Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers
Leaf rust, caused by Puccinia triticina Eriks. is a common and widespread disease of bread wheat (Triticum aestivum L.), in Argentina. Host resistance is the most economical, effective and ecologically sustainable method of controlling the disease. Gene postulation helps to determine leaf rust resis...
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creator | Vanzetti, Leonardo Sebastián Campos, Pablo Demichelis, Melina Lombardo, Lucio Andres Aurelia, Paola Romina Vaschetto, Luis Maria Bainotti, Carlos Tomas Helguera, Marcelo |
description | Leaf rust, caused by Puccinia triticina Eriks. is a common and
widespread disease of bread wheat (Triticum aestivum L.), in Argentina.
Host resistance is the most economical, effective and ecologically
sustainable method of controlling the disease. Gene postulation helps
to determine leaf rust resistance genes (Lr genes) that may be present
in a large group of wheat germplasm. Additionally presence of Lr genes
can be determined using associated molecular markers. The objective of
this study was to identify Lr genes that condition leaf rust resistance
in 66 wheat cultivars from Argentina. Twenty four differential lines
with individual known leaf rust resistance genes were tested with 17
different pathotypes of leaf rust collected from Argentina. Leaf rust
infection types produced on seedling plants of the 66 local cultivars
were compared with the infection types produced by the same pathotypes
on Lr differentials to postulate which seedling leaf rust genes were
present. Presence of Lr9, Lr10, Lr19, Lr20, Lr21, Lr24, Lr25, Lr26,
Lr29, Lr34, Lr35, Lr37, Lr47 and Lr51 was also determined using
molecular markers. Eleven different Lr genes were postulated in the
material: Lr1, Lr3a, Lr3ka, Lr9, Lr10, Lr16, Lr17, Lr19, Lr24, Lr26,
Lr41. Presence of Lr21, Lr25, Lr29, and Lr47 could not be determined
with the seventeen pathotypes used in the study because all were
avirulent to these genes. Eleven cultivars (16.7%) were resistant to
all pathotypes used in the study and the remaining 55 (83.3%) showed
virulent reaction against one or more local pathotypes. Cultivars with
seedling resistance gene combinations including Lr16 or single genes
Lr47 (detected with molecular marker), Lr19 and Lr41, showed high
levels of resistance against all pathotypes or most of them. On the
opposite side, cultivars with seedling resistance genes Lr1, Lr3a, Lr3a
+ Lr24, Lr10, Lr3a + Lr10, Lr3a + Lr10 + Lr24 showed the highest number
of virulent reactions against local pathotypes. Occurrence of adult
plant resistance genes Lr34, Lr35i and Lr37 in local germplasm was
evaluated using specific molecular markers confirming presence of Lr34
and Lr37. Our data suggest that combinations including seedling
resistance genes like Lr16, Lr47, Lr19, Lr41, Lr21, Lr25 and Lr29, with
adult plant resistance genes like Lr34, SV2, Lr46 will probably provide
durable and effective resistance to leaf rust in the region. |
doi_str_mv | 10.2225/vol14-issue3-fulltext-14 |
format | Article |
fullrecord | <record><control><sourceid>scielo_bioli</sourceid><recordid>TN_cdi_scielo_journals_S0717_34582011000300009</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><scielo_id>S0717_34582011000300009</scielo_id><sourcerecordid>S0717_34582011000300009</sourcerecordid><originalsourceid>FETCH-LOGICAL-b233t-dffe60ed7c235e42c6620bef18258d9297170d5a309e490f386c4959cb5bd7b3</originalsourceid><addsrcrecordid>eNpNUNtKAzEQDaJgrf5DfmBrNsneHkvxUhB8sO9LLpOammZLkq323Q83tYrCDDMcOGfmHIRwSWaU0up2P7iSFzbGEVhhRucSfKSi5GdoQpqyKRiv2vN_-yW6inFDCCW84RP0udTgkzVWiWQHjweDHQiDwxgTDhBtTMIrwGvwELH1OIIDlUDjeVgfmR6ExzKA0Pj9FUTCanTJ7kWIWB6-aXg3xDS6k77wGm-HLJGBgLcivEGI1-jCCBfh5mdO0er-brV4LJ6eH5aL-VMhKWOp0MZATUA3irIKOFV1TYkEU7a0anVHu-yR6Eow0gHviGFtrXhXdUpWUjeSTdHsJBuVBTf0m2EMPt_rX47p9Md0KClLQgjLTbo_grSDy077XbD55UOvghX9LwibXJnGKPsCtop80A</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers</title><source>Bioline International</source><source>EZB-FREE-00999 freely available EZB journals</source><source>Free Full-Text Journals in Chemistry</source><creator>Vanzetti, Leonardo Sebastián ; Campos, Pablo ; Demichelis, Melina ; Lombardo, Lucio Andres ; Aurelia, Paola Romina ; Vaschetto, Luis Maria ; Bainotti, Carlos Tomas ; Helguera, Marcelo</creator><creatorcontrib>Vanzetti, Leonardo Sebastián ; Campos, Pablo ; Demichelis, Melina ; Lombardo, Lucio Andres ; Aurelia, Paola Romina ; Vaschetto, Luis Maria ; Bainotti, Carlos Tomas ; Helguera, Marcelo</creatorcontrib><description>Leaf rust, caused by Puccinia triticina Eriks. is a common and
widespread disease of bread wheat (Triticum aestivum L.), in Argentina.
Host resistance is the most economical, effective and ecologically
sustainable method of controlling the disease. Gene postulation helps
to determine leaf rust resistance genes (Lr genes) that may be present
in a large group of wheat germplasm. Additionally presence of Lr genes
can be determined using associated molecular markers. The objective of
this study was to identify Lr genes that condition leaf rust resistance
in 66 wheat cultivars from Argentina. Twenty four differential lines
with individual known leaf rust resistance genes were tested with 17
different pathotypes of leaf rust collected from Argentina. Leaf rust
infection types produced on seedling plants of the 66 local cultivars
were compared with the infection types produced by the same pathotypes
on Lr differentials to postulate which seedling leaf rust genes were
present. Presence of Lr9, Lr10, Lr19, Lr20, Lr21, Lr24, Lr25, Lr26,
Lr29, Lr34, Lr35, Lr37, Lr47 and Lr51 was also determined using
molecular markers. Eleven different Lr genes were postulated in the
material: Lr1, Lr3a, Lr3ka, Lr9, Lr10, Lr16, Lr17, Lr19, Lr24, Lr26,
Lr41. Presence of Lr21, Lr25, Lr29, and Lr47 could not be determined
with the seventeen pathotypes used in the study because all were
avirulent to these genes. Eleven cultivars (16.7%) were resistant to
all pathotypes used in the study and the remaining 55 (83.3%) showed
virulent reaction against one or more local pathotypes. Cultivars with
seedling resistance gene combinations including Lr16 or single genes
Lr47 (detected with molecular marker), Lr19 and Lr41, showed high
levels of resistance against all pathotypes or most of them. On the
opposite side, cultivars with seedling resistance genes Lr1, Lr3a, Lr3a
+ Lr24, Lr10, Lr3a + Lr10, Lr3a + Lr10 + Lr24 showed the highest number
of virulent reactions against local pathotypes. Occurrence of adult
plant resistance genes Lr34, Lr35i and Lr37 in local germplasm was
evaluated using specific molecular markers confirming presence of Lr34
and Lr37. Our data suggest that combinations including seedling
resistance genes like Lr16, Lr47, Lr19, Lr41, Lr21, Lr25 and Lr29, with
adult plant resistance genes like Lr34, SV2, Lr46 will probably provide
durable and effective resistance to leaf rust in the region.</description><identifier>ISSN: 0717-3458</identifier><identifier>EISSN: 0717-3458</identifier><identifier>DOI: 10.2225/vol14-issue3-fulltext-14</identifier><language>eng</language><publisher>Universidad Católica de Valparaíso</publisher><subject>BIOTECHNOLOGY & APPLIED MICROBIOLOGY ; genetic control of pathogens, molecular markers, plant breeding</subject><ispartof>Electronic Journal of Biotechnology, 2011-05, Vol.14 (3), p.9-9</ispartof><rights>Copyright 2011 by Universidad Católica de Valparaíso -- Chile</rights><rights>This work is licensed under a Creative Commons Attribution 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925,79426</link.rule.ids></links><search><creatorcontrib>Vanzetti, Leonardo Sebastián</creatorcontrib><creatorcontrib>Campos, Pablo</creatorcontrib><creatorcontrib>Demichelis, Melina</creatorcontrib><creatorcontrib>Lombardo, Lucio Andres</creatorcontrib><creatorcontrib>Aurelia, Paola Romina</creatorcontrib><creatorcontrib>Vaschetto, Luis Maria</creatorcontrib><creatorcontrib>Bainotti, Carlos Tomas</creatorcontrib><creatorcontrib>Helguera, Marcelo</creatorcontrib><title>Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers</title><title>Electronic Journal of Biotechnology</title><addtitle>Electron. J. Biotechnol</addtitle><description>Leaf rust, caused by Puccinia triticina Eriks. is a common and
widespread disease of bread wheat (Triticum aestivum L.), in Argentina.
Host resistance is the most economical, effective and ecologically
sustainable method of controlling the disease. Gene postulation helps
to determine leaf rust resistance genes (Lr genes) that may be present
in a large group of wheat germplasm. Additionally presence of Lr genes
can be determined using associated molecular markers. The objective of
this study was to identify Lr genes that condition leaf rust resistance
in 66 wheat cultivars from Argentina. Twenty four differential lines
with individual known leaf rust resistance genes were tested with 17
different pathotypes of leaf rust collected from Argentina. Leaf rust
infection types produced on seedling plants of the 66 local cultivars
were compared with the infection types produced by the same pathotypes
on Lr differentials to postulate which seedling leaf rust genes were
present. Presence of Lr9, Lr10, Lr19, Lr20, Lr21, Lr24, Lr25, Lr26,
Lr29, Lr34, Lr35, Lr37, Lr47 and Lr51 was also determined using
molecular markers. Eleven different Lr genes were postulated in the
material: Lr1, Lr3a, Lr3ka, Lr9, Lr10, Lr16, Lr17, Lr19, Lr24, Lr26,
Lr41. Presence of Lr21, Lr25, Lr29, and Lr47 could not be determined
with the seventeen pathotypes used in the study because all were
avirulent to these genes. Eleven cultivars (16.7%) were resistant to
all pathotypes used in the study and the remaining 55 (83.3%) showed
virulent reaction against one or more local pathotypes. Cultivars with
seedling resistance gene combinations including Lr16 or single genes
Lr47 (detected with molecular marker), Lr19 and Lr41, showed high
levels of resistance against all pathotypes or most of them. On the
opposite side, cultivars with seedling resistance genes Lr1, Lr3a, Lr3a
+ Lr24, Lr10, Lr3a + Lr10, Lr3a + Lr10 + Lr24 showed the highest number
of virulent reactions against local pathotypes. Occurrence of adult
plant resistance genes Lr34, Lr35i and Lr37 in local germplasm was
evaluated using specific molecular markers confirming presence of Lr34
and Lr37. Our data suggest that combinations including seedling
resistance genes like Lr16, Lr47, Lr19, Lr41, Lr21, Lr25 and Lr29, with
adult plant resistance genes like Lr34, SV2, Lr46 will probably provide
durable and effective resistance to leaf rust in the region.</description><subject>BIOTECHNOLOGY & APPLIED MICROBIOLOGY</subject><subject>genetic control of pathogens, molecular markers, plant breeding</subject><issn>0717-3458</issn><issn>0717-3458</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>RBI</sourceid><recordid>eNpNUNtKAzEQDaJgrf5DfmBrNsneHkvxUhB8sO9LLpOammZLkq323Q83tYrCDDMcOGfmHIRwSWaU0up2P7iSFzbGEVhhRucSfKSi5GdoQpqyKRiv2vN_-yW6inFDCCW84RP0udTgkzVWiWQHjweDHQiDwxgTDhBtTMIrwGvwELH1OIIDlUDjeVgfmR6ExzKA0Pj9FUTCanTJ7kWIWB6-aXg3xDS6k77wGm-HLJGBgLcivEGI1-jCCBfh5mdO0er-brV4LJ6eH5aL-VMhKWOp0MZATUA3irIKOFV1TYkEU7a0anVHu-yR6Eow0gHviGFtrXhXdUpWUjeSTdHsJBuVBTf0m2EMPt_rX47p9Md0KClLQgjLTbo_grSDy077XbD55UOvghX9LwibXJnGKPsCtop80A</recordid><startdate>20110515</startdate><enddate>20110515</enddate><creator>Vanzetti, Leonardo Sebastián</creator><creator>Campos, Pablo</creator><creator>Demichelis, Melina</creator><creator>Lombardo, Lucio Andres</creator><creator>Aurelia, Paola Romina</creator><creator>Vaschetto, Luis Maria</creator><creator>Bainotti, Carlos Tomas</creator><creator>Helguera, Marcelo</creator><general>Universidad Católica de Valparaíso</general><general>Pontificia Universidad Católica de Valparaíso</general><scope>RBI</scope><scope>GPN</scope></search><sort><creationdate>20110515</creationdate><title>Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers</title><author>Vanzetti, Leonardo Sebastián ; Campos, Pablo ; Demichelis, Melina ; Lombardo, Lucio Andres ; Aurelia, Paola Romina ; Vaschetto, Luis Maria ; Bainotti, Carlos Tomas ; Helguera, Marcelo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-b233t-dffe60ed7c235e42c6620bef18258d9297170d5a309e490f386c4959cb5bd7b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>BIOTECHNOLOGY & APPLIED MICROBIOLOGY</topic><topic>genetic control of pathogens, molecular markers, plant breeding</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vanzetti, Leonardo Sebastián</creatorcontrib><creatorcontrib>Campos, Pablo</creatorcontrib><creatorcontrib>Demichelis, Melina</creatorcontrib><creatorcontrib>Lombardo, Lucio Andres</creatorcontrib><creatorcontrib>Aurelia, Paola Romina</creatorcontrib><creatorcontrib>Vaschetto, Luis Maria</creatorcontrib><creatorcontrib>Bainotti, Carlos Tomas</creatorcontrib><creatorcontrib>Helguera, Marcelo</creatorcontrib><collection>Bioline International</collection><collection>SciELO</collection><jtitle>Electronic Journal of Biotechnology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vanzetti, Leonardo Sebastián</au><au>Campos, Pablo</au><au>Demichelis, Melina</au><au>Lombardo, Lucio Andres</au><au>Aurelia, Paola Romina</au><au>Vaschetto, Luis Maria</au><au>Bainotti, Carlos Tomas</au><au>Helguera, Marcelo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers</atitle><jtitle>Electronic Journal of Biotechnology</jtitle><addtitle>Electron. J. Biotechnol</addtitle><date>2011-05-15</date><risdate>2011</risdate><volume>14</volume><issue>3</issue><spage>9</spage><epage>9</epage><pages>9-9</pages><issn>0717-3458</issn><eissn>0717-3458</eissn><abstract>Leaf rust, caused by Puccinia triticina Eriks. is a common and
widespread disease of bread wheat (Triticum aestivum L.), in Argentina.
Host resistance is the most economical, effective and ecologically
sustainable method of controlling the disease. Gene postulation helps
to determine leaf rust resistance genes (Lr genes) that may be present
in a large group of wheat germplasm. Additionally presence of Lr genes
can be determined using associated molecular markers. The objective of
this study was to identify Lr genes that condition leaf rust resistance
in 66 wheat cultivars from Argentina. Twenty four differential lines
with individual known leaf rust resistance genes were tested with 17
different pathotypes of leaf rust collected from Argentina. Leaf rust
infection types produced on seedling plants of the 66 local cultivars
were compared with the infection types produced by the same pathotypes
on Lr differentials to postulate which seedling leaf rust genes were
present. Presence of Lr9, Lr10, Lr19, Lr20, Lr21, Lr24, Lr25, Lr26,
Lr29, Lr34, Lr35, Lr37, Lr47 and Lr51 was also determined using
molecular markers. Eleven different Lr genes were postulated in the
material: Lr1, Lr3a, Lr3ka, Lr9, Lr10, Lr16, Lr17, Lr19, Lr24, Lr26,
Lr41. Presence of Lr21, Lr25, Lr29, and Lr47 could not be determined
with the seventeen pathotypes used in the study because all were
avirulent to these genes. Eleven cultivars (16.7%) were resistant to
all pathotypes used in the study and the remaining 55 (83.3%) showed
virulent reaction against one or more local pathotypes. Cultivars with
seedling resistance gene combinations including Lr16 or single genes
Lr47 (detected with molecular marker), Lr19 and Lr41, showed high
levels of resistance against all pathotypes or most of them. On the
opposite side, cultivars with seedling resistance genes Lr1, Lr3a, Lr3a
+ Lr24, Lr10, Lr3a + Lr10, Lr3a + Lr10 + Lr24 showed the highest number
of virulent reactions against local pathotypes. Occurrence of adult
plant resistance genes Lr34, Lr35i and Lr37 in local germplasm was
evaluated using specific molecular markers confirming presence of Lr34
and Lr37. Our data suggest that combinations including seedling
resistance genes like Lr16, Lr47, Lr19, Lr41, Lr21, Lr25 and Lr29, with
adult plant resistance genes like Lr34, SV2, Lr46 will probably provide
durable and effective resistance to leaf rust in the region.</abstract><pub>Universidad Católica de Valparaíso</pub><doi>10.2225/vol14-issue3-fulltext-14</doi><tpages>1</tpages><oa>free_for_read</oa></addata></record> |
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source | Bioline International; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
subjects | BIOTECHNOLOGY & APPLIED MICROBIOLOGY genetic control of pathogens, molecular markers, plant breeding |
title | Identification of leaf rust resistance genes in selected Argentinean bread wheat cultivars by gene postulation and molecular markers |
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