Physico-chemical characterization of collagen scaffolds for tissue engineering
The objective was to research the physical and chemical properties of collagen scaffolds (CS) obtained from bone matrix Nukbone® subject to a demineralization process using hydrochloric acid. The CS samples were characterized by optical and scanning electron microscopy, elemental chemical analysis,...
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| Veröffentlicht in: | Journal of applied research and technology 2016-02, Vol.14 (1), p.77-85 |
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| creator | León-Mancilla, B.H. Araiza-Téllez, M.A. Flores-Flores, J.O. Piña-Barba, M.C. |
| description | The objective was to research the physical and chemical properties of collagen scaffolds (CS) obtained from bone matrix Nukbone® subject to a demineralization process using hydrochloric acid. The CS samples were characterized by optical and scanning electron microscopy, elemental chemical analysis, X-ray diffraction, spectroscopy Infrared, thermal analysis, differential scanning calorimetry and nitrogen adsorption. The microanalysis were used to set the macro- and microstructures of CS. They showed that the CS retained the morphology of Nukbone® with interconnected pores and their size between 100 and 500μm, and it is composed of 20% by weight of HA and the rest is collagen type I. By infrared spectroscopy the functional groups of collagen type I (amide A – 3285, B – 2917, I – 1633, II – 1553 and III – 1239cm−1) were identified. By thermal analysis it was determined that the phenomenon of denaturation of the collagen type I began in the range of 75–85°C and burned above 200°C. |
| doi_str_mv | 10.1016/j.jart.2016.01.001 |
| format | Article |
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The CS samples were characterized by optical and scanning electron microscopy, elemental chemical analysis, X-ray diffraction, spectroscopy Infrared, thermal analysis, differential scanning calorimetry and nitrogen adsorption. The microanalysis were used to set the macro- and microstructures of CS. They showed that the CS retained the morphology of Nukbone® with interconnected pores and their size between 100 and 500μm, and it is composed of 20% by weight of HA and the rest is collagen type I. By infrared spectroscopy the functional groups of collagen type I (amide A – 3285, B – 2917, I – 1633, II – 1553 and III – 1239cm−1) were identified. 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By thermal analysis it was determined that the phenomenon of denaturation of the collagen type I began in the range of 75–85°C and burned above 200°C.</description><subject>Biomaterials</subject><subject>Bone matrix</subject><subject>Collagen scaffolds</subject><subject>Engineering, Electrical & Electronic</subject><subject>Engineering, Multidisciplinary</subject><subject>Tissue engineering</subject><issn>1665-6423</issn><issn>2448-6736</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp9kEtLAzEQgHNQsD7-gKf8gV2T7SabghcpvqCooJ5DOpm0WbYbSbZC_fUm1LOHeRzmm-EbQq45qznj8qavexOnusl9zXjNGD8hMy6lqGTbzM_IeUo9Y1Kpls3Iy9v2kDyECra482AGClsTDUwY_Y-ZfBhpcBTCMJgNjjSBcS4MNlEXIp18SnukOG78iBkYN5fk1Jkh4dVfvSCfD_cfy6dq9fr4vLxbVTCXLa-c6qRzorFr4VpslBJGCiWZsrC2Zm1YzqxbWHQLqRbgGmmcFYLbPN61YOcXpD7uTeBxCLoP-zjmg_q9iOoiWvxZls_RdRlojgDEkFJEp7-i35l40Jzp8jbd6_I2XTDNuM5khm6PEGaVb49Rl3MjoPURYdI2-P_wX8AWeC4</recordid><startdate>20160201</startdate><enddate>20160201</enddate><creator>León-Mancilla, B.H.</creator><creator>Araiza-Téllez, M.A.</creator><creator>Flores-Flores, J.O.</creator><creator>Piña-Barba, M.C.</creator><general>Elsevier España, S.L.U</general><general>UNAM, Centro de Ciencias Aplicadas y Desarrollo Tecnológico</general><scope>6I.</scope><scope>AAFTH</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>GPN</scope></search><sort><creationdate>20160201</creationdate><title>Physico-chemical characterization of collagen scaffolds for tissue engineering</title><author>León-Mancilla, B.H. ; Araiza-Téllez, M.A. ; Flores-Flores, J.O. ; Piña-Barba, M.C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3641-f876ff52db5f4e2885a658608dcbdaba0bda079def9689cf26afd551d5f474cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Biomaterials</topic><topic>Bone matrix</topic><topic>Collagen scaffolds</topic><topic>Engineering, Electrical & Electronic</topic><topic>Engineering, Multidisciplinary</topic><topic>Tissue engineering</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>León-Mancilla, B.H.</creatorcontrib><creatorcontrib>Araiza-Téllez, M.A.</creatorcontrib><creatorcontrib>Flores-Flores, J.O.</creatorcontrib><creatorcontrib>Piña-Barba, M.C.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>CrossRef</collection><collection>SciELO</collection><jtitle>Journal of applied research and technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>León-Mancilla, B.H.</au><au>Araiza-Téllez, M.A.</au><au>Flores-Flores, J.O.</au><au>Piña-Barba, M.C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Physico-chemical characterization of collagen scaffolds for tissue engineering</atitle><jtitle>Journal of applied research and technology</jtitle><addtitle>J. appl. res. technol</addtitle><date>2016-02-01</date><risdate>2016</risdate><volume>14</volume><issue>1</issue><spage>77</spage><epage>85</epage><pages>77-85</pages><issn>1665-6423</issn><issn>2448-6736</issn><abstract>The objective was to research the physical and chemical properties of collagen scaffolds (CS) obtained from bone matrix Nukbone® subject to a demineralization process using hydrochloric acid. 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| subjects | Biomaterials Bone matrix Collagen scaffolds Engineering, Electrical & Electronic Engineering, Multidisciplinary Tissue engineering |
| title | Physico-chemical characterization of collagen scaffolds for tissue engineering |
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