Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration
The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days a...
Gespeichert in:
| Veröffentlicht in: | Biocell 2012-04, Vol.36 (1), p.31-36 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 36 |
|---|---|
| container_issue | 1 |
| container_start_page | 31 |
| container_title | Biocell |
| container_volume | 36 |
| creator | Surenciski, Mauro Rodrigo Flachsland, Eduardo Alberto Terada, Graciela Mroginski, Luis Amado Rey, Hebe Yolanda |
| description | The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30 degrees C for 1 min, rehydrated using the same liquid mediums [0.75 M sucrose (24 h), 0.5 M sucrose (24 h), 0.25 M sucrose (48 h) and 0.15 M sucrose (24 h)] and cultivated in half strength Murashige & Skoog medium (1962) with the addition of 2 g/L activated charcoal. Sixty four percent of seeds survived and developed into acclimatized plants after being cryopreserved. In this work, the encapsulation-dehydration technique was employed for first time in Cyrtopodium hatschbachii. |
| doi_str_mv | 10.32604/biocell.2012.36.031 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_sciel</sourceid><recordid>TN_cdi_scielo_journals_S0327_95452012000100002</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><scielo_id>S0327_95452012000100002</scielo_id><sourcerecordid>1197485219</sourcerecordid><originalsourceid>FETCH-LOGICAL-c420t-e59b4d227d34197afc43f50567cb6252ac42e0501128ba60fe491bbb6cb879ff3</originalsourceid><addsrcrecordid>eNpdkUtr3DAUhbVoaB7tPyhFkE26sHv1ssfLMCRtIJBC27WQ5GtGwR45kh3wv688M80iCyEJvnN0dQ4hXxiUglcgv1sfHPZ9yYHxUlQlCPaBXIDgddEoqc7JZUrPABKkYB_JOResFgL4Bem3cQljxITx1Uw-7Gno6HaJUxhD6-eB7syU3M4at_Oe_jI2TfTmKeZbaxwa_Eb9MJhpjkgTYpuoXSjunRnT3B_8ihZ3SxsP50_krDN9ws-n_Yr8vb_7s_1ZPD79eNjePhZOcpgKVI2VLed1KyRratM5KToFqqqdrbjiJmMIChjjG2sq6FA2zFpbObupm64TV6Q8-ibnsQ_6Ocxxnx_Uv9dE9JrIGhQAsLyAZ8HNUTDG8DJjmvTg0xqo2WOYk2Z5DLlRnDUZvX6Hvrlz0dRc5S9sMiWPlIshpYidHqMfTFw0A32oTJ8q0-skWlQ6V5ZlX0_msx2wfRP970v8A8sFlGU</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2397254208</pqid></control><display><type>article</type><title>Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration</title><source>MEDLINE</source><source>Tech Science Press</source><source>EZB-FREE-00999 freely available EZB journals</source><creator>Surenciski, Mauro Rodrigo ; Flachsland, Eduardo Alberto ; Terada, Graciela ; Mroginski, Luis Amado ; Rey, Hebe Yolanda</creator><creatorcontrib>Surenciski, Mauro Rodrigo ; Flachsland, Eduardo Alberto ; Terada, Graciela ; Mroginski, Luis Amado ; Rey, Hebe Yolanda</creatorcontrib><description>The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30 degrees C for 1 min, rehydrated using the same liquid mediums [0.75 M sucrose (24 h), 0.5 M sucrose (24 h), 0.25 M sucrose (48 h) and 0.15 M sucrose (24 h)] and cultivated in half strength Murashige & Skoog medium (1962) with the addition of 2 g/L activated charcoal. Sixty four percent of seeds survived and developed into acclimatized plants after being cryopreserved. In this work, the encapsulation-dehydration technique was employed for first time in Cyrtopodium hatschbachii.</description><identifier>ISSN: 0327-9545</identifier><identifier>ISSN: 1667-5746</identifier><identifier>DOI: 10.32604/biocell.2012.36.031</identifier><identifier>PMID: 23173302</identifier><language>eng</language><publisher>Argentina: Tech Science Press</publisher><subject>Activated carbon ; Alginic acid ; BIOLOGY ; Calcium alginate ; Charcoal ; Cryopreservation ; Cryopreservation - methods ; Cryoprotective Agents - pharmacology ; Cyrtopodium ; Dehydration ; Encapsulation ; Orchidaceae - drug effects ; Orchidaceae - growth & development ; Pollination ; Regeneration ; Seeds ; Seeds - drug effects ; Seeds - growth & development ; Sucrose</subject><ispartof>Biocell, 2012-04, Vol.36 (1), p.31-36</ispartof><rights>2012. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c420t-e59b4d227d34197afc43f50567cb6252ac42e0501128ba60fe491bbb6cb879ff3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,780,784,885,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23173302$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Surenciski, Mauro Rodrigo</creatorcontrib><creatorcontrib>Flachsland, Eduardo Alberto</creatorcontrib><creatorcontrib>Terada, Graciela</creatorcontrib><creatorcontrib>Mroginski, Luis Amado</creatorcontrib><creatorcontrib>Rey, Hebe Yolanda</creatorcontrib><title>Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration</title><title>Biocell</title><addtitle>Biocell</addtitle><description>The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30 degrees C for 1 min, rehydrated using the same liquid mediums [0.75 M sucrose (24 h), 0.5 M sucrose (24 h), 0.25 M sucrose (48 h) and 0.15 M sucrose (24 h)] and cultivated in half strength Murashige & Skoog medium (1962) with the addition of 2 g/L activated charcoal. Sixty four percent of seeds survived and developed into acclimatized plants after being cryopreserved. In this work, the encapsulation-dehydration technique was employed for first time in Cyrtopodium hatschbachii.</description><subject>Activated carbon</subject><subject>Alginic acid</subject><subject>BIOLOGY</subject><subject>Calcium alginate</subject><subject>Charcoal</subject><subject>Cryopreservation</subject><subject>Cryopreservation - methods</subject><subject>Cryoprotective Agents - pharmacology</subject><subject>Cyrtopodium</subject><subject>Dehydration</subject><subject>Encapsulation</subject><subject>Orchidaceae - drug effects</subject><subject>Orchidaceae - growth & development</subject><subject>Pollination</subject><subject>Regeneration</subject><subject>Seeds</subject><subject>Seeds - drug effects</subject><subject>Seeds - growth & development</subject><subject>Sucrose</subject><issn>0327-9545</issn><issn>1667-5746</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNpdkUtr3DAUhbVoaB7tPyhFkE26sHv1ssfLMCRtIJBC27WQ5GtGwR45kh3wv688M80iCyEJvnN0dQ4hXxiUglcgv1sfHPZ9yYHxUlQlCPaBXIDgddEoqc7JZUrPABKkYB_JOResFgL4Bem3cQljxITx1Uw-7Gno6HaJUxhD6-eB7syU3M4at_Oe_jI2TfTmKeZbaxwa_Eb9MJhpjkgTYpuoXSjunRnT3B_8ihZ3SxsP50_krDN9ws-n_Yr8vb_7s_1ZPD79eNjePhZOcpgKVI2VLed1KyRratM5KToFqqqdrbjiJmMIChjjG2sq6FA2zFpbObupm64TV6Q8-ibnsQ_6Ocxxnx_Uv9dE9JrIGhQAsLyAZ8HNUTDG8DJjmvTg0xqo2WOYk2Z5DLlRnDUZvX6Hvrlz0dRc5S9sMiWPlIshpYidHqMfTFw0A32oTJ8q0-skWlQ6V5ZlX0_msx2wfRP970v8A8sFlGU</recordid><startdate>20120401</startdate><enddate>20120401</enddate><creator>Surenciski, Mauro Rodrigo</creator><creator>Flachsland, Eduardo Alberto</creator><creator>Terada, Graciela</creator><creator>Mroginski, Luis Amado</creator><creator>Rey, Hebe Yolanda</creator><general>Tech Science Press</general><general>Sociedad Latinoamericana de Microscopía Electrónica.; Centro Regional de Investigaciones Científicas y Tecnológicas (Mendoza, Argentina)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FE</scope><scope>8FH</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>GPN</scope></search><sort><creationdate>20120401</creationdate><title>Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration</title><author>Surenciski, Mauro Rodrigo ; Flachsland, Eduardo Alberto ; Terada, Graciela ; Mroginski, Luis Amado ; Rey, Hebe Yolanda</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c420t-e59b4d227d34197afc43f50567cb6252ac42e0501128ba60fe491bbb6cb879ff3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Activated carbon</topic><topic>Alginic acid</topic><topic>BIOLOGY</topic><topic>Calcium alginate</topic><topic>Charcoal</topic><topic>Cryopreservation</topic><topic>Cryopreservation - methods</topic><topic>Cryoprotective Agents - pharmacology</topic><topic>Cyrtopodium</topic><topic>Dehydration</topic><topic>Encapsulation</topic><topic>Orchidaceae - drug effects</topic><topic>Orchidaceae - growth & development</topic><topic>Pollination</topic><topic>Regeneration</topic><topic>Seeds</topic><topic>Seeds - drug effects</topic><topic>Seeds - growth & development</topic><topic>Sucrose</topic><toplevel>online_resources</toplevel><creatorcontrib>Surenciski, Mauro Rodrigo</creatorcontrib><creatorcontrib>Flachsland, Eduardo Alberto</creatorcontrib><creatorcontrib>Terada, Graciela</creatorcontrib><creatorcontrib>Mroginski, Luis Amado</creatorcontrib><creatorcontrib>Rey, Hebe Yolanda</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Biological Science Database</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>SciELO</collection><jtitle>Biocell</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Surenciski, Mauro Rodrigo</au><au>Flachsland, Eduardo Alberto</au><au>Terada, Graciela</au><au>Mroginski, Luis Amado</au><au>Rey, Hebe Yolanda</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration</atitle><jtitle>Biocell</jtitle><addtitle>Biocell</addtitle><date>2012-04-01</date><risdate>2012</risdate><volume>36</volume><issue>1</issue><spage>31</spage><epage>36</epage><pages>31-36</pages><issn>0327-9545</issn><issn>1667-5746</issn><abstract>The aim of the present study was to investigate the efficiency of the encapsulation-dehydration technique for cryopreservation of Cyrtopodium hastchbachii Pabst seeds. Immature seeds of this species were cryopreserved by an encapsulation-dehydration technique. Seeds of five immature pods, 120 days after pollination, were encapsulated in 3% calcium alginate matrix and pretreated in liquid medium supplemented with 0.08 M sucrose (24 h), 0.15 M sucrose (24 h), 0.25 M sucrose (48 h), 0.5 M sucrose (24 h) and 0.75 M sucrose (24 h) in shaker at 60 rpm. Alginate beads were dehydrated 5 h in silicagel and immersed in liquid nitrogen for 12 h. Cryopreserved beads were thawed at 30 degrees C for 1 min, rehydrated using the same liquid mediums [0.75 M sucrose (24 h), 0.5 M sucrose (24 h), 0.25 M sucrose (48 h) and 0.15 M sucrose (24 h)] and cultivated in half strength Murashige & Skoog medium (1962) with the addition of 2 g/L activated charcoal. Sixty four percent of seeds survived and developed into acclimatized plants after being cryopreserved. In this work, the encapsulation-dehydration technique was employed for first time in Cyrtopodium hatschbachii.</abstract><cop>Argentina</cop><pub>Tech Science Press</pub><pmid>23173302</pmid><doi>10.32604/biocell.2012.36.031</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0327-9545 |
| ispartof | Biocell, 2012-04, Vol.36 (1), p.31-36 |
| issn | 0327-9545 1667-5746 |
| language | eng |
| recordid | cdi_scielo_journals_S0327_95452012000100002 |
| source | MEDLINE; Tech Science Press; EZB-FREE-00999 freely available EZB journals |
| subjects | Activated carbon Alginic acid BIOLOGY Calcium alginate Charcoal Cryopreservation Cryopreservation - methods Cryoprotective Agents - pharmacology Cyrtopodium Dehydration Encapsulation Orchidaceae - drug effects Orchidaceae - growth & development Pollination Regeneration Seeds Seeds - drug effects Seeds - growth & development Sucrose |
| title | Cryopreservation of Cyrtopodium hatschbachii Pabst (Orchidaceae) immature seeds by encapsulation-dehydration |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-26T03%3A13%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_sciel&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cryopreservation%20of%20Cyrtopodium%20hatschbachii%20Pabst%20(Orchidaceae)%20immature%20seeds%20by%20encapsulation-dehydration&rft.jtitle=Biocell&rft.au=Surenciski,%20Mauro%20Rodrigo&rft.date=2012-04-01&rft.volume=36&rft.issue=1&rft.spage=31&rft.epage=36&rft.pages=31-36&rft.issn=0327-9545&rft_id=info:doi/10.32604/biocell.2012.36.031&rft_dat=%3Cproquest_sciel%3E1197485219%3C/proquest_sciel%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=2397254208&rft_id=info:pmid/23173302&rft_scielo_id=S0327_95452012000100002&rfr_iscdi=true |