Inactivation of Listeria monocytogenes ATCC 7644 on fresh-cut tomato using nisin in combinations with organic salts
The inhibition of Listeria monocytogenes ATCC 7644 on fresh-cut tomato was investigated using nisin alone, and in combinations with organic salts. Nisin at a concentration of 5000UI/mL was introduced alone or in combination with an organic salt (sodium citrate or sodium acetate each at 3 and 5g/100m...
Gespeichert in:
| Veröffentlicht in: | Brazilian journal of microbiology 2016-07, Vol.47 (3), p.757-763 |
|---|---|
| Hauptverfasser: | , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 763 |
|---|---|
| container_issue | 3 |
| container_start_page | 757 |
| container_title | Brazilian journal of microbiology |
| container_volume | 47 |
| creator | Oladunjoye, Adebola O. Singh, Suren Ijabadeniyi, Oluwatosin A. |
| description | The inhibition of Listeria monocytogenes ATCC 7644 on fresh-cut tomato was investigated using nisin alone, and in combinations with organic salts. Nisin at a concentration of 5000UI/mL was introduced alone or in combination with an organic salt (sodium citrate or sodium acetate each at 3 and 5g/100mL each) on fresh-cut tomato previously inoculated with 108CFU/mL of L. monocytogenes ATCC 7644. Chlorine at 200ppm was used as a control. The inoculated samples were incubated at different temperatures (4, 10 and 25°C) and examined at 0, 24, 48 and 72h. The effects of the antimicrobial treatments on quality parameters of tomato (pH, soluble solids, titratable acidity and vitamin C) were also evaluated, and colour parameters were observed at the lowest storage temperature for 10 days. Both nisin and the organic salts inhibited growth of L. monocytogenes, but the combinations of two compounds were more effective. The nisin–sodium citrate (5%) combination was significantly (p≤0.05) effective, while chlorine was least effective against L. monocytogenes. The quality parameters were substantially retained, especially at 4°C, suggesting good shelf stability at a low temperature. These results substantiate the use of the cheap and eco-friendly approach to reducing this pathogen of health concern in common fresh produce. |
| doi_str_mv | 10.1016/j.bjm.2016.04.027 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_sciel</sourceid><recordid>TN_cdi_scielo_journals_S1517_83822016000300757</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><scielo_id>S1517_83822016000300757</scielo_id><els_id>S1517838216303070</els_id><sourcerecordid>4156808711</sourcerecordid><originalsourceid>FETCH-LOGICAL-c551t-291285a248eec6d78930e2ff7845784b969f28270d7ee25ba79b31edfb08e2773</originalsourceid><addsrcrecordid>eNp9Ul2L1DAULaK4H_oDfJGAL760JmnSpAjCMui6MOCD63NI09uZlDZZk3SW_femO-ugPggJ90LOOffek1sUbwiuCCbNh7HqxrmiOa0wqzAVz4pz0ghZMob585xzIkpZS3pWXMQ4Ykw5ZvRlcUYFbUhGnhfxxmmT7EEn6x3yA9ramCBYjWbvvHlIfgcOIrq63WyQaBhDGTYEiPvSLAklP-vk0RKt2yFnc0D5GD931j1KRnRv0x75sNPOGhT1lOKr4sWgpwivn-Jl8ePL59vN13L77fpmc7UtDecklbQlVHJNmQQwTS9kW2OgwyAk4_l2bdMOVFKBewFAeadF29UE-qHDEqgQ9WVRHXWjsTB5NfoluFxQfV-NUasxq3cY4xpjwVfCpyPhbulm6A24FPSk7oKddXhQXlv194uze7XzB8VaKpq6zQLvnwSC_7lATGq20cA0aQd-iYpiXsvcO2UZ-u4f6Kk9IknNpSCPI5AjygQfY4Dh1AzBat0BNaq8A2qdQ2Gm8g5kzts_pzgxfn96Bnw8AiCbf7AQ1GqQM9DbACap3tv_yP8Ci8DAAw</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1813587177</pqid></control><display><type>article</type><title>Inactivation of Listeria monocytogenes ATCC 7644 on fresh-cut tomato using nisin in combinations with organic salts</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Oladunjoye, Adebola O. ; Singh, Suren ; Ijabadeniyi, Oluwatosin A.</creator><creatorcontrib>Oladunjoye, Adebola O. ; Singh, Suren ; Ijabadeniyi, Oluwatosin A.</creatorcontrib><description>The inhibition of Listeria monocytogenes ATCC 7644 on fresh-cut tomato was investigated using nisin alone, and in combinations with organic salts. Nisin at a concentration of 5000UI/mL was introduced alone or in combination with an organic salt (sodium citrate or sodium acetate each at 3 and 5g/100mL each) on fresh-cut tomato previously inoculated with 108CFU/mL of L. monocytogenes ATCC 7644. Chlorine at 200ppm was used as a control. The inoculated samples were incubated at different temperatures (4, 10 and 25°C) and examined at 0, 24, 48 and 72h. The effects of the antimicrobial treatments on quality parameters of tomato (pH, soluble solids, titratable acidity and vitamin C) were also evaluated, and colour parameters were observed at the lowest storage temperature for 10 days. Both nisin and the organic salts inhibited growth of L. monocytogenes, but the combinations of two compounds were more effective. The nisin–sodium citrate (5%) combination was significantly (p≤0.05) effective, while chlorine was least effective against L. monocytogenes. The quality parameters were substantially retained, especially at 4°C, suggesting good shelf stability at a low temperature. These results substantiate the use of the cheap and eco-friendly approach to reducing this pathogen of health concern in common fresh produce.</description><identifier>ISSN: 1517-8382</identifier><identifier>ISSN: 1678-4405</identifier><identifier>EISSN: 1678-4405</identifier><identifier>DOI: 10.1016/j.bjm.2016.04.027</identifier><identifier>PMID: 27261167</identifier><language>eng</language><publisher>Brazil: Elsevier Editora Ltda</publisher><subject>Anti-Bacterial Agents - pharmacology ; ascorbic acid ; chlorine ; Colony Count, Microbial ; color ; Food Microbiology ; Food Preservation - methods ; Food Preservatives ; Fresh produce ; fresh-cut produce ; Listeria ; Listeria monocytogenes ; Listeria monocytogenes - drug effects ; Listeria monocytogenes - isolation & purification ; Microbial Viability - drug effects ; MICROBIOLOGY ; Nisin ; Nisin - pharmacology ; pathogens ; Quality parameters ; Salt ; Salts - pharmacology ; Sodium acetate ; Sodium citrate ; Solanum lycopersicum - microbiology ; storage temperature ; titratable acidity ; Tomatoes ; total soluble solids</subject><ispartof>Brazilian journal of microbiology, 2016-07, Vol.47 (3), p.757-763</ispartof><rights>2016 Sociedade Brasileira de Microbiologia</rights><rights>Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.</rights><rights>Copyright Sociedade Brasileira de Microbiologia 2016</rights><rights>2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. 2016 Sociedade Brasileira de Microbiologia</rights><rights>This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c551t-291285a248eec6d78930e2ff7845784b969f28270d7ee25ba79b31edfb08e2773</citedby><cites>FETCH-LOGICAL-c551t-291285a248eec6d78930e2ff7845784b969f28270d7ee25ba79b31edfb08e2773</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927639/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927639/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,27901,27902,53766,53768</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/27261167$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oladunjoye, Adebola O.</creatorcontrib><creatorcontrib>Singh, Suren</creatorcontrib><creatorcontrib>Ijabadeniyi, Oluwatosin A.</creatorcontrib><title>Inactivation of Listeria monocytogenes ATCC 7644 on fresh-cut tomato using nisin in combinations with organic salts</title><title>Brazilian journal of microbiology</title><addtitle>Braz J Microbiol</addtitle><description>The inhibition of Listeria monocytogenes ATCC 7644 on fresh-cut tomato was investigated using nisin alone, and in combinations with organic salts. Nisin at a concentration of 5000UI/mL was introduced alone or in combination with an organic salt (sodium citrate or sodium acetate each at 3 and 5g/100mL each) on fresh-cut tomato previously inoculated with 108CFU/mL of L. monocytogenes ATCC 7644. Chlorine at 200ppm was used as a control. The inoculated samples were incubated at different temperatures (4, 10 and 25°C) and examined at 0, 24, 48 and 72h. The effects of the antimicrobial treatments on quality parameters of tomato (pH, soluble solids, titratable acidity and vitamin C) were also evaluated, and colour parameters were observed at the lowest storage temperature for 10 days. Both nisin and the organic salts inhibited growth of L. monocytogenes, but the combinations of two compounds were more effective. The nisin–sodium citrate (5%) combination was significantly (p≤0.05) effective, while chlorine was least effective against L. monocytogenes. The quality parameters were substantially retained, especially at 4°C, suggesting good shelf stability at a low temperature. These results substantiate the use of the cheap and eco-friendly approach to reducing this pathogen of health concern in common fresh produce.</description><subject>Anti-Bacterial Agents - pharmacology</subject><subject>ascorbic acid</subject><subject>chlorine</subject><subject>Colony Count, Microbial</subject><subject>color</subject><subject>Food Microbiology</subject><subject>Food Preservation - methods</subject><subject>Food Preservatives</subject><subject>Fresh produce</subject><subject>fresh-cut produce</subject><subject>Listeria</subject><subject>Listeria monocytogenes</subject><subject>Listeria monocytogenes - drug effects</subject><subject>Listeria monocytogenes - isolation & purification</subject><subject>Microbial Viability - drug effects</subject><subject>MICROBIOLOGY</subject><subject>Nisin</subject><subject>Nisin - pharmacology</subject><subject>pathogens</subject><subject>Quality parameters</subject><subject>Salt</subject><subject>Salts - pharmacology</subject><subject>Sodium acetate</subject><subject>Sodium citrate</subject><subject>Solanum lycopersicum - microbiology</subject><subject>storage temperature</subject><subject>titratable acidity</subject><subject>Tomatoes</subject><subject>total soluble solids</subject><issn>1517-8382</issn><issn>1678-4405</issn><issn>1678-4405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9Ul2L1DAULaK4H_oDfJGAL760JmnSpAjCMui6MOCD63NI09uZlDZZk3SW_femO-ugPggJ90LOOffek1sUbwiuCCbNh7HqxrmiOa0wqzAVz4pz0ghZMob585xzIkpZS3pWXMQ4Ykw5ZvRlcUYFbUhGnhfxxmmT7EEn6x3yA9ramCBYjWbvvHlIfgcOIrq63WyQaBhDGTYEiPvSLAklP-vk0RKt2yFnc0D5GD931j1KRnRv0x75sNPOGhT1lOKr4sWgpwivn-Jl8ePL59vN13L77fpmc7UtDecklbQlVHJNmQQwTS9kW2OgwyAk4_l2bdMOVFKBewFAeadF29UE-qHDEqgQ9WVRHXWjsTB5NfoluFxQfV-NUasxq3cY4xpjwVfCpyPhbulm6A24FPSk7oKddXhQXlv194uze7XzB8VaKpq6zQLvnwSC_7lATGq20cA0aQd-iYpiXsvcO2UZ-u4f6Kk9IknNpSCPI5AjygQfY4Dh1AzBat0BNaq8A2qdQ2Gm8g5kzts_pzgxfn96Bnw8AiCbf7AQ1GqQM9DbACap3tv_yP8Ci8DAAw</recordid><startdate>20160701</startdate><enddate>20160701</enddate><creator>Oladunjoye, Adebola O.</creator><creator>Singh, Suren</creator><creator>Ijabadeniyi, Oluwatosin A.</creator><general>Elsevier Editora Ltda</general><general>Springer Nature B.V</general><general>Elsevier</general><general>Sociedade Brasileira de Microbiologia</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CLZPN</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>PATMY</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PJZUB</scope><scope>PKEHL</scope><scope>PPXIY</scope><scope>PQEST</scope><scope>PQGLB</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>7S9</scope><scope>L.6</scope><scope>5PM</scope><scope>GPN</scope></search><sort><creationdate>20160701</creationdate><title>Inactivation of Listeria monocytogenes ATCC 7644 on fresh-cut tomato using nisin in combinations with organic salts</title><author>Oladunjoye, Adebola O. ; Singh, Suren ; Ijabadeniyi, Oluwatosin A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c551t-291285a248eec6d78930e2ff7845784b969f28270d7ee25ba79b31edfb08e2773</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Anti-Bacterial Agents - pharmacology</topic><topic>ascorbic acid</topic><topic>chlorine</topic><topic>Colony Count, Microbial</topic><topic>color</topic><topic>Food Microbiology</topic><topic>Food Preservation - methods</topic><topic>Food Preservatives</topic><topic>Fresh produce</topic><topic>fresh-cut produce</topic><topic>Listeria</topic><topic>Listeria monocytogenes</topic><topic>Listeria monocytogenes - drug effects</topic><topic>Listeria monocytogenes - isolation & purification</topic><topic>Microbial Viability - drug effects</topic><topic>MICROBIOLOGY</topic><topic>Nisin</topic><topic>Nisin - pharmacology</topic><topic>pathogens</topic><topic>Quality parameters</topic><topic>Salt</topic><topic>Salts - pharmacology</topic><topic>Sodium acetate</topic><topic>Sodium citrate</topic><topic>Solanum lycopersicum - microbiology</topic><topic>storage temperature</topic><topic>titratable acidity</topic><topic>Tomatoes</topic><topic>total soluble solids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oladunjoye, Adebola O.</creatorcontrib><creatorcontrib>Singh, Suren</creatorcontrib><creatorcontrib>Ijabadeniyi, Oluwatosin A.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Latin America & Iberia Database</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Environmental Science Database</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>ProQuest Health & Medical Research Collection</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Health & Nursing</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Applied & Life Sciences</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>AGRICOLA</collection><collection>AGRICOLA - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SciELO</collection><jtitle>Brazilian journal of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oladunjoye, Adebola O.</au><au>Singh, Suren</au><au>Ijabadeniyi, Oluwatosin A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inactivation of Listeria monocytogenes ATCC 7644 on fresh-cut tomato using nisin in combinations with organic salts</atitle><jtitle>Brazilian journal of microbiology</jtitle><addtitle>Braz J Microbiol</addtitle><date>2016-07-01</date><risdate>2016</risdate><volume>47</volume><issue>3</issue><spage>757</spage><epage>763</epage><pages>757-763</pages><issn>1517-8382</issn><issn>1678-4405</issn><eissn>1678-4405</eissn><abstract>The inhibition of Listeria monocytogenes ATCC 7644 on fresh-cut tomato was investigated using nisin alone, and in combinations with organic salts. Nisin at a concentration of 5000UI/mL was introduced alone or in combination with an organic salt (sodium citrate or sodium acetate each at 3 and 5g/100mL each) on fresh-cut tomato previously inoculated with 108CFU/mL of L. monocytogenes ATCC 7644. Chlorine at 200ppm was used as a control. The inoculated samples were incubated at different temperatures (4, 10 and 25°C) and examined at 0, 24, 48 and 72h. The effects of the antimicrobial treatments on quality parameters of tomato (pH, soluble solids, titratable acidity and vitamin C) were also evaluated, and colour parameters were observed at the lowest storage temperature for 10 days. Both nisin and the organic salts inhibited growth of L. monocytogenes, but the combinations of two compounds were more effective. The nisin–sodium citrate (5%) combination was significantly (p≤0.05) effective, while chlorine was least effective against L. monocytogenes. The quality parameters were substantially retained, especially at 4°C, suggesting good shelf stability at a low temperature. These results substantiate the use of the cheap and eco-friendly approach to reducing this pathogen of health concern in common fresh produce.</abstract><cop>Brazil</cop><pub>Elsevier Editora Ltda</pub><pmid>27261167</pmid><doi>10.1016/j.bjm.2016.04.027</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1517-8382 |
| ispartof | Brazilian journal of microbiology, 2016-07, Vol.47 (3), p.757-763 |
| issn | 1517-8382 1678-4405 1678-4405 |
| language | eng |
| recordid | cdi_scielo_journals_S1517_83822016000300757 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Alma/SFX Local Collection |
| subjects | Anti-Bacterial Agents - pharmacology ascorbic acid chlorine Colony Count, Microbial color Food Microbiology Food Preservation - methods Food Preservatives Fresh produce fresh-cut produce Listeria Listeria monocytogenes Listeria monocytogenes - drug effects Listeria monocytogenes - isolation & purification Microbial Viability - drug effects MICROBIOLOGY Nisin Nisin - pharmacology pathogens Quality parameters Salt Salts - pharmacology Sodium acetate Sodium citrate Solanum lycopersicum - microbiology storage temperature titratable acidity Tomatoes total soluble solids |
| title | Inactivation of Listeria monocytogenes ATCC 7644 on fresh-cut tomato using nisin in combinations with organic salts |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T21%3A34%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_sciel&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Inactivation%20of%20Listeria%20monocytogenes%20ATCC%207644%20on%20fresh-cut%20tomato%20using%20nisin%20in%20combinations%20with%20organic%20salts&rft.jtitle=Brazilian%20journal%20of%20microbiology&rft.au=Oladunjoye,%20Adebola%20O.&rft.date=2016-07-01&rft.volume=47&rft.issue=3&rft.spage=757&rft.epage=763&rft.pages=757-763&rft.issn=1517-8382&rft.eissn=1678-4405&rft_id=info:doi/10.1016/j.bjm.2016.04.027&rft_dat=%3Cproquest_sciel%3E4156808711%3C/proquest_sciel%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1813587177&rft_id=info:pmid/27261167&rft_scielo_id=S1517_83822016000300757&rft_els_id=S1517838216303070&rfr_iscdi=true |