PROTECTIVE EFFECTS OF ASCORBIC ACID PRETREATMENT IN A RAT MODEL OF INTESTINAL ISCHEMIA-REPERFUSION INJURY: A HISTOMORPHOMETRIC STUDY
Ascorbic acid has shown promise in attenuation of intestinal ischemia-reperfusion (I/R) injury. The aim of this study was to determine the protective effects of ascorbic acid on intestinal morphology during IR injury in rats. We examined morphological changes in the small intestine of Wistar rats af...
Gespeichert in:
| Veröffentlicht in: | Clinics (São Paulo, Brazil) Brazil), 2007-06, Vol.62 (3), p.315-320 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 320 |
|---|---|
| container_issue | 3 |
| container_start_page | 315 |
| container_title | Clinics (São Paulo, Brazil) |
| container_volume | 62 |
| creator | Higa, Oscar Haruo Parra, Edwin Roger Ab'Saber, Alexandre Muxfeldt Farhat, Cecilia Higa, Rita Capelozzi, Vera Luiza |
| description | Ascorbic acid has shown promise in attenuation of intestinal ischemia-reperfusion (I/R) injury. The aim of this study was to determine the protective effects of ascorbic acid on intestinal morphology during IR injury in rats.
We examined morphological changes in the small intestine of Wistar rats after (i) 40 minutes of ischemia (I), (ii) ischemia followed by 30 min of reperfusion (IR), (iii) ischemia with ascorbic acid (IA), (iv) ischemia followed by reperfusion and ascorbic acid (IRA) and (v) in a sham group (S). We used morphometry to evaluate the amount of villous architecture, crypts, necrosis, hemorrhagic infarcts and inflammatory cells at the mesenteric and antimesenteric borders of the small intestine.
Ascorbic acid caused a significant reduction of antimesenteric villous hemorrhagic infarction (p |
| doi_str_mv | 10.1590/S1807-59322007000300017 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_sciel</sourceid><recordid>TN_cdi_scielo_journals_S1807_59322007000300017</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><scielo_id>S1807_59322007000300017</scielo_id><els_id>S1807593222031179</els_id><sourcerecordid>70646511</sourcerecordid><originalsourceid>FETCH-LOGICAL-e306t-b4bef36f483c541b92241347fae13bf58406c1928d1085b7f28da0a29f2abb843</originalsourceid><addsrcrecordid>eNp1kc1v2yAYxlG1af3Y_oWN027uwIA_dmMOXphiE2FSqSdkO1hylcSpnVTqvX94ydKeph0Qj-D3Pu_LAwDfMLrFLEU_KpygOGApCUOEYoQQ8QvHF-AKpwkKmD__4PU7dAmup-nBUymh7BO4xDFL0igmV-BlqZURmZF3Aoo896qCKoe8ypT-JTPIMzmDSy2MFtwUojRQlpBDzQ0s1EwsTrAsjaiMLPkCyiqbi0LyQIul0Pmqkqr0939W-v6nL5vLyqhC6eVcFd7S-1dmNbv_DD529WZyX972G7DKhcnmwUL9lhlfBI6g6BA0tHEdiTqakJZR3KRhSDGhcVc7TJqOJRRFLU7DZI1Rwpq486pGdZh2Yd00CSU34PbsO7W92wz2YTiOO9_Q_o3T_hOnL_h-LtiPw-PRTQe77afWbTb1zg3HycYoohHD2INf38Bjs3Vrux_7bT0-2_egPcDPgPPve-rdaE8z7Fq37kfXHux66C1G9vS5_53mFRqyh1I</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>70646511</pqid></control><display><type>article</type><title>PROTECTIVE EFFECTS OF ASCORBIC ACID PRETREATMENT IN A RAT MODEL OF INTESTINAL ISCHEMIA-REPERFUSION INJURY: A HISTOMORPHOMETRIC STUDY</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>Alma/SFX Local Collection</source><creator>Higa, Oscar Haruo ; Parra, Edwin Roger ; Ab'Saber, Alexandre Muxfeldt ; Farhat, Cecilia ; Higa, Rita ; Capelozzi, Vera Luiza</creator><creatorcontrib>Higa, Oscar Haruo ; Parra, Edwin Roger ; Ab'Saber, Alexandre Muxfeldt ; Farhat, Cecilia ; Higa, Rita ; Capelozzi, Vera Luiza</creatorcontrib><description>Ascorbic acid has shown promise in attenuation of intestinal ischemia-reperfusion (I/R) injury. The aim of this study was to determine the protective effects of ascorbic acid on intestinal morphology during IR injury in rats.
We examined morphological changes in the small intestine of Wistar rats after (i) 40 minutes of ischemia (I), (ii) ischemia followed by 30 min of reperfusion (IR), (iii) ischemia with ascorbic acid (IA), (iv) ischemia followed by reperfusion and ascorbic acid (IRA) and (v) in a sham group (S). We used morphometry to evaluate the amount of villous architecture, crypts, necrosis, hemorrhagic infarcts and inflammatory cells at the mesenteric and antimesenteric borders of the small intestine.
Ascorbic acid caused a significant reduction of antimesenteric villous hemorrhagic infarction (p<0.05) of the small intestine after ischemia followed by reperfusion as well as villous necrosis reduction at both borders after ischemia (p<0.05). The lesions found in the small intestine were more prominent along the antimesenteric margin.
Ascorbic acid pretreatment has a protective effect against the intestinal morphological lesions induced by ischemia-reperfusion injury in rats.
Higa OH, Parra ER, Ab'Saber AM, Farhat C, Higa R, Capelozzi VL. Efeito protetor do pré-tratamento com ácido ascóbico em modelo experimental de isquemia-reperfusão intestinal: um estudo histomorfométrico. Clinics. 2007;62(3):315-20.
O ácido ascórbico tem se mostrado como um agente promissor na atenuação da lesão causada pela isquemia/reperfusão (IR). O objetivo deste estudo foi determinar os efeitos protetores do ácido ascórbico na morfologia intestinal durante a IR em ratos.
Examinamos alterações morfológicas no intestino delgado de ratos do tipo Wistar. Após 40 minutos de isquemia (I), isquemia seguida de reperfusão (IR), isquemia com tratamento com ácido ascórbico (IA), isquemia seguida por 30 minutos de reperfusão e tratamento com ácido ascórbico (IRA) e do grupo sham (S). Utilizamos a morfometria para avaliar quantitativamente a arquitetura dos vilos da mucosa intestinal, criptas intestinais, necrose, hemorragia, células inflamatórias nas bordas mesentéricas e antimesentéricas do intestino delgado.
O ácido ascórbico causou uma redução significativa (p<0,05) no infarto hemorrágico dos vilos intestinais da borda antimesentérica do intestino delgado após isquemia seguida por reperfusão, bem como redução da necrose dos vilos em ambas as bordas após a isquemia (p<0.05). As lesões presentes no intestino delgado foram mais proeminentes na borda antimesentérica.
O pré-tratamento com ácido ascórbico atenuou ou reduziu significativamente as alterações morfológicas no intestino delgado induzidas pela isquemia-reperfusão.</description><identifier>ISSN: 1807-5932</identifier><identifier>ISSN: 1980-5322</identifier><identifier>EISSN: 1980-5322</identifier><identifier>DOI: 10.1590/S1807-59322007000300017</identifier><identifier>PMID: 17589673</identifier><language>eng</language><publisher>Brazil: Elsevier España, S.L.U</publisher><subject>Acido ascórbico ; Animals ; Antioxidants - therapeutic use ; Análise morfométrica ; Ascorbic acid ; Ascorbic Acid - therapeutic use ; Disease Models, Animal ; Intestine, Small - blood supply ; Intestine, Small - drug effects ; Intestine, Small - pathology ; Intestino delgado ; Ischemia ; Ischemia-reperfusion ; Isquemia ; Isquemia reperfusão ; Male ; MEDICINE, GENERAL & INTERNAL ; Morphometric analysis ; Rats ; Rats, Wistar ; Reperfusion Injury - pathology ; Reperfusion Injury - prevention & control ; Small intestine ; Time Factors</subject><ispartof>Clinics (São Paulo, Brazil), 2007-06, Vol.62 (3), p.315-320</ispartof><rights>2007 CLINICS</rights><rights>This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,776,780,860,881,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17589673$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Higa, Oscar Haruo</creatorcontrib><creatorcontrib>Parra, Edwin Roger</creatorcontrib><creatorcontrib>Ab'Saber, Alexandre Muxfeldt</creatorcontrib><creatorcontrib>Farhat, Cecilia</creatorcontrib><creatorcontrib>Higa, Rita</creatorcontrib><creatorcontrib>Capelozzi, Vera Luiza</creatorcontrib><title>PROTECTIVE EFFECTS OF ASCORBIC ACID PRETREATMENT IN A RAT MODEL OF INTESTINAL ISCHEMIA-REPERFUSION INJURY: A HISTOMORPHOMETRIC STUDY</title><title>Clinics (São Paulo, Brazil)</title><addtitle>Clinics (Sao Paulo)</addtitle><description>Ascorbic acid has shown promise in attenuation of intestinal ischemia-reperfusion (I/R) injury. The aim of this study was to determine the protective effects of ascorbic acid on intestinal morphology during IR injury in rats.
We examined morphological changes in the small intestine of Wistar rats after (i) 40 minutes of ischemia (I), (ii) ischemia followed by 30 min of reperfusion (IR), (iii) ischemia with ascorbic acid (IA), (iv) ischemia followed by reperfusion and ascorbic acid (IRA) and (v) in a sham group (S). We used morphometry to evaluate the amount of villous architecture, crypts, necrosis, hemorrhagic infarcts and inflammatory cells at the mesenteric and antimesenteric borders of the small intestine.
Ascorbic acid caused a significant reduction of antimesenteric villous hemorrhagic infarction (p<0.05) of the small intestine after ischemia followed by reperfusion as well as villous necrosis reduction at both borders after ischemia (p<0.05). The lesions found in the small intestine were more prominent along the antimesenteric margin.
Ascorbic acid pretreatment has a protective effect against the intestinal morphological lesions induced by ischemia-reperfusion injury in rats.
Higa OH, Parra ER, Ab'Saber AM, Farhat C, Higa R, Capelozzi VL. Efeito protetor do pré-tratamento com ácido ascóbico em modelo experimental de isquemia-reperfusão intestinal: um estudo histomorfométrico. Clinics. 2007;62(3):315-20.
O ácido ascórbico tem se mostrado como um agente promissor na atenuação da lesão causada pela isquemia/reperfusão (IR). O objetivo deste estudo foi determinar os efeitos protetores do ácido ascórbico na morfologia intestinal durante a IR em ratos.
Examinamos alterações morfológicas no intestino delgado de ratos do tipo Wistar. Após 40 minutos de isquemia (I), isquemia seguida de reperfusão (IR), isquemia com tratamento com ácido ascórbico (IA), isquemia seguida por 30 minutos de reperfusão e tratamento com ácido ascórbico (IRA) e do grupo sham (S). Utilizamos a morfometria para avaliar quantitativamente a arquitetura dos vilos da mucosa intestinal, criptas intestinais, necrose, hemorragia, células inflamatórias nas bordas mesentéricas e antimesentéricas do intestino delgado.
O ácido ascórbico causou uma redução significativa (p<0,05) no infarto hemorrágico dos vilos intestinais da borda antimesentérica do intestino delgado após isquemia seguida por reperfusão, bem como redução da necrose dos vilos em ambas as bordas após a isquemia (p<0.05). As lesões presentes no intestino delgado foram mais proeminentes na borda antimesentérica.
O pré-tratamento com ácido ascórbico atenuou ou reduziu significativamente as alterações morfológicas no intestino delgado induzidas pela isquemia-reperfusão.</description><subject>Acido ascórbico</subject><subject>Animals</subject><subject>Antioxidants - therapeutic use</subject><subject>Análise morfométrica</subject><subject>Ascorbic acid</subject><subject>Ascorbic Acid - therapeutic use</subject><subject>Disease Models, Animal</subject><subject>Intestine, Small - blood supply</subject><subject>Intestine, Small - drug effects</subject><subject>Intestine, Small - pathology</subject><subject>Intestino delgado</subject><subject>Ischemia</subject><subject>Ischemia-reperfusion</subject><subject>Isquemia</subject><subject>Isquemia reperfusão</subject><subject>Male</subject><subject>MEDICINE, GENERAL & INTERNAL</subject><subject>Morphometric analysis</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Reperfusion Injury - pathology</subject><subject>Reperfusion Injury - prevention & control</subject><subject>Small intestine</subject><subject>Time Factors</subject><issn>1807-5932</issn><issn>1980-5322</issn><issn>1980-5322</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2007</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kc1v2yAYxlG1af3Y_oWN027uwIA_dmMOXphiE2FSqSdkO1hylcSpnVTqvX94ydKeph0Qj-D3Pu_LAwDfMLrFLEU_KpygOGApCUOEYoQQ8QvHF-AKpwkKmD__4PU7dAmup-nBUymh7BO4xDFL0igmV-BlqZURmZF3Aoo896qCKoe8ypT-JTPIMzmDSy2MFtwUojRQlpBDzQ0s1EwsTrAsjaiMLPkCyiqbi0LyQIul0Pmqkqr0939W-v6nL5vLyqhC6eVcFd7S-1dmNbv_DD529WZyX972G7DKhcnmwUL9lhlfBI6g6BA0tHEdiTqakJZR3KRhSDGhcVc7TJqOJRRFLU7DZI1Rwpq486pGdZh2Yd00CSU34PbsO7W92wz2YTiOO9_Q_o3T_hOnL_h-LtiPw-PRTQe77afWbTb1zg3HycYoohHD2INf38Bjs3Vrux_7bT0-2_egPcDPgPPve-rdaE8z7Fq37kfXHux66C1G9vS5_53mFRqyh1I</recordid><startdate>20070601</startdate><enddate>20070601</enddate><creator>Higa, Oscar Haruo</creator><creator>Parra, Edwin Roger</creator><creator>Ab'Saber, Alexandre Muxfeldt</creator><creator>Farhat, Cecilia</creator><creator>Higa, Rita</creator><creator>Capelozzi, Vera Luiza</creator><general>Elsevier España, S.L.U</general><general>Faculdade de Medicina / USP</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope><scope>GPN</scope></search><sort><creationdate>20070601</creationdate><title>PROTECTIVE EFFECTS OF ASCORBIC ACID PRETREATMENT IN A RAT MODEL OF INTESTINAL ISCHEMIA-REPERFUSION INJURY: A HISTOMORPHOMETRIC STUDY</title><author>Higa, Oscar Haruo ; Parra, Edwin Roger ; Ab'Saber, Alexandre Muxfeldt ; Farhat, Cecilia ; Higa, Rita ; Capelozzi, Vera Luiza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e306t-b4bef36f483c541b92241347fae13bf58406c1928d1085b7f28da0a29f2abb843</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2007</creationdate><topic>Acido ascórbico</topic><topic>Animals</topic><topic>Antioxidants - therapeutic use</topic><topic>Análise morfométrica</topic><topic>Ascorbic acid</topic><topic>Ascorbic Acid - therapeutic use</topic><topic>Disease Models, Animal</topic><topic>Intestine, Small - blood supply</topic><topic>Intestine, Small - drug effects</topic><topic>Intestine, Small - pathology</topic><topic>Intestino delgado</topic><topic>Ischemia</topic><topic>Ischemia-reperfusion</topic><topic>Isquemia</topic><topic>Isquemia reperfusão</topic><topic>Male</topic><topic>MEDICINE, GENERAL & INTERNAL</topic><topic>Morphometric analysis</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Reperfusion Injury - pathology</topic><topic>Reperfusion Injury - prevention & control</topic><topic>Small intestine</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Higa, Oscar Haruo</creatorcontrib><creatorcontrib>Parra, Edwin Roger</creatorcontrib><creatorcontrib>Ab'Saber, Alexandre Muxfeldt</creatorcontrib><creatorcontrib>Farhat, Cecilia</creatorcontrib><creatorcontrib>Higa, Rita</creatorcontrib><creatorcontrib>Capelozzi, Vera Luiza</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><collection>SciELO</collection><jtitle>Clinics (São Paulo, Brazil)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Higa, Oscar Haruo</au><au>Parra, Edwin Roger</au><au>Ab'Saber, Alexandre Muxfeldt</au><au>Farhat, Cecilia</au><au>Higa, Rita</au><au>Capelozzi, Vera Luiza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PROTECTIVE EFFECTS OF ASCORBIC ACID PRETREATMENT IN A RAT MODEL OF INTESTINAL ISCHEMIA-REPERFUSION INJURY: A HISTOMORPHOMETRIC STUDY</atitle><jtitle>Clinics (São Paulo, Brazil)</jtitle><addtitle>Clinics (Sao Paulo)</addtitle><date>2007-06-01</date><risdate>2007</risdate><volume>62</volume><issue>3</issue><spage>315</spage><epage>320</epage><pages>315-320</pages><issn>1807-5932</issn><issn>1980-5322</issn><eissn>1980-5322</eissn><abstract>Ascorbic acid has shown promise in attenuation of intestinal ischemia-reperfusion (I/R) injury. The aim of this study was to determine the protective effects of ascorbic acid on intestinal morphology during IR injury in rats.
We examined morphological changes in the small intestine of Wistar rats after (i) 40 minutes of ischemia (I), (ii) ischemia followed by 30 min of reperfusion (IR), (iii) ischemia with ascorbic acid (IA), (iv) ischemia followed by reperfusion and ascorbic acid (IRA) and (v) in a sham group (S). We used morphometry to evaluate the amount of villous architecture, crypts, necrosis, hemorrhagic infarcts and inflammatory cells at the mesenteric and antimesenteric borders of the small intestine.
Ascorbic acid caused a significant reduction of antimesenteric villous hemorrhagic infarction (p<0.05) of the small intestine after ischemia followed by reperfusion as well as villous necrosis reduction at both borders after ischemia (p<0.05). The lesions found in the small intestine were more prominent along the antimesenteric margin.
Ascorbic acid pretreatment has a protective effect against the intestinal morphological lesions induced by ischemia-reperfusion injury in rats.
Higa OH, Parra ER, Ab'Saber AM, Farhat C, Higa R, Capelozzi VL. Efeito protetor do pré-tratamento com ácido ascóbico em modelo experimental de isquemia-reperfusão intestinal: um estudo histomorfométrico. Clinics. 2007;62(3):315-20.
O ácido ascórbico tem se mostrado como um agente promissor na atenuação da lesão causada pela isquemia/reperfusão (IR). O objetivo deste estudo foi determinar os efeitos protetores do ácido ascórbico na morfologia intestinal durante a IR em ratos.
Examinamos alterações morfológicas no intestino delgado de ratos do tipo Wistar. Após 40 minutos de isquemia (I), isquemia seguida de reperfusão (IR), isquemia com tratamento com ácido ascórbico (IA), isquemia seguida por 30 minutos de reperfusão e tratamento com ácido ascórbico (IRA) e do grupo sham (S). Utilizamos a morfometria para avaliar quantitativamente a arquitetura dos vilos da mucosa intestinal, criptas intestinais, necrose, hemorragia, células inflamatórias nas bordas mesentéricas e antimesentéricas do intestino delgado.
O ácido ascórbico causou uma redução significativa (p<0,05) no infarto hemorrágico dos vilos intestinais da borda antimesentérica do intestino delgado após isquemia seguida por reperfusão, bem como redução da necrose dos vilos em ambas as bordas após a isquemia (p<0.05). As lesões presentes no intestino delgado foram mais proeminentes na borda antimesentérica.
O pré-tratamento com ácido ascórbico atenuou ou reduziu significativamente as alterações morfológicas no intestino delgado induzidas pela isquemia-reperfusão.</abstract><cop>Brazil</cop><pub>Elsevier España, S.L.U</pub><pmid>17589673</pmid><doi>10.1590/S1807-59322007000300017</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1807-5932 |
| ispartof | Clinics (São Paulo, Brazil), 2007-06, Vol.62 (3), p.315-320 |
| issn | 1807-5932 1980-5322 1980-5322 |
| language | eng |
| recordid | cdi_scielo_journals_S1807_59322007000300017 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Acido ascórbico Animals Antioxidants - therapeutic use Análise morfométrica Ascorbic acid Ascorbic Acid - therapeutic use Disease Models, Animal Intestine, Small - blood supply Intestine, Small - drug effects Intestine, Small - pathology Intestino delgado Ischemia Ischemia-reperfusion Isquemia Isquemia reperfusão Male MEDICINE, GENERAL & INTERNAL Morphometric analysis Rats Rats, Wistar Reperfusion Injury - pathology Reperfusion Injury - prevention & control Small intestine Time Factors |
| title | PROTECTIVE EFFECTS OF ASCORBIC ACID PRETREATMENT IN A RAT MODEL OF INTESTINAL ISCHEMIA-REPERFUSION INJURY: A HISTOMORPHOMETRIC STUDY |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-06T13%3A49%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_sciel&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=PROTECTIVE%20EFFECTS%20OF%20ASCORBIC%20ACID%20PRETREATMENT%20IN%20A%20RAT%20MODEL%20OF%20INTESTINAL%20ISCHEMIA-REPERFUSION%20INJURY:%20A%20HISTOMORPHOMETRIC%20STUDY&rft.jtitle=Clinics%20(S%C3%A3o%20Paulo,%20Brazil)&rft.au=Higa,%20Oscar%20Haruo&rft.date=2007-06-01&rft.volume=62&rft.issue=3&rft.spage=315&rft.epage=320&rft.pages=315-320&rft.issn=1807-5932&rft.eissn=1980-5322&rft_id=info:doi/10.1590/S1807-59322007000300017&rft_dat=%3Cproquest_sciel%3E70646511%3C/proquest_sciel%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=70646511&rft_id=info:pmid/17589673&rft_scielo_id=S1807_59322007000300017&rft_els_id=S1807593222031179&rfr_iscdi=true |