Genome-wide identification and expression analysis of JmjC domain-containing gene family related to abiotic stress and photoperiodic treatments in Mung bean (Vignaradiata L.)

Although the JmjC domain-containing histone demethylases displayed a crucial role in maintaining the homeostasis of histone methylation, while the systematic identification and functional researches of JmjC domain-containing gene family have not been conducted in Mung bean (VrJMJgenes). According to the structural characteristics and phylogenetic relationship with their orthologs from Glycine max, Lotus japonicus, Medicagotruncatula, Arabidopsis thaliana, and Oryza sativa, a total of 18 VrJMJgenes were identified and divided into four clades (KDM3, KDM5. PKDM8, and PKDM9). Interspecies co-collinearity analysis showed the significant JmjC gene duplication events which have occurred during the Papilionoideae evolution. The exon/intron and domain organization of VrJMJgenes from the same clade (or subclade) were similar. All VrJMJ proteins contained a conserved JmjC domain, meanwhile other essential domains also have been found in some specific VrJMJ proteins which responsible for their functions. Numerous abiotic stress and light response related cis-elements associating with transcriptional regulation that were demonstrated in the promoter regions of VrJMJgenes(Pro VrJMJs ). Expression profiles of VrJMJgenes in different tissues showed that most genes displayed a tissue-specific expression in roots or leaves. The acronym RT-qPCR results showed that all VrJMJ genes displayed different degrees of abiotic stress (drought, salinity, and cold) and photoperiodic responses. Furthermore, VrJMJ3 and VrJMJ9 were significantly up-regulated after all three abiotic stress treatments, and VrJMJ13 exhibited a potential function in the photoperiodic regulation of Mung bean flowering. These results provided a clear understanding of VrJMJ genes, and laid a theoretical basis for further verification of their potential biological functions of VrJMJ genes. RESUMO: Embora as desmetilases de histonas contendo o domínio JmjC exibam um papel crucial na manutenção da homeostase das metilações de histonas, enquanto a identificação sistemática e a pesquisa funcional da família de genes contendo o domínio JmjC não foram conduzidas em feijão mungo (genes VrJMJ). De acordo com suas características de estrutura e relações filogenéticas com os ortólogos de Glycine max, Lotus japonicus, Medicago truncatula, Arabidopsis thaliana e Oryza sativa, se identificaram um total de 18 genes VrJMJ se divididos em quatro clados (KDM3, KDM5, PKDM8 e PKDM9). A análise de colinearidade exibiu eventos signi