and the 50 µM concentration of Trolox
did not adequately preserve the structural integrity of the membranes in an extender containing DHA when compared to the BotuCrio
(P<0.05) extender. The use of Trolox
in freezing extenders containing DHA did not maximize the effect of BotuCrio
, except for in the case of sperm velocity parameters when at a concentration of 40 µM.</abstract><cop>Brazil</cop><pub>Colégio Brasileiro de Reprodução Animal</pub><pmid>36504917</pmid><doi>10.1590/1984-3143-AR2022-0010</doi><orcidid>https://orcid.org/0000-0003-3440-1593</orcidid><orcidid>https://orcid.org/0000-0002-0134-1478</orcidid><orcidid>https://orcid.org/0000-0002-1473-4991</orcidid><orcidid>https://orcid.org/0000-0002-0207-4121</orcidid><orcidid>https://orcid.org/0000-0003-0883-0466</orcidid><orcidid>https://orcid.org/0000-0003-4445-8630</orcidid><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1806-9614 |
| ispartof | Animal reproduction, 2022, Vol.19 (4), p.e20220010-e20220010 |
| issn | 1806-9614 1984-3143 1984-3143 |
| language | eng |
| recordid | cdi_scielo_journals_S1984_31432022000400203 |
| source | DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central Open Access; PubMed Central |
| subjects | BIOTECHNOLOGY & APPLIED MICROBIOLOGY |
| title | Effect of different concentrations of Trolox ® in association with docosahexaenoic acid on equine semen freezing |
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