Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects
A method for counting Infectious pancreatic necrosis virus (IPNV) through epifluorescence microscopy was analyzed in detail. Image processing and statistic considerations are included. The particle size of viruses was compared in different experimental conditions such as the staining of the virus wi...
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| Veröffentlicht in: | Electronic Journal of Biotechnology 2011-01, Vol.14 (1), p.11-12 |
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| container_title | Electronic Journal of Biotechnology |
| container_volume | 14 |
| creator | Pavlov, Maria Soledad Eissler, Yoanna Espinoza, Juan Carlos Garland, David E Kuznar, Juan |
| description | A method for counting Infectious pancreatic necrosis virus (IPNV)
through epifluorescence microscopy was analyzed in detail. Image
processing and statistic considerations are included. The particle size
of viruses was compared in different experimental conditions such as
the staining of the virus with SYBR-Green I or with antibodies for
specific fluorescence labeling of viral proteins. The type of surface
used as mounting support was assayed as well. The results indicated
that the most suitable method involves the mounting of the
viral-containing suspension on a membrane filter followed by the
staining with a monoclonal antibody specific for a viral protein
combined with a FITC (fluorescein isothiocyanate)-conjugated secondary
antibody. |
| doi_str_mv | 10.2225/vol14-issue1-fulltext-11 |
| format | Article |
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processing and statistic considerations are included. The particle size
of viruses was compared in different experimental conditions such as
the staining of the virus with SYBR-Green I or with antibodies for
specific fluorescence labeling of viral proteins. The type of surface
used as mounting support was assayed as well. The results indicated
that the most suitable method involves the mounting of the
viral-containing suspension on a membrane filter followed by the
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through epifluorescence microscopy was analyzed in detail. Image
processing and statistic considerations are included. The particle size
of viruses was compared in different experimental conditions such as
the staining of the virus with SYBR-Green I or with antibodies for
specific fluorescence labeling of viral proteins. The type of surface
used as mounting support was assayed as well. The results indicated
that the most suitable method involves the mounting of the
viral-containing suspension on a membrane filter followed by the
staining with a monoclonal antibody specific for a viral protein
combined with a FITC (fluorescein isothiocyanate)-conjugated secondary
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through epifluorescence microscopy was analyzed in detail. Image
processing and statistic considerations are included. The particle size
of viruses was compared in different experimental conditions such as
the staining of the virus with SYBR-Green I or with antibodies for
specific fluorescence labeling of viral proteins. The type of surface
used as mounting support was assayed as well. The results indicated
that the most suitable method involves the mounting of the
viral-containing suspension on a membrane filter followed by the
staining with a monoclonal antibody specific for a viral protein
combined with a FITC (fluorescein isothiocyanate)-conjugated secondary
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| source | Bioline International; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry |
| subjects | Biología BIOTECHNOLOGY & APPLIED MICROBIOLOGY digital image analysis, fluorescence microscope methods, immunofluorescence, Infectious pancreatic necrosis virus (IPNV), SYBR-Green I |
| title | Infectious pancreatic necrosis virus (IPNV) enumeration through epifluorescence microscopy: technical aspects |
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