Curli fimbria: an Escherichia coli adhesin associated with human cystitis
Escherichia coli is the major causative agent of human cystitis. In this study, a preliminary molecular analysis carried out by PCR (polymerase chain reaction) demonstrated that 100% of 31 E. coli strains isolated from patients with recurrent UTIs (urinary tract infections) showed the presence of th...
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| Veröffentlicht in: | Brazilian journal of microbiology 2016-04, Vol.47 (2), p.414-416 |
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| creator | Cordeiro, Melina Aparecida Werle, Catierine Hirsch Milanez, Guilherme Paier Yano, Tomomasa |
| description | Escherichia coli is the major causative agent of human cystitis. In this study, a preliminary molecular analysis carried out by PCR (polymerase chain reaction) demonstrated that 100% of 31 E. coli strains isolated from patients with recurrent UTIs (urinary tract infections) showed the presence of the curli fimbria gene (csgA). Curli fimbria is known to be associated with bacterial biofilm formation but not with the adhesion of human cystitis-associated E. coli. Therefore, this work aimed to study how curli fimbria is associated with uropathogenic E. coli (UPEC) as an adhesion factor. For this purpose, the csgA gene was deleted from strain UPEC-4, which carries three adhesion factor genes (csgA, fimH and ompA). The wild-type UPEC-4 strain and its mutant (ΔcsgA) were analyzed for their adhesion ability over HTB-9 (human bladder carcinoma), Vero (kidney cells of African green monkey) and HUVEC (human umbilical vein) cells in the presence of α-d-mannose. All the wild-type UPEC strains tested (100%) were able to adhere to all three cell types, while the UPEC-4 ΔcsgA mutant lost its adherence to HTB-9 but continued to adhere to the HUVEC and Vero cells. The results suggest that curli fimbria has an important role in the adhesion processes associated with human UPEC-induced cystitis. |
| doi_str_mv | 10.1016/j.bjm.2016.01.024 |
| format | Article |
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In this study, a preliminary molecular analysis carried out by PCR (polymerase chain reaction) demonstrated that 100% of 31 E. coli strains isolated from patients with recurrent UTIs (urinary tract infections) showed the presence of the curli fimbria gene (csgA). Curli fimbria is known to be associated with bacterial biofilm formation but not with the adhesion of human cystitis-associated E. coli. Therefore, this work aimed to study how curli fimbria is associated with uropathogenic E. coli (UPEC) as an adhesion factor. For this purpose, the csgA gene was deleted from strain UPEC-4, which carries three adhesion factor genes (csgA, fimH and ompA). The wild-type UPEC-4 strain and its mutant (ΔcsgA) were analyzed for their adhesion ability over HTB-9 (human bladder carcinoma), Vero (kidney cells of African green monkey) and HUVEC (human umbilical vein) cells in the presence of α-d-mannose. All the wild-type UPEC strains tested (100%) were able to adhere to all three cell types, while the UPEC-4 ΔcsgA mutant lost its adherence to HTB-9 but continued to adhere to the HUVEC and Vero cells. The results suggest that curli fimbria has an important role in the adhesion processes associated with human UPEC-induced cystitis.</description><identifier>ISSN: 1517-8382</identifier><identifier>ISSN: 1678-4405</identifier><identifier>EISSN: 1678-4405</identifier><identifier>DOI: 10.1016/j.bjm.2016.01.024</identifier><identifier>PMID: 26991275</identifier><language>eng</language><publisher>Brazil: Elsevier Editora Ltda</publisher><subject>Adhesins, Escherichia coli - genetics ; Adhesins, Escherichia coli - metabolism ; Adhesion ; Bacterial Adhesion ; Curli fimbria ; Cystitis - microbiology ; E coli ; Escherichia coli ; Escherichia coli Infections - microbiology ; Escherichia coli Proteins - genetics ; Escherichia coli Proteins - metabolism ; Gene Expression Regulation, Bacterial ; Humans ; Medical Microbiology ; MICROBIOLOGY ; Polymerase chain reaction ; Sequence Deletion ; UPEC ; Urinary tract diseases ; Uropathogenic Escherichia coli - genetics ; Uropathogenic Escherichia coli - metabolism</subject><ispartof>Brazilian journal of microbiology, 2016-04, Vol.47 (2), p.414-416</ispartof><rights>2016 Sociedade Brasileira de Microbiologia</rights><rights>Copyright © 2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. All rights reserved.</rights><rights>Copyright Sociedade Brasileira de Microbiologia 2016</rights><rights>2016 Sociedade Brasileira de Microbiologia. Published by Elsevier Editora Ltda. 2016 Sociedade Brasileira de Microbiologia</rights><rights>This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c518t-f28a576ad1e8701053605602816e8a640433b2f7169c9dadc3ed48e9a723c8c53</citedby><cites>FETCH-LOGICAL-c518t-f28a576ad1e8701053605602816e8a640433b2f7169c9dadc3ed48e9a723c8c53</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4874620/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4874620/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/26991275$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Cordeiro, Melina Aparecida</creatorcontrib><creatorcontrib>Werle, Catierine Hirsch</creatorcontrib><creatorcontrib>Milanez, Guilherme Paier</creatorcontrib><creatorcontrib>Yano, Tomomasa</creatorcontrib><title>Curli fimbria: an Escherichia coli adhesin associated with human cystitis</title><title>Brazilian journal of microbiology</title><addtitle>Braz J Microbiol</addtitle><description>Escherichia coli is the major causative agent of human cystitis. In this study, a preliminary molecular analysis carried out by PCR (polymerase chain reaction) demonstrated that 100% of 31 E. coli strains isolated from patients with recurrent UTIs (urinary tract infections) showed the presence of the curli fimbria gene (csgA). Curli fimbria is known to be associated with bacterial biofilm formation but not with the adhesion of human cystitis-associated E. coli. Therefore, this work aimed to study how curli fimbria is associated with uropathogenic E. coli (UPEC) as an adhesion factor. For this purpose, the csgA gene was deleted from strain UPEC-4, which carries three adhesion factor genes (csgA, fimH and ompA). The wild-type UPEC-4 strain and its mutant (ΔcsgA) were analyzed for their adhesion ability over HTB-9 (human bladder carcinoma), Vero (kidney cells of African green monkey) and HUVEC (human umbilical vein) cells in the presence of α-d-mannose. All the wild-type UPEC strains tested (100%) were able to adhere to all three cell types, while the UPEC-4 ΔcsgA mutant lost its adherence to HTB-9 but continued to adhere to the HUVEC and Vero cells. The results suggest that curli fimbria has an important role in the adhesion processes associated with human UPEC-induced cystitis.</description><subject>Adhesins, Escherichia coli - genetics</subject><subject>Adhesins, Escherichia coli - metabolism</subject><subject>Adhesion</subject><subject>Bacterial Adhesion</subject><subject>Curli fimbria</subject><subject>Cystitis - microbiology</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Escherichia coli Infections - microbiology</subject><subject>Escherichia coli Proteins - genetics</subject><subject>Escherichia coli Proteins - metabolism</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Humans</subject><subject>Medical Microbiology</subject><subject>MICROBIOLOGY</subject><subject>Polymerase chain reaction</subject><subject>Sequence Deletion</subject><subject>UPEC</subject><subject>Urinary tract diseases</subject><subject>Uropathogenic Escherichia coli - genetics</subject><subject>Uropathogenic Escherichia coli - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>SciELO</collection><jtitle>Brazilian journal of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Cordeiro, Melina Aparecida</au><au>Werle, Catierine Hirsch</au><au>Milanez, Guilherme Paier</au><au>Yano, Tomomasa</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Curli fimbria: an Escherichia coli adhesin associated with human cystitis</atitle><jtitle>Brazilian journal of microbiology</jtitle><addtitle>Braz J Microbiol</addtitle><date>2016-04-01</date><risdate>2016</risdate><volume>47</volume><issue>2</issue><spage>414</spage><epage>416</epage><pages>414-416</pages><issn>1517-8382</issn><issn>1678-4405</issn><eissn>1678-4405</eissn><abstract>Escherichia coli is the major causative agent of human cystitis. In this study, a preliminary molecular analysis carried out by PCR (polymerase chain reaction) demonstrated that 100% of 31 E. coli strains isolated from patients with recurrent UTIs (urinary tract infections) showed the presence of the curli fimbria gene (csgA). Curli fimbria is known to be associated with bacterial biofilm formation but not with the adhesion of human cystitis-associated E. coli. Therefore, this work aimed to study how curli fimbria is associated with uropathogenic E. coli (UPEC) as an adhesion factor. For this purpose, the csgA gene was deleted from strain UPEC-4, which carries three adhesion factor genes (csgA, fimH and ompA). The wild-type UPEC-4 strain and its mutant (ΔcsgA) were analyzed for their adhesion ability over HTB-9 (human bladder carcinoma), Vero (kidney cells of African green monkey) and HUVEC (human umbilical vein) cells in the presence of α-d-mannose. All the wild-type UPEC strains tested (100%) were able to adhere to all three cell types, while the UPEC-4 ΔcsgA mutant lost its adherence to HTB-9 but continued to adhere to the HUVEC and Vero cells. The results suggest that curli fimbria has an important role in the adhesion processes associated with human UPEC-induced cystitis.</abstract><cop>Brazil</cop><pub>Elsevier Editora Ltda</pub><pmid>26991275</pmid><doi>10.1016/j.bjm.2016.01.024</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adhesins, Escherichia coli - genetics Adhesins, Escherichia coli - metabolism Adhesion Bacterial Adhesion Curli fimbria Cystitis - microbiology E coli Escherichia coli Escherichia coli Infections - microbiology Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Gene Expression Regulation, Bacterial Humans Medical Microbiology MICROBIOLOGY Polymerase chain reaction Sequence Deletion UPEC Urinary tract diseases Uropathogenic Escherichia coli - genetics Uropathogenic Escherichia coli - metabolism |
| title | Curli fimbria: an Escherichia coli adhesin associated with human cystitis |
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