Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured
The effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Mediu...
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| Veröffentlicht in: | Arquivo brasileiro de medicina veterinária e zootecnia 2009-02, Vol.61 (1), p.57-65 |
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| creator | Lima-Verde, I.B. Matos, M.H.T. Bruno, J.B. Martins, F.S. Santos, R.R. Báo, S.N. Luque, M.C.A. Vieira, G.A.B. Silveira, E.R. Rodrigues, A.P.R. Figueiredo, J.R. Oliveira, M.A.L. Lima, P.F. |
| description | The effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without α-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15µM, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P |
| doi_str_mv | 10.1590/S0102-09352009000100009 |
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Os efeitos do α-tocoferol e da ternatina sobre morfologia, ativação e crescimento de folículos pré-antrais caprinos cultivados in vitro, por um ou cinco dias, foram avaliados. Os fragmentos ovarianos foram imediatamente fixados (controle não-cultivado) ou cultivados in vitro, por um ou cinco dias, em Meio Essencial Mínimo (MEM) com ou sem suplementação com α-tocoferol ou ternatina nas concentrações de 5, 10 ou 15µM, formando os tratamentos MEM, TOC5, TOC10, TOC 15, TER5, TER10, TER15. O percentual de folículos pré-antrais normais no controle não-cultivado foi de 73,2%, depois de cinco dias de cultivo, houve redução desse percentual em todos os tratamentos, quando comparados com o controle não-cultivado (P<0,05). O cultivo por cinco dias aumentou a ativação folicular em todos os tratamentos (P<0,05). A análise ultra-estrutural não mostrou folículos pré-antrais íntegros após cinco dias de cultivo em meio contendo antioxidantes. Concluiu-se que o α-tocoferol e a ternatina podem promover a ativação folicular, no entanto a adição desses antioxidantes nas concentrações testadas reduziu a viabilidade folicular após o cultivo in vitro.</description><identifier>ISSN: 0102-0935</identifier><identifier>ISSN: 1678-4162</identifier><identifier>EISSN: 0102-0935</identifier><identifier>DOI: 10.1590/S0102-09352009000100009</identifier><language>eng</language><publisher>Universidade Federal de Minas Gerais, Escola de Veterinária</publisher><subject>VETERINARY SCIENCES</subject><ispartof>Arquivo brasileiro de medicina veterinária e zootecnia, 2009-02, Vol.61 (1), p.57-65</ispartof><rights>This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c275t-bc596539d86be14b45f1386d5c41049b63d6e3550cbb7876f924471d796485d63</citedby><cites>FETCH-LOGICAL-c275t-bc596539d86be14b45f1386d5c41049b63d6e3550cbb7876f924471d796485d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>230,314,777,781,861,882,27905,27906</link.rule.ids></links><search><creatorcontrib>Lima-Verde, I.B.</creatorcontrib><creatorcontrib>Matos, M.H.T.</creatorcontrib><creatorcontrib>Bruno, J.B.</creatorcontrib><creatorcontrib>Martins, F.S.</creatorcontrib><creatorcontrib>Santos, R.R.</creatorcontrib><creatorcontrib>Báo, S.N.</creatorcontrib><creatorcontrib>Luque, M.C.A.</creatorcontrib><creatorcontrib>Vieira, G.A.B.</creatorcontrib><creatorcontrib>Silveira, E.R.</creatorcontrib><creatorcontrib>Rodrigues, A.P.R.</creatorcontrib><creatorcontrib>Figueiredo, J.R.</creatorcontrib><creatorcontrib>Oliveira, M.A.L.</creatorcontrib><creatorcontrib>Lima, P.F.</creatorcontrib><title>Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured</title><title>Arquivo brasileiro de medicina veterinária e zootecnia</title><addtitle>Arq. Bras. Med. Vet. Zootec</addtitle><description>The effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without α-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15µM, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P<0.05) when compared with non-cultured control. Culture of ovarian cortex for five days increased the percentages of follicular activation in all treatments (P<0.05). Ultrastructural analysis did not confirm the integrity of caprine preantral follicles cultured for five days in medium containing antioxidants. This study demonstrated that α-tocopherol and ternatin can promote follicular activation; however, addition of these antioxidants in the tested concentrations reduced the follicular viability after in vitro culture.
Os efeitos do α-tocoferol e da ternatina sobre morfologia, ativação e crescimento de folículos pré-antrais caprinos cultivados in vitro, por um ou cinco dias, foram avaliados. Os fragmentos ovarianos foram imediatamente fixados (controle não-cultivado) ou cultivados in vitro, por um ou cinco dias, em Meio Essencial Mínimo (MEM) com ou sem suplementação com α-tocoferol ou ternatina nas concentrações de 5, 10 ou 15µM, formando os tratamentos MEM, TOC5, TOC10, TOC 15, TER5, TER10, TER15. O percentual de folículos pré-antrais normais no controle não-cultivado foi de 73,2%, depois de cinco dias de cultivo, houve redução desse percentual em todos os tratamentos, quando comparados com o controle não-cultivado (P<0,05). O cultivo por cinco dias aumentou a ativação folicular em todos os tratamentos (P<0,05). A análise ultra-estrutural não mostrou folículos pré-antrais íntegros após cinco dias de cultivo em meio contendo antioxidantes. Concluiu-se que o α-tocoferol e a ternatina podem promover a ativação folicular, no entanto a adição desses antioxidantes nas concentrações testadas reduziu a viabilidade folicular após o cultivo in vitro.</description><subject>VETERINARY SCIENCES</subject><issn>0102-0935</issn><issn>1678-4162</issn><issn>0102-0935</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><recordid>eNp1UMtKAzEUDaJgrX6D-YGpyUySmSylVC0UXKjrIZNHm5JOhiQtduFH-SN-k7EVEcS7OfdyHnAPANcYTTDl6OYJYVQWiFe0RIgjlM88_ASMfojTX_s5uIhxjVBJOMcj8DYzRssUoTfw471IXvphpYN3UPQKJh16kWyfj2T9q1UZs7SHGx-GlXd-uT_ohEx2l4WZyTlLLxIcgs7iIBw03jkrnY4wB-1sCh7KrUvboNUlODPCRX31jWPwcjd7nj4Ui8f7-fR2UciypqnoJOWMVlw1rNOYdIQaXDVMUUkwIrxjlWK6ohTJrqubmhleElJjVXNGGqpYNQaTY26UVjvfrv02P-Zieyiv_VNeNtRHgww-xqBNOwS7EWHfYtR-1f6v8xP8U3WH</recordid><startdate>200902</startdate><enddate>200902</enddate><creator>Lima-Verde, I.B.</creator><creator>Matos, M.H.T.</creator><creator>Bruno, J.B.</creator><creator>Martins, F.S.</creator><creator>Santos, R.R.</creator><creator>Báo, S.N.</creator><creator>Luque, M.C.A.</creator><creator>Vieira, G.A.B.</creator><creator>Silveira, E.R.</creator><creator>Rodrigues, A.P.R.</creator><creator>Figueiredo, J.R.</creator><creator>Oliveira, M.A.L.</creator><creator>Lima, P.F.</creator><general>Universidade Federal de Minas Gerais, Escola de Veterinária</general><scope>AAYXX</scope><scope>CITATION</scope><scope>GPN</scope></search><sort><creationdate>200902</creationdate><title>Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured</title><author>Lima-Verde, I.B. ; Matos, M.H.T. ; Bruno, J.B. ; Martins, F.S. ; Santos, R.R. ; Báo, S.N. ; Luque, M.C.A. ; Vieira, G.A.B. ; Silveira, E.R. ; Rodrigues, A.P.R. ; Figueiredo, J.R. ; Oliveira, M.A.L. ; Lima, P.F.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c275t-bc596539d86be14b45f1386d5c41049b63d6e3550cbb7876f924471d796485d63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>VETERINARY SCIENCES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lima-Verde, I.B.</creatorcontrib><creatorcontrib>Matos, M.H.T.</creatorcontrib><creatorcontrib>Bruno, J.B.</creatorcontrib><creatorcontrib>Martins, F.S.</creatorcontrib><creatorcontrib>Santos, R.R.</creatorcontrib><creatorcontrib>Báo, S.N.</creatorcontrib><creatorcontrib>Luque, M.C.A.</creatorcontrib><creatorcontrib>Vieira, G.A.B.</creatorcontrib><creatorcontrib>Silveira, E.R.</creatorcontrib><creatorcontrib>Rodrigues, A.P.R.</creatorcontrib><creatorcontrib>Figueiredo, J.R.</creatorcontrib><creatorcontrib>Oliveira, M.A.L.</creatorcontrib><creatorcontrib>Lima, P.F.</creatorcontrib><collection>CrossRef</collection><collection>SciELO</collection><jtitle>Arquivo brasileiro de medicina veterinária e zootecnia</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lima-Verde, I.B.</au><au>Matos, M.H.T.</au><au>Bruno, J.B.</au><au>Martins, F.S.</au><au>Santos, R.R.</au><au>Báo, S.N.</au><au>Luque, M.C.A.</au><au>Vieira, G.A.B.</au><au>Silveira, E.R.</au><au>Rodrigues, A.P.R.</au><au>Figueiredo, J.R.</au><au>Oliveira, M.A.L.</au><au>Lima, P.F.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured</atitle><jtitle>Arquivo brasileiro de medicina veterinária e zootecnia</jtitle><addtitle>Arq. Bras. Med. Vet. Zootec</addtitle><date>2009-02</date><risdate>2009</risdate><volume>61</volume><issue>1</issue><spage>57</spage><epage>65</epage><pages>57-65</pages><issn>0102-0935</issn><issn>1678-4162</issn><eissn>0102-0935</eissn><abstract>The effects of α-tocopherol and ternatin on the morphology, activation, and growth of goat preantral follicles in vitro cultured, for one or five days, were evaluated. Ovarian fragments were immediately fixed (non-cultured control) or in vitro cultured for one or five days in Minimum Essential Medium (MEM) with or without α-tocopherol or ternatin supplementation, both at concentrations of 5, 10, or 15µM, corresponding to the following treatments: MEM, TOC5, TOC10, TOC 15, TER5, TER10, and TER15. The percentages of morphologically normal preantral follicles in non-cultured ovarian tissue (control) was 73.2% and after five days of culture, there was a decrease on these percentages in all treatments (P<0.05) when compared with non-cultured control. Culture of ovarian cortex for five days increased the percentages of follicular activation in all treatments (P<0.05). Ultrastructural analysis did not confirm the integrity of caprine preantral follicles cultured for five days in medium containing antioxidants. This study demonstrated that α-tocopherol and ternatin can promote follicular activation; however, addition of these antioxidants in the tested concentrations reduced the follicular viability after in vitro culture.
Os efeitos do α-tocoferol e da ternatina sobre morfologia, ativação e crescimento de folículos pré-antrais caprinos cultivados in vitro, por um ou cinco dias, foram avaliados. Os fragmentos ovarianos foram imediatamente fixados (controle não-cultivado) ou cultivados in vitro, por um ou cinco dias, em Meio Essencial Mínimo (MEM) com ou sem suplementação com α-tocoferol ou ternatina nas concentrações de 5, 10 ou 15µM, formando os tratamentos MEM, TOC5, TOC10, TOC 15, TER5, TER10, TER15. O percentual de folículos pré-antrais normais no controle não-cultivado foi de 73,2%, depois de cinco dias de cultivo, houve redução desse percentual em todos os tratamentos, quando comparados com o controle não-cultivado (P<0,05). O cultivo por cinco dias aumentou a ativação folicular em todos os tratamentos (P<0,05). A análise ultra-estrutural não mostrou folículos pré-antrais íntegros após cinco dias de cultivo em meio contendo antioxidantes. Concluiu-se que o α-tocoferol e a ternatina podem promover a ativação folicular, no entanto a adição desses antioxidantes nas concentrações testadas reduziu a viabilidade folicular após o cultivo in vitro.</abstract><pub>Universidade Federal de Minas Gerais, Escola de Veterinária</pub><doi>10.1590/S0102-09352009000100009</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| title | Effects of α-tocopherol and ternatin antioxidants on morphology and activation of goat preantral follicles in vitro cultured |
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