Comparison of methodologies for conidia production by Alternaria alternata from citrus

Conidia production is a problem in the study of Alternaria alternata from citrus. Thus, this study aimed to compare existing methodologies for conidial production of A. alternata isolated from Ponkan tangerine (2 isolates), Cravo lemon (1 isolate), Pêra orange (2 isolates) and Murcott tangor (1 isol...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Brazilian journal of microbiology 2008-12, Vol.39 (4), p.792-798
Hauptverfasser: Carvalho, Daniel D. C., Alves, Eduardo, Batista, Tereza R. S., Camargos, Renato B., Lopes, Eloísa A. G. L.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 798
container_issue 4
container_start_page 792
container_title Brazilian journal of microbiology
container_volume 39
creator Carvalho, Daniel D. C.
Alves, Eduardo
Batista, Tereza R. S.
Camargos, Renato B.
Lopes, Eloísa A. G. L.
description Conidia production is a problem in the study of Alternaria alternata from citrus. Thus, this study aimed to compare existing methodologies for conidial production of A. alternata isolated from Ponkan tangerine (2 isolates), Cravo lemon (1 isolate), Pêra orange (2 isolates) and Murcott tangor (1 isolate). The methodologies used were conidia production with 12 and 24 hours under white fluorescent light, evaluation with 24 and 48 hours after applying fungal mycelium stress technique, cold stress followed by injury of mycelium and evaluation with 24 hours, using healthy vegetable tissue and the use of black fluorescent near ultraviolet (NUV) lamp. Satisfactory result was obtained with A. alternata isolate from Murcott tangor, with the production of 2.8 x 105 conidia mL-1, when fungal mycelium was stressed (Petri dish with 66.66% of fungi growth) and subsequently 24 h of growth. The use of white light (24 h) and black fluorescent NUV lamp also induced expressive conidia production by one isolate of Ponkan tangerine, which produced 17.2 x 105 and 10.1 x 105 conidia mL-1 and another of Murcott tangor, which produced 13.9 x 105 and 10.1 x 105 conidia mL-1, respectively. The remaining methodologies analyzed in this study were not able to induce conidia production in satisfactory quantity. The use of both mycelium stress technique and white light (24 h) and black fluorescent NUV lamp allowed the production of enough quantities of conidia to be used in vitro (detection of fungitoxic substances)and in vivo (pathogenicity test)assays, respectively. [PUBLICATION ABSTRACT]
doi_str_mv 10.1590/S1517-83822008000400036
format Article
fullrecord <record><control><sourceid>proquest_sciel</sourceid><recordid>TN_cdi_scielo_journals_S1517_83822008000400036</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><scielo_id>S1517_83822008000400036</scielo_id><sourcerecordid>20605247</sourcerecordid><originalsourceid>FETCH-LOGICAL-c404t-ef47c9b537407c579a2e98a545cff94714393a36e69059f1d92bbfd75f9acd5c3</originalsourceid><addsrcrecordid>eNp1kU1LxDAQhoMouH78BgOCt66TNGma47L4BQse_LiGNE00S9usSXvYf2901YPiYZj38LzDOzMInRGYEy7h8oFwIoq6rCkFqAGA5SqrPTQjlagLxoDvZ_0NHaKjlNYAlAOjM_S8DP1GR5_CgIPDvR1fQxu68OJtwi5EbMLgW6_xJoZ2MqPPXLPFi260ccg-jfVOjhq7GHps_BindIIOnO6SPf3qx-jp-upxeVus7m_ulotVYRiwsbCOCSMbXgoGwnAhNbWy1pxx45xkgrBSlrqsbCWBS0daSZvGtYI7qU3LTXmM5ru5yXjbBbUOU87SJfV5FfXnKtlwsTPkfd4mm0bV-2Rs1-nBhikpChVwykQGz3-BP7MJreuaMsllpsSOMjGkFK1Tm-h7HbeKgPp4z79B3gE7goCG</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1288824959</pqid></control><display><type>article</type><title>Comparison of methodologies for conidia production by Alternaria alternata from citrus</title><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><creator>Carvalho, Daniel D. C. ; Alves, Eduardo ; Batista, Tereza R. S. ; Camargos, Renato B. ; Lopes, Eloísa A. G. L.</creator><creatorcontrib>Carvalho, Daniel D. C. ; Alves, Eduardo ; Batista, Tereza R. S. ; Camargos, Renato B. ; Lopes, Eloísa A. G. L.</creatorcontrib><description>Conidia production is a problem in the study of Alternaria alternata from citrus. Thus, this study aimed to compare existing methodologies for conidial production of A. alternata isolated from Ponkan tangerine (2 isolates), Cravo lemon (1 isolate), Pêra orange (2 isolates) and Murcott tangor (1 isolate). The methodologies used were conidia production with 12 and 24 hours under white fluorescent light, evaluation with 24 and 48 hours after applying fungal mycelium stress technique, cold stress followed by injury of mycelium and evaluation with 24 hours, using healthy vegetable tissue and the use of black fluorescent near ultraviolet (NUV) lamp. Satisfactory result was obtained with A. alternata isolate from Murcott tangor, with the production of 2.8 x 105 conidia mL-1, when fungal mycelium was stressed (Petri dish with 66.66% of fungi growth) and subsequently 24 h of growth. The use of white light (24 h) and black fluorescent NUV lamp also induced expressive conidia production by one isolate of Ponkan tangerine, which produced 17.2 x 105 and 10.1 x 105 conidia mL-1 and another of Murcott tangor, which produced 13.9 x 105 and 10.1 x 105 conidia mL-1, respectively. The remaining methodologies analyzed in this study were not able to induce conidia production in satisfactory quantity. The use of both mycelium stress technique and white light (24 h) and black fluorescent NUV lamp allowed the production of enough quantities of conidia to be used in vitro (detection of fungitoxic substances)and in vivo (pathogenicity test)assays, respectively. [PUBLICATION ABSTRACT]</description><identifier>ISSN: 1517-8382</identifier><identifier>ISSN: 1678-4405</identifier><identifier>EISSN: 1678-4405</identifier><identifier>DOI: 10.1590/S1517-83822008000400036</identifier><language>eng</language><publisher>São Paulo: Springer Nature B.V</publisher><subject>Alternaria alternata ; Citrus ; Citrus fruits ; Crop diseases ; MICROBIOLOGY ; Plant pathology</subject><ispartof>Brazilian journal of microbiology, 2008-12, Vol.39 (4), p.792-798</ispartof><rights>Copyright Sociedade Brasileira de Microbiologia 2008</rights><rights>This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c404t-ef47c9b537407c579a2e98a545cff94714393a36e69059f1d92bbfd75f9acd5c3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids></links><search><creatorcontrib>Carvalho, Daniel D. C.</creatorcontrib><creatorcontrib>Alves, Eduardo</creatorcontrib><creatorcontrib>Batista, Tereza R. S.</creatorcontrib><creatorcontrib>Camargos, Renato B.</creatorcontrib><creatorcontrib>Lopes, Eloísa A. G. L.</creatorcontrib><title>Comparison of methodologies for conidia production by Alternaria alternata from citrus</title><title>Brazilian journal of microbiology</title><addtitle>Braz. J. Microbiol</addtitle><description>Conidia production is a problem in the study of Alternaria alternata from citrus. Thus, this study aimed to compare existing methodologies for conidial production of A. alternata isolated from Ponkan tangerine (2 isolates), Cravo lemon (1 isolate), Pêra orange (2 isolates) and Murcott tangor (1 isolate). The methodologies used were conidia production with 12 and 24 hours under white fluorescent light, evaluation with 24 and 48 hours after applying fungal mycelium stress technique, cold stress followed by injury of mycelium and evaluation with 24 hours, using healthy vegetable tissue and the use of black fluorescent near ultraviolet (NUV) lamp. Satisfactory result was obtained with A. alternata isolate from Murcott tangor, with the production of 2.8 x 105 conidia mL-1, when fungal mycelium was stressed (Petri dish with 66.66% of fungi growth) and subsequently 24 h of growth. The use of white light (24 h) and black fluorescent NUV lamp also induced expressive conidia production by one isolate of Ponkan tangerine, which produced 17.2 x 105 and 10.1 x 105 conidia mL-1 and another of Murcott tangor, which produced 13.9 x 105 and 10.1 x 105 conidia mL-1, respectively. The remaining methodologies analyzed in this study were not able to induce conidia production in satisfactory quantity. The use of both mycelium stress technique and white light (24 h) and black fluorescent NUV lamp allowed the production of enough quantities of conidia to be used in vitro (detection of fungitoxic substances)and in vivo (pathogenicity test)assays, respectively. [PUBLICATION ABSTRACT]</description><subject>Alternaria alternata</subject><subject>Citrus</subject><subject>Citrus fruits</subject><subject>Crop diseases</subject><subject>MICROBIOLOGY</subject><subject>Plant pathology</subject><issn>1517-8382</issn><issn>1678-4405</issn><issn>1678-4405</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNp1kU1LxDAQhoMouH78BgOCt66TNGma47L4BQse_LiGNE00S9usSXvYf2901YPiYZj38LzDOzMInRGYEy7h8oFwIoq6rCkFqAGA5SqrPTQjlagLxoDvZ_0NHaKjlNYAlAOjM_S8DP1GR5_CgIPDvR1fQxu68OJtwi5EbMLgW6_xJoZ2MqPPXLPFi260ccg-jfVOjhq7GHps_BindIIOnO6SPf3qx-jp-upxeVus7m_ulotVYRiwsbCOCSMbXgoGwnAhNbWy1pxx45xkgrBSlrqsbCWBS0daSZvGtYI7qU3LTXmM5ru5yXjbBbUOU87SJfV5FfXnKtlwsTPkfd4mm0bV-2Rs1-nBhikpChVwykQGz3-BP7MJreuaMsllpsSOMjGkFK1Tm-h7HbeKgPp4z79B3gE7goCG</recordid><startdate>200812</startdate><enddate>200812</enddate><creator>Carvalho, Daniel D. C.</creator><creator>Alves, Eduardo</creator><creator>Batista, Tereza R. S.</creator><creator>Camargos, Renato B.</creator><creator>Lopes, Eloísa A. G. L.</creator><general>Springer Nature B.V</general><general>Sociedade Brasileira de Microbiologia</general><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>CLZPN</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>PATMY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PYCSY</scope><scope>Q9U</scope><scope>GPN</scope></search><sort><creationdate>200812</creationdate><title>Comparison of methodologies for conidia production by Alternaria alternata from citrus</title><author>Carvalho, Daniel D. C. ; Alves, Eduardo ; Batista, Tereza R. S. ; Camargos, Renato B. ; Lopes, Eloísa A. G. L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c404t-ef47c9b537407c579a2e98a545cff94714393a36e69059f1d92bbfd75f9acd5c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Alternaria alternata</topic><topic>Citrus</topic><topic>Citrus fruits</topic><topic>Crop diseases</topic><topic>MICROBIOLOGY</topic><topic>Plant pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Carvalho, Daniel D. C.</creatorcontrib><creatorcontrib>Alves, Eduardo</creatorcontrib><creatorcontrib>Batista, Tereza R. S.</creatorcontrib><creatorcontrib>Camargos, Renato B.</creatorcontrib><creatorcontrib>Lopes, Eloísa A. G. L.</creatorcontrib><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural &amp; Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>Latin America &amp; Iberia Database</collection><collection>ProQuest Central Korea</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Science Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Environmental Science Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Environmental Science Collection</collection><collection>ProQuest Central Basic</collection><collection>SciELO</collection><jtitle>Brazilian journal of microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Carvalho, Daniel D. C.</au><au>Alves, Eduardo</au><au>Batista, Tereza R. S.</au><au>Camargos, Renato B.</au><au>Lopes, Eloísa A. G. L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of methodologies for conidia production by Alternaria alternata from citrus</atitle><jtitle>Brazilian journal of microbiology</jtitle><addtitle>Braz. J. Microbiol</addtitle><date>2008-12</date><risdate>2008</risdate><volume>39</volume><issue>4</issue><spage>792</spage><epage>798</epage><pages>792-798</pages><issn>1517-8382</issn><issn>1678-4405</issn><eissn>1678-4405</eissn><abstract>Conidia production is a problem in the study of Alternaria alternata from citrus. Thus, this study aimed to compare existing methodologies for conidial production of A. alternata isolated from Ponkan tangerine (2 isolates), Cravo lemon (1 isolate), Pêra orange (2 isolates) and Murcott tangor (1 isolate). The methodologies used were conidia production with 12 and 24 hours under white fluorescent light, evaluation with 24 and 48 hours after applying fungal mycelium stress technique, cold stress followed by injury of mycelium and evaluation with 24 hours, using healthy vegetable tissue and the use of black fluorescent near ultraviolet (NUV) lamp. Satisfactory result was obtained with A. alternata isolate from Murcott tangor, with the production of 2.8 x 105 conidia mL-1, when fungal mycelium was stressed (Petri dish with 66.66% of fungi growth) and subsequently 24 h of growth. The use of white light (24 h) and black fluorescent NUV lamp also induced expressive conidia production by one isolate of Ponkan tangerine, which produced 17.2 x 105 and 10.1 x 105 conidia mL-1 and another of Murcott tangor, which produced 13.9 x 105 and 10.1 x 105 conidia mL-1, respectively. The remaining methodologies analyzed in this study were not able to induce conidia production in satisfactory quantity. The use of both mycelium stress technique and white light (24 h) and black fluorescent NUV lamp allowed the production of enough quantities of conidia to be used in vitro (detection of fungitoxic substances)and in vivo (pathogenicity test)assays, respectively. [PUBLICATION ABSTRACT]</abstract><cop>São Paulo</cop><pub>Springer Nature B.V</pub><doi>10.1590/S1517-83822008000400036</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1517-8382
ispartof Brazilian journal of microbiology, 2008-12, Vol.39 (4), p.792-798
issn 1517-8382
1678-4405
1678-4405
language eng
recordid cdi_scielo_journals_S1517_83822008000400036
source EZB-FREE-00999 freely available EZB journals; PubMed Central; Alma/SFX Local Collection
subjects Alternaria alternata
Citrus
Citrus fruits
Crop diseases
MICROBIOLOGY
Plant pathology
title Comparison of methodologies for conidia production by Alternaria alternata from citrus
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-07T21%3A56%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_sciel&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparison%20of%20methodologies%20for%20conidia%20production%20by%20Alternaria%20alternata%20from%20citrus&rft.jtitle=Brazilian%20journal%20of%20microbiology&rft.au=Carvalho,%20Daniel%20D.%20C.&rft.date=2008-12&rft.volume=39&rft.issue=4&rft.spage=792&rft.epage=798&rft.pages=792-798&rft.issn=1517-8382&rft.eissn=1678-4405&rft_id=info:doi/10.1590/S1517-83822008000400036&rft_dat=%3Cproquest_sciel%3E20605247%3C/proquest_sciel%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1288824959&rft_id=info:pmid/&rft_scielo_id=S1517_83822008000400036&rfr_iscdi=true