Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008
To describe the virological characteristics of the influenza strains circulating in Argentina in 2005-2008 and to assess the prevalence of antiviral resistance. On the basis of their geographical spread and prevalence, influenza A and B isolates grown in Madin-Darby canine kidney cells were selected...
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| Veröffentlicht in: | Revista panamericana de salud pública 2011-12, Vol.30 (6), p.634-640 |
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| description | To describe the virological characteristics of the influenza strains circulating in Argentina in 2005-2008 and to assess the prevalence of antiviral resistance.
On the basis of their geographical spread and prevalence, influenza A and B isolates grown in Madin-Darby canine kidney cells were selected after antigenic and genomic characterization to be analyzed for antiviral resistance by enzymatic assay and pyrosequencing. Amantadine susceptibility was evaluated by pyrosequencing for known resistance markers on 45 strains of influenza A. Susceptibility to oseltamivir and zanamivir was evaluated by enzymatic assay of 67 influenza A and 46 influenza B strains, some of which were further analyzed by sequencing the neuraminidase gene.
Resistance to amantadine was observed only on A(H3N2) strains (29/33); all of them carried the mutation S31N in their M2 sequence. Oseltamivir resistance was observed in 12 (34.3%) of the 35 A(H1N1) strains from 2008; all of them carried the mutation H275Y in their neuraminidase sequence. All these viruses remained sensitive to zanamivir.
This study describes a high incidence of amantadine-resistant influenza A(H3N2) viruses since 2006 and an unprecedented increase in oseltamivir resistance detected only in influenza A(H1N1) viruses isolated in 2008. Influenza A and B viruses were more sensitive to oseltamivir than to zanamivir, and influenza A viruses were more sensitive to both neuraminidase inhibitors than the influenza B viruses. The national data generated and analyzed in this study may help increase knowledge about influenza antiviral drug resistance, which is a problem of global concern. |
| doi_str_mv | 10.1590/S1020-49892011001200023 |
| format | Article |
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On the basis of their geographical spread and prevalence, influenza A and B isolates grown in Madin-Darby canine kidney cells were selected after antigenic and genomic characterization to be analyzed for antiviral resistance by enzymatic assay and pyrosequencing. Amantadine susceptibility was evaluated by pyrosequencing for known resistance markers on 45 strains of influenza A. Susceptibility to oseltamivir and zanamivir was evaluated by enzymatic assay of 67 influenza A and 46 influenza B strains, some of which were further analyzed by sequencing the neuraminidase gene.
Resistance to amantadine was observed only on A(H3N2) strains (29/33); all of them carried the mutation S31N in their M2 sequence. Oseltamivir resistance was observed in 12 (34.3%) of the 35 A(H1N1) strains from 2008; all of them carried the mutation H275Y in their neuraminidase sequence. All these viruses remained sensitive to zanamivir.
This study describes a high incidence of amantadine-resistant influenza A(H3N2) viruses since 2006 and an unprecedented increase in oseltamivir resistance detected only in influenza A(H1N1) viruses isolated in 2008. Influenza A and B viruses were more sensitive to oseltamivir than to zanamivir, and influenza A viruses were more sensitive to both neuraminidase inhibitors than the influenza B viruses. The national data generated and analyzed in this study may help increase knowledge about influenza antiviral drug resistance, which is a problem of global concern.</description><identifier>ISSN: 1020-4989</identifier><identifier>ISSN: 1680-5348</identifier><identifier>EISSN: 1680-5348</identifier><identifier>DOI: 10.1590/S1020-49892011001200023</identifier><identifier>PMID: 22358415</identifier><language>eng</language><publisher>United States: Organización Panamericana de la Salud</publisher><subject>Amantadine - pharmacology ; Animals ; Antiviral Agents - pharmacology ; Argentina ; Argentina - epidemiology ; Cell Line ; Data analysis ; Dogs ; Drug Resistance, Multiple, Viral - genetics ; Drug Resistance, Viral ; Drug use ; Epidemiology ; Health Policy & Services ; Hospitals ; Humans ; Influenza A virus - drug effects ; Influenza A virus - genetics ; Influenza A virus - isolation & purification ; Influenza A Virus, H1N1 Subtype - drug effects ; Influenza A Virus, H1N1 Subtype - genetics ; Influenza B virus - drug effects ; Influenza B virus - genetics ; Influenza B virus - isolation & purification ; Influenza, Human - epidemiology ; Influenza, Human - virology ; Medical research ; Medical treatment ; Morbidity - trends ; Mutation, Missense ; Neuraminidase - antagonists & inhibitors ; Neuraminidase - genetics ; Oseltamivir - pharmacology ; Point Mutation ; Population Surveillance ; Public health ; Resistance ; Seasons ; Virus Cultivation ; Zanamivir - pharmacology</subject><ispartof>Revista panamericana de salud pública, 2011-12, Vol.30 (6), p.634-640</ispartof><rights>This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c433t-e81f908fafb3c403f0a1ea98493f0d5d14830296433410346744dd3e20d80e183</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22358415$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pontoriero, Andrea</creatorcontrib><creatorcontrib>Baumeister, Elsa</creatorcontrib><creatorcontrib>Campos, Ana M</creatorcontrib><creatorcontrib>Savy, Vilma L</creatorcontrib><title>Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008</title><title>Revista panamericana de salud pública</title><addtitle>Rev Panam Salud Publica</addtitle><description>To describe the virological characteristics of the influenza strains circulating in Argentina in 2005-2008 and to assess the prevalence of antiviral resistance.
On the basis of their geographical spread and prevalence, influenza A and B isolates grown in Madin-Darby canine kidney cells were selected after antigenic and genomic characterization to be analyzed for antiviral resistance by enzymatic assay and pyrosequencing. Amantadine susceptibility was evaluated by pyrosequencing for known resistance markers on 45 strains of influenza A. Susceptibility to oseltamivir and zanamivir was evaluated by enzymatic assay of 67 influenza A and 46 influenza B strains, some of which were further analyzed by sequencing the neuraminidase gene.
Resistance to amantadine was observed only on A(H3N2) strains (29/33); all of them carried the mutation S31N in their M2 sequence. Oseltamivir resistance was observed in 12 (34.3%) of the 35 A(H1N1) strains from 2008; all of them carried the mutation H275Y in their neuraminidase sequence. All these viruses remained sensitive to zanamivir.
This study describes a high incidence of amantadine-resistant influenza A(H3N2) viruses since 2006 and an unprecedented increase in oseltamivir resistance detected only in influenza A(H1N1) viruses isolated in 2008. Influenza A and B viruses were more sensitive to oseltamivir than to zanamivir, and influenza A viruses were more sensitive to both neuraminidase inhibitors than the influenza B viruses. The national data generated and analyzed in this study may help increase knowledge about influenza antiviral drug resistance, which is a problem of global concern.</description><subject>Amantadine - pharmacology</subject><subject>Animals</subject><subject>Antiviral Agents - pharmacology</subject><subject>Argentina</subject><subject>Argentina - epidemiology</subject><subject>Cell Line</subject><subject>Data analysis</subject><subject>Dogs</subject><subject>Drug Resistance, Multiple, Viral - genetics</subject><subject>Drug Resistance, Viral</subject><subject>Drug use</subject><subject>Epidemiology</subject><subject>Health Policy & Services</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Influenza A virus - drug effects</subject><subject>Influenza A virus - genetics</subject><subject>Influenza A virus - isolation & purification</subject><subject>Influenza A Virus, H1N1 Subtype - drug effects</subject><subject>Influenza A Virus, H1N1 Subtype - genetics</subject><subject>Influenza B virus - drug effects</subject><subject>Influenza B virus - genetics</subject><subject>Influenza B virus - isolation & purification</subject><subject>Influenza, Human - epidemiology</subject><subject>Influenza, Human - virology</subject><subject>Medical research</subject><subject>Medical treatment</subject><subject>Morbidity - trends</subject><subject>Mutation, Missense</subject><subject>Neuraminidase - antagonists & inhibitors</subject><subject>Neuraminidase - genetics</subject><subject>Oseltamivir - pharmacology</subject><subject>Point Mutation</subject><subject>Population Surveillance</subject><subject>Public health</subject><subject>Resistance</subject><subject>Seasons</subject><subject>Virus Cultivation</subject><subject>Zanamivir - pharmacology</subject><issn>1020-4989</issn><issn>1680-5348</issn><issn>1680-5348</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kctOwzAQRS0EoqXwC5AdLEgZx05qL6uKl1SJBY9t5CZ2cZXExU4qwdczfbFB6sL2WHPuzPiakCsKQ5pKuHulkEDMpZAJUApAEwBI2BHp00xAnDIujjHeQz1yFsICCZoxekp6ScJSwWnaJ7MP613l5rZQVRQ6v9K2qlRT6Eg1Ja7WrqzHlNfBhnaTcCb67GrVRLYxVaebHxUh0wW8R2M_16hp1G2EE6UxbuKcnBhVBX2xOwfk_eH-bfIUT18enyfjaVxwxtpYC2okCKPMjBUcmAFFtZKCSwzLtKRcMEhkhjCnwHg24rwsmU6gFKCpYAMy3NYNhdWVyxeu8w02zDdm5f_MQsH1VrD07qvToc1rGwq9fr92XcglujRCJ9elbw6SOI-QgNZniI62aOFdCF6bfOltrfw3Qvn67w6Mc7lr0s1qXf7p9p_FfgG1QI97</recordid><startdate>201112</startdate><enddate>201112</enddate><creator>Pontoriero, Andrea</creator><creator>Baumeister, Elsa</creator><creator>Campos, Ana M</creator><creator>Savy, Vilma L</creator><general>Organización Panamericana de la Salud</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8BJ</scope><scope>FQK</scope><scope>JBE</scope><scope>7X8</scope><scope>GPN</scope></search><sort><creationdate>201112</creationdate><title>Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008</title><author>Pontoriero, Andrea ; Baumeister, Elsa ; Campos, Ana M ; Savy, Vilma L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c433t-e81f908fafb3c403f0a1ea98493f0d5d14830296433410346744dd3e20d80e183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Amantadine - pharmacology</topic><topic>Animals</topic><topic>Antiviral Agents - pharmacology</topic><topic>Argentina</topic><topic>Argentina - epidemiology</topic><topic>Cell Line</topic><topic>Data analysis</topic><topic>Dogs</topic><topic>Drug Resistance, Multiple, Viral - genetics</topic><topic>Drug Resistance, Viral</topic><topic>Drug use</topic><topic>Epidemiology</topic><topic>Health Policy & Services</topic><topic>Hospitals</topic><topic>Humans</topic><topic>Influenza A virus - drug effects</topic><topic>Influenza A virus - genetics</topic><topic>Influenza A virus - isolation & purification</topic><topic>Influenza A Virus, H1N1 Subtype - drug effects</topic><topic>Influenza A Virus, H1N1 Subtype - genetics</topic><topic>Influenza B virus - drug effects</topic><topic>Influenza B virus - genetics</topic><topic>Influenza B virus - isolation & purification</topic><topic>Influenza, Human - epidemiology</topic><topic>Influenza, Human - virology</topic><topic>Medical research</topic><topic>Medical treatment</topic><topic>Morbidity - trends</topic><topic>Mutation, Missense</topic><topic>Neuraminidase - antagonists & inhibitors</topic><topic>Neuraminidase - genetics</topic><topic>Oseltamivir - pharmacology</topic><topic>Point Mutation</topic><topic>Population Surveillance</topic><topic>Public health</topic><topic>Resistance</topic><topic>Seasons</topic><topic>Virus Cultivation</topic><topic>Zanamivir - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pontoriero, Andrea</creatorcontrib><creatorcontrib>Baumeister, Elsa</creatorcontrib><creatorcontrib>Campos, Ana M</creatorcontrib><creatorcontrib>Savy, Vilma L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>International Bibliography of the Social Sciences (IBSS)</collection><collection>International Bibliography of the Social Sciences</collection><collection>International Bibliography of the Social Sciences</collection><collection>MEDLINE - Academic</collection><collection>SciELO</collection><jtitle>Revista panamericana de salud pública</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pontoriero, Andrea</au><au>Baumeister, Elsa</au><au>Campos, Ana M</au><au>Savy, Vilma L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008</atitle><jtitle>Revista panamericana de salud pública</jtitle><addtitle>Rev Panam Salud Publica</addtitle><date>2011-12</date><risdate>2011</risdate><volume>30</volume><issue>6</issue><spage>634</spage><epage>640</epage><pages>634-640</pages><issn>1020-4989</issn><issn>1680-5348</issn><eissn>1680-5348</eissn><abstract>To describe the virological characteristics of the influenza strains circulating in Argentina in 2005-2008 and to assess the prevalence of antiviral resistance.
On the basis of their geographical spread and prevalence, influenza A and B isolates grown in Madin-Darby canine kidney cells were selected after antigenic and genomic characterization to be analyzed for antiviral resistance by enzymatic assay and pyrosequencing. Amantadine susceptibility was evaluated by pyrosequencing for known resistance markers on 45 strains of influenza A. Susceptibility to oseltamivir and zanamivir was evaluated by enzymatic assay of 67 influenza A and 46 influenza B strains, some of which were further analyzed by sequencing the neuraminidase gene.
Resistance to amantadine was observed only on A(H3N2) strains (29/33); all of them carried the mutation S31N in their M2 sequence. Oseltamivir resistance was observed in 12 (34.3%) of the 35 A(H1N1) strains from 2008; all of them carried the mutation H275Y in their neuraminidase sequence. All these viruses remained sensitive to zanamivir.
This study describes a high incidence of amantadine-resistant influenza A(H3N2) viruses since 2006 and an unprecedented increase in oseltamivir resistance detected only in influenza A(H1N1) viruses isolated in 2008. Influenza A and B viruses were more sensitive to oseltamivir than to zanamivir, and influenza A viruses were more sensitive to both neuraminidase inhibitors than the influenza B viruses. The national data generated and analyzed in this study may help increase knowledge about influenza antiviral drug resistance, which is a problem of global concern.</abstract><cop>United States</cop><pub>Organización Panamericana de la Salud</pub><pmid>22358415</pmid><doi>10.1590/S1020-49892011001200023</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amantadine - pharmacology Animals Antiviral Agents - pharmacology Argentina Argentina - epidemiology Cell Line Data analysis Dogs Drug Resistance, Multiple, Viral - genetics Drug Resistance, Viral Drug use Epidemiology Health Policy & Services Hospitals Humans Influenza A virus - drug effects Influenza A virus - genetics Influenza A virus - isolation & purification Influenza A Virus, H1N1 Subtype - drug effects Influenza A Virus, H1N1 Subtype - genetics Influenza B virus - drug effects Influenza B virus - genetics Influenza B virus - isolation & purification Influenza, Human - epidemiology Influenza, Human - virology Medical research Medical treatment Morbidity - trends Mutation, Missense Neuraminidase - antagonists & inhibitors Neuraminidase - genetics Oseltamivir - pharmacology Point Mutation Population Surveillance Public health Resistance Seasons Virus Cultivation Zanamivir - pharmacology |
| title | Virological surveillance and antiviral resistance of human influenza virus in Argentina, 2005-2008 |
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